Photo of Donald E. Ingber,  MD, PhD

Donald E. Ingber, MD, PhD

Boston Children's Hospital

Boston Children's Hospital
Phone: (617) 432-7044
Fax: (617) 432-7828


don.ingber@wyss.harvard.edu

Donald E. Ingber, MD, PhD

Boston Children's Hospital

EDUCATIONAL TITLES

  • Judah Folkman Professor of Vascular Biology, Pathology, Harvard Medical School
  • Research Associate, Pathology, Brigham And Women's Hospital
  • Research Associate, Vascular Biology Program, Boston Children's Hospital
  • Director, Executive Team, Wyss Institute for Biologically Inspired Engineering at Harvard Univ

Research Abstract

Dr. Ingber's research group focuses on the importance of extracellular matrix (ECM), integrins, and cytoskeletal structure for control of angiogenesis. They combine methods from diverse fields, including molecular cell biology, mechanical engineering, chemistry, and materials science, to address fundamental questions relating to the mechanism of capillary cell growth, differentiation, migration and apoptosis. Ingber was one of the first investigators to show that chemical modulators of ECM turnover can inhibit angiogenesis and induce capillary regression. His discovery also led to the development of the angiogenesisinhibitor, TNP-470, that is currently in human clinical trials.

Publications

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  • Mammoto T, Mammoto A, Jiang A, Jiang E, Hashmi B, Ingber DE. Mesenchymal condensation-dependent accumulation of collagen VI stabilizes organ-specific cell fates during embryonic tooth formation. Dev Dyn 2015. PubMed
  • Lucio AA, Ingber DE, Campàs O. Generation of biocompatible droplets for in vivo and in vitro measurement of cell-generated mechanical stresses. Methods Cell Biol. 2015; 125:373-90. PubMed
  • Yang Z, Liu Q, Mannix RJ, Xu X, Li H, Ma Z, Ingber DE, Allen PD, Wang Y. Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability. Stem Cells 2014. PubMed
  • Kanapathipillai M, Brock A, Ingber DE. Nanoparticle targeting of anti-cancer drugs that alter intracellular signaling or influence the tumor microenvironment. Adv Drug Deliv Rev 2014. PubMed
  • German AE, Mammoto T, Jiang E, Ingber DE, Mammoto A. Paxillin controls endothelial cell migration and tumor angiogenesis by altering neuropilin 2 expression. J Cell Sci 2014; 127:1672-83. PubMed
  • Ingber DE, Wang N, Stamenović D. Tensegrity, cellular biophysics, and the mechanics of living systems. Rep Prog Phys 2014; 77:046603. PubMed
  • Polacheck WJ, German AE, Mammoto A, Ingber DE, Kamm RD. Mechanotransduction of fluid stresses governs 3D cell migration. Proc Natl Acad Sci U S A 2014; 111:2447-52. PubMed
  • Campàs O, Mammoto T, Hasso S, Sperling RA, O'Connell D, Bischof AG, Maas R, Weitz DA, Mahadevan L, Ingber DE. Quantifying cell-generated mechanical forces within living embryonic tissues. Nat Methods 2014; 11:183-9. PubMed
  • Brock A, Krause S, Li H, Kowalski M, Goldberg MS, Collins JJ, Ingber DE. Silencing HoxA1 by intraductal injection of siRNA lipidoid nanoparticles prevents mammary tumor progression in mice. Sci Transl Med 2014; 6:217ra2. PubMed
  • Dolatshahi-Pirouz A, Nikkhah M, Gaharwar AK, Hashmi B, Guermani E, Aliabadi H, Camci-Unal G, Ferrante T, Foss M, Ingber DE, Khademhosseini A. A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells. Sci Rep 2014; 4:3896. PubMed
  • Huh D, Kim HJ, Fraser JP, Shea DE, Khan M, Bahinski A, Hamilton GA, Ingber DE. Microfabrication of human organs-on-chips. Nat Protoc 2013; 8:2135-57. PubMed
  • Deiss F, Mazzeo A, Hong E, Ingber DE, Derda R, Whitesides GM. Platform for high-throughput testing of the effect of soluble compounds on 3D cell cultures. Anal Chem 2013; 85:8085-94. PubMed
  • Kim HJ, Ingber DE. Gut-on-a-Chip microenvironment induces human intestinal cells to undergo villus differentiation. Integr Biol (Camb) 2013; 5:1130-40. PubMed
