Core Facilities

High-Throughput Polymorphism Detection

Pricing

1) SNP Analysis using Taqman

SNP start up fees:
     ◦ Fewer than 700 samples and assay not in stock: $250.00
     ◦ 700-2000 samples and assay is in stock: $400.00
    ◦ 700-2000 samples and assay not in stock: $465.00
    ◦ 2000 samples or more and assay not in stock: $905.00

Genotyping:
◦ Taqman assay: $.60 per sample
    ◦ Taqman assay (DF/HCC members): $.50 per sample

Sample Verification:

◦ Two SNP sample verification: $1.00 per sample

2) SNP Analysis using Taqman OpenArray Genotyping

The Taqman OpenArray platform allows users to run 16, 32 or 64 SNPs in parallel using Taqman reagents.

# Samples	16	32	64
<350	0.51	0.30	0.30
<500	0.27	0.16	0.16
<1500	0.22	0.20	0.16
<2500	0.20	0.15	0.14
<3500	0.19	0.14	0.13
<4500	0.18	0.13	0.13
<5000	0.15	0.13	0.13

The per genotype cost varies with the number of samples and the number of SNPs. Please contact Hardeep Ranu for exact pricing information.

617 432 4469 / hranu@hsph.harvard.edu

3) DNA Extraction, plating, and whole genome amplification
    ◦    Sample plating: $1.00 per sample
    ◦    Pico green DNA quantification: $1.00 per sample
    ◦    Full DNA extraction or whole genome amplification: $13.00 per sample;
    ◦    Full DNA extraction of Whole genome amplification (DF/HCC discounted rate): $11.00 per sample

◦ Specific DNA standardization (pico, dilution, tracking, submission): $5.00 per sample

◦ GWAS (Genome Wide Association Study) sample preparation (steps may include pico, dry-down to concentrate, resuspension, re-pico, track and submit): $10-15

Please contact Pati Soule for more information:

617 432 6101 / psoule@hsph.harvard.edu

4) SNP analysis using Sequenom

SNP start up fees:
◦ $50.00 per SNP assay ordered, cost covers assay design and oligo ordering, for anywhere from 100-2000 DNA samples

Genotyping:
For orders of more than 20 SNP: $0.35 per SNP-genotype. For DF/HCC members, $0.30 per SNP-genotype.

5) Genotyping using SNPlex, Illumina, and Affymetrix

◦ Please click here for more pricing information

Please contact Alison Brown for more information.

617-768-8470 / abrown13@partners.org