Photo of Leonard I. Zon,  MD

Leonard I. Zon, MD

Boston Children's Hospital

Boston Children's Hospital
Phone: (617) 919-2069
Fax: (617) 730-0222


zon@enders.tch.harvard.edu

Leonard I. Zon, MD

Boston Children's Hospital

EDUCATIONAL TITLES

  • Grousbeck Professor of Pediatrics, Pediatrics, Harvard Medical School
  • Professor, Stem Cell and Regenerative Biology, Harvard Medical School
  • Investigator, Howard Hughes Medical Institute, Boston Children's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

Research Contributions:

The laboratory focuses on the developmental biology of hematopoiesis and cancer. We have collected over 30 mutants affecting the hematopoietic system. Some of the mutants represent excellent animal models of human disease. We also have undertaken chemical genetic approach to blood development and have found that prostaglandins upregulates blood stem cells. This has led to a clinical trial to improve engraftment for patients receiving cord blood transplants. We recently developed suppressor screening genetics and found that transcriptional elongation regulates blood cell fate.

The laboratory has also developed zebrafish models of cancer. We have generated a melanoma model in the zebrafish system using transgenics. Transgenic fish get nevi, and in a combination with a p53 mutant fish develop melanomas. We recently found a histone methyltransferase that can accelerate melanoma, and discovered a small molecule that blocks transcription elongation and suppresses melanoma growth.

Publications

Powered by Harvard Catalyst
  • Svoboda O, Stachura DL, Machonova O, Zon LI, Traver D, Bartunek P. Ex vivo tools for the clonal analysis of zebrafish hematopoiesis. Nat Protoc 2016; 11:1007-1020. PubMed
  • Wakabayashi A, Ulirsch JC, Ludwig LS, Fiorini C, Yasuda M, Choudhuri A, McDonel P, Zon LI, Sankaran VG. Insight into GATA1 transcriptional activity through interrogation of cis elements disrupted in human erythroid disorders. Proc Natl Acad Sci U S A 2016; 113:4434-9. PubMed
  • Tan JL, Fogley RD, Flynn RA, Ablain J, Yang S, Saint-André V, Fan ZP, Do BT, Laga AC, Fujinaga K, Santoriello C, Greer CB, Kim YJ, Clohessy JG, Bothmer A, Pandell N, Avagyan S, Brogie JE, van Rooijen E, Hagedorn EJ, Shyh-Chang N, White RM, Price DH, Pandolfi PP, Peterlin BM, Zhou Y, Kim TH, Asara JM, Chang HY, Young RA, Zon LI. Stress from Nucleotide Depletion Activates the Transcriptional Regulator HEXIM1 to Suppress Melanoma. Mol Cell 2016; 62:34-46. PubMed
  • Avagyan S, Zon LI. Fish to Learn: Insights into Blood Development and Blood Disorders from Zebrafish Hematopoiesis. Hum Gene Ther 2016; 27:287-94. PubMed
  • Gao X, Wu T, Johnson KD, Lahvic JL, Ranheim EA, Zon LI, Bresnick EH. GATA Factor-G-Protein-Coupled Receptor Circuit Suppresses Hematopoiesis. Stem Cell Reports 2016; 6:368-82. PubMed
  • Kaufman CK, Mosimann C, Fan ZP, Yang S, Thomas AJ, Ablain J, Tan JL, Fogley RD, van Rooijen E, Hagedorn EJ, Ciarlo C, White RM, Matos DA, Puller AC, Santoriello C, Liao EC, Young RA, Zon LI. A zebrafish melanoma model reveals emergence of neural crest identity during melanoma initiation. Science 2016; 351:aad2197. PubMed
  • Huang J, Liu X, Li D, Shao Z, Cao H, Zhang Y, Trompouki E, Bowman TV, Zon LI, Yuan GC, Orkin SH, Xu J. Dynamic Control of Enhancer Repertoires Drives Lineage and Stage-Specific Transcription during Hematopoiesis. Dev Cell 2016; 36:9-23. PubMed
  • Heilmann S, Ratnakumar K, Langdon EM, Kansler ER, Kim IS, Campbell NR, Perry EB, McMahon AJ, Kaufman CK, van Rooijen E, Lee W, Iacobuzio-Donahue CA, Hynes RO, Zon LI, Xavier JB, White RM. A Quantitative System for Studying Metastasis Using Transparent Zebrafish. Cancer Res 2015. PubMed
  • Anderson H, Patch TC, Reddy PN, Hagedorn EJ, Kim PG, Soltis KA, Chen MJ, Tamplin OJ, Frye M, MacLean GA, Hübner K, Bauer DE, Kanki JP, Vogin G, Huston NC, Nguyen M, Fujiwara Y, Paw BH, Vestweber D, Zon LI, Orkin SH, Daley GQ, Shah DI. Hematopoietic stem cells develop in the absence of endothelial cadherin 5 expression. Blood 2015. PubMed
