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Core Facilities

High-Throughput Polymorphism Detection

Pricing

 1) SNP Analysis using Taqman

SNP start up fees:

     ◦   Fewer than 700 samples and assay not in stock (DF/HCC): $258
     ◦   Fewer than 700 samples and assay not in stock: $295

     ◦   700-3500 samples and assay is in stock (DF/HCC): $420
     ◦   700-3500 samples and assay is in stock: $480

     ◦  3500 samples or more and assay not in stock (DF/HCC): $858
     ◦  3500 samples or more and assay not in stock: $980

Genotyping:
      ◦  Taqman assay (DF/HCC members): $0.60 per SNP per sample
      ◦  Taqman assay: $0.68 per SNP per sample

 2) SNP Analysis using Taqman OpenArray Genotyping

The Taqman OpenArray platform allows users to run 16, 32 or 64 SNPs in parallel using Taqman reagents.  Approximate pricing for DF/HCC members:

OpenArray set-up fee (per project): $241

# Samples

16

32

64

<350

0.51

0.30

0.30

<500

0.36

0.22

0.20

<1500

0.22

0.20

0.16

<2500

0.20

0.15

0.14

<3500

0.19

0.14

0.13

<4500

0.18

0.13

0.13

<5000

0.15

0.13

0.13

The per genotype cost varies with the number of samples and the number of SNPs.  Please contact Hardeep Ranu for exact pricing information.

3) Ion Torrent PGM

Targeted sequencing service using the Ion Torrent PGM. The capabilities of this service include:

Custom Amplicon Sequencing of DNA and RNA Panels

Ready-to-use Ion AmpliSeq Panels: 

Cancer Hotspot Panel  50 genes, > 2,800 Cosmic Mutations, 207 Amplicons
Comprehensive Cancer Panel  409 genes, 16,000 Amplicons
Inherited Disease Panel  700 disease, 328 genes, 10,000 Amplicons

The system requires only 10ng of input DNA and DNA from FFPE samples can also be used. Additionally, only one or two samples can be run at a time.

There are three different Chip formats:

  • Ion 314 Chip: 1 million wells,  400-550 thousand reads for 200bp sequencing
  • Ion 316 Chip: 6 million wells, 2-3 million reads for 200bp sequencing
  • Ion 318 Chip: 11 million wells, 4-5.5 million reads for 200bp sequencing

Please contact Hardeep Ranu for more information

4) DNA Extraction, plating, and whole genome amplification (all prices quoted are for DF/HCC members)    

    ◦    Sample plating: $1.49 per sample   

    ◦    Pico green DNA quantification: $0.88 per sample

    ◦    Full DNA extraction: $17.51 per sample

    ◦    Whole genome amplification: $15.76 per sample

    ◦    Sample DNA standardization (pico, dilution, tracking, submission: $3.50 per sample

Please contact Pati Soule for more information.

5) Telomere Length Analysis 

Please contact Pati Soule for more information on this service.  

6) SNP analysis and sequencing using Illumina

DF/HCC members receive member rates for these services. For general pricing information for this platform, please click here.

Please contact Ashley Blau for quotes and information on the Illumina platform.