Photo of Norbert Perrimon,  PhD

Norbert Perrimon, PhD

Harvard Medical School

Harvard Medical School
Phone: (617) 432-7672
Fax: (617) 432-7688


perrimon@receptor.med.harvard.edu

Norbert Perrimon, PhD

Harvard Medical School

EDUCATIONAL TITLES

  • James Stillman Professor of Developmental Biology, Genetics, Harvard Medical School

DF/HCC PROGRAM AFFILIATION

Research Abstract

Our laboratory uses Drosophila as a model system to characterize evolutionary conserved signaling transduction pathways. Our current studies focus on the analysis of Wnt, EGF Receptor, and JAK/STAT signaling transduction pathways. In addition, other projects in the lab focus on the characterization of signaling pathways that regulate the establishment of polarity and cytoskeletal organization.

Publications

Powered by Harvard Catalyst
  • Kwon Y, Song W, Droujinine IA, Hu Y, Asara JM, Perrimon N. Systemic Organ Wasting Induced by Localized Expression of the Secreted Insulin/IGF Antagonist ImpL2. Dev Cell 2015; 33:36-46. PubMed
  • Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM. Highly efficient Cas9-mediated transcriptional programming. Nat Methods 2015; 12:326-8. PubMed
  • Sopko R, Lin YB, Makhijani K, Alexander B, Perrimon N, Brückner K. A systems-level interrogation identifies regulators of Drosophila blood cell number and survival. PLoS Genet. 2015; 11:e1005056. PubMed
  • Leshchiner ES, Parkhitko A, Bird GH, Luccarelli J, Bellairs JA, Escudero S, Opoku-Nsiah K, Godes M, Perrimon N, Walensky LD. Direct inhibition of oncogenic KRAS by hydrocarbon-stapled SOS1 helices. Proc Natl Acad Sci U S A 2015; 112:1761-6. PubMed
  • Zirin J, Nieuwenhuis J, Samsonova A, Tao R, Perrimon N. Regulators of autophagosome formation in Drosophila muscles. PLoS Genet. 2015; 11:e1005006. PubMed
  • Perrimon N. Drosophila developmental biology methods. Methods 2014; 68:1. PubMed
  • Demontis F, Patel VK, Swindell WR, Perrimon N. Intertissue Control of the Nucleolus via a Myokine-Dependent Longevity Pathway. Cell Rep 2014; 7:1481-94. PubMed
  • Kim K, Vinayagam A, Perrimon N. A Rapid Genome-wide MicroRNA Screen Identifies miR-14 as a Modulator of Hedgehog Signaling. Cell Rep 2014. PubMed
  • Zhou R, Mohr S, Hannon GJ, Perrimon N. Inducing RNAi in Drosophila cells by soaking with dsRNA. Cold Spring Harb Protoc 2014. PubMed
  • Markstein M, Dettorre S, Cho J, Neumüller RA, Craig-Müller S, Perrimon N. Systematic screen of chemotherapeutics in Drosophila stem cell tumors. Proc Natl Acad Sci U S A 2014; 111:4530-5. PubMed
  • Mohr SE, Hu Y, Kim K, Housden BE, Perrimon N. Resources for Functional Genomics Studies in Drosophila melanogaster. Genetics 2014. PubMed
  • Brown JB, Boley N, Eisman R, May GE, Stoiber MH, Duff MO, Booth BW, Wen J, Park S, Suzuki AM, Wan KH, Yu C, Zhang D, Carlson JW, Cherbas L, Eads BD, Miller D, Mockaitis K, Roberts J, Davis CA, Frise E, Hammonds AS, Olson S, Shenker S, Sturgill D, Samsonova AA, Weiszmann R, Robinson G, Hernandez J, Andrews J, Bickel PJ, Carninci P, Cherbas P, Gingeras TR, Hoskins RA, Kaufman TC, Lai EC, Oliver B, Perrimon N, Graveley BR, Celniker SE. Diversity and dynamics of the Drosophila transcriptome. Nature 2014. PubMed
  • Owusu-Ansah E, Perrimon N. Modeling metabolic homeostasis and nutrient sensing in Drosophila: implications for aging and metabolic diseases. Dis Model Mech 2014; 7:343-50. PubMed
