Photo of Juan Melero-Martin,

Juan Melero-Martin

Boston Children's Hospital

Boston Children's Hospital
Phone: (617) 919-3072


juan.meleromartin@childrens.harvard.edu

Juan Melero-Martin

Boston Children's Hospital

EDUCATIONAL TITLES

  • Assistant Professor, Surgery, Harvard Medical School
  • Associate Scientific Research, Cardiac Surgery, Boston Children's Hospital

Research Abstract

The goal of my laboratory is to develop cell-based technologies that will allow engineering vascularized tissues in vivo using postnatal progenitor cells obtained from patients by non-invasive means. In particular, our focus is on the combined use of bone marrow-derived mesenchymal progenitor cells (MPCs) and human blood-derived endothelial progenitor cells (EPCs) to regenerate adipose and skeletal muscle tissues in vivo lost after tumor resections (e.g., mastectomy and carcinoma removal). The research conducted in my laboratory is trying to address whether generating a vascular implants with highly purified and defined MPCs and EPCs will drive tissue development in vivo. We give priority to the initial establishment of a functional vascular network within the implant, and propose that as a result of this neo-vascularization appropriate tissue formation and remodeling will be achieved.

Publications

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  • Chuang CH, Lin RZ, Tien HW, Chu YC, Li YC, Melero-Martin JM, Chen YC. Enzymatic regulation of functional vascular networks using gelatin hydrogels. Acta Biomater 2015. PubMed
  • Lin RZ, Moreno-Luna R, Li D, Jaminet SC, Greene AK, Melero-Martin JM. Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling. Proc Natl Acad Sci U S A 2014. PubMed
  • Muñoz-Hernandez R, Miranda ML, Stiefel P, Lin RZ, Praena-Fernández JM, Dominguez-Simeon MJ, Villar J, Moreno-Luna R, Melero-Martin JM. Decreased Level of Cord Blood Circulating Endothelial Colony-Forming Cells in Preeclampsia. Hypertension 2014. PubMed
  • Moreno-Luna R, Muñoz-Hernandez R, Lin RZ, Miranda ML, Vallejo-Vaz AJ, Stiefel P, Praena-Fernández JM, Bernal-Bermejo J, Jimenez-Jimenez LM, Villar J, Melero-Martin JM. Maternal Body-Mass Index and Cord Blood Circulating Endothelial Colony-Forming Cells. J Pediatr 2013. PubMed
  • Lin RZ, Moreno-Luna R, Muñoz-Hernandez R, Li D, Jaminet SC, Greene AK, Melero-Martin JM. Human white adipose tissue vasculature contains endothelial colony-forming cells with robust in vivo vasculogenic potential. Angiogenesis 2013. PubMed
  • Lin RZ, Moreno-Luna R, Zhou B, Pu WT, Melero-Martin JM. Equal modulation of endothelial cell function by four distinct tissue-specific mesenchymal stem cells. Angiogenesis 2012; 15:443-55. PubMed
  • Wagner M, Bjerkvig R, Wiig H, Melero-Martin JM, Lin RZ, Klagsbrun M, Dudley AC. Inflamed tumor-associated adipose tissue is a depot for macrophages that stimulate tumor growth and angiogenesis. Angiogenesis 2012; 15:481-95. PubMed
  • Chen YC, Lin RZ, Qi H, Yang Y, Bae H, Melero-Martin JM, Khademhosseini A. Functional Human Vascular Network Generated in Photocrosslinkable Gelatin Methacrylate Hydrogels. Adv Funct Mater 2012; 22:2027-2039. PubMed
  • Lin RZ, Melero-Martin JM. Fibroblast growth factor-2 facilitates rapid anastomosis formation between bioengineered human vascular networks and living vasculature. Methods 2012. PubMed
  • Lin RZ, Dreyzin A, Aamodt K, Li D, Jaminet SC, Dudley AC, Melero-Martin JM. Induction of erythropoiesis using human vascular networks genetically-engineered for controlled erythropoietin release. Blood 2011. PubMed
  • Zhou B, Honor LB, Ma Q, Oh JH, Lin RZ, Melero-Martin JM, von Gise A, Zhou P, Hu T, He L, Wu KH, Zhang H, Zhang Y, Pu WT. Thymosin beta 4 treatment after myocardial infarction does not reprogram epicardial cells into cardiomyocytes. J Mol Cell Cardiol 2011. PubMed
