Dr. Tenen's laboratory focuses on transcription factors and gene regulation, and their relationship to differentiation. The laboratory has characterized transcription factors which play a role in the differentiation of hematopoietic stem cells into different specific lineages, with particular focus on myeloid (granulocyte and monocyte) differentiation in normal and leukemic cells. The laboratory has isolated and characterized regulatory elements of master transcription factors expressed at different stages of differentiation, including stem and progenitor cells. Our studies have demonstrated a role for these transcription factors in the regulation of expression of important myeloid genes, and knockout studies demonstrated that they play a major role in hematopoietic development. In addition, we have identified mutations and specific abnormalities in expression of these factors in specific subtypes of myeloid leukemias. Part of the current efforts in the laboratory focus on understanding regulation, signal transduction pathways, and interacting partners of these factors, and their role in stem cells in normal hematopoiesis. In addition, a major effort in our laboratory is focused on further characterization of these factors in leukemogenesis, including developing drugs and other therapies specifically aimed at these factors. A related project in the lab is studying the role of transcription factors in normal lung differentiation, as well as in lung cancer. Other basic studies are investigating the role of long noncoding RNAs in gene regulation and chromatin structure. Techniques in the laboratory include analysis of regulation, function, and signaling of transcription factors, transgenic and knockout studies, and gene therapy applications.