DF/HCC Program Affiliation

Cancer Cell Biology

Research Abstract

We are interested in the mechanisms that direct development of the central nervous system (CNS) of vertebrates. Through lineage studies, we found that CNS progenitors generally are multipotent, with some exhibiting this even in their last cell division. As lineage alone does not appear to direct choice of cell fate of CNS cells, other signals are involved. We have chosen to focus on the signals in the vertebrate retina, a relatively simple and well-characterized area of the CNS. In vitro culture systems that mimic retinal development have been developed that allow study of the mitotic cycle, which is most likely intimately involved with cell fate decisions, as well as commitment and differentiation of retinal cells. These culture systems are being used to identify relevant extrinsic cues. In parallel, we are looking for intrinsic regulators. A recent approach is to use microarrays examine gene expression in single retinal progenitor cells and differentiating retinal cells. As well, we are cataloguing gene expression in all types of mature retinal neurons to enable the generation of markers for these cells and electrophysiological analysis in collaboration with other laboratories.

Publications

• Jadhav AP, Mason HA, Cepko CL.Notch 1 inhibits photoreceptor production in the developing mammalian retina.Development 2006 Mar;133(5):913-23.
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• Corbo JC, Cepko CL.A hybrid photoreceptor expressing both rod and cone genes in a mouse model of enhanced S-cone syndrome.PLoS Genet 2005 Aug;1(2):e11.
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• Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL.Genomic analysis of mouse retinal development.PLoS Biol 2004 Sep;2(9):E247.
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