  • Li P, Lahvic JL, Binder V, Pugach EK, Riley EB, Tamplin OJ, Panigrahy D, Bowman TV, Barrett FG, Heffner GC, McKinney-Freeman S, Schlaeger TM, Daley GQ, Zeldin DC, Zon LI. Epoxyeicosatrienoic acids enhance embryonic haematopoiesis and adult marrow engraftment. Nature 2015; 523:468-71. PubMed
  • Dang M, Zon LI. Screening for melanoma resistance genes in vivo. Pigment Cell Melanoma Res 2015; 28:375-6. PubMed
  • Gao L, Li D, Ma K, Zhang W, Xu T, Fu C, Jing C, Jia X, Wu S, Sun X, Dong M, Deng M, Chen Y, Zhu W, Peng J, Wan F, Zhou Y, Zon LI, Pan W. TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. PLoS Genet. 2015; 11:e1005346. PubMed
  • Kim PG, Nakano H, Das PP, Chen MJ, Rowe RG, Chou SS, Ross SJ, Sakamoto KM, Zon LI, Schlaeger TM, Orkin SH, Nakano A, Daley GQ. Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence. J Exp Med 2015; 212:633-48. PubMed
  • Jing L, Tamplin OJ, Chen MJ, Deng Q, Patterson S, Kim PG, Durand EM, McNeil A, Green JM, Matsuura S, Ablain J, Brandt MK, Schlaeger TM, Huttenlocher A, Daley GQ, Ravid K, Zon LI. Adenosine signaling promotes hematopoietic stem and progenitor cell emergence. J Exp Med 2015; 212:649-63. PubMed
  • Ablain J, Durand EM, Yang S, Zhou Y, Zon LI. A CRISPR/Cas9 vector system for tissue-specific gene disruption in zebrafish. Dev Cell 2015; 32:756-64. PubMed
  • Gjini E, Mansour MR, Sander JD, Moritz N, Nguyen AT, Kesarsing M, Gans E, He S, Chen S, Ko M, Kuang YY, Yang S, Zhou Y, Rodig S, Zon LI, Joung JK, Rao A, Look AT. A zebrafish model of myelodysplastic syndrome produced through tet2 genomic editing. Mol Cell Biol 2015; 35:789-804. PubMed
  • Jang IH, Lu YF, Zhao L, Wenzel PL, Kume T, Datta SM, Arora N, Guiu J, Lagha M, Kim PG, Do EK, Kim JH, Schlaeger TM, Zon LI, Bigas A, Burns CE, Daley GQ. Notch1 acts via Foxc2 to promote definitive hematopoiesis via effects on hemogenic endothelium. Blood 2015; 125:1418-26. PubMed
  • Tamplin OJ, Durand EM, Carr LA, Childs SJ, Hagedorn EJ, Li P, Yzaguirre AD, Speck NA, Zon LI. Hematopoietic stem cell arrival triggers dynamic remodeling of the perivascular niche. Cell 2015; 160:241-52. PubMed
  • Schlaeger TM, Daheron L, Brickler TR, Entwisle S, Chan K, Cianci A, DeVine A, Ettenger A, Fitzgerald K, Godfrey M, Gupta D, McPherson J, Malwadkar P, Gupta M, Bell B, Doi A, Jung N, Li X, Lynes MS, Brookes E, Cherry AB, Demirbas D, Tsankov AM, Zon LI, Rubin LL, Feinberg AP, Meissner A, Cowan CA, Daley GQ. A comparison of non-integrating reprogramming methods. Nat Biotechnol 2015; 33:58-63. PubMed
  • Liu X, Jia X, Yuan H, Ma K, Chen Y, Jin Y, Deng M, Pan W, Chen S, Chen Z, de The H, Zon LI, Zhou Y, Zhou J, Zhu J. DNA methyltransferase 1 functions through C/ebpa to maintain hematopoietic stem and progenitor cells in zebrafish. J Hematol Oncol 2015; 8:15. PubMed
  • Lin MI, Price EN, Boatman S, Hagedorn EJ, Trompouki E, Satishchandran S, Carspecken CW, Uong A, DiBiase A, Yang S, Canver MC, Dahlberg A, Lu Z, Zhang CC, Orkin SH, Bernstein ID, Aster JC, White RM, Zon LI. Angiopoietin-like proteins stimulate HSPC development through interaction with notch receptor signaling. Elife 2015. PubMed
  • Hagedorn EJ, Durand EM, Fast EM, Zon LI. Getting more for your marrow: boosting hematopoietic stem cell numbers with PGE2. Exp Cell Res 2014. PubMed
  • Thornton JE, Du P, Jing L, Sjekloca L, Lin S, Grossi E, Sliz P, Zon LI, Gregory RI. Selective microRNA uridylation by Zcchc6 (TUT7) and Zcchc11 (TUT4). Nucleic Acids Res 2014; 42:11777-91. PubMed
  • Guo C, Chen X, Song H, Maynard MA, Zhou Y, Lobanov AV, Gladyshev VN, Ganis JJ, Wiley D, Jugo RH, Lee NY, Castroneves LA, Zon LI, Scanlan TS, Feldman HA, Huang SA. Intrinsic expression of a multiexon type 3 deiodinase gene controls zebrafish embryo size. Endocrinology 2014; 155:4069-80. PubMed
  • Svoboda O, Stachura DL, Machoňová O, Pajer P, Brynda J, Zon LI, Traver D, Bartůněk P. Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Blood 2014. PubMed
  • Kong Y, Grimaldi M, Curtin E, Dougherty M, Kaufman C, White RM, Zon LI, Liao EC. Neural crest development and craniofacial morphogenesis is coordinated by nitric oxide and histone acetylation. Chem Biol 2014; 21:488-501. PubMed
