Photo of Richard C. Mulligan,   Ph.D.

Richard C. Mulligan, Ph.D.

Boston Children's Hospital

Boston Children's Hospital
Phone: (617) 432-1465
Fax: (617) 432-6146

Richard C. Mulligan, Ph.D.

Boston Children's Hospital


  • Professor, Pediatrics, Harvard Medical School
  • Ivestigator, Howard Hughes Medical Institute, Boston Children's Hospital



  • Member, Center Scientific Council

Research Abstract

Dr. Mulligan's laboratory is primarily interested in the development of methods for introducing genes into mammalian cells, and the application of those methods in a number of areas of cell biology, developmental biology, virology and medicines. The current research activities of his laboratory are focused in three general areas: (i) the control of hematopoiesis, (ii) manipulation of the immune response via gene transfer, and (iii) the development of new mammalian gene transfer vectors.


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  • Mulligan RC. Development of gene transfer technology. Hum Gene Ther 2014; 25:995-1002. PubMed
  • Burkhardt UE, Hainz U, Stevenson K, Goldstein NR, Pasek M, Naito M, Wu D, Ho VT, Alonso A, Hammond NN, Wong J, Sievers QL, Brusic A, McDonough SM, Zeng W, Perrin A, Brown JR, Canning CM, Koreth J, Cutler C, Armand P, Neuberg D, Lee JS, Antin JH, Mulligan RC, Sasada T, Ritz J, Soiffer RJ, Dranoff G, Alyea EP, Wu CJ. Autologous CLL cell vaccination early after transplant induces leukemia-specific T cells. J Clin Invest 2013; 123:3756-65. PubMed
  • Brusic A, Hainz U, Wadleigh M, Neuberg D, Su M, Canning CM, Deangelo DJ, Stone RM, Lee JS, Mulligan RC, Ritz J, Dranoff G, Sasada T, Wu CJ. Detecting T-cell reactivity to whole cell vaccines: Proof of concept analysis of T-cell response to K562 cell antigens in CML patients. Oncoimmunology 2012; 1:1095-1103. PubMed
  • Sahin E, Colla S, Liesa M, Moslehi J, Müller FL, Guo M, Cooper M, Kotton D, Fabian AJ, Walkey C, Maser RS, Tonon G, Foerster F, Xiong R, Wang YA, Shukla SA, Jaskelioff M, Martin ES, Heffernan TP, Protopopov A, Ivanova E, Mahoney JE, Kost-Alimova M, Perry SR, Bronson R, Liao R, Mulligan R, Shirihai OS, Chin L, DePinho RA. Telomere dysfunction induces metabolic and mitochondrial compromise. Nature 2011; 470:359-65. PubMed
  • Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN. Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. J Clin Invest 2010; 120:379-89. PubMed
  • Bullain SS,Sahin A,Szentirmai O,Sanchez C,Lin N,Baratta E,Waterman P,Weissleder R,Mulligan RC,Carter BS. Genetically engineered T cells to target EGFRvIII expressing glioblastoma. J Neurooncol 2009; 94:373-82. PubMed
  • Yen L,Stockwell BR,Mulligan RC. A mammalian cell-based assay for screening inhibitors of RNA cleavage. Methods Mol Biol 2009; 540:335-47. PubMed
  • Kuhnert F,Tam BY,Sennino B,Gray JT,Yuan J,Jocson A,Nayak NR,Mulligan RC,McDonald DM,Kuo CJ. Soluble receptor-mediated selective inhibition of VEGFR and PDGFRbeta signaling during physiologic and tumor angiogenesis. Proc Natl Acad Sci U S A 2008; 105:10185-90. PubMed
  • Szentirmai O, Baker CH, Bullain SS, Lin N, Takahashi M, Folkman J, Mulligan RC, Carter BS. Successful inhibition of intracranial human glioblastoma multiforme xenograft growth via systemic adenoviral delivery of soluble endostatin and soluble vascular endothelial growth factor receptor-2. J Neurosurg 2008; 108:979-988. PubMed
