Photo of Ulrich H. von Andrian,  MD

Ulrich H. von Andrian, MD

Harvard Medical School

Harvard Medical School
Phone: (617) 432-6827
Fax: (617) 432-6829


uva@hms.harvard.edu

Ulrich H. von Andrian, MD

Harvard Medical School

EDUCATIONAL TITLES

  • Edward Mallinckrodt Jr. Professor of Immunopathology, Microbiology and Immunobiology, Harvard Medical School
  • Senior Investigator, von Andrian Lab, Boston Children's Hospital

DF/HCC PROGRAM AFFILIATION

Research Abstract

My research seeks answers to the question how circulating blood cells find their way in the body. Directed migration of blood-borne cells to distinct target tissues can be observed in embryos as soon as the circulatory system is established and plays a critical role throughout life in numerous physiologic and pathologic conditions. Despite considerable progress in this field, it is still beyond the reach of even the most sophisticated in vitro methodology to simulate the complex interplay of physical, cellular, biochemical, and other factors that determine blood cell behavior in microvessels. Therefore, we employ intravital microscopy to study the molecular mechanisms of interactions between blood cells and the vascular wall by direct observation in anesthetized mice. Using this approach, we have demonstrated that blood cell homing to most target tissues requires an initial tethering step that leads to rolling in postcapillary venules and is followed by an activation step which, in turn, triggers stationary adhesion and emigration. Each of these three steps (i.e. 1. rolling; 2. chemotactic stimulation; and 3. firm arrest) involves distinct molecular pathways whose unique combination is the reason why certain blood cells migrate to a particular organ, whereas others don't.

We are now dissecting the site-specific adhesion cascades that direct myeloid and lymphoid cells, hematopoietic stem cells and platelets to normal and diseased tissues. We have established models in mice that allow quantitative observations in Peyer's patches; gut; bladder; striated muscle; skin; pancreas; liver; knee joint; bone marrow; bone; and peripheral lymph nodes. The techniques for observing the latter three tissues were developed in my laboratory.

Understanding how lymphocytes, in particular T cells, home to and migrate within peripheral lymph nodes is a major focus of my group. To this end, we are using a panel of mice that are genetically deficient in specific adhesion pathways. We have also generated transgenic mouse strains that express fluorescent proteins in distinct T cell subsets. We are using these mice to study how T cells differentiate into effector and memory subsets; how this differentiation affects their migratory properties; and how antigen-presenting dendritic cells influence these processes. T cells and dendritic cells can be visualized in the intra- and extravascular space by intravital microscopy using both single- and multi-photon fluorescence techniques. This allows us to dissect the trafficking behavior of these immune cells at a resolution and specificity that could not be achieved with other methods.

Besides supervising and instructing the students and fellows in my laboratory (15-20 members), my current teaching activities include the co-direction and organization of the Immunology 201 and Immunology 202 semester courses for HMS graduate students. In addition, we have numerous collaborators in the Harvard community and elsewhere who perform intravital microscopy studies in our facility under my supervision. I also lecture regularly to HMS graduate students, postdoctoral fellows and staff about leukocyte adhesion, migration and homing.