  • Zhang X, Ling J, Barcia G, Jing L, Wu J, Barry BJ, Mochida GH, Hill RS, Weimer JM, Stein Q, Poduri A, Partlow JN, Ville D, Dulac O, Yu TW, Lam AT, Servattalab S, Rodriguez J, Boddaert N, Munnich A, Colleaux L, Zon LI, Söll D, Walsh CA, Nabbout R. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet 2014; 94:547-58. PubMed
  • Alcaraz-Pérez F, García-Castillo J, García-Moreno D, López-Muñoz A, Anchelin M, Angosto D, Zon LI, Mulero V, Cayuela ML. A non-canonical function of telomerase RNA in the regulation of developmental myelopoiesis in zebrafish. Nat Commun 2014; 5:3228. PubMed
  • Stachura DL, Svoboda O, Campbell CA, Espín-Palazón R, Lau RP, Zon LI, Bartunek P, Traver D. The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 paralogous cytokines and their roles in hematopoietic development and maintenance. Blood 2013; 122:3918-28. PubMed
  • Ablain J, Zon LI. Of fish and men: using zebrafish to fight human diseases. Trends Cell Biol 2013; 23:584-6. PubMed
  • Boatman S, Barrett F, Satishchandran S, Jing L, Shestopalov I, Zon LI. Assaying hematopoiesis using zebrafish. Blood Cells Mol Dis 2013; 51:271-6. PubMed
  • Chen CH, Durand E, Wang J, Zon LI, Poss KD. zebraflash transgenic lines for in vivo bioluminescence imaging of stem cells and regeneration in adult zebrafish. Development 2013; 140:4988-97. PubMed
  • Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI. A network of epigenetic regulators guides developmental haematopoiesis in vivo. Nat Cell Biol 2013; 15:1516-25. PubMed
  • Xu C, Tabebordbar M, Iovino S, Ciarlo C, Liu J, Castiglioni A, Price E, Liu M, Barton ER, Kahn CR, Wagers AJ, Zon LI. A zebrafish embryo culture system defines factors that promote vertebrate myogenesis across species. Cell 2013; 155:909-21. PubMed
  • Cutler C, Multani P, Robbins D, Kim HT, Le T, Hoggatt J, Pelus LM, Desponts C, Chen YB, Rezner B, Armand P, Koreth J, Glotzbecker B, Ho VT, Alyea E, Isom M, Kao G, Armant M, Silberstein L, Hu P, Soiffer RJ, Scadden DT, Ritz J, Goessling W, North TE, Mendlein J, Ballen K, Zon LI, Antin JH, Shoemaker DD. Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation. Blood 2013; 122:3074-81. PubMed
  • Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, Bernstein ID, Collins JJ, Zon LI, Daley GQ. Induction of multipotential hematopoietic progenitors from human pluripotent stem cells via respecification of lineage-restricted precursors. Cell Stem Cell 2013; 13:459-70. PubMed
  • Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI. Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system. Dev Dyn 2013; 242:949-63. PubMed
  • Storer NY, White RM, Uong A, Price E, Nielsen GP, Langenau DM, Zon LI. Zebrafish rhabdomyosarcoma reflects the developmental stage of oncogene expression during myogenesis. Development 2013; 140:3040-50. PubMed
  • Le X, Pugach EK, Hettmer S, Storer NY, Liu J, Wills AA, DiBiase A, Chen EY, Ignatius MS, Poss KD, Wagers AJ, Langenau DM, Zon LI. A novel chemical screening strategy in zebrafish identifies common pathways in embryogenesis and rhabdomyosarcoma development. Development 2013. PubMed
  • Howe K, Clark MD, Torroja CF, Torrance J, Berthelot C, Muffato M, Collins JE, Humphray S, McLaren K, Matthews L, McLaren S, Sealy I, Caccamo M, Churcher C, Scott C, Barrett JC, Koch R, Rauch GJ, White S, Chow W, Kilian B, Quintais LT, Guerra-Assunção JA, Zhou Y, Gu Y, Yen J, Vogel JH, Eyre T, Redmond S, Banerjee R, Chi J, Fu B, Langley E, Maguire SF, Laird GK, Lloyd D, Kenyon E, Donaldson S, Sehra H, Almeida-King J, Loveland J, Trevanion S, Jones M, Quail M, Willey D, Hunt A, Burton J, Sims S, McLay K, Plumb B, Davis J, Clee C, Oliver K, Clark R, Riddle C, Elliot D, Eliott D, Threadgold G, Harden G, Ware D, Begum S, Mortimore B, Mortimer B, Kerry G, Heath P, Phillimore B, Tracey A, Corby N, Dunn M, Johnson C, Wood J, Clark S, Pelan S, Griffiths G, Smith M, Glithero R, Howden P, Barker N, Lloyd C, Stevens C, Harley J, Holt K, Panagiotidis G, Lovell J, Beasley H, Henderson C, Gordon D, Auger K, Wright D, Collins J, Raisen C, Dyer L, Leung K, Robertson L, Ambridge K, Leongamornlert D, McGuire S, Gilderthorp R, Griffiths C, Manthravadi D, Nichol S, Barker G, Whitehead S, Kay M, Brown J, Murnane C, Gray E, Humphries M, Sycamore N, Barker D, Saunders D, Wallis J, Babbage A, Hammond S, Mashreghi-Mohammadi M, Barr L, Martin S, Wray