Photo of Jeannie Lee,  MD, PhD

Jeannie Lee, MD, PhD

Massachusetts General Hospital

Massachusetts General Hospital

Jeannie Lee, MD, PhD

Massachusetts General Hospital

EDUCATIONAL TITLES

  • Professor, Genetics, Harvard Medical School
  • Professor of Genetics, Molecular Biology, Massachusetts General Hospital

DF/HCC PROGRAM AFFILIATION

Publications

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  • Minajigi A, Froberg JE, Wei C, Sunwoo H, Kesner B, Colognori D, Lessing D, Payer B, Boukhali M, Haas W, Lee JT. Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. Science 2015. PubMed
  • Pinter SF, Colognori D, Beliveau BJ, Sadreyev RI, Payer B, Yildirim E, Wu CT, Lee JT. Allelic Imbalance Is a Prevalent and Tissue-Specific Feature of the Mouse Transcriptome. Genetics 2015; 200:537-49. PubMed
  • Maier VK, Feeney CM, Taylor JE, Creech AL, Qiao JW, Szanto A, Das PP, Chevrier N, Cifuentes-Rojas C, Orkin SH, Carr SA, Jaffe JD, Mertins P, Lee JT. Functional proteomic analysis of repressive histone methyltransferase complexes PRC2 and G9A reveals ZNF518B as a G9A regulator. Mol Cell Proteomics 2015. PubMed
  • Davidovich C, Wang X, Cifuentes-Rojas C, Goodrich KJ, Gooding AR, Lee JT, Cech TR. Toward a consensus on the binding specificity and promiscuity of PRC2 for RNA. Mol Cell 2015; 57:552-8. PubMed
  • Kung JT, Kesner B, An JY, Ahn JY, Cifuentes-Rojas C, Colognori D, Jeon Y, Szanto A, del Rosario BC, Pinter SF, Erwin JA, Lee JT. Locus-specific targeting to the X chromosome revealed by the RNA interactome of CTCF. Mol Cell 2015; 57:361-75. PubMed
  • Creech AL, Taylor JE, Maier VK, Wu X, Feeney CM, Udeshi ND, Peach SE, Boehm JS, Lee JT, Carr SA, Jaffe JD. Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry. Methods 2015; 72:57-64. PubMed
  • Beliveau BJ, Boettiger AN, AvendaƱo MS, Jungmann R, McCole RB, Joyce EF, Kim-Kiselak C, Bantignies F, Fonseka CY, Erceg J, Hannan MA, Hoang HG, Colognori D, Lee JT, Shih WM, Yin P, Zhuang X, Wu CT. Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes. Nat Commun 2015; 6:7147. PubMed
  • Sarma K, Cifuentes-Rojas C, Ergun A, Del Rosario A, Jeon Y, White F, Sadreyev R, Lee JT. ATRX directs binding of PRC2 to Xist RNA and Polycomb targets. Cell 2014; 159:869-83. PubMed
  • Payer B, Lee JT. Coupling of X-Chromosome reactivation with the pluripotent stem cell state. RNA Biol 2014; 11:798-807. PubMed
  • Broering TJ, Alavattam KG, Sadreyev RI, Ichijima Y, Kato Y, Hasegawa K, Camerini-Otero RD, Lee JT, Andreassen PR, Namekawa SH. BRCA1 establishes DNA damage signaling and pericentric heterochromatin of the X chromosome in male meiosis. J Cell Biol 2014; 205:663-75. PubMed
  • Dimitrova N, Zamudio JR, Jong RM, Soukup D, Resnick R, Sarma K, Ward AJ, Raj A, Lee JT, Sharp PA, Jacks T. LincRNA-p21 Activates p21 In cis to Promote Polycomb Target Gene Expression and to Enforce the G1/S Checkpoint. Mol Cell 2014; 54:777-90. PubMed
  • Cifuentes-Rojas C, Hernandez AJ, Sarma K, Lee JT. Regulatory Interactions between RNA and Polycomb Repressive Complex 2. Mol Cell 2014. PubMed
