Photo of Paul D. Allen,  MD, PhD

Paul D. Allen, MD, PhD

Brigham And Women's Hospital

Brigham And Women's Hospital
Phone: (530) 752-5022


paul_allen@hms.harvard.edu

Paul D. Allen, MD, PhD

Brigham And Women's Hospital

EDUCATIONAL TITLES

  • Professor, Emeritus, Harvard Medical School
  • Senior Physician, Anaesthesia, Brigham And Women's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

I am interested to determine if there are specific patterns of RNA expression in the blood that are predictive of different disease states. This is based on the Sentinel Principle in which the blood, which is in contact with all ofthe tissues, will be affected by alterations in the tissue. We have preliminary data to show that it is possible to differentiate between patients with prostate cancer from patients without cancer using this technique, and it is hoped with refinement to become a better predictor than serum PSA measurements. It may also be possible using this technique to develop a diagnostic test for colon and/or breast cancer

Publications

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  • Yang Z, Liu Q, Mannix RJ, Xu X, Li H, Ma Z, Ingber DE, Allen PD, Wang Y. Mononuclear cells from dedifferentiation of mouse myotubes display remarkable regenerative capability. Stem Cells 2014. PubMed
  • Szpyt J, Lorenzon N, Perez CF, Norris E, Allen PD, Beam K, Samso M. 3D Localization of the Alpha and Beta Subunits and of the II-III loop in the Skeletal Muscle L-type Ca2+ Channel. J Biol Chem 2012. PubMed
  • Liong ML, Lim CR, Yang H, Chao S, Bong CW, Leong WS, Das PK, Loh CS, Lau BE, Yu CG, Ooi EJ, Nam RK, Allen PD, Steele GS, Wassmann K, Richie JP, Liew CC. Blood-based biomarkers of aggressive prostate cancer. PLoS ONE 2012; 7:e45802. PubMed
  • Feng W, Barrientos GC, Cherednichenko G, Yang T, Padilla IT, Truong K, Allen PD, Lopez JR, Pessah IN. Functional and biochemical properties of ryanodine receptor type 1 channels from heterozygous R163C malignant hyperthermia-susceptible mice. Mol Pharmacol 2011; 79:420-31. PubMed
  • Giulivi C, Ross-Inta C, Omanska-Klusek A, Napoli E, Sakaguchi D, Barrientos G, Allen PD, Pessah IN. Basal bioenergetic abnormalities in skeletal muscle from ryanodine receptor malignant hyperthermia susceptible R163C knock-in mice. J Biol Chem 2011; 286:99-113. PubMed
  • Li H, Ding X, Lopez JR, Takeshima H, Ma J, Allen PD, Eltit JM. Impaired Orai1-mediated resting Ca2+ entry reduces the cytosolic [Ca2+] and SR Ca2+ loading in quiescent junctophilin 1 knockout myotubes. J Biol Chem 2010; 285:39171-9. PubMed
  • Feng W, Cherednichenko G, Ward CW, Padilla IT, Cabrales E, Lopez JR, Eltit JM, Allen PD, Pessah IN. Green tea catechins are potent sensitizers of ryanodine receptor type 1 (RyR1). Biochem Pharmacol 2010; 80:512-21. PubMed
  • Westerman KA, Penvose A, Yang Z, Allen PD, Vacanti CA. Adult muscle 'stem' cells can be sustained in culture as free-floating myospheres. Exp Cell Res 2010; 316:1966-76. PubMed
  • Bannister RA, Estève E, Eltit JM, Pessah IN, Allen PD, López JR, Beam KG. A malignant hyperthermia-inducing mutation in RYR1 (R163C): consequent alterations in the functional properties of DHPR channels. J Gen Physiol 2010; 135:629-40. PubMed
  • Estève E, Eltit JM, Bannister RA, Liu K, Pessah IN, Beam KG, Allen PD, López JR. A malignant hyperthermia-inducing mutation in RYR1 (R163C): alterations in Ca2+ entry, release, and retrograde signaling to the DHPR. J Gen Physiol 2010; 135:619-28. PubMed
  • Eltit JM, Yang T, Li H, Molinski T, Pessah IN, Allen PD, Lopez JR. RyR1 mediated Ca2+ leak and Ca2+ entry determine resting intracellular Ca2+ in skeletal myotubes. J Biol Chem 2010; 285:13781-7. PubMed
  • Kashiyama T, Murayama T, Suzuki E, Allen PD, Ogawa Y. Frog alpha- and beta-ryanodine receptors provide distinct intracellular Ca2+ signals in a myogenic cell line. PLoS ONE 2010; 5:e11526. PubMed
  • Allen PD. Triadin, not essential, but useful. J Physiol 2009; 587:3123-4. PubMed
  • McCarthy DA,Macey MG,Allen PD. A simple, novel, procedure for monitoring membrane scrambling and permeability in microparticles, platelets, and leukocytes in whole blood samples. Exp Hematol 2008; 36:909-21. PubMed
