Kathryn D. Held, PhD

Massachusetts General Hospital

Massachusetts General Hospital
Phone: (617) 726-8161
Fax: (617) 724-8320


kheld@partners.org

Kathryn D. Held, PhD

Massachusetts General Hospital

EDUCATIONAL TITLES

  • Associate Professor, Radiation Oncology, Harvard Medical School
  • Associate Radiation Biologist, Radiation Oncology, Massachusetts General Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

Our research covers two related areas: cellular responses to localized oxidative stress and mechanisms for stress-induced bystander effects. The studies on localized oxidative stress involve a team approach for use of state-of-the-art methods such as ionizing radiation microbeams, in collaboration with a group in the UK, and subcellularly localized photosensitizers to study biochemical pathways involved in triggering of apoptosis, mutagenesis, and DNA damage and repair after subcellularly localized stress induction, e.g., in nuclear versus non-nuclear regions including plasma membranes and mitochondria. Cells containing genetic mutations or knock-downs in apoptosis and DNA repair pathways are used, and cellular responses are followed, among other approaches, by use of sophisticated time-lapse microscopy. The studies on stress-induced bystander effects involve investigation of the signaling pathways whereby a cell that is damaged by ionizing radiation, photosensitizers or other oxidative stresses sends a signal to neighboring undamaged cells which then show a biological response. These studies include use of a unique heavy ion irradiation facility at Brookhaven National Laboratory.

Publications

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  • Liu Q, Wang M, Kern AM, Khaled S, Han J, Yeap BY, Hong TS, Settleman J, Benes CH, Held KD, Efstathiou JA, Willers H. Adapting a drug screening platform to discover associations of molecular targeted radiosensitizers with genomic biomarkers. Mol Cancer Res 2015. PubMed
  • Liu Q, Ghosh P, Magpayo N, Testa M, Tang S, Gheorghiu L, Biggs P, Paganetti H, Efstathiou JA, Lu HM, Held KD, Willers H. Lung cancer cell line screen links fanconi anemia/BRCA pathway defects to increased relative biological effectiveness of proton radiation. Int J Radiat Oncol Biol Phys 2015; 91:1081-9. PubMed
  • Kesler CT, Kuo AH, Wong HK, Masuck DJ, Shah JL, Kozak KR, Held KD, Padera TP. Vascular endothelial growth factor-C enhances radiosensitivity of lymphatic endothelial cells. Angiogenesis 2014; 17:419-27. PubMed
  • Liu Q, Xu C, Kirubakaran S, Zhang X, Hur W, Liu Y, Kwiatkowski NP, Wang J, Westover KD, Gao P, Ercan D, Niepel M, Thoreen CC, Kang SA, Patricelli MP, Wang Y, Tupper T, Altabef A, Kawamura H, Held KD, Chou DM, Elledge SJ, Janne PA, Wong KK, Sabatini DM, Gray NS. Characterization of Torin2, an ATP-competitive inhibitor of mTOR, ATM, and ATR. Cancer Res 2013; 73:2574-86. PubMed
  • Held KD. Summary: achievements, critical issues, and thoughts on the future. Health Phys 2012; 103:681-4. PubMed
  • Butterworth KT, McGarry CK, Clasie B, Carabe-Fernandez A, Schuemann J, Depauw N, Tang S, McMahon SJ, Schettino G, O'Sullivan JM, Lu HM, Kooy H, Paganetti H, Hounsell AR, Held KD, Prise KM. Relative biological effectiveness (RBE) and out-of-field cell survival responses to passive scattering and pencil beam scanning proton beam deliveries. Phys Med Biol 2012; 57:6671-80. PubMed
  • Wakatsuki M, Magpayo N, Kawamura H, Held KD. Differential bystander signaling between radioresistant chondrosarcoma cells and fibroblasts after x-ray, proton, iron ion and carbon ion exposures. Int J Radiat Oncol Biol Phys 2012; 84:e103-8. PubMed
  • Yang H, Magpayo N, Held KD. Targeted and non-targeted effects from combinations of low doses of energetic protons and iron ions in human fibroblasts. Int J Radiat Biol 2011; 87:311-9. PubMed
  • Purschke M, Rubio N, Held KD, Redmond RW. Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy. Photochem Photobiol Sci 2010; 9:1634-9. PubMed
  • Held KD. Basic Clinical Radiobiology. Int J Radiat Biol 2010. PubMed
  • Zhang Y, Zhou J, Baldwin J, Held KD, Prise KM, Redmond RW, Liber HL. Ionizing radiation-induced bystander mutagenesis and adaptation: quantitative and temporal aspects. Mutat Res 2009; 671:20-5. PubMed
  • Rosenstein BS, Held KD, Rockwell S, Williams JP, Zeman EM. American Society for Radiation Oncology (ASTRO) survey of radiation biology educators in U.S. and Canadian radiation oncology residency programs. Int J Radiat Oncol Biol Phys 2009; 75:896-905. PubMed
  • Rubio N,Rajadurai A,Held KD,Prise KM,Liber HL,Redmond RW. Real-time imaging of novel spatial and temporal responses to photodynamic stress. Free Radic Biol Med 2009; 47:283-90. PubMed
  • Chakraborty A, Held KD, Prise KM, Liber HL, Redmond RW. Bystander effects induced by diffusing mediators after photodynamic stress. Radiat Res 2009; 172:74-81. PubMed
  • Kuhnert VM,Kachnic LA,Li L,Purschke M,Gheorghiu L,Lee R,Held KD,Willers H. FANCD2-deficient human fibroblasts are hypersensitive to ionising radiation at oxygen concentrations of 0% and 3% but not under normoxic conditions. Int J Radiat Biol 2009; 85:523-31. PubMed
  • Held KD. Effects of low fluences of radiations found in space on cellular systems. Int J Radiat Biol 2009; 85:379-90. PubMed
  • Zhang Y, Zhou J, Held KD, Redmond RW, Prise KM, Liber HL. Deficiencies of double-strand break repair factors and effects on mutagenesis in directly gamma-irradiated and medium-mediated bystander human lymphoblastoid cells. Radiat Res 2008; 169:197-206. PubMed
  • Shao C,Folkard M,Held KD,Prise KM. Estrogen enhanced cell-cell signalling in breast cancer cells exposed to targeted irradiation. BMC Cancer 2008; 8:184. PubMed
  • Lawenda BD, Smith DE, Xu L, Niemierko A, Silverstein JR, Boucher Y, Kashiwagi S, Held KD, Jain RK, Loeffler JS, Eisenberg DM, Blumberg JB. Do the dietary supplements epigallocatechin gallate or vitamin e cause a radiomodifying response on tumors in vivo? A pilot study with murine breast carcinoma. J Soc Integr Oncol 2007; 5:11-7. PubMed
  • Yang H, Anzenberg V, Held KD. The time dependence of bystander responses induced by iron-ion radiation in normal human skin fibroblasts. Radiat Res 2007; 168:292-8. PubMed
  • Willers H, Held KD. Introduction to clinical radiation biology. Hematol Oncol Clin North Am 2006; 20:1-24. PubMed
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