  • Payer B, Rosenberg M, Yamaji M, Yabuta Y, Koyanagi-Aoi M, Hayashi K, Yamanaka S, Saitou M, Lee JT. Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming. Mol Cell 2013; 52:805-18. PubMed
  • Simon MD, Pinter SF, Fang R, Sarma K, Rutenberg-Schoenberg M, Bowman SK, Kesner BA, Maier VK, Kingston RE, Lee JT. High-resolution Xist binding maps reveal two-step spreading during X-chromosome inactivation. Nature 2013; 504:465-9. PubMed
  • Sun S, Del Rosario BC, Szanto A, Ogawa Y, Jeon Y, Lee JT. Jpx RNA activates Xist by evicting CTCF. Cell 2013; 153:1537-51. PubMed
  • Yildirim E, Kirby JE, Brown DE, Mercier FE, Sadreyev RI, Scadden DT, Lee JT. Xist RNA is a potent suppressor of hematologic cancer in mice. Cell 2013; 152:727-42. PubMed
  • Erwin JA, Del Rosario B, Payer B, Lee JT. An ex Vivo Model for Imprinting: Mutually Exclusive Binding of Cdx2 and Oct4 as a Switch for Imprinted and Random X-Inactivation. Genetics 2012; 192:857-68. PubMed
  • Pinter SF, Sadreyev RI, Yildirim E, Jeon Y, Ohsumi TK, Borowsky M, Lee JT. Spreading of X chromosome inactivation via a hierarchy of defined Polycomb stations. Genome Res 2012; 22:1864-76. PubMed
  • Anguera MC, Sadreyev R, Zhang Z, Szanto A, Payer B, Sheridan SD, Kwok S, Haggarty SJ, Sur M, Alvarez J, Gimelbrant A, Mitalipova M, Kirby JE, Lee JT. Molecular signatures of human induced pluripotent stem cells highlight sex differences and cancer genes. Cell Stem Cell 2012; 11:75-90. PubMed
  • Kim DH, Jeon Y, Anguera MC, Lee JT. X-chromosome epigenetic reprogramming in pluripotent stem cells via noncoding genes. Semin Cell Dev Biol 2011. PubMed
  • Namekawa SH, Lee JT. Detection of nascent RNA, single-copy DNA and protein localization by immunoFISH in mouse germ cells and preimplantation embryos. Nat Protoc 2011; 6:270-84. PubMed
  • Zhao J, Ohsumi TK, Kung JT, Ogawa Y, Grau DJ, Sarma K, Song JJ, Kingston RE, Borowsky M, Lee JT. Genome-wide identification of polycomb-associated RNAs by RIP-seq. Mol Cell 2010; 40:939-53. PubMed
  • Sarma K, Levasseur P, Aristarkhov A, Lee JT. Locked nucleic acids (LNAs) reveal sequence requirements and kinetics of Xist RNA localization to the X chromosome. Proc Natl Acad Sci U S A 2010; 107:22196-201. PubMed
  • Tian D, Sun S, Lee JT. The long noncoding RNA, Jpx, is a molecular switch for X chromosome inactivation. Cell 2010; 143:390-403. PubMed
  • Lee JT. The X as model for RNA's niche in epigenomic regulation. Cold Spring Harb Perspect Biol 2010; 2:a003749. PubMed
  • Namekawa SH, Payer B, Huynh KD, Jaenisch R, Lee JT. Two-step imprinted X inactivation: repeat versus genic silencing in the mouse. Mol Cell Biol 2010; 30:3187-205. PubMed
  • Lengner CJ, Gimelbrant AA, Erwin JA, Cheng AW, Guenther MG, Welstead GG, Alagappan R, Frampton GM, Xu P, Muffat J, Santagata S, Powers D, Barrett CB, Young RA, Lee JT, Jaenisch R, Mitalipova M. Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen concentrations. Cell 2010; 141:872-83. PubMed
