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Core Facilities

Tissue Microarray and Imaging (Pathology)

The sequencing of the human genome and the development of high-throughput gene expression technologies such as DNA microarrays have led to the discovery of hundreds or thousands of genes with a potential role in neoplastic transformation. Determining whether these genes are clinically relevant has been challenging due, in part, to limited availability of tissue samples necessary to validate these genes. The development of tissue microarray (TMA) technology has enabled the placement of hundreds of minute tissue samples on a single tissue block, thus conserving precious material. In addition, TMAs have increased throughput of in situ analysis because large numbers of tissue samples can be evaluated in a single experiment.

Access to this technology and these tissue-based resources is key to advancement of cancer research at DF/HCC. The mission of the Tissue Microarray and Imaging Core is to generate tissue microarrays by allowing placement of up to 480 0.6 mm diameter tissue samples into a single standard tissue block, allowing hundreds of tumors and samples to be evaluated on a single slide.

The Tissue Microarray and Imaging Core is dedicated to the construction and evaluation of high-quality tissue microarrays for cancer research. Core services include construction of custom tissue microarrays, array block sectioning, immunofluorescent staining, image acquisition & analysis, Aperio System services, high-throughput isolation of DNA and RNA from formalin-fixed paraffin-embedded tissue cores, as well as consultative services.

Leadership and Staff

Core Director: Sabina Signoretti, MD
Associate Pathologist: Scott Rodig, MD, PhD
Core Contact, TMA Construction: Chungdak Namgyal, 617-732-7723
Core Contact, Nucleic Acid Isolation: Teri Bowman, 617-525-7442

PUBLICATION ACKNOWLEDGEMENT: If research supported by this core facility results in publication, please acknowledge this support by including the following in your publication(s):

We thank Dana-Farber/Harvard Cancer Center in Boston, MA, for the use of the Tissue Microarray and Imaging Core, which provided __________ service. Dana-Farber/Harvard Cancer Center is supported in part by an NCI Cancer Center Support Grant # NIH 5 P30 CA06516.