Photo of Paul D. Allen,  MD, PhD

Paul D. Allen, MD, PhD

Brigham And Women's Hospital

Brigham And Women's Hospital
Phone: (530) 752-5022


paul_allen@hms.harvard.edu

Paul D. Allen, MD, PhD

Brigham And Women's Hospital

EDUCATIONAL TITLES

  • Professor, Emeritus, Harvard Medical School
  • Senior Physician, Anaesthesia, Brigham And Women's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

I am interested to determine if there are specific patterns of RNA expression in the blood that are predictive of different disease states. This is based on the Sentinel Principle in which the blood, which is in contact with all ofthe tissues, will be affected by alterations in the tissue. We have preliminary data to show that it is possible to differentiate between patients with prostate cancer from patients without cancer using this technique, and it is hoped with refinement to become a better predictor than serum PSA measurements. It may also be possible using this technique to develop a diagnostic test for colon and/or breast cancer

Publications

Powered by Harvard Catalyst
  • Yang Z, Liu Q, Mannix RJ, Xu X, Li H, Ma Z, Ingber DE, Allen PD, Wang Y. Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability. Stem Cells 2014. PubMed
  • Szpyt J, Lorenzon N, Perez CF, Norris E, Allen PD, Beam KG, Samsó M. Three-dimensional localization of the α and β subunits and of the II-III loop in the skeletal muscle L-type Ca2+ channel. J Biol Chem 2012. PubMed
  • Liong ML, Lim CR, Yang H, Chao S, Bong CW, Leong WS, Das PK, Loh CS, Lau BE, Yu CG, Ooi EJ, Nam RK, Allen PD, Steele GS, Wassmann K, Richie JP, Liew CC. Blood-based biomarkers of aggressive prostate cancer. PLoS ONE 2012; 7:e45802. PubMed
  • Feng W, Barrientos GC, Cherednichenko G, Yang T, Padilla IT, Truong K, Allen PD, Lopez JR, Pessah IN. Functional and biochemical properties of ryanodine receptor type 1 channels from heterozygous R163C malignant hyperthermia-susceptible mice. Mol Pharmacol 2011; 79:420-31. PubMed
  • Giulivi C, Ross-Inta C, Omanska-Klusek A, Napoli E, Sakaguchi D, Barrientos G, Allen PD, Pessah IN. Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia-susceptible R163C knock-in mice. J Biol Chem 2011; 286:99-113. PubMed
  • Li H, Ding X, Lopez JR, Takeshima H, Ma J, Allen PD, Eltit JM. Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and SR Ca2+ loading in quiescent junctophilin 1 knockout myotubes. J Biol Chem 2010; 285:39171-9. PubMed
  • Feng W, Cherednichenko G, Ward CW, Padilla IT, Cabrales E, Lopez JR, Eltit JM, Allen PD, Pessah IN. Green tea catechins are potent sensitizers of ryanodine receptor type 1 (RyR1). Biochem Pharmacol 2010; 80:512-21. PubMed
  • Westerman KA, Penvose A, Yang Z, Allen PD, Vacanti CA. Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres. Exp Cell Res 2010; 316:1966-76. PubMed
  • Estève E, Eltit JM, Bannister RA, Liu K, Pessah IN, Beam KG, Allen PD, López JR. A malignant hyperthermia-inducing mutation in RYR1 (R163C): alterations in Ca2+ entry, release, and retrograde signaling to the DHPR. J Gen Physiol 2010; 135:619-28. PubMed
  • Bannister RA, Estève E, Eltit JM, Pessah IN, Allen PD, López JR, Beam KG. A malignant hyperthermia-inducing mutation in RYR1 (R163C): consequent alterations in the functional properties of DHPR channels. J Gen Physiol 2010; 135:629-40. PubMed