  • Panigrahy D, Kalish BT, Huang S, Bielenberg DR, Le HD, Yang J, Edin ML, Lee CR, Benny O, Mudge DK, Butterfield CE, Mammoto A, Mammoto T, Inceoglu B, Jenkins RL, Simpson MA, Akino T, Lih FB, Tomer KB, Ingber DE, Hammock BD, Falck JR, Manthati VL, Kaipainen A, D'Amore PA, Puder M, Zeldin DC, Kieran MW. Epoxyeicosanoids promote organ and tissue regeneration. Proc Natl Acad Sci U S A 2013; 110:13528-33. PubMed
  • Bischof AG, Yüksel D, Mammoto T, Mammoto A, Krause S, Ingber DE. Breast cancer normalization induced by embryonic mesenchyme is mediated by extracellular matrix biglycan. Integr Biol (Camb) 2013; 5:1045-56. PubMed
  • Gee EP, Yüksel D, Stultz CM, Ingber DE. SLLISWD sequence in the 10FNIII domain initiates fibronectin fibrillogenesis. J Biol Chem 2013; 288:21329-40. PubMed
  • Mammoto A, Mammoto T, Kanapathipillai M, Wing Yung C, Jiang E, Jiang A, Lofgren K, Gee EP, Ingber DE. Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics. Nat Commun 2013; 4:1759. PubMed
  • Werfel J, Krause S, Bischof AG, Mannix RJ, Tobin H, Bar-Yam Y, Bellin RM, Ingber DE. How changes in extracellular matrix mechanics and gene expression variability might combine to drive cancer progression. PLoS ONE 2013; 8:e76122. PubMed
  • Mammoto T, Mammoto A, Ingber DE. Mechanobiology and developmental control. Annu Rev Cell Dev Biol 2013; 29:27-61. PubMed
  • Krause S, Brock A, Ingber DE. Intraductal injection for localized drug delivery to the mouse mammary gland. 2013. PubMed
  • Mammoto A, Mammoto T, Ingber DE. Mechanosensitive mechanisms in transcriptional regulation. J Cell Sci 2012; 125:3061-73. PubMed
  • Kanapathipillai M, Mammoto A, Mammoto T, Kang JH, Jiang E, Ghosh K, Korin N, Gibbs A, Mannix R, Ingber DE. Inhibition of mammary tumor growth using lysyl oxidase-targeting nanoparticles to modify extracellular matrix. Nano Lett 2012; 12:3213-7. PubMed
  • Xu Y, Bismar TA, Su J, Xu B, Kristiansen G, Varga Z, Teng L, Ingber DE, Mammoto A, Kumar R, Alaoui-Jamali MA. Filamin A regulates focal adhesion disassembly and suppresses breast cancer cell migration and invasion. J Exp Med 2010; 207:2421-37. PubMed
  • Mammoto T, Ingber DE. Mechanical control of tissue and organ development. Development 2010; 137:1407-20. PubMed
  • Huang S, Ingber DE. The structural and mechanical complexity of cell-growth control. Nat Cell Biol 1999; 1:E131-8. PubMed
  • Dike LE, Chen CS, Mrksich M, Tien J, Whitesides GM, Ingber DE. Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 1999; 35:441-8. PubMed
  • Huang S, Chen CS, Ingber DE. Control of cyclin D1, p27(Kip1), and cell cycle progression in human capillary endothelial cells by cell shape and cytoskeletal tension. Mol Biol Cell 1998; 9:3179-93. PubMed
  • Chicurel ME, Singer RH, Meyer CJ, Ingber DE. Integrin binding and mechanical tension induce movement of mRNA and ribosomes to focal adhesions. Nature 1998; 392:730-3. PubMed
  • Chicurel ME, Chen CS, Ingber DE. Cellular control lies in the balance of forces. Curr Opin Cell Biol 1998; 10:232-9. PubMed
  • Chen CS, Mrksich M, Huang S, Whitesides GM, Ingber DE. Geometric control of cell life and death. Science 1997; 276:1425-8. PubMed
  • Ezzell RM, Goldmann WH, Wang N, Parasharama N, Ingber DE. Vinculin promotes cell spreading by mechanically coupling integrins to the cytoskeleton. Exp Cell Res 1997; 231:14-26. PubMed
  • Maniotis AJ, Chen CS, Ingber DE. Demonstration of mechanical connections between integrins, cytoskeletal filaments, and nucleoplasm that stabilize nuclear structure. Proc Natl Acad Sci U S A 1997; 94:849-54. PubMed
  • Dike LE, Ingber DE. Integrin-dependent induction of early growth response genes in capillary endothelial cells. J Cell Sci 1996; 109 ( Pt 12):2855-63. PubMed