P, Ellington A, Matthews N, Ellwood M, Woodmansey R, Clark G, Cooper J, Cooper J, Tromans A, Grafham D, Skuce C, Pandian R, Andrews R, Harrison E, Kimberley A, Garnett J, Fosker N, Hall R, Garner P, Kelly D, Bird C, Palmer S, Gehring I, Berger A, Dooley CM, Ersan-Ürün Z, Eser C, Geiger H, Geisler M, Karotki L, Kirn A, Konantz J, Konantz M, Oberländer M, Rudolph-Geiger S, Teucke M, Lanz C, Raddatz G, Osoegawa K, Zhu B, Rapp A, Widaa S, Langford C, Yang F, Schuster SC, Carter NP, Harrow J, Ning Z, Herrero J, Searle SM, Enright A, Geisler R, Plasterk RH, Lee C, Westerfield M, de Jong PJ, Zon LI, Postlethwait JH, Nüsslein-Volhard C, Hubbard TJ, Roest Crollius H, Rogers J, Stemple DL. The zebrafish reference genome sequence and its relationship to the human genome. Nature 2013. PubMed
  • Ciarlo CA, Zon LI. A model for primary melanoma of the CNS implicates NRAS. 2013; 3:382-3. PubMed
  • Bowman TV, Zon LI. Ageing: Stem cells on a stress-busting diet. Nature 2013; 494:317-8. PubMed
  • Jing CB, Chen Y, Dong M, Peng XL, Jia XE, Gao L, Ma K, Deng M, Liu TX, Zon LI, Zhu J, Zhou Y, Zhou Y. Phospholipase C gamma-1 is required for granulocyte maturation in zebrafish. Dev Biol 2013; 374:24-31. PubMed
  • Cooney JD, Hildick-Smith GJ, Shafizadeh E, McBride PF, Carroll KJ, Anderson H, Shaw GC, Tamplin OJ, Branco DS, Dalton AJ, Shah DI, Wong C, Gallagher PG, Zon LI, North TE, Paw BH. Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Dev Biol 2012. PubMed
  • Bai X, Trowbridge JJ, Riley E, Lee JA, DiBiase A, Kaartinen VM, Orkin SH, Zon LI. TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes. Dev Biol 2012. PubMed
  • Kim PG, Albacker CE, Lu YF, Jang IH, Lim Y, Heffner GC, Arora N, Bowman TV, Lin MI, Lensch MW, De Los Angeles A, Zon LI, Loewer S, Daley GQ. Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial-to-hematopoietic transition. Proc Natl Acad Sci U S A 2013; 110:E141-50. PubMed
  • Paik EJ, Mahony S, White RM, Price EN, Dibiase A, Dorjsuren B, Mosimann C, Davidson AJ, Gifford D, Zon LI. A Cdx4-Sall4 regulatory module controls the transition from mesoderm formation to embryonic hematopoiesis. Stem Cell Reports 2013; 1:425-36. PubMed
  • McKinney-Freeman S, Cahan P, Li H, Lacadie SA, Huang HT, Curran M, Loewer S, Naveiras O, Kathrein KL, Konantz M, Langdon EM, Lengerke C, Zon LI, Collins JJ, Daley GQ. The transcriptional landscape of hematopoietic stem cell ontogeny. Cell Stem Cell 2012; 11:701-14. PubMed
  • Oubaha M, Lin MI, Margaron Y, Filion D, Price EN, Zon LI, Côté JF, Gratton JP. Formation of a PKCζ/β-catenin complex in endothelial cells promotes angiopoietin-1-induced collective directional migration and angiogenic sprouting. Blood 2012; 120:3371-81. PubMed
  • Lian CG, Xu Y, Ceol C, Wu F, Larson A, Dresser K, Xu W, Tan L, Hu Y, Zhan Q, Lee CW, Hu D, Lian BQ, Kleffel S, Yang Y, Neiswender J, Khorasani AJ, Fang R, Lezcano C, Duncan LM, Scolyer RA, Thompson JF, Kakavand H, Houvras Y, Zon LI, Mihm MC, Kaiser UB, Schatton T, Woda BA, Murphy GF, Shi YG. Loss of 5-hydroxymethylcytosine is an epigenetic hallmark of melanoma. Cell 2012; 150:1135-46. PubMed
  • Mione M, Zon LI. Cancer and inflammation: an aspirin a day keeps the cancer at bay. Curr Biol 2012; 22:R522-5. PubMed
  • Santoriello C, Zon LI. Hooked! Modeling human disease in zebrafish. J Clin Invest 2012; 122:2337-43. PubMed
  • Zon LI. A great match. Cell Stem Cell 2012; 10:638-9. PubMed
  • Jing L, Durand EM, Ezzio C, Pagliuca SM, Zon LI. In situ hybridization assay-based small molecule screening in zebrafish. Curr Protoc Chem Biol 2012. PubMed
  • Sorrells S, Carbonneau S, Harrington E, Chen AT, Hast B, Milash B, Pyati U, Major MB, Zhou Y, Zon LI, Stewart RA, Look AT, Jette C. Ccdc94 protects cells from ionizing radiation by inhibiting the expression of p53. PLoS Genet. 2012; 8:e1002922. PubMed
  • de Jong JL, Zon LI. Histocompatibility and hematopoietic transplantation in the zebrafish. Adv Hematol 2012; 2012:282318. PubMed
  • Gupta VA, Kawahara G, Myers JA, Chen AT, Hall TE, Manzini MC, Currie PD, Zhou Y, Zon LI, Kunkel LM, Beggs AH. A splice site mutation in laminin-α2 results in a severe muscular dystrophy and growth abnormalities in zebrafish. PLoS ONE 2012; 7:e43794. PubMed