  • Paro R, Lee JT. Extending the frontiers of epigenetic regulation. Curr Opin Genet Dev 2010; 20:107-9. PubMed
  • Erwin JA, Lee JT. Characterization of X-chromosome inactivation status in human pluripotent stem cells. Curr Protoc Stem Cell Biol 2010; Chapter 1:Unit 1B.6. PubMed
  • Sun S, Fukue Y, Nolen L, Sadreyev RI, Lee JT. Characterization of Xpr (Xpct) reveals instability but no effects on X-chromosome pairing or Xist expression. Transcr 2011; 1:46-56. PubMed
  • Ahn JY, Lee JT. Retinoic acid accelerates downregulation of the Xist repressor, Oct4, and increases the likelihood of Xist activation when Tsix is deficient. BMC Dev Biol 2010; 10:90. PubMed
  • Zhou D, Conrad C, Xia F, Park JS, Payer B, Yin Y, Lauwers GY, Thasler W, Lee JT, Avruch J, Bardeesy N. Mst1 and Mst2 maintain hepatocyte quiescence and suppress hepatocellular carcinoma development through inactivation of the Yap1 oncogene. Cancer Cell 2009; 16:425-38. PubMed
  • Lee JT. Lessons from X-chromosome inactivation: long ncRNA as guides and tethers to the epigenome. Genes Dev 2009; 23:1831-42. PubMed
  • Donohoe ME, Silva SS, Pinter SF, Xu N, Lee JT. The pluripotency factor Oct4 interacts with Ctcf and also controls X-chromosome pairing and counting. Nature 2009; 460:128-32. PubMed
  • Zhang L,Ogawa Y,Ahn J,Namekawa S,Silva S,Lee JT. Telomeric RNAs Mark Sex Chromosomes in Stem Cells. Genetics 2009; 182:685-98. PubMed
  • Namekawa SH,Lee JT. XY and ZW: is meiotic sex chromosome inactivation the rule in evolution? PLoS Genet 2009; 5:e1000493. PubMed
  • Anguera MC,Liu M,Avruch J,Lee JT. Characterization of two Mst1-deficient mouse models. Dev Dyn 2008; 237:3424-34. PubMed
  • Zhao J,Sun BK,Erwin JA,Song JJ,Lee JT. Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome. Science 2008; 322:750-6. PubMed
  • Lee JT. Jeannie T. Lee. Curr Biol 2008; 18:R940-1. PubMed
  • Tsai CL,Rowntree RK,Cohen DE,Lee JT. Higher order chromatin structure at the X-inactivation center via looping DNA. Dev Biol 2008; 319:416-25. PubMed
  • Ogawa Y,Sun BK,Lee JT. Intersection of the RNA interference and X-inactivation pathways. Science 2008; 320:1336-41. PubMed
  • Erwin JA,Lee JT. New twists in X-chromosome inactivation. Curr Opin Cell Biol 2008; 20:349-55. PubMed
  • Silva SS, Rowntree RK, Mekhoubad S, Lee JT. X-chromosome inactivation and epigenetic fluidity in human embryonic stem cells. Proc Natl Acad Sci U S A 2008; 105:4820-5. PubMed
  • Lindroth AM,Park YJ,McLean CM,Dokshin GA,Persson JM,Herman H,Pasini D,Miro X,Donohoe ME,Lee JT,Helin K,Soloway PD. Antagonism between DNA and H3K27 methylation at the imprinted Rasgrf1 locus. PLoS Genet 2008; 4:e1000145. PubMed
  • Lin H, Gupta V, Vermilyea MD, Falciani F, Lee JT, O'Neill LP, Turner BM. Dosage compensation in the mouse balances up-regulation and silencing of X-linked genes. PLoS Biol 2007; 5:e326. PubMed
  • Xu N, Donohoe ME, Silva SS, Lee JT. Evidence that homologous X-chromosome pairing requires transcription and Ctcf protein. Nat Genet 2007; 39:1390-6. PubMed