  • Mrksich M, Chen CS, Xia Y, Dike LE, Ingber DE, Whitesides GM. Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold. Proc Natl Acad Sci U S A 1996; 93:10775-8. PubMed
  • Stamenovic D, Fredberg JJ, Wang N, Butler JP, Ingber DE. A microstructural approach to cytoskeletal mechanics based on tensegrity. J Theor Biol 1996; 181:125-36. PubMed
  • McNamee HP, Liley HG, Ingber DE. Integrin-dependent control of inositol lipid synthesis in vascular endothelial cells and smooth muscle cells. Exp Cell Res 1996; 224:116-22. PubMed
  • Yoshida M, Westlin WF, Wang N, Ingber DE, Rosenzweig A, Resnick N, Gimbrone MA. Leukocyte adhesion to vascular endothelium induces E-selectin linkage to the actin cytoskeleton. J Cell Biol 1996; 133:445-55. PubMed
  • Ingber DE, Prusty D, Sun Z, Betensky H, Wang N. Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis. J Biomech 1995; 28:1471-84. PubMed
  • Mooney DJ, Langer R, Ingber DE. Cytoskeletal filament assembly and the control of cell spreading and function by extracellular matrix. J Cell Sci 1995; 108 ( Pt 6):2311-20. PubMed
  • Wang N, Ingber DE. Control of cytoskeletal mechanics by extracellular matrix, cell shape, and mechanical tension. Biophys J 1994; 66:2181-9. PubMed
  • Singhvi R, Kumar A, Lopez GP, Stephanopoulos GN, Wang DI, Whitesides GM, Ingber DE. Engineering cell shape and function. Science 1994; 264:696-8. PubMed
  • Schwartz MA, Ingber DE. Integrating with integrins. Mol Biol Cell 1994; 5:389-93. PubMed
  • Ingber DE. The riddle of morphogenesis: a question of solution chemistry or molecular cell engineering? Cell 1993; 75:1249-52. PubMed
  • Wang N, Butler JP, Ingber DE. Mechanotransduction across the cell surface and through the cytoskeleton. Science 1993; 260:1124-7. PubMed
  • McNamee HP, Ingber DE, Schwartz MA. Adhesion to fibronectin stimulates inositol lipid synthesis and enhances PDGF-induced inositol lipid breakdown. J Cell Biol 1993; 121:673-8. PubMed
  • Sims JR, Karp S, Ingber DE. Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape. J Cell Sci 1992; 103 ( Pt 4):1215-22. PubMed
  • Schwartz MA, Lechene C, Ingber DE. Insoluble fibronectin activates the Na/H antiporter by clustering and immobilizing integrin alpha 5 beta 1, independent of cell shape. Proc Natl Acad Sci U S A 1991; 88:7849-53. PubMed
  • Ingber DE. Fibronectin controls capillary endothelial cell growth by modulating cell shape. Proc Natl Acad Sci U S A 1990; 87:3579-83. PubMed
  • Ingber DE, Prusty D, Frangioni JV, Cragoe EJ, Lechene C, Schwartz MA. Control of intracellular pH and growth by fibronectin in capillary endothelial cells. J Cell Biol 1990; 110:1803-11. PubMed
  • Ingber DE, Folkman J. How does extracellular matrix control capillary morphogenesis? Cell 1989; 58:803-5. PubMed
  • Ingber DE, Folkman J. Mechanochemical switching between growth and differentiation during fibroblast growth factor-stimulated angiogenesis in vitro: role of extracellular matrix. J Cell Biol 1989; 109:317-30. PubMed
  • Ingber DE, Madri JA, Folkman J. Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion. In Vitro Cell Dev Biol 1987; 23:387-94. PubMed
  • Ingber DE, Madri JA, Folkman J. A possible mechanism for inhibition of angiogenesis by angiostatic steroids: induction of capillary basement membrane dissolution. Endocrinology 1986; 119:1768-75. PubMed
  • Ingber DE, Madri JA, Jamieson JD. Basement membrane as a spatial organizer of polarized epithelia. Exogenous basement membrane reorients pancreatic epithelial tumor cells in vitro. Am J Pathol 1985; 122:129-39. PubMed
  • Ingber DE, Madri JA, Jamieson JD. Role of basal lamina in neoplastic disorganization of tissue architecture. Proc Natl Acad Sci U S A 1981; 78:3901-5. PubMed
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