Photo of Nathalie Y. R. Agar,  PhD

Nathalie Y. R. Agar, PhD

Brigham And Women's Hospital

Brigham And Women's Hospital
Phone: (617) 525-7374
Fax: (617) 264-6316


nagar@rics.bwh.harvard.edu

Nathalie Y. R. Agar, PhD

Brigham And Women's Hospital

EDUCATIONAL TITLES

  • Assistant Professor, Radiology, Harvard Medical School
  • Assistant Professor, Neurosurgery, Harvard Medical School
  • Research Scientist, Neurosurgery, Brigham And Women's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

The Agar Laboratory develops and validates mass spectrometry imaging methodologies, and applies them to study neuro-oncology and to potentially guide the care of patients affected by brain cancer.

Imaging of drug distribution and metabolism

The limited penetrance of molecules through the blood-brain barrier induces additional complexity in the process of developing drugs targeting the central nervous system (CNS). Using MALDI mass spectrometry imaging, our laboratory in collaboration with Dr. Charles Stiles (Dept. of Cancer Biology, DFCI) can now rapidly assess the distribution and metabolism of drugs in the CNS of animal models without the use of molecular or radioactive probes.

Intraoperative stereotactic mass spectrometry imaging

In an effort to guide neurosurgical decision-making, our laboratory is integrating the use of mass spectrometry as an intraoperative molecular imaging approach. This transdisciplinary work is carried in close collaboration with groups from the departments of Neurosurgery, Radiology, and Pathology at BWH, as well as groups from Purdue University, and the Georgia Institute of Technology. The integrated platform should eventually provide real-time molecular assessment of tumor margins and diagnostic information to maximize tumor resection, while preserving surrounding healthy brain tissue.

 