  • Stachura DL, Svoboda O, Lau RP, Balla KM, Zon LI, Bartunek P, Traver D. Clonal analysis of hematopoietic progenitor cells in the zebrafish. Blood 2011. PubMed
  • Alexander MS, Kawahara G, Kho AT, Howell MH, Pusack TJ, Myers JA, Montanaro F, Zon LI, Guyon JR, Kunkel LM. Isolation and transcriptome analysis of adult zebrafish cells enriched for skeletal muscle progenitors. Muscle Nerve 2011. PubMed
  • Gupta V, Kawahara G, Gundry SR, Chen AT, Lencer WI, Zhou Y, Zon LI, Kunkel LM, Beggs AH. The zebrafish dag1 mutant: a novel genetic model for dystroglycanopathies. Hum Mol Genet 2011. PubMed
  • de Jong JL, Burns CE, Chen AT, Pugach E, Mayhall EA, Smith AC, Feldman HA, Zhou Y, Zon LI. Characterization of immune-matched hematopoietic transplantation in zebrafish. Blood 2011. PubMed
  • Bolli N, Payne EM, Rhodes J, Gjini E, Johnston AB, Guo F, Lee JS, Stewart RA, Kanki JP, Chen AT, Zhou Y, Zon LI, Look AT. cpsf1 is required for definitive HSC survival in zebrafish. Blood 2011. PubMed
  • Taylor AM, Zon LI. Modeling Diamond Blackfan anemia in the zebrafish. Semin Hematol 2011; 48:81-8. PubMed
  • White RM, Cech J, Ratanasirintrawoot S, Lin CY, Rahl PB, Burke CJ, Langdon E, Tomlinson ML, Mosher J, Kaufman C, Chen F, Long HK, Kramer M, Datta S, Neuberg D, Granter S, Young RA, Morrison S, Wheeler GN, Zon LI. DHODH modulates transcriptional elongation in the neural crest and melanoma. Nature 2011; 471:518-22. PubMed
  • Ceol CJ, Houvras Y, Jane-Valbuena J, Bilodeau S, Orlando DA, Battisti V, Fritsch L, Lin WM, Hollmann TJ, Ferré F, Bourque C, Burke CJ, Turner L, Uong A, Johnson LA, Beroukhim R, Mermel CH, Loda M, Ait-Si-Ali S, Garraway LA, Young RA, Zon LI. The histone methyltransferase SETDB1 is recurrently amplified in melanoma and accelerates its onset. Nature 2011; 471:513-7. PubMed
  • Lacadie SA, Zon LI. The ERGonomics of hematopoietic stem cell self-renewal. Genes Dev 2011; 25:289-93. PubMed
  • Mosimann C, Kaufman CK, Li P, Pugach EK, Tamplin OJ, Zon LI. Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish. Development 2010; 138:169-77. PubMed
  • Durand EM, Zon LI. Stem cells: The blood balance. Nature 2010; 468:644-5. PubMed
  • Feng H, Stachura DL, White RM, Gutierrez A, Zhang L, Sanda T, Jette CA, Testa JR, Neuberg DS, Langenau DM, Kutok JL, Zon LI, Traver D, Fleming MD, Kanki JP, Look AT. T-lymphoblastic lymphoma cells express high levels of BCL2, S1P1, and ICAM1, leading to a blockade of tumor cell intravasation. Cancer Cell 2010; 18:353-66. PubMed
  • North TE, Babu IR, Vedder LM, Lord AM, Wishnok JS, Tannenbaum SR, Zon LI, Goessling W. PGE2-regulated wnt signaling and N-acetylcysteine are synergistically hepatoprotective in zebrafish acetaminophen injury. Proc Natl Acad Sci U S A 2010; 107:17315-20. PubMed
  • Koo S, Huntly B, Wang Y, Chen J, Brumme K, Ball B, McKinney-Freeman S, Yabuuchi A, Scholl C, Bansal D, Zon L, Froehling S, Daley G, Gilliland G, Mercher T. Cdx4 is dispensable for murine adult hematopoietic stem cells but promotes MLL-AF9-mediated leukemogenesis. Haematologica 2010; 95:1642-50. PubMed
  • Tamplin OJ, Zon LI. Fishing at the cellular level. Nat Methods 2010; 7:600-1. PubMed
  • Storer NY, Zon LI. Zebrafish models of p53 functions. Cold Spring Harb Perspect Biol 2010; 2:a001123. PubMed
  • de Jong JL, Davidson AJ, Wang Y, Palis J, Opara P, Pugach E, Daley GQ, Zon LI. Interaction of retinoic acid and scl controls primitive blood development. Blood 2010; 116:201-9. PubMed
  • Tamplin OJ, Zon LI. Blood flow: metalloproteases cut loose in primitive erythrocytes. Curr Biol 2010; 20:R561-2. PubMed
  • Bai X, Kim J, Yang Z, Jurynec MJ, Akie TE, Lee J, LeBlanc J, Sessa A, Jiang H, DiBiase A, Zhou Y, Grunwald DJ, Lin S, Cantor AB, Orkin SH, Zon LI. TIF1gamma controls erythroid cell fate by regulating transcription elongation. Cell 2010; 142:133-43. PubMed
  • Durand EM, Zon LI. Newly emerging roles for prostaglandin E2 regulation of hematopoiesis and hematopoietic stem cell engraftment. Curr Opin Hematol 2010; 17:308-12. PubMed