  • Austin KM, Gupta ML, Coats SA, Tulpule A, Mostoslavsky G, Balazs AB, Mulligan RC, Daley G, Pellman D, Shimamura A. Mitotic spindle destabilization and genomic instability in Shwachman-Diamond syndrome. J Clin Invest 2008; 118:1511-8. PubMed
  • Glodek AM, Le Y, Dykxhoorn DM, Park SY, Mostoslavsky G, Mulligan R, Lieberman J, Beggs HE, Honczarenko M, Silberstein LE. Focal adhesion kinase is required for CXCL12-induced chemotactic and pro-adhesive responses in hematopoietic precursor cells. Leukemia 2007; 21:1723-32. PubMed
  • Chan RK, Garfein E, Gigante PR, Liu P, Agha RA, Mulligan R, Orgill DP. Side population hematopoietic stem cells promote wound healing in diabetic mice. Plast Reconstr Surg 2007; 120:407-11; discussion 412-3. PubMed
  • Butler MO, Lee JS, Ans. Long-Lived Antitumor CD8+ Lymphocytes for Adoptive Therapy Generated Using an Artificial Antigen-Presenting Cell. Clin Cancer Res 2007; 13:1857-1867. PubMed
  • H. Consequences of Shb and c-Abl interactions for cell death in response to various stress stimuli. Exp Cell Res 2006; 313:284-91. PubMed
  • Kaipainen A, Kieran MW, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, Mulligan R, Folkman J, Panigrahy D. PPARalpha Deficiency in Inflammatory Cells Suppresses Tumor Growth. PLoS ONE 2007; 2:e260. PubMed
  • Mostoslavsky G, Fabian AJ, Rooney S, Alt FW, Mulligan RC. Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer. Proc Natl Acad Sci U S A 2006; 103:16406-11. PubMed
  • Murphy GJ, Mostoslavsky G, Kotton DN, Mulligan RC. Exogenous control of mammalian gene expression via modulation of translational termination. Nat Med 2006; 12:1093-9. PubMed
  • Tam BY, Wei K, Rudge JS, Hoffman J, Holash J, Park SK, Yuan J, Hefner C, Chartier C, Lee JS, Jiang S, Niyak NR, Kuypers FA, Ma L, Sundram U, Wu G, Garcia JA, Schrier SL, Maher JJ, Johnson RS, Yancopoulos GD, Mulligan RC, Kuo CJ. VEGF modulates erythropoiesis through regulation of adult hepatic erythropoietin synthesis. Nat Med 2006; 12:793-800. PubMed
  • Yan CT, Kaushal D, Murphy M, Zhang Y, Datta A, Chen C, Monroe B, Mostoslavsky G, Coakley K, Gao Y, Mills KD, Fazeli AP, Tepsuporn S, Hall G, Mulligan R, Fox E, Bronson R, De Girolami U, Lee C, Alt FW. XRCC4 suppresses medulloblastomas with recurrent translocations in p53-deficient mice. Proc Natl Acad Sci U S A 2006; 103:7378-83. PubMed
  • Yen L, Magnier M, Weissleder R, Stockwell BR, Mulligan RC. Identification of inhibitors of ribozyme self-cleavage in mammalian cells via high-throughput screening of chemical libraries. RNA 2006; 12:797-806. PubMed
  • Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS. Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect. Neurosurgery 2006; 58:365-72; discussion 365-72. PubMed
  • Mostoslavsky R, Chua KF, Lombard DB, Pang WW, Fischer MR, Gellon L, Liu P, Mostoslavsky G, Franco S, Murphy MM, Mills KD, Patel P, Hsu JT, Hong AL, Ford E, Cheng HL, Kennedy C, Nunez N, Bronson R, Frendewey D, Auerbach W, Valenzuela D, Karow M, Hottiger MO, Hursting S, Barrett JC, Guarente L, Mulligan R, Demple B, Yancopoulos GD, Alt FW. Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell 2006; 124:315-29. PubMed