Photo of James A. DeCaprio,  MD

James A. DeCaprio, MD

Dana-Farber Cancer Institute

Dana-Farber Cancer Institute
Phone: (617) 632-3825
Fax: (617) 582-8601


james_decaprio@dfci.harvard.edu

James A. DeCaprio, MD

Dana-Farber Cancer Institute

EDUCATIONAL TITLES

  • Professor, Medicine, Harvard Medical School
  • Active Staff and Physician, Medicine, Brigham And Women's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

The role of Merkel cell polyomavirus in Merkel cell carcinoma

Merkel cell carcinoma is a skin cancer with a high rate of mortality. Factors that increase the risk for developing MCC include excessive exposure to sunlight, advanced age and an immunocompromised state. Recognition that immunodeficiency increased the risk for developing Merkel cell carcinoma prompted a focused search for pathogens and the discovery of Merkel cell polyomavirus. In at least 80% of all Merkel cell carcinomas, the Merkel cell polyomavirus small T antigen (ST) is intact, while the large T antigen (LT) is truncated. Given our laboratory’s experience in studying polyomaviruses especially SV40, we initiated studies of Merkel cell polyomavirus LT and ST. We generated monoclonal antibodies specific for LT and ST that had improved specificity and sensitivity for immunohistochemical detection of MCPyV in MCC tumor specimens that indicated that most cases of Merkel cell carcinoma express the viral T antigens. We generated a mouse knock-in model capable of tissue specific expression of the Merkel virus T antigens and demonstrated their oncogenic potential in vivo. We continue to use advanced proteomics, genomics and bioinformatic approaches to identify the tumorigenic properties of the Merkel cell polyomavirus T antigens in patient samples, mouse models and cell lines.

The DREAM (DP, RB-related, E2F and MuvB) complex

Our laboratory demonstrated that the Retinoblastoma-related proteins p130 and p107 provide tumor suppressor activities. To determine how p130 contributed to growth suppression, we performed a large-scale immunoprecipitation followed by mass spectrometric identification of associated proteins. We identified an 8-protein complex that we termed DREAM based on its similarity to complexes previously identified by genetic and biochemical studies in C. elegans and D. melanogaster. We determined that the mammalian DREAM complex bound specifically to the promoters of all cell cycle regulated genes and repressed their expression during cellular quiescence. We determined that the DYRK1A kinase was required for assembly of the DREAM complex during quiescence. We also found that the DREAM complex underwent a metamorphosis during cell cycle progression with the MuvB component being released from p130, E2F4 and DP1 during the G1 phase of the cell cycle and subsequently binding to B-MYB (MYBL2) and FOXM1 to specifically activate expression of several hundred genes required for progression during G2 and M phase. Our work demonstrated that the DREAM complex serves as a master coordinator of cell cycle gene expression. We continue to study the DREAM complex to understand how cell cycle dependent gene expression is controlled during the major transition phases of the cell cycle including G0, G1/S and G2/M.

Identification of human disease genes by study of Viral protein-Host cell protein interactions

Our laboratory identified CUL7, CUL9, FBXW8, GLMN and FAM111A as specific interacting proteins with SV40 LT and generated knockout mouse models for Cul7, Fbxw8, Cul9 and Glmn genes. We demonstrated that knockout of Cul7 or Fbxw8 led to severe growth retardation that was recognized by an independent genetic study that determined that homozygous mutations in CUL7 were responsible for the human 3M short stature syndrome. We demonstrated that GLMN binds directly to RBX1 and inhibits the ubiquitin ligase activity of cullin RING ligases and revealed the molecular basis for Glomuvenous malformation, a human hereditary vascular malformation syndrome. Most recently, we identified FAM111A as an SV40 host range restriction factor. Remarkably, mutations in FAM111A have recently been described in the short stature Kenny-Caffey and osteocraniostenosis syndromes. These studies of viral-host cell protein interactions have led to the molecular characterization of genes that are mutated in a variety of human diseases and suggest that LT targets cellular and organismal growth-promoting activities. Our laboratory focuses on deciphering the molecular functions of these viral-targeted host genes. We have performed a large-scale viral interactome and transcriptome screen that has fueled several new research projects and are actively pursuing new viral protein interactions with other disease related genes in addition to FAM111A and GLMN.