  • Eltit JM, Yang T, Li H, Molinski T, Pessah IN, Allen PD, Lopez JR. RyR1 mediated Ca2+ leak and Ca2+ entry determine resting intracellular Ca2+ in skeletal myotubes. J Biol Chem 2010; 285:13781-7. PubMed
  • Kashiyama T, Murayama T, Suzuki E, Allen PD, Ogawa Y. Frog alpha- and beta-ryanodine receptors provide distinct intracellular Ca2+ signals in a myogenic cell line. PLoS ONE 2010; 5:e11526. PubMed
  • Allen PD. Triadin, not essential, but useful. J Physiol 2009; 587:3123-4. PubMed
  • McCarthy DA,Macey MG,Allen PD. A simple, novel, procedure for monitoring membrane scrambling and permeability in microparticles, platelets, and leukocytes in whole blood samples. Exp Hematol 2008; 36:909-21. PubMed
  • Voss AA, Allen PD, Pessah IN, Perez CF. Allosterically coupled calcium and magnesium binding sites are unmasked by ryanodine receptor chimeras. Biochem Biophys Res Commun 2007; 366:988-93. PubMed
  • Ison JR, Allen PD. Pre- but not post-menopausal female CBA/CaJ mice show less prepulse inhibition than male mice of the same age. Behav Brain Res 2007; 185:76-81. PubMed
  • Yang T, Allen PD, Pessah IN, Lopez JR. Enhanced excitation-coupled calcium entry in myotubes is associated with expression of RyR1 malignant hyperthermia mutations. J Biol Chem 2007; 282:37471-8. PubMed
  • Yang T, Esteve E, Pessah IN, Molinski TF, Allen PD, Lopez JR. Elevated Resting [Ca2+]i in Myotubes Expressing Malignant Hyperthermia RyR1 cDNAs is Partially Restored by Modulation of Passive Calcium Leak from the Sarcoplasmic Reticulum. Am J Physiol Cell Physiol 2007; 292:C1591-8. PubMed
  • Phimister AJ, Lango J, Lee EH, Ernst-Russell MA, Takeshima H, Ma J, Allen PD, Pessah IN. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem 2007; 282:8667-77. PubMed
  • Lee EH, Song DW, Lee JM, Meissner G, Allen PD, Kim do H. Occurrence of atypical Ca2+ transients in triadin-binding deficient-RYR1 mutants. Biochem Biophys Res Commun 2006; 351:909-14. PubMed
  • Sheridan DC, Takekura H, Franzini-Armstrong C, Beam KG, Allen PD, Perez CF. Bidirectional signaling between calcium channels of skeletal muscle requires multiple direct and indirect interactions. Proc Natl Acad Sci U S A 2006; 103:19760-5. PubMed
  • Yang T, Riehl J, Esteve E, Matthaei KI, Goth S, Allen PD, Pessah IN, Lopez JR. Pharmacologic and functional characterization of malignant hyperthermia in the R163C RyR1 knock-in mouse. Anesthesiology 2006; 105:1164-75. PubMed
  • Moussa CE, Fu Q, Kumar P, Shtifman A, Lopez JR, Allen PD, LaFerla F, Weinberg D, Magrane J, Aprahamian T, Walsh K, Rosen KM, Querfurth HW. Transgenic expression of beta-APP in fast-twitch skeletal muscle leads to calcium dyshomeostasis and IBM-like pathology. FASEB J 2006; 20:2165-7. PubMed
  • Fessenden JD, Feng W, Pessah IN, Allen PD. Amino acid residues Gln4020 and Lys4021 of the ryanodine receptor type 1 are required for activation by 4-chloro-m-cresol. J Biol Chem 2006; 281:21022-31. PubMed
  • Lee EH, Kim do H, Allen PD. Interplay between intra- and extracellular calcium ions. Mol Cells 2006; 21:315-29. PubMed
  • Sams. Structural characterization of the RyR1-FKBP12 interaction. J Mol Biol 2006; 356:917-27. PubMed
Hide