  • Voss AA, Allen PD, Pessah IN, Perez CF. Allosterically coupled calcium and magnesium binding sites are unmasked by ryanodine receptor chimeras. Biochem Biophys Res Commun 2007; 366:988-93. PubMed
  • Yang T, Allen PD, Pessah IN, Lopez JR. Enhanced excitation-coupled calcium entry in myotubes is associated with expression of RyR1 malignant hyperthermia mutations. J Biol Chem 2007; 282:37471-8. PubMed
  • Ison JR, Allen PD. Pre- but not post-menopausal female CBA/CaJ mice show less prepulse inhibition than male mice of the same age. Behav Brain Res 2007; 185:76-81. PubMed
  • Yang T, Esteve E, Pessah IN, Molinski TF, Allen PD, Lopez JR. Elevated Resting [Ca2+]i in Myotubes Expressing Malignant Hyperthermia RyR1 cDNAs is Partially Restored by Modulation of Passive Calcium Leak from the Sarcoplasmic Reticulum. Am J Physiol Cell Physiol 2007; 292:C1591-8. PubMed
  • Phimister AJ, Lango J, Lee EH, Ernst-Russell MA, Takeshima H, Ma J, Allen PD, Pessah IN. Conformation-dependent stability of junctophilin 1 (JP1) and ryanodine receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J Biol Chem 2007; 282:8667-77. PubMed
  • Lee EH, Song DW, Lee JM, Meissner G, Allen PD, Kim do H. Occurrence of atypical Ca2+ transients in triadin-binding deficient-RYR1 mutants. Biochem Biophys Res Commun 2006; 351:909-14. PubMed
  • Sheridan DC, Takekura H, Franzini-Armstrong C, Beam KG, Allen PD, Perez CF. Bidirectional signaling between calcium channels of skeletal muscle requires multiple direct and indirect interactions. Proc Natl Acad Sci U S A 2006; 103:19760-5. PubMed
  • Yang T, Riehl J, Esteve E, Matthaei KI, Goth S, Allen PD, Pessah IN, Lopez JR. Pharmacologic and functional characterization of malignant hyperthermia in the R163C RyR1 knock-in mouse. Anesthesiology 2006; 105:1164-75. PubMed
  • Moussa CE, Fu Q, Kumar P, Shtifman A, Lopez JR, Allen PD, LaFerla F, Weinberg D, Magrane J, Aprahamian T, Walsh K, Rosen KM, Querfurth HW. Transgenic expression of beta-APP in fast-twitch skeletal muscle leads to calcium dyshomeostasis and IBM-like pathology. FASEB J 2006; 20:2165-7. PubMed
  • Fessenden JD, Feng W, Pessah IN, Allen PD. Amino acid residues Gln4020 and Lys4021 of the ryanodine receptor type 1 are required for activation by 4-chloro-m-cresol. J Biol Chem 2006; 281:21022-31. PubMed
  • Lee EH, Kim do H, Allen PD. Interplay between intra- and extracellular calcium ions. Mol Cells 2006; 21:315-29. PubMed
  • Sams. Structural characterization of the RyR1-FKBP12 interaction. J Mol Biol 2006; 356:917-27. PubMed
  • Hurne AM, O'brien JJ, Wingrove D, Cherednichenko G, Allen PD, Beam KG, Pessah IN. Ryanodine receptor type 1 (RyR1) mutations C4958S and C4961S reveal excitation-coupled calcium entry (ECCE) is independent of SR store depletion. J Biol Chem 2005; 280:36994-7004. PubMed
  • Cunha-Neto E, Dzau VJ, Allen PD, Stamatiou D, Benvenutti L, Higuchi ML, Koyama NS, Silva JS, Kalil J, Liew CC. Cardiac gene expression profiling provides evidence for cytokinopathy as a molecular mechanism in Chagas' disease cardiomyopathy. Am J Pathol 2005; 167:305-13. PubMed
  • Sams. Internal structure and visualization of transmembrane domains of the RyR1 calcium release channel by cryo-EM. Nat Struct Mol Biol 2005; 12:539-44. PubMed
  • Perez CF, L. Expression levels of RyR1 and RyR3 control resting free Ca2+ in skeletal muscle. Am J Physiol Cell Physiol 2004; 288:C640-9. PubMed
  • L. Enhanced response to caffeine and 4-chloro-m-cresol in malignant hyperthermia-susceptible muscle is related in part to chronically elevated resting [Ca2+]i. Am J Physiol Cell Physiol 2004; 288:C606-12. PubMed
  • Allen PD. Anesthesia and the human genome project: the quest for accurate prediction of drug responses. Anesthesiology 2005; 102:494-5. PubMed
  • L. Altered Ca2+ homeostasis in human uremic skeletal muscle: possible involvement of cADPR in elevation of intracellular resting [Ca2+]. Nephron Physiol 2005; 100:p51-60. PubMed
