To access the core, please contact Zhirui Wang at 617.643.1957 or by email at
zhirui.wang@tbrc.mgh.harvard.edu.

Yeast display vector: pYD1, pYD5, and yeast display strain: yeast Saccharomyces cerevisiae EBY100 are available in this core (redistribution of pYD1, pYD5, and EBY100 permitted by inventors) for worldwide research community.

Core publications:

Hermanrud CE, Lucas CL, Sykes M, Huang CA, Wang Z. (2011) Expression and purification of soluble murine CD40L monomers and polymers in yeast Pichia pastoris, Protein Expr Purif. 76:115-20.

Wang Z, Duran-Struuck R, Crepeau R, Matar A, Hanekamp I, Srinivasan S, Neville DM Jr, Sachs DH, Huang CA. (2011) Development of a diphtheria toxin based antiporcine CD3 recombinant immunotoxin, Bioconjug Chem. 22:2014-20.

Hermanrud CE, Pathiraja V, Matar A, Duran-Struuck R, Crepeau RL, Srinivasan S, Sachs DH, Huang CA, Wang Z. (2011) Expression and purification of non-N-glycosylated porcine interleukin 3 in yeast Pichia pastoris, Protein Expr Purif. 82:70-74.

Peraino J, Zhang H,  Hermanrud CE, Li G, Sachs DH, Huang CA, Wang Z. (2012) Expression and purification of soluble porcine CTLA-4 in yeast Pichia pastoris, Protein Expr Purif. 82:270-278.