Publications

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  • Gerlach C, Loughhead SM, von Andrian UH. Figuring fact from fiction: unbiased polling of memory T cells. Cell 2015; 161:702-4. PubMed
  • Maldonado RA, LaMothe RA, Ferrari JD, Zhang AH, Rossi RJ, Kolte PN, Griset AP, O'Neil C, Altreuter DH, Browning E, Johnston L, Farokhzad OC, Langer R, Scott DW, von Andrian UH, Kishimoto TK. Polymeric synthetic nanoparticles for the induction of antigen-specific immunological tolerance. Proc Natl Acad Sci U S A 2015; 112:E156-65. PubMed
  • Sharei A, Trifonova R, Jhunjhunwala S, Hartoularos GC, Eyerman AT, Lytton-Jean A, Angin M, Sharma S, Poceviciute R, Mao S, Heimann M, Liu S, Talkar T, Khan OF, Addo M, von Andrian UH, Anderson DG, Langer R, Lieberman J, Jensen KF. Ex vivo cytosolic delivery of functional macromolecules to immune cells. PLoS ONE 2015; 10:e0118803. PubMed
  • Lynch L, Michelet X, Zhang S, Brennan PJ, Moseman A, Lester C, Besra G, Vomhof-Dekrey EE, Tighe M, Koay HF, Godfrey DI, Leadbetter EA, Sant'Angelo DB, von Andrian U, Brenner MB. Regulatory iNKT cells lack expression of the transcription factor PLZF and control the homeostasis of T(reg) cells and macrophages in adipose tissue. Nat Immunol 2014; 16:85-95. PubMed
  • Sage PT, Alvarez D, Godec J, von Andrian UH, Sharpe AH. Circulating T follicular regulatory and helper cells have memory-like properties. J Clin Invest 2014; 124:5191-204. PubMed
  • Choi WI, Kamaly N, Riol-Blanco L, Lee IH, Wu J, Swami A, Vilos C, Yameen B, Yu M, Shi J, Tabas I, von Andrian UH, Jon S, Farokhzad OC. A Solvent-Free Thermosponge Nanoparticle Platform for Efficient Delivery of Labile Proteins. Nano Lett 2014. PubMed
  • Textor J, Henrickson SE, Mandl JN, von Andrian UH, Westermann J, de Boer RJ, Beltman JB. Random migration and signal integration promote rapid and robust T cell recruitment. PLoS Comput. Biol. 2014; 10:e1003752. PubMed
  • Dahlman JE, Barnes C, Khan O, Thiriot A, Jhunjunwala S, Shaw TE, Xing Y, Sager HB, Sahay G, Speciner L, Bader A, Bogorad RL, Yin H, Racie T, Dong Y, Jiang S, Seedorf D, Dave A, Sandu KS, Webber MJ, Novobrantseva T, Ruda VM, Lytton-Jean AK, Levins CG, Kalish B, Mudge DK, Perez M, Abezgauz L, Dutta P, Smith L, Charisse K, Kieran MW, Fitzgerald K, Nahrendorf M, Danino D, Tuder RM, von Andrian UH, Akinc A, Panigrahy D, Schroeder A, Kotelianski V, Langer R, Anderson DG. In vivo endothelial siRNA delivery using polymeric nanoparticles with low molecular weight. Nat Nanotechnol 2014. PubMed
  • Riol-Blanco L, Ordovas-Montanes J, Perro M, Naval E, Thiriot A, Alvarez D, Wood JN, von Andrian UH. Nociceptive sensory neurons drive interleukin-23-mediated psoriasiform skin inflammation. Nature 2014. PubMed
  • Kumar L, Chou J, Yee CS, Borzutzky A, Vollmann EH, von Andrian UH, Park SY, Hollander G, Manis JP, Poliani PL, Geha RS. Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function. J Exp Med 2014. PubMed
  • Ilyinskii PO, Roy CJ, O'Neil CP, Browning EA, Pittet LA, Altreuter DH, Alexis F, Tonti E, Shi J, Basto PA, Iannacone M, Radovic-Moreno AF, Langer RS, Farokhzad OC, von Andrian UH, Johnston LP, Kishimoto TK. Adjuvant-carrying synthetic vaccine particles augment the immune response to encapsulated antigen and exhibit strong local immune activation without inducing systemic cytokine release. Vaccine 2014. PubMed