  • Fessenden JD, Feng W, Pessah IN, Allen PD. Mutational analysis of putative calcium binding motifs within the skeletal ryanodine receptor isoform, RyR1. J Biol Chem 2004; 279:53028-35. PubMed
  • Christensen RA, Shtifman A, Allen PD, Lopez JR, Querfurth HW. Calcium dyshomeostasis in beta-amyloid and tau-bearing skeletal myotubes. J Biol Chem 2004; 279:53524-32. PubMed
  • Cherednichenko G, Hurne AM, Fessenden JD, Lee EH, Allen PD, Beam KG, Pessah IN. Conformational activation of Ca2+ entry by depolarization of skeletal myotubes. Proc Natl Acad Sci U S A 2004; 101:15793-8. PubMed
  • Lee EH, Rho SH, Kwon SJ, Eom SH, Allen PD, Kim do H. N-terminal region of FKBP12 is essential for binding to the skeletal ryanodine receptor. J Biol Chem 2004; 279:26481-8. PubMed
  • Rivier F, Alkan O, Flint AF, Muskiewicz K, Allen PD, Leboulch P, Gussoni E. Role of bone marrow cell trafficking in replenishing skeletal muscle SP and MP cell populations. J Cell Sci 2004; 117:1979-88. PubMed
  • Protasi F, Shtifman A, Julian FJ, Allen PD. All three ryanodine receptor isoforms generate rapid cooling responses in muscle cells. Am J Physiol Cell Physiol 2003; 286:C662-70. PubMed
  • Zhu X, Ghanta J, Walker JW, Allen PD, Valdivia HH. The calmodulin binding region of the skeletal ryanodine receptor acts as a self-modulatory domain. Cell Calcium 2004; 35:165-77. PubMed
  • Shtifman A, Paolini C, L. Ca2+ influx through alpha1S DHPR may play a role in regulating Ca2+ release from RyR1 in skeletal muscle. Am J Physiol Cell Physiol 2003; 286:C73-8. PubMed
  • Lee EH, Lopez JR, Li J, Protasi F, Pessah IN, Kim do H, Allen PD. Conformational coupling of DHPR and RyR1 in skeletal myotubes is influenced by long-range allosterism: evidence for a negative regulatory module. Am J Physiol Cell Physiol 2003; 286:C179-89. PubMed
  • Perez CF, Mukherjee S, Allen PD. Amino acids 1-1,680 of ryanodine receptor type 1 hold critical determinants of skeletal type for excitation-contraction coupling. Role of divergence domain D2. J Biol Chem 2003; 278:39644-52. PubMed
  • Allen PD. Not all sudden death is the same. Circ Res 2003; 93:484-6. PubMed
  • Bakowska JC, Di Maria MV, Camp SM, Wang Y, Allen PD, Breakefield XO. Targeted transgene integration into transgenic mouse fibroblasts carrying the full-length human AAVS1 locus mediated by HSV/AAV rep(+) hybrid amplicon vector. Gene Ther 2003; 10:1691-702. PubMed
  • Fessenden JD, Perez CF, Goth S, Pessah IN, Allen PD. Identification of a key determinant of ryanodine receptor type 1 required for activation by 4-chloro-m-cresol. J Biol Chem 2003; 278:28727-35. PubMed
  • Yang T, Ta TA, Pessah IN, Allen PD. Functional defects in six ryanodine receptor isoform-1 (RyR1) mutations associated with malignant hyperthermia and their impact on skeletal excitation-contraction coupling. J Biol Chem 2003; 278:25722-30. PubMed
  • Avila G, Lee EH, Perez CF, Allen PD, Dirksen RT. FKBP12 binding to RyR1 modulates excitation-contraction coupling in mouse skeletal myotubes. J Biol Chem 2003; 278:22600-8. PubMed
  • Chen L, Est. Maurocalcine and peptide A stabilize distinct subconductance states of ryanodine receptor type 1, revealing a proportional gating mechanism. J Biol Chem 2003; 278:16095-106. PubMed
  • Perez CF, Voss A, Pessah IN, Allen PD. RyR1/RyR3 chimeras reveal that multiple domains of RyR1 are involved in skeletal-type E-C coupling. Biophys J 2003; 84:2655-63. PubMed
  • Dos Remedios CG, Liew CC, Allen PD, Winslow RL, Van Eyk JE, Dunn MJ. Genomics, proteomics and bioinformatics of human heart failure. J Muscle Res Cell Motil 2003; 24:251-60. PubMed
  • Protasi F, Paolini C, Nakai J, Beam KG, Franzini-Armstrong C, Allen PD. Multiple regions of RyR1 mediate functional and structural interactions with alpha(1S)-dihydropyridine receptors in skeletal muscle. Biophys J 2002; 83:3230-44. PubMed
  • Feng W, Tu J, Yang T, Vernon PS, Allen PD, Worley PF, Pessah IN. Homer regulates gain of ryanodine receptor type 1 channel complex. J Biol Chem 2002; 277:44722-30. PubMed
  • Allen PD, L. "Noninvasive" testing for malignant hyperthermia susceptibility. Anesthesiology 2002; 97:1045-6. PubMed
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