  • Yan D, Neumüller RA, Buckner M, Ayers K, Li H, Hu Y, Yang-Zhou D, Pan L, Wang X, Kelley C, Vinayagam A, Binari R, Randklev S, Perkins LA, Xie T, Cooley L, Perrimon N. A regulatory network of Drosophila germline stem cell self-renewal. Dev Cell 2014; 28:459-73. PubMed
  • Piccirillo R, Demontis F, Perrimon N, Goldberg AL. Mechanisms of muscle growth and atrophy in mammals and Drosophila. Dev Dyn 2014; 243:201-15. PubMed
  • Doupé DP, Perrimon N. Visualizing and manipulating temporal signaling dynamics with fluorescence-based tools. Sci Signal 2014; 7:re1. PubMed
  • Vinayagam A, Zirin J, Roesel C, Hu Y, Yilmazel B, Samsonova AA, Neumüller RA, Mohr SE, Perrimon N. Integrating protein-protein interaction networks with phenotypes reveals signs of interactions. Nat Methods 2013; 11:94-9. PubMed
  • Demontis F, Piccirillo R, Goldberg AL, Perrimon N. The influence of skeletal muscle on systemic aging and lifespan. Aging Cell 2013; 12:943-9. PubMed
  • Ren X, Sun J, Housden BE, Hu Y, Roesel C, Lin S, Liu LP, Yang Z, Mao D, Sun L, Wu Q, Ji JY, Xi J, Mohr SE, Xu J, Perrimon N, Ni JQ. Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9. Proc Natl Acad Sci U S A 2013; 110:19012-7. PubMed
  • Kwon Y, Vinayagam A, Sun X, Dephoure N, Gygi SP, Hong P, Perrimon N. The Hippo signaling pathway interactome. Science 2013; 342:737-40. PubMed
  • Sun X, Hong P, Kulkarni M, Kwon Y, Perrimon N. PPIRank - an advanced method for ranking protein-protein interations in TAP/MS data. Proteome Sci 2014; 11:S16. PubMed
  • Owusu-Ansah E, Song W, Perrimon N. Muscle mitohormesis promotes longevity via systemic repression of insulin signaling. Cell 2013; 155:699-712. PubMed
  • Xiong XP, Kurthkoti K, Chang KY, Lichinchi G, De N, Schneemann A, MacRae IJ, Rana TM, Perrimon N, Zhou R. Core small nuclear ribonucleoprotein particle splicing factor SmD1 modulates RNA interference in Drosophila. Proc Natl Acad Sci U S A 2013; 110:16520-5. PubMed
  • Hu Y, Roesel C, Flockhart I, Perkins L, Perrimon N, Mohr SE. UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents. Genetics 2013; 195:37-45. PubMed
  • Hu Y, Sopko R, Foos M, Kelley C, Flockhart I, Ammeux N, Wang X, Perkins L, Perrimon N, Mohr SE. FlyPrimerBank: an online database for Drosophila melanogaster gene expression analysis and knockdown evaluation of RNAi reagents. 2013; 3:1607-16. PubMed
  • Neumüller RA, Gross T, Samsonova AA, Vinayagam A, Buckner M, Founk K, Hu Y, Sharifpoor S, Rosebrock AP, Andrews B, Winston F, Perrimon N. Conserved regulators of nucleolar size revealed by global phenotypic analyses. Sci Signal 2013; 6:ra70. PubMed
  • Yin Z, Sadok A, Sailem H, McCarthy A, Xia X, Li F, Garcia MA, Evans L, Barr AR, Perrimon N, Marshall CJ, Wong ST, Bakal C. A screen for morphological complexity identifies regulators of switch-like transitions between discrete cell shapes. Nat Cell Biol 2013; 15:860-71. PubMed
  • Sopko R, Perrimon N. Receptor tyrosine kinases in Drosophila development. Cold Spring Harb Perspect Biol 2013. PubMed
  • Roti G, Carlton A, Ross KN, Markstein M, Pajcini K, Su AH, Perrimon N, Pear WS, Kung AL, Blacklow SC, Aster JC, Stegmaier K. Complementary genomic screens identify SERCA as a therapeutic target in NOTCH1 mutated cancer. Cancer Cell 2013; 23:390-405. PubMed
