DF/HCC Program Affiliation

Cancer Cell Biology

Research Abstract

Transcriptional regulation in response to environmental and developmental cues is mediated by the combinatorial and synergistic action of specific DNA-binding proteins on components of the general transcription machinery and on chromatin modifying activities. This laboratory combines genetic, molecular, and genomic approaches available in yeast and human cells to investigate a variety of issues. These include molecular mechanisms of transcriptional activation, relationship between chromatin structure of DNA and gene expression with particular emphasis epigenetic inheritance, and global transcriptional repression mechanisms mediated by corepressors that are recruited to specific genes. In addition, we are using genomic approaches to analyze transcriptional profiles and transcription factor occupancy during the transition of normal to transformed cells. Lastly, we are part of the ENCODE consortium to analyze genome-wide occupancy of transcription factors and chromatin-modifying activities in human cells.

Publications

• , Birney E, Stamatoyannopoulos JA, Dutta A, Guigó R, Gingeras TR, Margulies EH, Weng Z, Snyder M, Dermitzakis ET, Thurman RE, Kuehn MS, Taylor CM, Neph S, Koch CM, Asthana S, Malhotra A, Adzhubei I, Greenbaum JA, Andrews RM, Flicek P, Boyle PJ, Cao H, Car
  17571346
• Yang A, Zhu Z, Kapranov P, McKeon F, Church GM, Gingeras TR, Struhl K.Relationships between p63 binding, DNA sequence, transcription activity, and biological function in human cells.Mol Cell 2006 Nov 17;24(4):593-602.
  17188034
• Schwabish MA, Struhl K.Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II.Mol Cell 2006 May 5;22(3):415-22.
  16678113
• Cawley S, Bekiranov S, Ng HH, Kapranov P, Sekinger EA, Kampa D, Piccolboni A, Sementchenko V, Cheng J, Williams AJ, Wheeler R, Wong B, Drenkow J, Yamanaka M, Patel S, Brubaker S, Tammana H, Helt G, Struhl K, Gingeras TR.Unbiased mapping of transcription f
  14980218
• Kadosh D, Struhl K.Targeted recruitment of the Sin3-Rpd3 histone deacetylase complex generates a highly localized domain of repressed chromatin in vivo.Mol Cell Biol 1998 Sep;18(9):5121-7.
  9710596
• Moqtaderi Z, Bai Y, Poon D, Weil PA, Struhl K.TBP-associated factors are not generally required for transcriptional activation in yeast.Nature 1996 Sep 12;383(6596):188-91.
  8774887