Publications

Powered by Harvard Catalyst
  • Du Z, Abedalthagafi M, Aizer AA, McHenry AR, Sun HH, Bray MA, Viramontes O, Machaidze R, Brastianos PK, Reardon DA, Dunn IF, Freeman GJ, Ligon KL, Carpenter AE, Alexander BM, Agar NY, Rodig SJ, Bradshaw EM, Santagata S. Increased expression of the immune modulatory molecule PD-L1 (CD274) in anaplastic meningioma. 2015; 6:4704-16. PubMed
  • Calligaris D, Feldman DR, Norton I, Brastianos PK, Dunn IF, Santagata S, Agar NY. Molecular typing of Meningiomas by Desorption Electrospray Ionization Mass Spectrometry Imaging for Surgical Decision-Making. 2015; 377:690-698. PubMed
  • Pokorny JL, Calligaris D, Gupta SK, Iyekegbe DO, Mueller D, Bakken KK, Carlson BL, Schroeder MA, Evans DL, Lou Z, Decker PA, Eckel-Passow JE, Pucci V, Ma B, Shumway SD, Elmquist WF, Agar NY, Sarkaria JN. The Efficacy of the Wee1 Inhibitor MK-1775 Combined with Temozolomide Is Limited by Heterogeneous Distribution across the Blood-Brain Barrier in Glioblastoma. Clin Cancer Res 2015. PubMed
  • Calligaris D, Caragacianu D, Liu X, Norton I, Thompson CJ, Richardson AL, Golshan M, Easterling ML, Santagata S, Dillon DA, Jolesz FA, Agar NY. Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis. Proc Natl Acad Sci U S A 2014. PubMed
  • Tempany CM, Jayender J, Kapur T, Bueno R, Golby A, Agar N, Jolesz FA. Multimodal imaging for improved diagnosis and treatment of cancers. Cancer 2014. PubMed
  • Santagata S, Eberlin LS, Norton I, Calligaris D, Feldman DR, Ide JL, Liu X, Wiley JS, Vestal ML, Ramkissoon SH, Orringer DA, Gill KK, Dunn IF, Dias-Santagata D, Ligon KL, Jolesz FA, Golby AJ, Cooks RG, Agar NY. Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery. Proc Natl Acad Sci U S A 2014; 111:11121-6. PubMed
  • Auclair JR, Salisbury JP, Johnson JL, Petsko GA, Ringe D, Bosco DA, Agar NY, Santagata S, Durham HD, Agar JN. Artifacts to avoid while taking advantage of top-down mass spectrometry based detection of protein S-thiolation. Proteomics 2014. PubMed
  • Calligaris D, Norton I, Feldman DR, Ide JL, Dunn IF, Eberlin LS, Cooks RG, Jolesz FA, Golby AJ, Santagata S, Agar NY. Mass spectrometry imaging as a tool for surgical decision-making. J Mass Spectrom 2013; 48:1178-87. PubMed
  • Ji M, Orringer DA, Freudiger CW, Ramkissoon S, Liu X, Lau D, Golby AJ, Norton I, Hayashi M, Agar NY, Young GS, Spino C, Santagata S, Camelo-Piragua S, Ligon KL, Sagher O, Xie XS. Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy. Sci Transl Med 2013; 5:201ra119. PubMed
  • Eberlin LS, Norton I, Orringer D, Dunn IF, Liu X, Ide JL, Jarmusch AK, Ligon KL, Jolesz FA, Golby AJ, Santagata S, Agar NY, Cooks RG. Ambient mass spectrometry for the intraoperative molecular diagnosis of human brain tumors. Proc Natl Acad Sci U S A 2013; 110:1611-6. PubMed
  • Liu X, Ide JL, Norton I, Marchionni MA, Ebling MC, Wang LY, Davis E, Sauvageot CM, Kesari S, Kellersberger KA, Easterling ML, Santagata S, Stuart DD, Alberta J, Agar JN, Stiles CD, Agar NY. Molecular imaging of drug transit through the blood-brain barrier with MALDI mass spectrometry imaging. Sci Rep 2013; 3:2859. PubMed
  • Rodriguez N, Yang J, Hasselblatt K, Liu S, Zhou Y, Rauh-Hain JA, Ng SK, Choi PW, Fong WP, Agar NY, Welch WR, Berkowitz RS, Ng SW. Casein kinase I epsilon interacts with mitochondrial proteins for the growth and survival of human ovarian cancer cells. EMBO Mol Med 2012; 4:952-63. PubMed
  • Gholami B, Norton I, Tannenbaum AR, Agar NY. Recursive feature elimination for brain tumor classification using desorption electrospray ionization mass spectrometry imaging. Conf Proc IEEE Eng Med Biol Soc 2013; 2012:5258-61. PubMed
  • Agar NY, Golby AJ, Ligon KL, Norton I, Mohan V, Wiseman JM, Tannenbaum A, Jolesz FA. Development of Stereotactic Mass Spectrometry for Brain Tumor Surgery. Neurosurgery 2011. PubMed
  • Eberlin LS, Dill AL, Golby AJ, Ligon KL, Wiseman JM, Cooks RG, Agar NY. Discrimination of human astrocytoma subtypes by lipid analysis using desorption electrospray ionization imaging mass spectrometry. Angew Chem Int Ed Engl 2010; 49:5953-6. PubMed
  • Karabacak NM, Easterling ML, Agar NY, Agar JN. Transformative effects of higher magnetic field in Fourier transform ion cyclotron resonance mass spectrometry. J. Am. Soc. Mass Spectrom. 2010; 21:1218-22. PubMed
  • Agar NY, Malcolm JG, Mohan V, Yang HW, Johnson MD, Tannenbaum A, Agar JN, Black PM. Imaging of Meningioma Progression by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry. Anal Chem 2010; 82:2621-5. PubMed
  • Agar NY, Kowalski JM, Kowalski PJ, Wong JH, Agar JN. Tissue preparation for the in situ MALDI MS imaging of proteins, lipids, and small molecules at cellular resolution. Methods Mol Biol 2010; 656:415-31. PubMed
  • Wang Q, Johnson JL, Agar NY, Agar JN. Protein aggregation and protein instability govern familial amyotrophic lateral sclerosis patient survival. PLoS Biol. 2008; 6:e170. PubMed
  • Agar NY, Yang HW, Carroll RS, Black PM, Agar JN. Matrix solution fixation: histology-compatible tissue preparation for MALDI mass spectrometry imaging. Anal Chem 2007; 79:7416-23. PubMed
  • Werbowetski-Ogilvie TE, Seyed Sadr M, Jabado N, Angers-Loustau A, Agar NY, Wu J, Bjerkvig R, Antel JP, Faury D, Rao Y, Del Maestro RF. Inhibition of medulloblastoma cell invasion by Slit. Oncogene 2006; 25:5103-12. PubMed
  • Werbowetski-Ogilvie TE, Agar NY, Waldkircher de Oliveira RM, Faury D, Antel JP, Jabado N, Del Maestro RF. Isolation of a natural inhibitor of human malignant glial cell invasion: inter alpha-trypsin inhibitor heavy chain 2. Cancer Res 2006; 66:1464-72. PubMed
Hide