  • Staller MV, Yan D, Randklev S, Bragdon MD, Wunderlich ZB, Tao R, Perkins LA, Depace AH, Perrimon N. Depleting Gene Activities in Early Drosophila Embryos with the "maternal-Gal4 - shRNA" System. Genetics 2012. PubMed
  • Stender JD, Pascual G, Liu W, Kaikkonen MU, Do K, Spann NJ, Boutros M, Perrimon N, Rosenfeld MG, Glass CK. Control of Proinflammatory Gene Programs by Regulated Trimethylation and Demethylation of Histone H4K20. Mol Cell 2012; 48:28-38. PubMed
  • Rajan A, Perrimon N. Drosophila cytokine unpaired 2 regulates physiological homeostasis by remotely controlling insulin secretion. Cell 2012; 151:123-37. PubMed
  • Hosur R, Peng J, Vinayagam A, Stelzl U, Xu J, Perrimon N, Bienkowska J, Berger B. Coev2Net: a computational framework for boosting confidence in high-throughput protein-protein interaction datasets. Genome Biol 2012; 13:R76. PubMed
  • Bejarano F, Bortolamiol-Becet D, Dai Q, Sun K, Saj A, Chou YT, Raleigh DR, Kim K, Ni JQ, Duan H, Yang JS, Fulga TA, Van Vactor D, Perrimon N, Lai EC. A genome-wide transgenic resource for conditional expression of Drosophila microRNAs. Development 2012; 139:2821-31. PubMed
  • Perrimon N, Pitsouli C, Shilo BZ. Signaling mechanisms controlling cell fate and embryonic patterning. Cold Spring Harb Perspect Biol 2012; 4:a005975. PubMed
  • Katewa SD, Demontis F, Kolipinski M, Hubbard A, Gill MS, Perrimon N, Melov S, Kapahi P. Intramyocellular fatty-acid metabolism plays a critical role in mediating responses to dietary restriction in Drosophila melanogaster. Cell Metab 2012; 16:97-103. PubMed
  • Kusche-Gullberg M, Nybakken K, Perrimon N, Lindahl U. Drosophila heparan sulfate, a novel design. J Biol Chem 2012; 287:21950-6. PubMed
  • Ni JQ, Zhou R, Czech B, Liu LP, Holderbaum L, Yang-Zhou D, Shim HS, Tao R, Handler D, Karpowicz P, Binari R, Booker M, Brennecke J, Perkins LA, Hannon GJ, Perrimon N. A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nat Methods 2011. PubMed
  • Graveley BR, Brooks AN, Carlson JW, Duff MO, Landolin JM, Yang L, Artieri CG, van Baren MJ, Boley N, Booth BW, Brown JB, Cherbas L, Davis CA, Dobin A, Li R, Lin W, Malone JH, Mattiuzzo NR, Miller D, Sturgill D, Tuch BB, Zaleski C, Zhang D, Blanchette M, Dudoit S, Eads B, Green RE, Hammonds A, Jiang L, Kapranov P, Langton L, Perrimon N, Sandler JE, Wan KH, Willingham A, Zhang Y, Zou Y, Andrews J, Bickel PJ, Brenner SE, Brent MR, Cherbas P, Gingeras TR, Hoskins RA, Kaufman TC, Oliver B, Celniker SE. The developmental transcriptome of Drosophila melanogaster. Nature 2011; 471:473-9. PubMed
  • Chan WM, Tsoi H, Wu CC, Wong CH, Cheng TC, Li HY, Lau KF, Shaw PC, Perrimon N, Chan HY. Expanded polyglutamine domain possesses nuclear export activity which modulates subcellular localization and toxicity of polyQ disease protein via exportin-1. Hum Mol Genet 2011. PubMed
  • Gibson WT, Veldhuis JH, Rubinstein B, Cartwright HN, Perrimon N, Brodland GW, Nagpal R, Gibson MC. Control of the mitotic cleavage plane by local epithelial topology. Cell 2011; 144:427-38. PubMed
  • Berezikov E, Robine N, Samsonova A, Westholm JO, Naqvi A, Hung JH, Okamura K, Dai Q, Bortolamiol-Becet D, Martin R, Zhao Y, Zamore PD, Hannon GJ, Marra MA, Weng Z, Perrimon N, Lai EC. Deep annotation of Drosophila melanogaster microRNAs yields insights into their processing, modification, and emergence. Genome Res 2011; 21:203-15. PubMed