  • Krause DS, Lazarides K, Lewis JB, von Andrian UH, Van Etten RA. Selectins and their ligands are required for homing and engraftment of BCR-ABL1+ leukemic stem cells in the bone marrow niche. Blood 2014; 123:1361-71. PubMed
  • Henrickson SE, Perro M, Loughhead SM, Senman B, Stutte S, Quigley M, Alexe G, Iannacone M, Flynn MP, Omid S, Jesneck JL, Imam S, Mempel TR, Mazo IB, Haining WN, von Andrian UH. Antigen availability determines CD8竅コ T cell-dendritic cell interaction kinetics and memory fate decisions. Immunity 2013; 39:496-507. PubMed
  • Gerlach C, Thiriot A, von Andrian UH. Of origins and pedigrees: lineage tracing of dendritic cells. Cell 2013; 154:720-2. PubMed
  • Majewska-Szczepanik M, Paust S, von Andrian UH, Askenase PW, Szczepanik M. Nk Cell Mediated Contact Sensitivity Develops Rapidly And Depends On Ifn-Α, Ifn-Γ And Il-12. Immunology 2013. PubMed
  • Wang C, Yi T, Qin L, Maldonado RA, von Andrian UH, Kulkarni S, Tellides G, Pober JS. Rapamycin-treated human endothelial cells preferentially activate allogeneic regulatory T cells. J Clin Invest 2013; 123:1677-93. PubMed
  • Sperandio M, Quackenbush EJ, Sushkova N, Altstätter J, Nussbaum C, Schmid S, Pruenster M, Kurz A, Margraf A, Steppner A, Schweiger N, Borsig L, Boros I, Krajewski N, Genzel-Boroviczeny O, Jeschke U, Frommhold D, von Andrian UH. Ontogenetic regulation of leukocyte recruitment in mouse yolk sac vessels. Blood 2013. PubMed
  • Nussbaum C, Gloning A, Pruenster M, Frommhold D, Bierschenk S, Genzel-Boroviczény O, von Andrian UH, Quackenbush E, Sperandio M. Neutrophil and endothelial adhesive function during human fetal ontogeny. J Leukoc Biol 2013; 93:175-84. PubMed
  • Groom JR, Richmond J, Murooka TT, Sorensen EW, Sung JH, Bankert K, von Andrian UH, Moon JJ, Mempel TR, Luster AD. CXCR3 chemokine receptor-ligand interactions in the lymph node optimize CD4+ T helper 1 cell differentiation. Immunity 2012; 37:1091-103. PubMed
  • Zhang L, Orban M, Lorenz M, Barocke V, Braun D, Urtz N, Schulz C, von Brühl ML, Tirniceriu A, Gaertner F, Proia RL, Graf T, Bolz SS, Montanez E, Prinz M, Müller A, von Baumgarten L, Billich A, Sixt M, Fässler R, von Andrian UH, Junt T, Massberg S. A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis. J Exp Med 2012; 209:2165-81. PubMed
  • Sung JH, Zhang H, Moseman EA, Alvarez D, Iannacone M, Henrickson SE, de la Torre JC, Groom JR, Luster AD, von Andrian UH. Chemokine guidance of central memory T cells is critical for antiviral recall responses in lymph nodes. Cell 2012; 150:1249-63. PubMed
  • Thomas GM, Carbo C, Curtis BR, Martinod K, Mazo IB, Schatzberg D, Cifuni SM, Fuchs TA, von Andrian UH, Hartwig JH, Aster RH, Wagner DD. Extracellular DNA traps are associated with the pathogenesis of TRALI in humans and mice. Blood 2012; 119:6335-43. PubMed
  • Allen SJ, Hamrah P, Gate DM, Mott KR, Mantopoulos D, Zheng L, Town T, Jones C, von Andrian UH, Freeman GJ, Sharpe AH, Benmohamed L, Ahmed R, Wechsler SL, Ghiasi H. The role of LAT in increased CD8+ T cell exhaustion in trigeminal ganglia of mice latently infected with herpes simplex virus type 1. J Virol 2011. PubMed
  • Paust S, Gill HS, Wang BZ, Flynn MP, Moseman EA, Senman B, Szczepanik M, Telenti A, Askenase PW, Compans RW, von Andrian UH. Critical role for the chemokine receptor CXCR6 in NK cell-mediated antigen-specific memory of haptens and viruses. Nat Immunol 2010; 11:1127-35. PubMed