  • Smith AC, Raimondi AR, Salthouse CD, Ignatius MS, Blackburn JS, Mizgirev IV, Storer NY, de Jong JL, Chen AT, Zhou Y, Revskoy S, Zon LI, Langenau DM. High-throughput cell transplantation establishes that tumor-initiating cells are abundant in zebrafish T-cell acute lymphoblastic leukemia. Blood 2010; 115:3296-303. PubMed
  • Wang Y, Krivtsov AV, Sinha AU, North TE, Goessling W, Feng Z, Zon LI, Armstrong SA. The Wnt/beta-catenin pathway is required for the development of leukemia stem cells in AML. Science 2010; 327:1650-3. PubMed
  • Li P, Zon LI. Resolving the controversy about N-cadherin and hematopoietic stem cells. Cell Stem Cell 2010; 6:199-202. PubMed
  • Paik EJ, de Jong JL, Pugach E, Opara P, Zon LI. A chemical genetic screen in zebrafish for pathways interacting with cdx4 in primitive hematopoiesis. Zebrafish 2010; 7:61-8. PubMed
  • Zon LI, Peterson R. The new age of chemical screening in zebrafish. Zebrafish 2010; 7:1. PubMed
  • Bowman TV, Zon LI. Swimming into the future of drug discovery: in vivo chemical screens in zebrafish. ACS Chem Biol 2010; 5:159-61. PubMed
  • Uong A, Zon LI. Melanocytes in development and cancer. J Cell Physiol 2009; 222:38-41. PubMed
  • Paik EJ, Zon LI. Hematopoietic development in the zebrafish. Int J Dev Biol 2010; 54:1127-37. PubMed
  • Trompouki E, Zon LI. Small molecule screen in zebrafish and HSC expansion. Methods Mol Biol 2010; 636:301-16. PubMed
  • Taylor AM, Zon LI. Zebrafish tumor assays: the state of transplantation. Zebrafish 2010; 6:339-46. PubMed
  • Dovey M, White RM, Zon LI. Oncogenic NRAS cooperates with p53 loss to generate melanoma in zebrafish. Zebrafish 2010; 6:397-404. PubMed
  • Chen AT, Zon LI. Zebrafish blood stem cells. J Cell Biochem 2009; 108:35-42. PubMed
  • Stachura DL,Reyes JR,Bartunek P,Paw BH,Zon LI,Traver D. Zebrafish kidney stromal cell lines support multilineage hematopoiesis. Blood 2009; 114:279-89. PubMed
  • Dovey M,Patton EE,Bowman T,North T,Goessling W,Zhou Y,Zon LI. Topoisomerase II alpha is required for embryonic development and liver regeneration in zebrafish. Mol Cell Biol 2009; 29:3746-53. PubMed
  • Burns CE,Galloway JL,Smith AC,Keefe MD,Cashman TJ,Paik EJ,Mayhall EA,Amsterdam AH,Zon LI. A genetic screen in zebrafish defines a hierarchical network of pathways required for hematopoietic stem cell emergence. Blood 2009; 113:5776-82. PubMed
  • North TE,Goessling W,Peeters M,Li P,Ceol C,Lord AM,Weber GJ,Harris J,Cutting CC,Huang P,Dzierzak E,Zon LI. Hematopoietic stem cell development is dependent on blood flow. Cell 2009; 137:736-48. PubMed
  • Goessling W,North TE,Loewer S,Lord AM,Lee S,Stoick-Cooper CL,Weidinger G,Puder M,Daley GQ,Moon RT,Zon LI. Genetic interaction of PGE2 and Wnt signaling regulates developmental specification of stem cells and regeneration. Cell 2009; 136:1136-47. PubMed
  • Fraenkel PG,Gibert Y,Holzheimer JL,Lattanzi VJ,Burnett SF,Dooley KA,Wingert RA,Zon LI. Transferrin-a modulates hepcidin expression in zebrafish embryos. Blood 2008; 113:2843-50. PubMed
  • de Jong-Curtain TA,Parslow AC,Trotter AJ,Hall NE,Verkade H,Tabone T,Christie EL,Crowhurst MO,Layton JE,Shepherd IT,Nixon SJ,Parton RG,Zon LI,Stainier DY,Lieschke GJ,Heath JK. Abnormal nuclear pore formation triggers apoptosis in the intestinal epithelium of elys-deficient zebrafish. Gastroenterology 2008; 136:902-11. PubMed
  • Freeman JL,Ceol C,Feng H,Langenau DM,Belair C,Stern HM,Song A,Paw BH,Look AT,Zhou Y,Zon LI,Lee C. Construction and application of a zebrafish array comparative genomic hybridization platform. Genes Chromosomes Cancer 2008; 48:155-70. PubMed
  • Dovey MC, Zon LI. Defining cancer stem cells by xenotransplantation in zebrafish. Methods Mol Biol 2009; 568:1-5. PubMed
  • Sessa AK,White R,Houvras Y,Burke C,Pugach E,Baker B,Gilbert R,Thomas Look A,Zon LI. The effect of a depth gradient on the mating behavior, oviposition site preference, and embryo production in the zebrafish, Danio rerio. Zebrafish 2009; 5:335-9. PubMed
  • Ceol CJ,Houvras Y,White RM,Zon LI. Melanoma biology and the promise of zebrafish. Zebrafish 2009; 5:247-55. PubMed
  • Flores MV,Hall CJ,Davidson AJ,Singh PP,Mahagaonkar AA,Zon LI,Crosier KE,Crosier PS. Intestinal differentiation in zebrafish requires Cdx1b, a functional equivalent of mammalian Cdx2. Gastroenterology 2008; 135:1665-75. PubMed