Publications

Powered by Harvard Catalyst
  • Fischer M, DeCaprio JA. Does Arabidopsis thaliana DREAM of cell cycle control? EMBO J 2015. PubMed
  • Spurgeon ME, Cheng J, Bronson RT, Lambert PF, DeCaprio JA. Tumorigenic activity of merkel cell polyomavirus T antigens expressed in the stratified epithelium of mice. Cancer Res 2015; 75:1068-79. PubMed
  • Berrios C, Jung J, Primi B, Wang M, Pedamallu C, Duke F, Marcelus C, Cheng J, Garcea RL, Meyerson M, DeCaprio JA, . Malawi polyomavirus is a prevalent human virus that interacts with known tumor suppressors. J Virol 2014. PubMed
  • Smith JA, Haberstroh FS, White EA, Livingston DM, DeCaprio JA, Howley PM. SMCX and components of the TIP60 complex contribute to E2 regulation of the HPV E6/E7 promoter. Virology 2014; 468-470C:311-321. PubMed
  • DeCaprio JA, Duensing A. The DREAM complex in antitumor activity of imatinib mesylate in gastrointestinal stromal tumors. Curr Opin Oncol 2014; 26:415-21. PubMed
  • Naetar N, Soundarapandian V, Litovchick L, Goguen KL, Sablina AA, Bowman-Colin C, Sicinski P, Hahn WC, DeCaprio JA, Livingston DM. PP2A-Mediated Regulation of Ras Signaling in G2 Is Essential for Stable Quiescence and Normal G1 Length. Mol Cell 2014. PubMed
  • Forristal C, Henley SA, Macdonald JI, Bush JR, Ort C, Passos DT, Talluri S, Ishak CA, Thwaites M, Norley CJ, Litovchick L, Decaprio JA, Dimattia G, Holdsworth DW, Beier F, Dick FA. Loss of the mammalian DREAM complex deregulates chondrocyte proliferation. Mol Cell Biol 2014. PubMed
  • Orzalli MH, Conwell SE, Berrios C, DeCaprio JA, Knipe DM. Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and transfected DNA. Proc Natl Acad Sci U S A 2013; 110:E4492-501. PubMed
  • Ivanova T, Alves-Rodrigues I, Gómez-Escoda B, Dutta C, DeCaprio JA, Rhind N, Hidalgo E, Ayté J. The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor. Mol Biol Cell 2013; 24:3350-7. PubMed
  • Boichuk S, Parry JA, Makielski KR, Litovchick L, Baron JL, Zewe JP, Wozniak A, Mehalek KR, Korzeniewski N, Seneviratne DS, Schöffski P, Debiec-Rychter M, DeCaprio JA, Duensing A. The DREAM complex mediates GIST cell quiescence and is a novel therapeutic target to enhance imatinib-induced apoptosis. Cancer Res 2013; 73:5120-9. PubMed
  • Sadasivam S, DeCaprio JA. The DREAM complex: master coordinator of cell cycle-dependent gene expression. Nat Rev Cancer 2013; 13:585-95. PubMed
  • Decaprio JA, Garcea RL. A cornucopia of human polyomaviruses. Nat Rev Microbiol 2013; 11:264-76. PubMed
  • Cheng J, Rozenblatt-Rosen O, Paulson KG, Nghiem P, Decaprio JA. Merkel Cell Polyomavirus Large T Antigen has Growth Promoting and Inhibitory Activities. J Virol 2013. PubMed
  • Rodig SJ, Cheng J, Wardzala J, DoRosario A, Scanlon JJ, Laga AC, Martinez-Fernandez A, Barletta JA, Bellizzi AM, Sadasivam S, Holloway DT, Cooper DJ, Kupper TS, Wang LC, DeCaprio JA. Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus. J Clin Invest 2012; 122:4645-53. PubMed
  • Fine DA, Rozenblatt-Rosen O, Padi M, Korkhin A, James RL, Adelmant G, Yoon R, Guo L, Berrios C, Zhang Y, Calderwood MA, Velmurgan S, Cheng J, Marto JA, Hill DE, Cusick ME, Vidal M, Florens L, Washburn MP, Litovchick L, Decaprio JA. Identification of FAM111A as an SV40 Host Range Restriction and Adenovirus Helper Factor. PLoS Pathog. 2012; 8:e1002949. PubMed
  • Duda DM, Olszewski JL, Tron AE, Hammel M, Lambert LJ, Waddell MB, Mittag T, DeCaprio JA, Schulman BA. Structure of a glomulin-RBX1-CUL1 complex: inhibition of a RING E3 ligase through masking of its E2-binding surface. Mol Cell 2012; 47:371-82. PubMed
  • Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, Barabási AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Münger K, Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M. Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Nature 2012; 487:491-5. PubMed
  • Min SH, Lau AW, Lee TH, Inuzuka H, Wei S, Huang P, Shaik S, Lee DY, Finn G, Balastik M, Chen CH, Luo M, Tron AE, Decaprio JA, Zhou XZ, Wei W, Lu KP. Negative regulation of the stability and tumor suppressor function of Fbw7 by the Pin1 prolyl isomerase. Mol Cell 2012; 46:771-83. PubMed
  • Tron AE, Arai T, Duda DM, Kuwabara H, Olszewski JL, Fujiwara Y, Bahamon BN, Signoretti S, Schulman BA, Decaprio JA. The Glomuvenous Malformation Protein Glomulin Binds Rbx1 and Regulates Cullin RING Ligase-Mediated Turnover of Fbw7. Mol Cell 2012. PubMed
  • Sadasivam S, Duan S, Decaprio JA. The MuvB complex sequentially recruits B-Myb and FoxM1 to promote mitotic gene expression. Genes Dev 2012; 26:474-89. PubMed
  • Müller GA, Quaas M, Schümann M, Krause E, Padi M, Fischer M, Litovchick L, Decaprio JA, Engeland K. The CHR promoter element controls cell cycle-dependent gene transcription and binds the DREAM and MMB complexes. Nucleic Acids Res 2012; 40:1561-78. PubMed
  • Gulbahce N, Yan H, Dricot A, Padi M, Byrdsong D, Franchi R, Lee DS, Rozenblatt-Rosen O, Mar JC, Calderwood MA, Baldwin A, Zhao B, Santhanam B, Braun P, Simonis N, Huh KW, Hellner K, Grace M, Chen A, Rubio R, Marto JA, Christakis NA, Kieff E, Roth FP, Roecklein-Canfield J, Decaprio JA, Cusick ME, Quackenbush J, Hill DE, Münger K, Vidal M, Barabási AL. Viral perturbations of host networks reflect disease etiology. PLoS Comput. Biol. 2012; 8:e1002531. PubMed
  • Litovchick L, Florens LA, Swanson SK, Washburn MP, DeCaprio JA. DYRK1A protein kinase promotes quiescence and senescence through DREAM complex assembly. Genes Dev 2011; 25:801-13. PubMed
  • Tschöp K, Conery AR, Litovchick L, Decaprio JA, Settleman J, Harlow E, Dyson N. A kinase shRNA screen links LATS2 and the pRB tumor suppressor. Genes Dev 2011; 25:814-30. PubMed
  • Gao D, Wan L, Inuzuka H, Berg AH, Tseng A, Zhai B, Shaik S, Bennett E, Tron AE, Gasser JA, Lau A, Gygi SP, Harper JW, DeCaprio JA, Toker A, Wei W. Rictor forms a complex with Cullin-1 to promote SGK1 ubiquitination and destruction. Mol Cell 2010; 39:797-808. PubMed
  • Cheng J, DeCaprio JA, Fluck MM, Schaffhausen BS. Cellular transformation by Simian Virus 40 and Murine Polyoma Virus T antigens. Semin Cancer Biol 2009; 19:218-28. PubMed
  • DeCaprio JA. Does detection of Merkel cell polyomavirus in Merkel cell carcinoma provide prognostic information? Journal of the National Cancer Institute 2009; 101:905-7. PubMed
  • Harsha HC,Kandasamy K,Ranganathan P,Rani S,Ramabadran S,Gollapudi S,Balakrishnan L,Dwivedi SB,Telikicherla D,Selvan LD,Goel R,Mathivanan S,Marimuthu A,Kashyap M,Vizza RF,Mayer RJ,Decaprio JA,Srivastava S,Hanash SM,Hruban RH,Pandey A. A compendium of potential biomarkers of pancreatic cancer. PLoS Med 2009; 6:e1000046. PubMed
  • DeCaprio JA. How the Rb tumor suppressor structure and function was revealed by the study of Adenovirus and SV40. Virology 2009; 384:274-84. PubMed
  • Kundu ST,Gosavi P,Khapare N,Patel R,Hosing AS,Maru GB,Ingle A,Decaprio JA,Dalal SN. Plakophilin3 downregulation leads to a decrease in cell adhesion and promotes metastasis. Int J Cancer 2008; 123:2303-14. PubMed
