Photo of Priscilla L. Yang,  PhD

Priscilla L. Yang, PhD

Harvard Medical School

Harvard Medical School

Priscilla L. Yang, PhD

Harvard Medical School

EDUCATIONAL TITLES

  • Associate Professor, Microbiology and Immunobiology, Harvard Medical School

DF/HCC PROGRAM AFFILIATION

Research Abstract

 

My group studies mammalian viruses that are significant causes of morbidity and mortality in humans, in particular hepatitis B virus (HBV), hepatitis C virus (HCV), and dengue virus. I have been especially interested in using chemical tools to elucidate molecular mechanisms underlying the replication of viral pathogens and then using this knowledge to pioneer and validate new antiviral targets and strategies. Current themes in my laboratory include the use of small molecules and RNAi as complementary tools to interrogate the role of specific host factors in viral replication; the use of mass spectrometry-based methods to investigate the structure and function of specific lipids in viral replication; the use of activity-based chemoproteomic profiling to characterize virus-induced changes in the host NTPome; the exploitation of the host reactive “cysteineome” to develop irreversible, host-targeted antiviral compounds; and the investigation of polypharmacology as a strategy to develop more effective antivirals with higher barriers to resistance.

Publications

Powered by Harvard Catalyst
  • Vetter ML, Zhang Z, Liu S, Wang J, Cho H, Zhang J, Zhang W, Gray NS, Yang PL. Fluorescent Visualization of Src by Using Dasatinib-BODIPY. Chembiochem 2014; 15:1317-24. PubMed
  • de Wispelaere M, Lacroix AJ, Yang PL. Small molecules AZD0530 and dasatinib inhibit Dengue Virus RNA replication via Fyn kinase. J Virol 2013. PubMed
  • Vetter ML, Rodgers MA, Patricelli MP, Yang PL. Chemoproteomic Profiling Identifies Changes in DNA-PK as Markers of Early Dengue Virus Infection. ACS Chem Biol 2012. PubMed
  • Zhang Z, Kwiatkowski N, Zeng H, Lim SM, Gray NS, Zhang W, Yang PL. Leveraging kinase inhibitors to develop small molecule tools for imaging kinases by fluorescence microscopy. Mol Biosyst 2012; 8:2523-6. PubMed
  • de Wispelaere M, Yang PL. Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol 2012; 86:7072-83. PubMed
  • Rodgers MA, Villareal VA, Schaefer EA, Peng LF, Corey KE, Chung RT, Yang PL. Lipid metabolite profiling identifies desmosterol metabolism as a new antiviral target for hepatitis C virus. J Am Chem Soc 2012; 134:6896-9. PubMed
  • Schmidt AG, Lee K, Yang PL, Harrison SC. Small-molecule inhibitors of dengue-virus entry. PLoS Pathog. 2012; 8:e1002627. PubMed
  • Schmidt AG, Yang PL, Harrison SC. Peptide Inhibitors of Flavivirus Entry Derived from the E-protein Stem. J Virol 2010; 84:12549-54. PubMed
  • Schmidt AG, Yang PL, Harrison SC. Peptide inhibitors of dengue-virus entry target a late-stage fusion intermediate. PLoS Pathog. 2010; 6:e1000851. PubMed
  • Yang PL, Althage A, Chung J, Maier H, Wieland S, Isogawa M, Chisari FV. Immune effectors required for hepatitis B virus clearance. Proc Natl Acad Sci U S A 2010; 107:798-802. PubMed
  • Sessions OM,Barrows NJ,Souza-Neto JA,Robinson TJ,Hershey CL,Rodgers MA,Ramirez JL,Dimopoulos G,Yang PL,Pearson JL,Garcia-Blanco MA. Discovery of insect and human dengue virus host factors. Nature 2009; 458:1047-50. PubMed
  • Rodgers MA,Saghatelian A,Yang PL. Identification of an overabundant cholesterol precursor in hepatitis B virus replicating cells by untargeted lipid metabolite profiling. J Am Chem Soc 2009; 131:5030-1. PubMed
  • Zhang J,Yang PL,Gray NS. Targeting cancer with small molecule kinase inhibitors. Nat Rev Cancer 2008; 9:28-39. PubMed
  • Hwang YC, Chu JJ, Yang PL, Chen W, Yates MV. Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay. Antiviral Res 2008; 77:232-6. PubMed
  • Chu JJ, Yang PL. c-Src protein kinase inhibitors block assembly and maturation of dengue virus. Proc Natl Acad Sci U S A 2007; 104:3520-5. PubMed
  • Yang PL, Althage A, Chung J, Chisari FV. Hydrodynamic injection of viral DNA: a mouse model of acute hepatitis B virus infection. Proc Natl Acad Sci U S A 2002; 99:13825-30. PubMed
  • Yang PL, Schultz PG. Mutational analysis of the affinity maturation of antibody 48G7. J Mol Biol 1999; 294:1191-201. PubMed
  • Patten PA, Gray NS, Yang PL, Marks CB, Wedemayer GJ, Boniface JJ, Stevens RC, Schultz PG. The immunological evolution of catalysis. Science 1996; 271:1086-91. PubMed
Hide