  • Wang L,Sewell WF,Kim SD,Shin JT,MacRae CA,Zon LI,Seidman JG,Seidman CE. Eya4 regulation of Na+/K+-ATPase is required for sensory system development in zebrafish. Development 2008; 135:3425-34. PubMed
  • White RM,Zon LI. Melanocytes in development, regeneration, and cancer. Cell Stem Cell 2008; 3:242-52. PubMed
  • Dooley KA,Fraenkel PG,Langer NB,Schmid B,Davidson AJ,Weber G,Chiang K,Foott H,Dwyer C,Wingert RA,Zhou Y,Paw BH,Zon LI. montalcino, A zebrafish model for variegate porphyria. Exp Hematol 2008; 36:1132-42. PubMed
  • Trede NS,Ota T,Kawasaki H,Paw BH,Katz T,Demarest B,Hutchinson S,Zhou Y,Hersey C,Zapata A,Amemiya CT,Zon LI. Zebrafish mutants with disrupted early T-cell and thymus development identified in early pressure screen. Dev Dyn 2008; 237:2575-84. PubMed
  • Goessling W, North TE, Lord AM, Ceol C, Lee S, Weidinger G, Bourque C, Strijbosch R, Haramis AP, Puder M, Clevers H, Moon RT, Zon LI. APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development. Dev Biol 2008; 320:161-74. PubMed
  • Zon LI. Self-renewal and differentiation at Cell Stem Cell. Cell Stem Cell 2008; 2:510. PubMed
  • Zon LI. Intrinsic and extrinsic control of haematopoietic stem-cell self-renewal. Nature 2008; 453:306-13. PubMed
  • Galloway JL, Wingert RA, Thisse C, Thisse B, Zon LI. Combinatorial regulation of novel erythroid gene expression in zebrafish. Exp Hematol 2008; 36:424-32. PubMed
  • Burket CT, Montgomery JE, Thummel R, Kassen SC, LaFave MC, Langenau DM, Zon LI, Hyde DR. Generation and characterization of transgenic zebrafish lines using different ubiquitous promoters. Transgenic Res 2007; 17:265-79. PubMed
  • Deutsch EW, Ball CA, Berman JJ, Bova GS, Brazma A, Bumgarner RE, Campbell D, Causton HC, Christiansen JH, Daian F, Dauga D, Davidson DR, Gimenez G, Goo YA, Grimmond S, Henrich T, Herrmann BG, Johnson MH, Korb M, Mills JC, Oudes AJ, Parkinson HE, Pascal LE, Pollet N, Quackenbush J, Ramialison M, Ringwald M, Salgado D, Sansone SA, Sherlock G, Stoeckert CJ Jr, Swedlow J, Taylor RC, Walashek L, Warford A, Wilkinson DG, Zhou Y, Zon LI, Liu AY, True LD. Minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). Nat Biotechnol 2008; 26:305-12. PubMed
  • Orkin SH, Zon LI. SnapShot: hematopoiesis. Cell 2008; 132:712. PubMed
  • Orkin SH, Zon LI. Hematopoiesis: an evolving paradigm for stem cell biology. Cell 2008; 132:631-44. PubMed
  • White RM, Sessa A, Burke C, Bowman T, LeBlanc J, Ceol C, Bourque C, Dovey M, Goessling W, Burns CE, Zon LI. Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell 2008; 2:183-9. PubMed
  • Lengerke C, Schmitt S, Bowman TV, Jang IH, Maouche-Chretien L, McKinney-Freeman S, Davidson AJ, Hammerschmidt M, Rentzsch F, Green JB, Zon LI, Daley GQ. BMP and Wnt specify hematopoietic fate by activation of the Cdx-Hox pathway. Cell Stem Cell 2008; 2:72-82. PubMed
  • Imamura S,Uchiyama J,Koshimizu E,Hanai J,Raftopoulou C,Murphey RD,Bayliss PE,Imai Y,Burns CE,Masutomi K,Gagos S,Zon LI,Roberts TM,Kishi S. A non-canonical function of zebrafish telomerase reverse transcriptase is required for developmental hematopoiesis. PLoS ONE 2008; 3:e3364. PubMed
  • Lord AM, North TE, Zon LI. Prostaglandin E2: making more of your marrow. Cell Cycle 2007; 6:3054-7. PubMed
  • Bahary N, Goishi K, Stuckenholz C, Weber G, Leblanc J, Schafer CA, Berman SS, Klagsbrun M, Zon LI. Duplicate VegfA genes and orthologues of the KDR receptor tyrosine kinase family mediate vascular development in the zebrafish. Blood 2007; 110:3627-36. PubMed
  • Ceol CJ, Pellman D, Zon LI. APC and colon cancer: two hits for one. Nat Med 2007; 13:1286-7. PubMed
  • Paffett-Lugassy N, Hsia N, Fraenkel PG, Paw B, Leshinsky I, Barut B, Bahary N, Caro J, Handin R, Zon LI. Functional conservation of erythropoietin signaling in zebrafish. Blood 2007; 110:2718-26. PubMed
  • Lensch MW, Schlaeger TM, Zon LI, Daley GQ. Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimera. Cell Stem Cell 2008; 1:253-8. PubMed
  • Pfaff KL, Straub CT, Chiang K, Bear DM, Zhou Y, Zon LI. The zebra fish cassiopeia mutant reveals that SIL is required for mitotic spindle organization. Mol Cell Biol 2007; 27:5887-97. PubMed