  • Borger DR, Gavrilescu LC, Bucur MC, Ivan M, Decaprio JA. AMP-activated protein kinase is essential for survival in chronic hypoxia. Biochem Biophys Res Commun 2008; 370:230-4. PubMed
  • Garneski KM,DeCaprio JA,Nghiem P. Does a new polyomavirus contribute to Merkel cell carcinoma? Genome Biol 2008; 9:228. PubMed
  • Tsutsumi T, Kuwabara H, Arai T, Xiao Y, Decaprio JA. Disruption of the Fbxw8 gene results in pre- and postnatal growth retardation in mice. Mol Cell Biol 2007; 28:743-51. PubMed
  • Sablina AA, Chen W, Arroyo JD, Corral L, Hector M, Bulmer SE, Decaprio JA, Hahn WC. The Tumor Suppressor PP2A Abeta Regulates the RalA GTPase. Cell 2007; 129:969-982. PubMed
  • Litovchick L, Sadasivam S, Florens L, Zhu X, Swanson SK, Velmurugan S, Chen R, Washburn MP, Liu XS, Decaprio JA. Evolutionarily Conserved Multisubunit RBL2/p130 and E2F4 Protein Complex Represses Human Cell Cycle-Dependent Genes in Quiescence. Mol Cell 2007; 26:539-51. PubMed
  • Skaar JR, Florens L, Tsutsumi T, Arai T, Tron A, Swanson SK, Washburn MP, DeCaprio JA. PARC and CUL7 form atypical cullin RING ligase complexes. Cancer Res 2007; 67:2006-14. PubMed
  • Kasper JS, Arai T, Decaprio JA. A novel p53-binding domain in CUL7. Biochem Biophys Res Commun 2006; 348:132-8. PubMed
  • Poulin DL, DeCaprio JA. Is there a role for SV40 in human cancer? J Clin Oncol 2006; 24:4356-65. PubMed
  • Poulin DL, DeCaprio JA. The carboxyl-terminal domain of large T antigen rescues SV40 host range activity in trans independent of acetylation. Virology 2006; 349:212-21. PubMed
  • Borger DR, DeCaprio JA. Targeting of p300/CREB binding protein coactivators by simian virus 40 is mediated through p53. J Virol 2006; 80:4292-303. PubMed
  • Kasper JS, Kuwabara H, Arai T, Ali SH, DeCaprio JA. Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation. J Virol 2005; 79:11685-92. PubMed
  • Skaar JR, Arai T, DeCaprio JA. Dimerization of CUL7 and PARC is not required for all CUL7 functions and mouse development. Mol Cell Biol 2005; 25:5579-89. PubMed
  • Litovchick L, Chestukhin A, DeCaprio JA. Glycogen synthase kinase 3 phosphorylates RBL2/p130 during quiescence. Mol Cell Biol 2004; 24:8970-80. PubMed
  • Poulin DL, Kung AL, DeCaprio JA. p53 targets simian virus 40 large T antigen for acetylation by CBP. J Virol 2004; 78:8245-53. PubMed
  • Buchmann AM, Skaar JR, DeCaprio JA. Activation of a DNA damage checkpoint response in a TAF1-defective cell line. Mol Cell Biol 2004; 24:5332-9. PubMed
  • Dalal SN, Yaffe MB, DeCaprio JA. 14-3-3 family members act coordinately to regulate mitotic progression. Cell Cycle 2004; 3:672-7. PubMed
  • Ali SH, Kasper JS, Arai T, DeCaprio JA. Cul7/p185/p193 binding to simian virus 40 large T antigen has a role in cellular transformation. J Virol 2004; 78:2749-57. PubMed
  • Qi H, Gervais ML, Li W, DeCaprio JA, Challis JR, Ohh M. Molecular cloning and characterization of the von Hippel-Lindau-like protein. Mol Cancer Res 2004; 2:43-52. PubMed
  • Towbin H, Bair KW, DeCaprio JA, Eck MJ, Kim S, Kinder FR, Morollo A, Mueller DR, Schindler P, Song HK, van Oostrum J, Versace RW, Voshol H, Wood J, Zabludoff S, Phillips PE. Proteomics-based target identification: bengamides as a new class of methionine aminopeptidase inhibitors. J Biol Chem 2003; 278:52964-71. PubMed
  • Lin JY, DeCaprio JA. SV40 large T antigen promotes dephosphorylation of p130. J Biol Chem 2003; 278:46482-7. PubMed