  • Cherbas L, Willingham A, Zhang D, Yang L, Zou Y, Eads BD, Carlson JW, Landolin JM, Kapranov P, Dumais J, Samsonova A, Choi JH, Roberts J, Davis CA, Tang H, van Baren MJ, Ghosh S, Dobin A, Bell K, Lin W, Langton L, Duff MO, Tenney AE, Zaleski C, Brent MR, Hoskins RA, Kaufman TC, Andrews J, Graveley BR, Perrimon N, Celniker SE, Gingeras TR, Cherbas P. The transcriptional diversity of 25 Drosophila cell lines. Genome Res 2011; 21:301-14. PubMed
  • Kondo S, Perrimon N. A genome-wide RNAi screen identifies core components of the G₂-M DNA damage checkpoint. Sci Signal 2011; 4:rs1. PubMed
  • Booker M, Samsonova AA, Kwon Y, Flockhart I, Mohr SE, Perrimon N. False negative rates in Drosophila cell-based RNAi screens: a case study. BMC Genomics 2011; 12:50. PubMed
  • Perrimon N, Zirin J, Bai J. Primary Cell Cultures from Drosophila Gastrula Embryos. 2011. PubMed
  • Neumüller RA, Perrimon N. Where gene discovery turns into systems biology: genome-scale RNAi screens in Drosophila. 2011. PubMed
  • , Roy S, Ernst J, Kharchenko PV, Kheradpour P, Negre N, Eaton ML, Landolin JM, Bristow CA, Ma L, Lin MF, Washietl S, Arshinoff BI, Ay F, Meyer PE, Robine N, Washington NL, Di Stefano L, Berezikov E, Brown CD, Candeias R, Carlson JW, Carr A, Jungreis I, Marbach D, Sealfon R, Tolstorukov MY, Will S, Alekseyenko AA, Artieri C, Booth BW, Brooks AN, Dai Q, Davis CA, Duff MO, Feng X, Gorchakov AA, Gu T, Henikoff JG, Kapranov P, Li R, MacAlpine HK, Malone J, Minoda A, Nordman J, Okamura K, Perry M, Powell SK, Riddle NC, Sakai A, Samsonova A, Sandler JE, Schwartz YB, Sher N, Spokony R, Sturgill D, van Baren M, Wan KH, Yang L, Yu C, Feingold E, Good P, Guyer M, Lowdon R, Ahmad K, Andrews J, Berger B, Brenner SE, Brent MR, Cherbas L, Elgin SC, Gingeras TR, Grossman R, Hoskins RA, Kaufman TC, Kent W, Kuroda MI, Orr-Weaver T, Perrimon N, Pirrotta V, Posakony JW, Ren B, Russell S, Cherbas P, Graveley BR, Lewis S, Micklem G, Oliver B, Park PJ, Celniker SE, Henikoff S, Karpen GH, Lai EC, MacAlpine DM, Stein LD, White KP, Kellis M. Identification of functional elements and regulatory circuits by Drosophila modENCODE. Science 2011; 330:1787-97. PubMed
  • Karpowicz P, Perez J, Perrimon N. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development 2010; 137:4135-45. PubMed
  • Zirin J, Perrimon N. Drosophila as a model system to study autophagy. Semin Immunopathol 2010; 32:363-72. PubMed
  • Demontis F, Perrimon N. FOXO/4E-BP signaling in Drosophila muscles regulates organism-wide proteostasis during aging. Cell 2010; 143:813-25. PubMed
  • Rui Y, Bai J, Perrimon N. Sarcomere formation occurs by the assembly of multiple latent protein complexes. PLoS Genet. 2010; 6:e1001208. PubMed
  • Pitsouli C, Perrimon N. Embryonic multipotent progenitors remodel the Drosophila airways during metamorphosis. Development 2010; 137:3615-24. PubMed
  • Micchelli CA, Sudmeier L, Perrimon N, Tang S, Beehler-Evans R. Identification of adult midgut precursors in Drosophila. Gene Expr Patterns 2010. PubMed
  • Schnall-Levin M, Zhao Y, Perrimon N, Berger B. Conserved microRNA targeting in Drosophila is as widespread in coding regions as in 3'UTRs. Proc Natl Acad Sci U S A 2010; 107:15751-6. PubMed
  • Perrimon N, Ni JQ, Perkins L. In vivo RNAi: today and tomorrow. Cold Spring Harb Perspect Biol 2010; 2:a003640. PubMed