  • Namekawa SH, Vandeberg JL, McCarrey JR, Lee JT. Sex chromosome silencing in the marsupial male germ line. Proc Natl Acad Sci U S A 2007; 104:9730-5. PubMed
  • Zhang LF, Huynh KD, Lee JT. Perinucleolar targeting of the inactive X during S phase: evidence for a role in the maintenance of silencing. Cell 2007; 129:693-706. PubMed
  • Mikkelsen TS, Wakefield MJ, Aken B, Amemiya CT, Chang JL, Duke S, Garber M, Gentles AJ, Goodstadt L, Heger A, Jurka J, Kamal M, Mauceli E, Searle SM, Sharpe T, Baker ML, Batzer MA, Benos PV, Belov K, Clamp M, Cook A, Cuff J, Das R, Davidow L, Deakin JE, Fazzari MJ, Glass JL, Grabherr M, Greally JM, Gu W, Hore TA, Huttley GA, Kleber M, Jirtle RL, Koina E, Lee JT, Mahony S, Marra MA, Miller RD, Nicholls RD, Oda M, Papenfuss AT, Parra ZE, Pollock DD, Ray DA, Schein JE, Speed TP, Thompson K, VandeBerg JL, Wade CM, Walker JA, Waters PD, Webber C, Weidman JR, Xie X, Zody MC, Graves JA, Ponting CP, Breen M, Samollow PB, Lander ES, Lindblad-Toh K. Genome of the marsupial Monodelphis domestica reveals innovation in non-coding sequences. Nature 2007; 447:167-77. PubMed
  • Kim S, Namekawa SH, Niswander LM, Ward JO, Lee JT, Bardwell VJ, Zarkower D. A mammal-specific Doublesex homolog associates with male sex chromatin and is required for male meiosis. PLoS Genet 2007; 3:e62. PubMed
  • Donohoe ME, Zhang LF, Xu N, Shi Y, Lee JT. Identification of a Ctcf cofactor, Yy1, for the X chromosome binary switch. Mol Cell 2007; 25:43-56. PubMed
  • Davidow LS, Breen M, Duke SE, Samollow PB, McCarrey JR, Lee JT. The search for a marsupial XIC reveals a break with vertebrate synteny. Chromosome Res 2007; 15:137-46. PubMed
  • Cohen DE, Davidow LS, Erwin JA, Xu N, Warshawsky D, Lee JT. The DXPas34 repeat regulates random and imprinted X inactivation. Dev Cell 2007; 12:57-71. PubMed
  • Lewis A, Green K, Dawson C, Redrup L, Huynh KD, Lee JT, Hemberger M, Reik W. Epigenetic dynamics of the Kcnq1 imprinted domain in the early embryo. Development 2006; 133:4203-10. PubMed
  • Rowntree RK, Lee JT. Mapping of DNA replication origins to noncoding genes of the X-inactivation center. Mol Cell Biol 2006; 26:3707-17. PubMed
  • Sun BK, Deaton AM, Lee JT. A Transient Heterochromatic State in Xist Preempts X Inactivation Choice without RNA Stabilization. Mol Cell 2006; 21:617-628. PubMed
  • Boumil RM, Ogawa Y, Sun BK, Huynh KD, Lee JT. Differential methylation of Xite and CTCF sites in Tsix mirrors the pattern of X-inactivation choice in mice. Mol Cell Biol 2006; 26:2109-17. PubMed
  • Xu N, Tsai CL, Lee JT. Transient homologous chromosome pairing marks the onset of X inactivation. Science 2006; 311:1149-52. PubMed
  • Lee JT. Regulation of X-chromosome counting by Tsix and Xite sequences. Science 2005; 309:768-71. PubMed
  • Shibata S, Lee JT. Tsix transcription- versus RNA-based mechanisms in Xist repression and epigenetic choice. Curr Biol 2004; 14:1747-54. PubMed
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