Cat. #

Protein Name

Host

Price

RP1

Anti-NHP CD3 immunotoxin

P. pastoris

$900/mg

PP2

Anti-porcine CD3 immunotoxin

P. pastoris

$450/mg

RP3

Porcine IL3-Gly

P. pastoris

$200/mg

RP4

Human Annexin A2-Gly

P. pastoris

$200/mg

RP5

NHP IL3-Gly

P. pastoris

$200/mg

RP6

NHP IL3-NonGly

P. pastoris

$200/mg

RP7

Porcine IL3-NonGly

P. pastoris

$200/mg

RP8

Human C10orf54

P. pastoris

Inquiry

RP9

Mouse CD40L monomer

P. pastoris

RP10

Mouse CD40L dimer

P. pastoris

Inquiry

RP11

Mouse CD40L trimer

P. pastoris

RP12

Human IL2

P. pastoris

$22/million IU*

RP13

Mouse IL2

P. pastoris

$22/million IU*

RP14

Mouse Gi24

P. pastoris

Inquiry

RP15

pYD5 (yeast display plasmid vector)

$250/vial

RP16

Porcine CTLA4-Gly

P. pastoris

$450/mg

RP17

Human Annexin-A2-NonGly

P. pastoris

$200/mg

RP18

Porcine CTLA4-NonGly

P. pastoris

$450/mg

RP19

Human TNFR2

E.coli

RP20

Monkey TNFR2

E.coli

Inquiry

RP21

Human HB-EGF

P. pastoris

RP22

Porcine IL2-Gly

P. pastoris

$22/million IU*

RP23

Porcine IL2-NonGly

P. pastoris

$22/million IU*

RP24

pYD1 (yeast display plasmid vector)

$250 /vial

RP25

yeast Saccharomyces cerevisiae EBY100

$250 /vial

RP26

Porcine CD3 epsilon-gamma fusion protein

E.coli

Inquiry

New Recombinant Protein Development 

 P. Pastoris

$3500                      (see description)

Recombinant Protein Development Service Description

Amount

Development of recombinant yeast expression protocol and purification strategy including:

$2,500

Codon optimization for expression in pichia pastoris system

Synthesis of entire codon optimized gene

Sequence confirmation

Cloning the gene into a yeast expression vector

Transformation of yeast and selection of transformants

Development of the expression protocol using shaker flasks

Verification of the expression by SDS gel and Western blot analysis

NOTE: If the expression level is too low or the protein cannot be solubilized, project may be terminated at this stage and investigator will be charged $2500 for service performed

Development of the two step purification protocol (can be used in vivo)
For convenient purification, we will add a 6xHis Tag in the C-terminus

$1,000

Purified material from 2 L expression run using shaker flasks for functional testing

(No Charge)

Total

$3,500

The amount of material supplied depends on expression efficiency and yield after purification

Cost of additional protein to be determined based on expression level and yield