  • Rajan A, Perrimon N. Steroids make you bigger? Fat chance says Myc. Cell Metab 2010; 12:7-9. PubMed
  • Bakal C, Perrimon N. Realizing the promise of RNAi high throughput screening. Dev Cell 2010; 18:506-7. PubMed
  • Zhang S, Binari R, Zhou R, Perrimon N. A Genome-wide RNAi Screen for Modifiers of Aggregates Formation by Mutant Huntingtin in Drosophila. Genetics 2010; 184:1165-79. PubMed
  • Nir O, Bakal C, Perrimon N, Berger B. Inference of RhoGAP/GTPase regulation using single-cell morphological data from a combinatorial RNAi screen. Genome Res 2010; 20:372-80. PubMed
  • Karpowicz P, Perrimon N. All for one, and one for all: the clonality of the intestinal stem cell niche. F1000 Biol Rep 2010; 2:73. PubMed
  • Kockel L, Kerr KS, Melnick M, Brückner K, Hebrok M, Perrimon N. Dynamic switch of negative feedback regulation in Drosophila Akt-TOR signaling. PLoS Genet. 2010; 6:e1000990. PubMed
  • Mohr S, Bakal C, Perrimon N. Genomic screening with RNAi: results and challenges. Annu Rev Biochem 2010; 79:37-64. PubMed
  • Yan H, Venkatesan K, Beaver JE, Klitgord N, Yildirim MA, Hao T, Hill DE, Cusick ME, Perrimon N, Roth FP, Vidal M. A genome-wide gene function prediction resource for Drosophila melanogaster. PLoS ONE 2010; 5:e12139. PubMed
  • Apidianakis Y, Pitsouli C, Perrimon N, Rahme L. Synergy between bacterial infection and genetic predisposition in intestinal dysplasia. Proc Natl Acad Sci U S A 2010; 106:20883-8. PubMed
  • Yang X, Friedman A, Nagpal S, Perrimon N, Asara JM. Use of a label-free quantitative platform based on MS/MS average TIC to calculate dynamics of protein complexes in insulin signaling. J Biomol Tech 2009; 20:272-7. PubMed
  • Czech B, Zhou R, Erlich Y, Brennecke J, Binari R, Villalta C, Gordon A, Perrimon N, Hannon GJ. Hierarchical rules for Argonaute loading in Drosophila. Mol Cell 2009; 36:445-56. PubMed
  • Kondo S, Booker M, Perrimon N. Cross-species RNAi rescue platform in Drosophila melanogaster. Genetics 2009; 183:1165-73. PubMed
  • Pitsouli C, Apidianakis Y, Perrimon N. Homeostasis in infected epithelia: stem cells take the lead. Cell Host Microbe 2009; 6:301-7. PubMed
  • Zhou R, Czech B, Brennecke J, Sachidanandam R, Wohlschlegel JA, Perrimon N, Hannon GJ. Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform. RNA 2009; 15:1886-95. PubMed
  • Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML. Droplet microfluidic technology for single-cell high-throughput screening. Proc Natl Acad Sci U S A 2009; 106:14195-200. PubMed
  • Griffin R, Sustar A, Bonvin M, Binari R, del Valle Rodriguez A, Hohl AM, Bateman JR, Villalta C, Heffern E, Grunwald D, Bakal C, Desplan C, Schubiger G, Wu CT, Perrimon N. The twin spot generator for differential Drosophila lineage analysis. Nat Methods 2009; 6:600-2. PubMed
  • Ni JQ,Liu LP,Binari R,Hardy R,Shim HS,Cavallaro A,Booker M,Pfeiffer B,Markstein M,Wang H,Villalta C,Laverty T,Perkins L,Perrimon N. A Drosophila Resource of Transgenic RNAi Lines for Neurogenetics. Genetics 2009; 182:1089-100. PubMed
  • Kaplow IM, Singh R, Friedman A, Bakal C, Perrimon N, Berger B. RNAiCut: automated detection of significant genes from functional genomic screens. Nat Methods 2009; 6:476-7. PubMed
  • Zhang S,Feany MB,Saraswati S,Littleton JT,Perrimon N. Inactivation of Drosophila Huntingtin affects long-term adult functioning and the pathogenesis of a Huntington's disease model. Dis Model Mech 2009; 2:247-66. PubMed