  • Arai T, Kasper JS, Skaar JR, Ali SH, Takahashi C, DeCaprio JA. Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis. Proc Natl Acad Sci U S A 2003; 100:9855-60. PubMed
  • Masutomi K, Yu EY, Khurts S, Ben-Porath I, Currier JL, Metz GB, Brooks MW, Kaneko S, Murakami S, DeCaprio JA, Weinberg RA, Stewart SA, Hahn WC. Telomerase maintains telomere structure in normal human cells. Cell 2003; 114:241-53. PubMed
  • Sanhes L, Tang R, Delmer A, DeCaprio JA, Ajchenbaum-Cymbalista F. Fludarabine-induced apoptosis of B chronic lymphocytic leukemia cells includes early cleavage of p27kip1 by caspases. Leukemia 2003; 17:1104-11. PubMed
  • Li D, Zhao R, Lilyestrom W, Gai D, Zhang R, DeCaprio JA, Fanning E, Jochimiak A, Szakonyi G, Chen XS. Structure of the replicative helicase of the oncoprotein SV40 large tumour antigen. Nature 2003; 423:512-8. PubMed
  • Chestukhin A, Pfeffer C, Milligan S, DeCaprio JA, Pellman D. Processing, localization, and requirement of human separase for normal anaphase progression. Proc Natl Acad Sci U S A 2003; 100:4574-9. PubMed
  • Chestukhin A, DeCaprio JA. Western blot screening for monoclonal antibodies against human separase. J Immunol Methods 2003; 274:105-13. PubMed
  • Baksh S, Widlund HR, Frazer-Abel AA, Du J, Fosmire S, Fisher DE, DeCaprio JA, Modiano JF, Burakoff SJ. NFATc2-mediated repression of cyclin-dependent kinase 4 expression. Mol Cell 2002; 10:1071-81. PubMed
  • Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, DeCaprio JA, Weinberg RA. Enumeration of the simian virus 40 early region elements necessary for human cell transformation. Mol Cell Biol 2002; 22:2111-23. PubMed
  • Brunet A, Kanai F, Stehn J, Xu J, Sarbassova D, Frangioni JV, Dalal SN, DeCaprio JA, Greenberg ME, Yaffe MB. 14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport. J Cell Biol 2002; 156:817-28. PubMed
  • Chestukhin A, Litovchick L, Rudich K, DeCaprio JA. Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism. Mol Cell Biol 2002; 22:453-68. PubMed
  • Ayt. Feedback regulation of the MBF transcription factor by cyclin Cig2. Nat Cell Biol 2002; 3:1043-50. PubMed
  • Hall C, Nelson DM, Ye X, Baker K, DeCaprio JA, Seeholzer S, Lipinski M, Adams PD. HIRA, the human homologue of yeast Hir1p and Hir2p, is a novel cyclin-cdk2 substrate whose expression blocks S-phase progression. Mol Cell Biol 2001; 21:1854-65. PubMed
  • Ali SH, DeCaprio JA. Cellular transformation by SV40 large T antigen: interaction with host proteins. Semin Cancer Biol 2001; 11:15-23. PubMed
  • Zalvide J, DeCaprio JA, Stubdal H. Binding of SV40 large T antigen to the retinoblastoma susceptibility gene product and related proteins. Methods Mol Biol 2001; 165:213-8. PubMed
  • Miyake S, Sellers WR, Safran M, Li X, Zhao W, Grossman SR, Gan J, DeCaprio JA, Adams PD, Kaelin WG. Cells degrade a novel inhibitor of differentiation with E1A-like properties upon exiting the cell cycle. Mol Cell Biol 2000; 20:8889-902. PubMed
  • Canhoto AJ, Chestukhin A, Litovchick L, DeCaprio JA. Phosphorylation of the retinoblastoma-related protein p130 in growth-arrested cells. Oncogene 2000; 19:5116-22. PubMed
  • Chao HH, Buchmann AM, DeCaprio JA. Loss of p19(ARF) eliminates the requirement for the pRB-binding motif in simian virus 40 large T antigen-mediated transformation. Mol Cell Biol 2000; 20:7624-33. PubMed
  • Vairo G, Soos TJ, Upton TM, Zalvide J, DeCaprio JA, Ewen ME, Koff A, Adams JM. Bcl-2 retards cell cycle entry through p27(Kip1), pRB relative p130, and altered E2F regulation. Mol Cell Biol 2000; 20:4745-53. PubMed