  • Bao X, Moseman EA, Saito H, Petryanik B, Thiriot A, Hatakeyama S, Ito Y, Kawashima H, Yamaguchi Y, Lowe JB, von Andrian UH, Fukuda M. Endothelial heparan sulfate controls chemokine presentation in recruitment of lymphocytes and dendritic cells to lymph nodes. Immunity 2010; 33:817-29. PubMed
  • Boscacci RT, Pfeiffer F, Gollmer K, Checa Sevilla AI, Martin AM, Soriano SF, Natale D, Henrickson S, von Andrian UH, Fukui Y, Mellado M, Deutsch U, Engelhardt B, Stein JV. Comprehensive analysis of lymph node stroma-expressed Ig superfamily members reveals redundant and non-redundant roles for ICAM-1, ICAM-2, and VCAM-1 in lymphocyte homing. Blood 2010; 116:915-25. PubMed
  • Iannacone M, Moseman EA, Tonti E, Bosurgi L, Junt T, Henrickson SE, Whelan SP, Guidotti LG, von Andrian UH. Subcapsular sinus macrophages prevent CNS invasion on peripheral infection with a neurotropic virus. Nature 2010; 465:1079-83. PubMed
  • Paust S, Senman B, von Andrian UH. Adaptive immune responses mediated by natural killer cells. Immunol Rev 2010; 235:286-96. PubMed
  • Park EJ, Peixoto A, Imai Y, Goodarzi A, Cheng G, Carman CV, von Andrian UH, Shimaoka M. Distinct roles for LFA-1 affinity regulation during T-cell adhesion, diapedesis, and interstitial migration in lymph nodes. Blood 2010; 115:1572-81. PubMed
  • Mamo T, Moseman EA, Kolishetti N, Salvador-Morales C, Shi J, Kuritzkes DR, Langer R, von Andrian U, Farokhzad OC. Emerging nanotechnology approaches for HIV/AIDS treatment and prevention. Nanomed 2010; 5:269-85. PubMed
  • Wolf K, Alexander S, Schacht V, Coussens LM, von Andrian UH, van Rheenen J, Deryugina E, Friedl P. Collagen-based cell migration models in vitro and in vivo. Semin Cell Dev Biol 2009; 20:931-41. PubMed
  • Massberg S, von Andrian UH. Novel trafficking routes for hematopoietic stem and progenitor cells. Ann N Y Acad Sci 2009; 1176:87-93. PubMed
  • Beltman JB, Henrickson SE, von Andrian UH, de Boer RJ, Marée AF. Towards estimating the true duration of dendritic cell interactions with T cells. J Immunol Methods 2009; 347:54-69. PubMed
  • Sarraj B,Massberg S,Li Y,Kasorn A,Subramanian K,Loison F,Silberstein LE,von Andrian U,Luo HR. Myeloid-specific deletion of tumor suppressor PTEN augments neutrophil transendothelial migration during inflammation. J Immunol 2009; 182:7190-200. PubMed
  • Schulz C,Von Andrian UH,Massberg S. Trafficking of Murine Hematopoietic Stem and Progenitor Cells in Health and Vascular Disease. Microcirculation 2009. PubMed
  • Roozendaal R,Mempel TR,Pitcher LA,Gonzalez SF,Verschoor A,Mebius RE,von Andrian UH,Carroll MC. Conduits mediate transport of low-molecular-weight antigen to lymph node follicles. Immunity 2009; 30:264-76. PubMed
  • Mora JR,von Andrian UH. Role of retinoic acid in the imprinting of gut-homing IgA-secreting cells. Semin Immunol 2008; 21:28-35. PubMed
  • Schulz C,von Andrian UH,Massberg S. Hematopoietic stem and progenitor cells: their mobilization and homing to bone marrow and peripheral tissue. Immunol Res 2009; 44:160-8. PubMed
  • Imai Y,Park EJ,Peer D,Peixoto A,Cheng G,von Andrian UH,Carman CV,Shimaoka M. Genetic perturbation of the putative cytoplasmic membrane-proximal salt bridge aberrantly activates alpha(4) integrins. Blood 2008; 112:5007-15. PubMed
  • Alvarez D,Vollmann EH,von Andrian UH. Mechanisms and consequences of dendritic cell migration. Immunity 2008; 29:325-42. PubMed