  • Demontis F,Perrimon N. Integration of Insulin receptor/Foxo signaling and dMyc activity during muscle growth regulates body size in Drosophila. Development 2009; 136:983-93. PubMed
  • Wang J,Zhou X,Li F,Bradley PL,Chang SF,Perrimon N,Wong ST. An image score inference system for RNAi genome-wide screening based on fuzzy mixture regression modeling. J Biomed Inform 2008; 42:32-40. PubMed
  • Bai J, Sepp KJ, Perrimon N. Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening. Nat Protoc 2009; 4:1502-12. PubMed
  • Vinegoni C, Razansky D, Pitsouli C, Perrimon N, Ntziachristos V, Weissleder R. Mesoscopic fluorescence tomography for in-vivo imaging of developing Drosophila. 2009. PubMed
  • Zhou R,Hotta I,Denli AM,Hong P,Perrimon N,Hannon GJ. Comparative analysis of argonaute-dependent small RNA pathways in Drosophila. Mol Cell 2008; 32:592-9. PubMed
  • Bakal C,Linding R,Llense F,Heffern E,Martin-Blanco E,Pawson T,Perrimon N. Phosphorylation networks regulating JNK activity in diverse genetic backgrounds. Science 2008; 322:453-6. PubMed
  • Vincent S,Perrimon N,Axelrod JD. Hedgehog and Wingless stabilize but do not induce cell fate during Drosophila dorsal embryonic epidermal patterning. Development 2008; 135:2767-75. PubMed
  • Pitsouli C,Perrimon N. Developmental biology: Our fly cousins' gut. Nature 2008; 454:592-3. PubMed
  • Sepp KJ,Hong P,Lizarraga SB,Liu JS,Mejia LA,Walsh CA,Perrimon N. Identification of neural outgrowth genes using genome-wide RNAi. PLoS Genet 2008; 4:e1000111. PubMed
  • Czech B, Malone CD, Zhou R, Stark A, Schlingeheyde C, Dus M, Perrimon N, Kellis M, Wohlschlegel JA, Sachidanandam R, Hannon GJ, Brennecke J. An endogenous small interfering RNA pathway in Drosophila. Nature 2008; 453:798-802. PubMed
  • Markstein M, Pitsouli C, Villalta C, Celniker SE, Perrimon N. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nat Genet 2008; 40:476-83. PubMed
  • Bai J, Binari R, Ni JQ, Vijayakanthan M, Li HS, Perrimon N. RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance. Development 2008; 135:1439-49. PubMed
  • Philips JA, Porto MC, Wang H, Rubin EJ, Perrimon N. ESCRT factors restrict mycobacterial growth. Proc Natl Acad Sci U S A 2008; 105:3070-5. PubMed
  • Ni JQ, Markstein M, Binari R, Pfeiffer B, Liu LP, Villalta C, Booker M, Perkins L, Perrimon N. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nat Methods 2007; 5:49-51. PubMed
  • Wang J, Zhou X, Bradley PL, Chang SF, Perrimon N, Wong ST. Cellular phenotype recognition for high-content RNA interference genome-wide screening. J Biomol Screen 2008; 13:29-39. PubMed
  • Yin Z,Zhou X,Bakal C,Li F,Sun Y,Perrimon N,Wong ST. Using iterative cluster merging with improved gap statistics to perform online phenotype discovery in the context of high-throughput RNAi screens. BMC Bioinformatics 2008; 9:264. PubMed
  • Bai J, Hartwig JH, Perrimon N. SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth. Dev Cell 2007; 13:828-42. PubMed
  • Silver SJ, Hagen JW, Okamura K, Perrimon N, Lai EC. Functional screening identifies miR-315 as a potent activator of Wingless signaling. Proc Natl Acad Sci U S A 2007; 104:18151-6. PubMed
  • Leopold P, Perrimon N. Drosophila and the genetics of the internal milieu. Nature 2007; 450:186-8. PubMed