  • Zhou B, Honor LB, He H, Ma Q, Oh JH, Butterfield C, Lin RZ, Melero-Martin JM, Dolmatova E, Duffy HS, Gise A, Zhou P, Hu YW, Wang G, Zhang B, Wang L, Hall JL, Moses MA, McGowan FX, Pu WT. Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest 2011; 121:1894-904. PubMed
  • Allen P, Melero-Martin J, Bischoff J. Type I collagen, fibrin and PuraMatrix matrices provide permissive environments for human endothelial and mesenchymal progenitor cells to form neovascular networks. 2011; 5:e74-86. PubMed
  • Melero-Martin JM, Dudley AC. Concise review: Vascular stem cells and tumor angiogenesis. Stem Cells 2011; 29:163-8. PubMed
  • Lin RZ, Melero-Martin JM. Bioengineering human microvascular networks in immunodeficient mice. 2011. PubMed
  • Dudley AC, Udagawa T, Melero-Martin JM, Shih SC, Curatolo A, Moses MA, Klagsbrun M. Bone marrow is a reservoir for pro-angiogenic myelomonocytic cells but not endothelial cells in spontaneous tumors. Blood 2010; 116:3367-71. PubMed
  • Lin RZ, Dreyzin A, Aamodt K, Dudley AC, Melero-Martin JM. Functional endothelial progenitor cells from cryopreserved umbilical cord blood. Cell Transplant 2010. PubMed
  • Hjortnaes J, Gottlieb D, Figueiredo JL, Melero-Martin J, Kohler RH, Bischoff J, Weissleder R, Mayer J, Aikawa E. Intravital Molecular Imaging of Small-Diameter Tissue-Engineered Vascular Grafts: A Feasibility Study. 2010; 16:597-607. PubMed
  • Melero-Martin J, De Obaldia ME, Allen P, Dudley AC, Klagsbrun M, Bischoff J. Host myeloid cells are necessary for creating bio-engineered human vascular networks in vivo. Tissue Eng Part A 2010; 16:2457-66. PubMed
  • Gottlieb D, Kunal T, Emani S, Aikawa E, Brown DW, Powell AJ, Nedder A, Engelmayr GC, Melero-Martin JM, Sacks MS, Mayer JE. In vivo monitoring of function of autologous engineered pulmonary valve. J Thorac Cardiovasc Surg 2010; 139:723-31. PubMed
  • Melero-Martin JM,Santhalingam S,Al-Rubeai M. Methodology for optimal in vitro cell expansion in tissue engineering. Adv Biochem Eng Biotechnol 2009; 112:209-29. PubMed
  • Melero-Martin JM,De Obaldia ME,Kang SY,Khan ZA,Yuan L,Oettgen P,Bischoff J. Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells. Circ Res 2008; 103:194-202. PubMed
  • Khan ZA,Boscolo E,Picard A,Psutka S,Melero-Martin JM,Bartch TC,Mulliken JB,Bischoff J. Multipotential stem cells recapitulate human infantile hemangioma in immunodeficient mice. J Clin Invest 2008; 118:2592-9. PubMed
  • Melero-Martin JM,Bischoff J. Chapter 13. An in vivo experimental model for postnatal vasculogenesis. Methods Enzymol 2008; 445:303-29. PubMed
  • Melero-Martin JM, Khan ZA, Picard A, Wu X, Paruchuri S, Bischoff J. In vivo vasculogenic potential of human blood-derived endothelial progenitor cells. Blood 2007; 109:4761-8. PubMed
  • Paruchuri S, Yang JH, Aikawa E, Melero-Martin JM, Khan ZA, Loukogeorgakis S, Schoen FJ, Bischoff J. Human pulmonary valve progenitor cells exhibit endothelial/mesenchymal plasticity in response to vascular endothelial growth factor-A and transforming growth factor-beta2. Circ Res 2006; 99:861-9. PubMed
  • Khan ZA, Melero-Martin JM, Wu X, Paruchuri S, Boscolo E, Mulliken JB, Bischoff J. Endothelial progenitor cells from infantile hemangioma and umbilical cord blood display unique cellular responses to endostatin. Blood 2006; 108:915-21. PubMed
  • Melero-Martin JM,Dowling MA,Smith M,Al-Rubeai M. Expansion of chondroprogenitor cells on macroporous microcarriers as an alternative to conventional monolayer systems. Biomaterials 2006; 27:2970-9. PubMed
  • Melero-Martin JM,Dowling MA,Smith M,Al-Rubeai M. Optimal in-vitro expansion of chondroprogenitor cells in monolayer culture. Biotechnol Bioeng 2005; 93:519-33. PubMed
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