  • Goessling W, North TE, Zon LI. Ultrasound biomicroscopy permits in vivo characterization of zebrafish liver tumors. Nat Methods 2007; 4:551-3. PubMed
  • North TE, Goessling W, Walkley CR, Lengerke C, Kopani KR, Lord AM, Weber GJ, Bowman TV, Jang IH, Grosser T, Fitzgerald GA, Daley GQ, Orkin SH, Zon LI. Prostaglandin E2 regulates vertebrate haematopoietic stem cell homeostasis. Nature 2007; 447:1007-11. PubMed
  • Goessling W, North TE, Zon LI. New waves of discovery: modeling cancer in zebrafish. J Clin Oncol 2007; 25:2473-9. PubMed
  • Zon LI. Cell stem cell. Cell Stem Cell 2008; 1:7. PubMed
  • Langenau DM, Keefe MD, Storer NY, Guyon JR, Kutok JL, Le X, Goessling W, Neuberg DS, Kunkel LM, Zon LI. Effects of RAS on the genesis of embryonal rhabdomyosarcoma. Genes Dev 2007; 21:1382-1395. PubMed
  • Le X, Langenau DM, Keefe MD, Kutok JL, Neuberg DS, Zon LI. Heat shock-inducible Cre/Lox approaches to induce diverse types of tumors and hyperplasia in transgenic zebrafish. Proc Natl Acad Sci U S A 2007; 104:9410-5. PubMed
  • Trede NS, Medenbach J, Damianov A, Hung LH, Weber GJ, Paw BH, Zhou Y, Hersey C, Zapata A, Keefe M, Barut BA, Stuart AB, Katz T, Amemiya CT, Zon LI, Bindereif A. Network of coregulated spliceosome components revealed by zebrafish mutant in recycling factor p110. Proc Natl Acad Sci U S A 2007; 104:6608-13. PubMed
  • Hultman KA, Bahary N, Zon LI, Johnson SL. Gene Duplication of the zebrafish kit ligand and partitioning of melanocyte development functions to kit ligand a. PLoS Genet 2007; 3:e17. PubMed
  • Shepard JL, Amatruda JF, Finkelstein D, Ziai J, Finley KR, Stern HM, Chiang K, Hersey C, Barut B, Freeman JL, Lee C, Glickman JN, Kutok JL, Aster JC, Zon LI. A mutation in separase causes genome instability and increased susceptibility to epithelial cancer. Genes Dev 2007; 21:55-9. PubMed
  • Steffen LS, Guyon JR, Vogel ED, Beltre R, Pusack TJ, Zhou Y, Zon LI, Kunkel LM. Zebrafish orthologs of human muscular dystrophy genes. BMC Genomics 2007; 8:79. PubMed
  • Bansal D, Scholl C, Fr. Cdx4 dysregulates Hox gene expression and generates acute myeloid leukemia alone and in cooperation with Meis1a in a murine model. Proc Natl Acad Sci U S A 2006; 103:16924-9. PubMed
  • Hu G, Tang J, Zhang B, Lin Y, Hanai J, Galloway J, Bedell V, Bahary N, Han Z, Ramchandran R, Thisse B, Thisse C, Zon LI, Sukhatme VP. A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro. J Cell Sci 2006; 119:4117-26. PubMed
  • Murphey RD, Stern HM, Straub CT, Zon LI. A chemical genetic screen for cell cycle inhibitors in zebrafish embryos. Chem Biol Drug Des 2006; 68:213-9. PubMed
  • Murphey RD, Zon LI. Small molecule screening in the zebrafish. Methods 2006; 39:255-61. PubMed
  • Gupta S, Zhu H, Zon LI, Evans T. BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis. Development 2006; 133:2177-87. PubMed
  • Davidson AJ, Zon LI. The caudal-related homeobox genes cdx1a and cdx4 act redundantly to regulate hox gene expression and the formation of putative hematopoietic stem cells during zebrafish embryogenesis. Dev Biol 2006; 292:506-18. PubMed
  • Shaw GC, Cope JJ, Li L, Corson K, Hersey C, Ackermann GE, Gwynn B, Lambert AJ, Wingert RA, Traver D, Trede NS, Barut BA, Zhou Y, Minet E, Donovan A, Brownlie A, Balzan R, Weiss MJ, Peters LL, Kaplan J, Zon LI, Paw BH. Mitoferrin is essential for erythroid iron assimilation. Nature 2006; 440:96-100. PubMed
  • Cutler C, Multani P, Robbins D, Kim HT, Le T, Hoggatt J, Pelus LM, Desponts C, Chen YB, Rezner B, Armand P, Koreth J, Glotzbecker B, Ho VT, Alyea E, Isom M, Kao G, Armant M, Silberstein L, Hu P, Soiffer RJ, Scadden DT, Ritz J, Goessling W, North TE, Mendlein J, Ballen K, Zon LI, Antin JH, Shoemaker DD. Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation Blood 2013.
  • Xu C, Tabebordbar M, Lovino S, Ciarlo C, Liu J, Castiglioni A, Price E, Kahn RC, Wagers AJ, and Zon LI. A zebrafish embryo culture system defines factors that promote vertebrate myogenesis across species Cell 2013.
  • Huang H-T, Kathrein KL, Barton A, Gitlin Z, Huang Y-H, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, and Zon LI. A network of epigenetic regulators guide developmental hematopoiesis in vivo Nat Cell Biol 2013.
Hide