  • Baksh S, DeCaprio JA, Burakoff SJ. Calcineurin regulation of the mammalian G0/G1 checkpoint element, cyclin dependent kinase 4. Oncogene 2000; 19:2820-7. PubMed
  • Gjoerup O, Chao H, DeCaprio JA, Roberts TM. pRB-dependent, J domain-independent function of simian virus 40 large T antigen in override of p53 growth suppression. J Virol 2000; 74:864-74. PubMed
  • Dalal SN, Schweitzer CM, Gan J, DeCaprio JA. Cytoplasmic localization of human cdc25C during interphase requires an intact 14-3-3 binding site. Mol Cell Biol 1999; 19:4465-79. PubMed
  • Chauhan D, Hideshima T, Treon S, Teoh G, Raje N, Yoshihimito S, Tai YT, Li W, Fan J, DeCaprio J, Anderson KC. Functional interaction between retinoblastoma protein and stress-activated protein kinase in multiple myeloma cells. Cancer Res 1999; 59:1192-5. PubMed
  • Shim WS, DiRenzo J, DeCaprio JA, Santen RJ, Brown M, Jeng MH. Segregation of steroid receptor coactivator-1 from steroid receptors in mammary epithelium. Proc Natl Acad Sci U S A 1999; 96:208-13. PubMed
  • Marin MC, Jost CA, Irwin MS, DeCaprio JA, Caput D, Kaelin WG. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Mol Cell Biol 1998; 18:6316-24. PubMed
  • Maheswaran S, Englert C, Zheng G, Lee SB, Wong J, Harkin DP, Bean J, Ezzell R, Garvin AJ, McCluskey RT, DeCaprio JA, Haber DA. Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70. Genes Dev 1998; 12:1108-20. PubMed
  • Zalvide J, Stubdal H, DeCaprio JA. The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins. Mol Cell Biol 1998; 18:1408-15. PubMed
  • Sheng Q, Denis D, Ratnofsky M, Roberts TM, DeCaprio JA, Schaffhausen B. The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function. J Virol 1997; 71:9410-6. PubMed
  • Ayt. The fission yeast protein p73res2 is an essential component of the mitotic MBF complex and a master regulator of meiosis. Mol Cell Biol 1997; 17:6246-54. PubMed
  • Stubdal H, Zalvide J, Campbell KS, Schweitzer C, Roberts TM, DeCaprio JA. Inactivation of pRB-related proteins p130 and p107 mediated by the J domain of simian virus 40 large T antigen. Mol Cell Biol 1997; 17:4979-90. PubMed
  • Lill NL, Grossman SR, Ginsberg D, DeCaprio J, Livingston DM. Binding and modulation of p53 by p300/CBP coactivators. Nature 1997; 387:823-7. PubMed
  • Campbell KS, Mullane KP, Aksoy IA, Stubdal H, Zalvide J, Pipas JM, Silver PA, Roberts TM, Schaffhausen BS, DeCaprio JA. DnaJ/hsp40 chaperone domain of SV40 large T antigen promotes efficient viral DNA replication. Genes Dev 1997; 11:1098-110. PubMed
  • Peeper DS, Upton TM, Ladha MH, Neuman E, Zalvide J, Bernards R, DeCaprio JA, Ewen ME. Ras signalling linked to the cell-cycle machinery by the retinoblastoma protein. Nature 1997; 386:177-81. PubMed
  • Stubdal H, Zalvide J, DeCaprio JA. Simian virus 40 large T antigen alters the phosphorylation state of the RB-related proteins p130 and p107. J Virol 1996; 70:2781-8. PubMed
  • Zalvide J, DeCaprio JA. Role of pRb-related proteins in simian virus 40 large-T-antigen-mediated transformation. Mol Cell Biol 1995; 15:5800-10. PubMed
  • Kibel A, Iliopoulos O, DeCaprio JA, Kaelin WG. Binding of the von Hippel-Lindau tumor suppressor protein to Elongin B and C. Science 1995; 269:1444-6. PubMed
  • Ayt. The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Mol Cell Biol 1995; 15:2589-99. PubMed