  • Bakal C, Aach J, Church G, Perrimon N. Quantitative morphological signatures define local signaling networks regulating cell morphology. Science 2007; 316:1753-6. PubMed
  • Lu J, Ruhf ML, Perrimon N, Leder P. A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression. Proc Natl Acad Sci U S A 2007; 104:9381-6. PubMed
  • Mathey-Prevot B, Perrimon N. Do-it-yourself RNAi made easy? Nat Methods 2007; 4:308-9. PubMed
  • Friedman A, Perrimon N. Genetic screening for signal transduction in the era of network biology. Cell 2007; 128:225-31. PubMed
  • Perrimon N, Friedman A, Mathey-Prevot B, Eggert US. Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens. Drug Discov Today 2007; 12:28-33. PubMed
  • Bach EA, Ekas LA, Ayala-Camargo A, Flaherty MS, Lee H, Perrimon N, Baeg GH. GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo. Gene Expr Patterns 2006; 7:323-31. PubMed
  • Perrimon N, Mathey-Prevot B. Applications of high-throughput RNA interference screens to problems in cell and developmental biology. Genetics 2007; 175:7-16. PubMed
  • Friedman A, Perrimon N. A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Nature 2006; 444:230-4. PubMed
  • Friedman A, Perrimon N. High-throughput approaches to dissecting MAPK signaling pathways. Methods 2006; 40:262-71. PubMed
  • Kulkarni MM, Booker M, Silver SJ, Friedman A, Hong P, Perrimon N, Mathey-Prevot B. Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nat Methods 2006; 3:833-8. PubMed
  • Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG, Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L, Ma Y, Mathey-Pr. Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods 2006; 3:777-9. PubMed
  • Gibson MC, Patel AB, Nagpal R, Perrimon N. The emergence of geometric order in proliferating metazoan epithelia. Nature 2006; 442:1038-41. PubMed
  • Chen LY, Wang JC, Hyvert Y, Lin HP, Perrimon N, Imler JL, Hsu JC. Weckle is a zinc finger adaptor of the toll pathway in dorsoventral patterning of the Drosophila embryo. Curr Biol 2006; 16:1183-93. PubMed
  • Karsten P, Plischke I, Perrimon N, Zeidler MP. Mutational analysis reveals separable DNA binding and trans-activation of Drosophila STAT92E. Cell Signal 2005; 18:819-29. PubMed
  • Echeverri CJ, Perrimon N. High-throughput RNAi screening in cultured cells: a user's guide. Nat Rev Genet 2006; 7:373-84. PubMed
  • Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N, Malhotra V. Functional genomics reveals genes involved in protein secretion and Golgi organization. Nature 2006; 439:604-7. PubMed
  • Micchelli CA, Perrimon N. Evidence that stem cells reside in the adult Drosophila midgut epithelium. Nature 2005; 439:475-9. PubMed
  • Flockhart I, Booker M, Kiger A, Boutros M, Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N, Mathey-Prevot B. FlyRNAi: the Drosophila RNAi screening center database. Nucleic Acids Res 2005; 34:D489-94. PubMed
  • Mathey-Prevot B, Perrimon N. Drosophila genome-wide RNAi screens: are they delivering the promise? Cold Spring Harb Symp Quant Biol 2007; 71:141-8. PubMed
  • Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N. A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway. Nat Genet 2005; 37:1323-32. PubMed
  • Baeg GH, Zhou R, Perrimon N. Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila. Genes Dev 2005; 19:1861-70. PubMed
