Photo of Rohit Kulkarni,  MD, PhD

Rohit Kulkarni, MD, PhD

Harvard Medical School

Harvard Medical School


rohit.kulkarni@joslin.harvard.edu

Rohit Kulkarni, MD, PhD

Harvard Medical School

EDUCATIONAL TITLES

  • Professor, Medicine, Harvard Medical School
  • Senior Investigator, Section of Islet Cell & Regenerative Medicine , Joslin Diabetes Center

DF/HCC PROGRAM AFFILIATION

Research Abstract

The focus of the Kulkarni lab relates to understanding the signaling patwhays that promote growth and proliferation of islet cells. The lab has an interest in molecules that link growth factor signaling with proteins and pathways that also modulate tumor and uncontrolled proliferation. For example, p53 is a transcription factor which is the most frequently altered gene in human cancer. Mice deficient for p53 are developmentally normal but suceptible to spontaneous tumours. p53 is a cellular stress sensor leading to cell-cycle arrest, DNA repair, cellular senescence and apoptosis. We study the coordination and communication between the Insulin/IGF-1 and p53 signaling pathways in pancreatic islet growth. We are also intersted in the relevance of leptin signaling in the regulation of patheays that promote enhanced proliferation of progenitors in the adipocyte and endocrine lineages. We are undertaking studies to examine how mutations in the leptin receptor can modluate the ability of induced pluripotent stem cells to prolferate and differentiate towards different cell types.

Publications

Powered by Harvard Catalyst
  • Valdez IA, Dirice E, Gupta MK, Shirakawa J, Teo AK, Kulkarni RN. Proinflammatory Cytokines Induce Endocrine Differentiation in Pancreatic Ductal Cells via STAT3-Dependent NGN3 Activation. Cell Rep 2016; 15:460-70. PubMed
  • Dirice E, Walpita D, Vetere A, Meier BC, Kahraman S, Hu J, Dančík V, Burns SM, Gilbert TJ, Olson DE, Clemons PA, Kulkarni RN, Wagner BK. Inhibition of DYRK1A stimulates human beta-cell proliferation. Diabetes 2016. PubMed
  • Dhawan S, Dirice E, Kulkarni RN, Bhushan A. Inhibition of TGF-beta signaling promotes human pancreatic beta cell replication. Diabetes 2016. PubMed
  • El Ouaamari A, Dirice E, Gedeon N, Hu J, Zhou JY, Shirakawa J, Hou L, Goodman J, Karampelias C, Qiang G, Boucher J, Martinez R, Gritsenko MA, De Jesus DF, Kahraman S, Bhatt S, Smith RD, Beer HD, Jungtrakoon P, Gong Y, Goldfine AB, Liew CW, Doria A, Andersson O, Qian WJ, Remold-O'Donnell E, Kulkarni RN. SerpinB1 Promotes Pancreatic β Cell Proliferation. Cell Metab 2016; 23:194-205. PubMed
  • Ghanem SS, Heinrich G, Lester SG, Pfeiffer V, Bhattacharya S, Patel PR, DeAngelis AM, Dai T, Ramakrishnan SK, Smiley ZN, Jung DY, Lee Y, Kitamura T, Ergun S, Kulkarni RN, Kim JK, Giovannucci DR, Najjar SM. Increased Glucose-induced Secretion of Glucagon-like Peptide-1 in Mice Lacking the Carcino-Embryonic Antigen-related Cell Adhesion Molecule 2. J Biol Chem 2015. PubMed
  • Gupta MK, Teo AK, Rao TN, Bhatt S, Kleinridders A, Shirakawa J, Takatani T, Hu J, De Jesus DF, Windmueller R, Wagers AJ, Kulkarni RN. Excessive Cellular Proliferation Negatively Impacts Reprogramming Efficiency of Human Fibroblasts. Stem Cells Transl Med 2015; 4:1101-8. PubMed
  • Lee YJ, Liu C, Liao M, Sukhova GK, Shirakawa J, Abdennour M, Iamarene K, Andre S, Inouye K, Clement K, Kulkarni RN, Banks AS, Libby P, Shi GP. Deficiency of FcϵR1 increases body weight gain but improves glucose tolerance in diet-induced obese mice. Endocrinology 2015. PubMed
  • Bhatt S, Gupta MK, Khamaisi M, Martinez R, Gritsenko MA, Wagner BK, Guye P, Busskamp V, Shirakawa J, Wu G, Liew CW, Clauss TR, Valdez I, El Ouaamari A, Dirice E, Takatani T, Keenan HA, Smith RD, Church G, Weiss R, Wagers AJ, Qian WJ, King GL, Kulkarni RN. Preserved DNA Damage Checkpoint Pathway Protects against Complications in Long-Standing Type 1 Diabetes. Cell Metab 2015; 22:239-52. PubMed
  • El Ouaamari A, Zhou J, Liew CW, Shirakawa J, Dirice E, Gedeon N, Kahraman S, De Jesus DF, Bhatt S, Kim JS, Clauss TR, Camp DG, Smith RD, Qian WJ, Kulkarni RN. Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics. J Proteome Res 2015. PubMed
  • Al-Share QY, DeAngelis AM, Lester SG, Bowman TA, Ramakrishnan SK, Abdallah SL, Russo L, Patel PR, Kaw MK, Raphael CK, Kim AJ, Heinrich G, Lee AD, Kim JK, Kulkarni RN, Philbrick WM, Najjar SM. Forced Hepatic Over-expression of CEACAM1 Curtails Diet-induced Insulin Resistance. Diabetes 2015. PubMed
  • Rao TN, Marks-Bluth J, Sullivan J, Gupta MK, Chandrakanthan V, Fitch SR, Ottersbach K, Jang YC, Piao X, Kulkarni RN, Serwold T, Pimanda JE, Wagers AJ. High-level Gpr56 expression is dispensable for the maintenance and function of hematopoietic stem and progenitor cells in mice. 2015; 14:307-322. PubMed
  • Kahraman S, Dirice E, De Jesus DF, Hu J, Kulkarni RN. Maternal insulin resistance and transient hyperglycemia impacts the metabolic and endocrine phenotypes of offspring. Am J Physiol Endocrinol Metab 2014. PubMed
  • Dirice E, Kulkarni RN*. Harnessing immune cells to enhance -cell mass in type 1 diabetes. J Invest Med 2016; 64:14-20.
Hide