  • Chittenden T, Livingston DM, DeCaprio JA. Cell cycle analysis of E2F in primary human T cells reveals novel E2F complexes and biochemically distinct forms of free E2F. Mol Cell Biol 1993; 13:3975-83. PubMed
  • Kaelin WG, Krek W, Sellers WR, DeCaprio JA, Ajchenbaum F, Fuchs CS, Chittenden T, Li Y, Farnham PJ, Blanar MA. Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. Cell 1992; 70:351-64. PubMed
  • DeCaprio JA, Furukawa Y, Ajchenbaum F, Griffin JD, Livingston DM. The retinoblastoma-susceptibility gene product becomes phosphorylated in multiple stages during cell cycle entry and progression. Proc Natl Acad Sci U S A 1992; 89:1795-8. PubMed
  • Ishida Y, Furukawa Y, Decaprio JA, Saito M, Griffin JD. Treatment of myeloid leukemic cells with the phosphatase inhibitor okadaic acid induces cell cycle arrest at either G1/S or G2/M depending on dose. J Cell Physiol 1992; 150:484-92. PubMed
  • Shirodkar S, Ewen M, DeCaprio JA, Morgan J, Livingston DM, Chittenden T. The transcription factor E2F interacts with the retinoblastoma product and a p107-cyclin A complex in a cell cycle-regulated manner. Cell 1992; 68:157-66. PubMed
  • DeCaprio JA, Mayer RJ, Gonin R, Arbuck SG. Fluorouracil and high-dose leucovorin in previously untreated patients with advanced adenocarcinoma of the pancreas: results of a phase II trial. J Clin Oncol 1991; 9:2128-33. PubMed
  • Furukawa Y, DeCaprio JA, Belvin M, Griffin JD. Heterogeneous expression of the product of the retinoblastoma susceptibility gene in primary human leukemia cells. Oncogene 1991; 6:1343-6. PubMed
  • Kaelin WG, Pallas DC, DeCaprio JA, Kaye FJ, Livingston DM. Identification of cellular proteins that can interact specifically with the T/E1A-binding region of the retinoblastoma gene product. Cell 1991; 64:521-32. PubMed
  • Laiho M, R. Control of junB and extracellular matrix protein expression by transforming growth factor-beta 1 is independent of simian virus 40 T antigen-sensitive growth-sensitive growth-inhibitory events. Mol Cell Biol 1991; 11:972-8. PubMed
  • Laiho M, DeCaprio JA, Ludlow JW, Livingston DM, Massagu. Growth inhibition by TGF-beta linked to suppression of retinoblastoma protein phosphorylation. Cell 1990; 62:175-85. PubMed
  • Furukawa Y, DeCaprio JA, Freedman A, Kanakura Y, Nakamura M, Ernst TJ, Livingston DM, Griffin JD. Expression and state of phosphorylation of the retinoblastoma susceptibility gene product in cycling and noncycling human hematopoietic cells. Proc Natl Acad Sci U S A 1990; 87:2770-4. PubMed
  • Ludlow JW, Shon J, Pipas JM, Livingston DM, DeCaprio JA. The retinoblastoma susceptibility gene product undergoes cell cycle-dependent dephosphorylation and binding to and release from SV40 large T. Cell 1990; 60:387-96. PubMed
  • DeCaprio JA, Ludlow JW, Lynch D, Furukawa Y, Griffin J, Piwnica-Worms H, Huang CM, Livingston DM. The product of the retinoblastoma susceptibility gene has properties of a cell cycle regulatory element. Cell 1989; 58:1085-95. PubMed
  • Ewen ME, Ludlow JW, Marsilio E, DeCaprio JA, Millikan RC, Cheng SH, Paucha E, Livingston DM. An N-terminal transformation-governing sequence of SV40 large T antigen contributes to the binding of both p110Rb and a second cellular protein, p120. Cell 1989; 58:257-67. PubMed
  • Ludlow JW, DeCaprio JA, Huang CM, Lee WH, Paucha E, Livingston DM. SV40 large T antigen binds preferentially to an underphosphorylated member of the retinoblastoma susceptibility gene product family. Cell 1989; 56:57-65. PubMed
Hide