Photo of David T. Breault,  MD, PhD

David T. Breault, MD, PhD

Boston Children's Hospital

Boston Children's Hospital
Phone: (617) 919-2863
Fax: (617) 730-0244


david.breault@childrens.harvard.edu

David T. Breault, MD, PhD

Boston Children's Hospital

EDUCATIONAL TITLES

  • Assistant Professor, Pediatrics, Harvard Medical School
  • Associate, Medicine, Boston Children's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

We are pursuing the hypothesis that slowly cycling and rapidly cycling intestinal stem cells exhibit distinctly different responses to tumor initiating signals. Preliminary results indicate that adenoma formation does not arise from slowly cycling (quiescent) stem cells. Ongoing work is focused on defining the mechanism underlying this tumor suppressor state, including analysis of gene expression and microRNA signatures.

Comparative analysis of rapidly and slowly cycling stem cells will provide unique insight into the mechanisms regulating the initial steps of cancer initiation.

Publications

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  • Gierut JJ, Lyons J, Shah MS, Genetti C, Breault DT, Haigis KM. Oncogenic K-Ras promotes proliferation in quiescent intestinal stem cells. 2015; 15:165-171. PubMed
  • Bennaceur K, Atwill M, Al Zhrany N, Hoffmann J, Keavney B, Breault D, Richardson G, von Zglinicki T, Saretzki G, Spyridopoulos I. Atorvastatin induces T cell proliferation by a telomerase reverse transcriptase (TERT) mediated mechanism. Atherosclerosis 2014; 236:312-20. PubMed
  • Freedman BD, Kempna PB, Carlone DL, Shah MS, Guagliardo NA, Barrett PQ, Gomez-Sanchez CE, Majzoub JA, Breault DT. Adrenocortical zonation results from lineage conversion of differentiated zona glomerulosa cells. Dev Cell 2013; 26:666-73. PubMed
  • Meeson A, Fuller A, Breault DT, Owens WA, Richardson GD. Optimised Protocols for the Identification of the Murine Cardiac Side Population. Stem Cell Rev 2013. PubMed
  • Richardson GD, Breault D, Horrocks G, Cormack S, Hole N, Owens WA. Telomerase expression in the mammalian heart. FASEB J. 2012. PubMed
  • Kodama Y, Noda A, Booth C, Breault D, Suda T, Hendry J, Shinohara T, RĂ¼be C, Nishimura EK, Mitani H, Nakamura N, Niwa O. International workshop: radiation effects on mutation in somatic and germline stem cells. Int J Radiat Biol 2012; 88:501-6. PubMed
  • Carlone DL, Breault DT. Tales from the crypt: the expanding role of slow cycling intestinal stem cells. Cell Stem Cell 2012; 10:2-4. PubMed
  • Song J, Czerniak S, Wang T, Ying W, Carlone DL, Breault DT, Humphreys BD. Characterization and fate of telomerase-expressing epithelia during kidney repair. J Am Soc Nephrol 2011; 22:2256-65. PubMed
  • Carlone DL, Breault DT. Slowly cycling versus rapidly cycling intestinal stem cells: Distinct roles or redundancy. Cell Cycle 2011; 10:723-4. PubMed
  • Montgomery RK, Carlone DL, Richmond CA, Farilla L, Kranendonk ME, Henderson DE, Baffour-Awuah NY, Ambruzs DM, Fogli LK, Algra S, Breault DT. Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling intestinal stem cells. Proc Natl Acad Sci U S A 2011; 108:179-84. PubMed
  • Richmond CA, Breault DT. Regulation of gene expression in the intestinal epithelium. 2010; 96:207-29. PubMed
  • Breault DT,Min IM,Carlone DL,Farilla LG,Ambruzs DM,Henderson DE,Algra S,Montgomery RK,Wagers AJ,Hole N. Generation of mTert-GFP mice as a model to identify and study tissue progenitor cells. Proc Natl Acad Sci U S A 2008; 105:10420-5. PubMed
  • Stadtfeld M, Maherali N, Breault DT, Hochedlinger K. Defining molecular cornerstones during fibroblast to iPS cell reprogramming in mouse. Cell Stem Cell 2008; 2:230-40. PubMed
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