  • DasGupta R, Kaykas A, Moon RT, Perrimon N. Functional genomic analysis of the Wnt-wingless signaling pathway. Science 2005; 308:826-33. PubMed
  • Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, Field CM. Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets. PLoS Biol 2004; 2:e379. PubMed
  • Boutros M, Kiger AA, Armknecht S, Kerr K, Hild M, Koch B, Haas SA, Paro R, Perrimon N, . Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science 2004; 303:832-5. PubMed
  • Agaisse H, Petersen UM, Boutros M, Mathey-Prevot B, Perrimon N. Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury. Dev Cell 2003; 5:441-50. PubMed
  • Kiger AA, Baum B, Jones S, Jones MR, Coulson A, Echeverri C, Perrimon N. A functional genomic analysis of cell morphology using RNA interference. J Biol 2004; 2:27. PubMed
  • Wills Z, Emerson M, Rusch J, Bikoff J, Baum B, Perrimon N, Van Vactor D. A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding. Neuron 2002; 36:611-22. PubMed
  • Lin X, Buff EM, Perrimon N, Michelson AM. Heparan sulfate proteoglycans are essential for FGF receptor signaling during Drosophila embryonic development. Development 1999; 126:3715-23. PubMed
  • Ghiglione C, Carraway KL, Amundadottir LT, Boswell RE, Perrimon N, Duffy JB. The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis. Cell 1999; 96:847-56. PubMed
  • Ghiglione C, Perrimon N, Perkins LA. Quantitative variations in the level of MAPK activity control patterning of the embryonic termini in Drosophila. Dev Biol 1999; 205:181-93. PubMed
  • Hou XS, Perrimon N. The JAK-STAT pathway in Drosophila. Trends Genet 1997; 13:105-10. PubMed
  • Yu Y, Li W, Su K, Yussa M, Han W, Perrimon N, Pick L. The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz. Nature 1997; 385:552-5. PubMed
  • Kadowaki T, Wilder E, Klingensmith J, Zachary K, Perrimon N. The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing. Genes Dev 1996; 10:3116-28. PubMed
  • Perrimon N, Lanjuin A, Arnold C, Noll E. Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. II. Loci on the second and third chromosomes identified by P-element-induced mutations. Genetics 1996; 144:1681-92. PubMed
  • Perkins LA, Johnson MR, Melnick MB, Perrimon N. The nonreceptor protein tyrosine phosphatase corkscrew functions in multiple receptor tyrosine kinase pathways in Drosophila. Dev Biol 1996; 180:63-81. PubMed
  • Rulifson EJ, Micchelli CA, Axelrod JD, Perrimon N, Blair SS. wingless refines its own expression domain on the Drosophila wing margin. Nature 1996; 384:72-4. PubMed
  • Duffy JB, Perrimon N. Recent advances in understanding signal transduction pathways in worms and flies. Curr Opin Cell Biol 1996; 8:231-8. PubMed
  • Cleghon V, Gayko U, Copeland TD, Perkins LA, Perrimon N, Morrison DK. Drosophila terminal structure development is regulated by the compensatory activities of positive and negative phosphotyrosine signaling sites on the Torso RTK. Genes Dev 1996; 10:566-77. PubMed
  • Perrimon N, Lu X, Hou XS, Hsu JC, Melnick MB, Chou TB, Perkins LA. Dissection of the Torso signal transduction pathway in Drosophila. Mol Reprod Dev 1995; 42:515-22. PubMed
  • Perkins LA, Larsen I, Perrimon N. corkscrew encodes a putative protein tyrosine phosphatase that functions to transduce the terminal signal from the receptor tyrosine kinase torso. Cell 1992; 70:225-36. PubMed
Hide