Core Facilities

Monoclonal Antibody

Service

The Monoclonal Antibody Core provides a wide variety of services from cloning and scale-up of immunization antigens to purification of the resulting antibodies. Researchers can choose to have all phases of antibody production performed by the Core or perform particular steps in their own lab.

Monoclonal Antibody Generation

Immunizations (Standard, RIMMS & Subtractive)
Peptides, Proteins, Cells & cDNA
Mice, Rats, Hamsters, Rabbits
Hybridoma fusions (PEG & Electrofusion)
Subcloning (Limiting Dilution)
Screening (ELISA, WB, FACS)

Polyclonal Antibody Generation

Immunization (Rabbits)
4 Production Bleeds
Phospho-specific antibodies
Affinity purification (Protein A or Antigen)

Recombinant Protein Production

Up to 5 gm production available using bioreactor
Productions performed in bacteria, mammalian or insect cell systems
Purifications available using all commonly used tags including His, GST, Strep or Fc fusion proteins.
Fast turnaround using our AKTA express systems.
Antibody production including low endotoxin production

Other Available Services

Antigen Design and Consultation
Cell Line Generation
Isotyping
Tagging of Antibodies
Production of Fab & F(ab’)2 ragments
Cell Pellet Production
Assay Development
Subcloning and Stabilization of Hybridomas
Cryopreservation

What sets us apart: MAC vs CRO

Service	Monoclonal Antibody Core	CRO
Immunization	3-5 mice (Multiple strains)	3-5 Balb/c mice
Types	Standard, RIMMS, Subtractive, cDNA	Standard, RIMMS
Fusion	PEG, Electrofusion	PEG
Screening	ELISA, WB, FACS, Custom	ELISA
# Hybridoma	All Positives	5 to 24
Subcloning	Individual basis, 3 clones/parental	3-5 hybridomas, 1-2 clones/parental
Deliverables	2 vials/subclone	2 vials/subclone
Other	Monoclonal Antibody Core	CRO
Project Consultation	In-depth	Cursory
Immunogenicity Screen	Software, structure	Software
Project design	Customized, Flexible	Set package
Experience	30+ years	<5 years
Relationship	Partnership/Collaborator	Hired service
Training	Provided upon request	None
Assay Development	As needed	None

Limited mAb catalog

Hybridoma	Target	Host	Isotype	Hybridoma	Target	Host	Isotype
9.49	hMHC class I	Mouse	IgG2a	HF2.3D1	hB7-2	Mouse	IgG2a
10-2.16	MHC class I (ant-IA k TIB93)	Mouse	IgG2b	IV.3.D10	CD32 (extracellular portion of FcRII)	Mouse	IgG2b
12CA5	HA	Mouse	IgG2b	J2	hCD9	Mouse	IgM
145-2C11	mCD3	Hamster	IgG	JRG.12	hCD40	Mouse	IgG1
16.10A1	mB7-1	Hamster	IgG	K20	B1 Integrin	Mouse	IgG2a
2.4G2	mFcR (FcR116/CD16-2)	Rat	IgG2b	L243	hIa	Mouse	IgG2a
3C10	h Monocytes	Mouse	IgG2b	LB3.1	HLA-DR	Mouse	IgG2b
3G8	CD16	Mouse	IgG1	M1 (TIB126)	anti-H-2	Rat	IgG2a
53-6.7 (TIB105)	mCD8	Rat	IgG2a	M5/114	IA/IE	Rat	IgG2b
9.00E+10	myc	Mouse	IgG1	MA2.1	HLA	Mouse	IgG1
9H10	mCTLA4	Hamster	IgG	MJ123.2B7	Telomerase	Mouse	IgM
B1	CD20	Mouse	IgG2a	Mo1	CD11b	Mouse	IgM
B4	CD19/CD20	Mouse	IgG1	Mo2	CD14	Mouse	IgM
BB7.1	HLA-B7	Mouse	IgG1	Mo2.D4	CD14	Mouse
BB7.2	HLA-A2	Mouse	IgG2b	MY9	CD33	Mouse	IgG2b
Clone 90	Reticulocytes (elf-2 90 Kd)	Mouse	IgG2a	N901	CD56	Mouse	IgG1
CT6-1D7	cytoplasmic tail of FcRI	Mouse	IgG1	PV1.17.10	mCD28	Hamster	IgG
DG122	Gst	Mouse	IgG1	T3-70	Mouse H-Y TcR (male Ag)	Mouse	IgG1
ER5.3D8	hCTLA4	Mouse	IgG2b	T4	hCD4	Mouse	IgG2a
EW3.4B2	hB7-1	Mouse	IgG2a	T8	hCD8	Mouse	IgG1
EX5.3D10	hCD28	Mouse	IgG2a	TMB1.4	IL12beta	Rat	IgG2b
GK1.5	mCD4	Rat	IgG2b	V30	vav	Mouse	IgG1
GL1	mB7-2	Rat	IgG2a	W6/32	HLA-A,B,C	Mouse	IgG2a
H57.597	mTcR	Hamster	IgG	Y3P	rat Ia	Mouse	IgG2a