  • Mora JR,Iwata M,von Andrian UH. Vitamin effects on the immune system: vitamins A and D take centre stage. Nat Rev Immunol 2008. PubMed
  • Zheng H, Jin B, Henrickson S, Perelson AS, von Andrian UH, Chakraborty AK. How antigen quantity and quality determine T cell decisions in lymphoid tissue. Mol Cell Biol 2008; 28:4040-51. PubMed
  • Henrickson SE, Mempel TR, Mazo IB, Liu B, Artyomov MN, Zheng H, Peixoto A, Flynn MP, Senman B, Junt T, Wong HC, Chakraborty AK, von Andrian UH. T cell sensing of antigen dose governs interactive behavior with dendritic cells and sets a threshold for T cell activation. Nat Immunol 2008; 9:282-91. PubMed
  • Iparraguirre A, Tobias JW, Hensley SE, Masek KS, Cavanagh LL, Rendl M, Hunter CA, Ertl HC, von Andrian UH, Weninger W. Two distinct activation states of plasmacytoid dendritic cells induced by influenza virus and CpG 1826 oligonucleotide. J Leukoc Biol 2007; 83:610-20. PubMed
  • Laird DJ, von Andrian UH, Wagers AJ. Stem cell trafficking in tissue development, growth, and disease. Cell 2008; 132:612-30. PubMed
  • Henrickson SE, Mempel TR, Mazo IB, Liu B, Artyomov MN, Zheng H, Peixoto A, Flynn M, Senman B, Junt T, Wong HC, Chakraborty AK, von Andrian UH. In vivo imaging of T cell priming. Sci Signal 2008; 1:pt2. PubMed
  • Tenno M, Ohtsubo K, Hagen FK, Ditto D, Zarbock A, Schaerli P, von Andrian UH, Ley K, Le D, Tabak LA, Marth JD. Initiation of protein O glycosylation by the polypeptide GalNAcT-1 in vascular biology and humoral immunity. Mol Cell Biol 2007; 27:8783-96. PubMed
  • Massberg S, Schaerli P, Knezevic-Maramica I, Kollnberger M, Tubo N, Moseman EA, Huff IV, Junt T, Wagers AJ, Mazo IB, von Andrian UH. Immunosurveillance by hematopoietic progenitor cells trafficking through blood, lymph, and peripheral tissues. Cell 2007; 131:994-1008. PubMed
  • Junt T, Moseman EA, Iannacone M, Massberg S, Lang PA, Boes M, Fink K, Henrickson SE, Shayakhmetov DM, Di Paolo NC, van Rooijen N, Mempel TR, Whelan SP, von Andrian UH. Subcapsular sinus macrophages in lymph nodes clear lymph-borne viruses and present them to antiviral B cells. Nature 2007; 450:110-4. PubMed
  • Bonasio R, Carman CV, Kim E, Sage PT, Love KR, Mempel TR, Springer TA, von Andrian UH. Specific and covalent labeling of a membrane protein with organic fluorochromes and quantum dots. Proc Natl Acad Sci U S A 2007; 104:14753-8. PubMed
  • Park EJ, Mora JR, Carman CV, Chen J, Sasaki Y, Cheng G, von Andrian UH, Shimaoka M. Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut. J Clin Invest 2007; 117:2526-38. PubMed
  • Henrickson SE, von Andrian UH. Single-cell dynamics of T-cell priming. Curr Opin Immunol 2007; 19:249-58. PubMed
  • Swirski FK, Berger CR, Figueiredo JL, Mempel TR, von Andrian UH, Pittet MJ, Weissleder R. A near-infrared cell tracker reagent for multiscopic in vivo imaging and quantification of leukocyte immune responses. PLoS ONE 2007; 2:e1075. PubMed
  • Mora JR, Iwata M, Eksteen B, Song SY, Junt T, Senman B, Otipoby KL, Yokota A, Takeuchi H, Ricciardi-Castagnoli P, Rajewsky K, Adams DH, von Andrian UH. Generation of gut-homing IgA-secreting B cells by intestinal dendritic cells. Science 2006; 314:1157-60. PubMed
  • Pearce G, Angeli V, Randolph GJ, Junt T, von Andrian U, Schnittler HJ, Jessberger R. Signaling protein SWAP-70 is required for efficient B cell homing to lymphoid organs. Nat Immunol 2006; 7:827-34. PubMed
  • Mempel TR, Pittet MJ, Khazaie K, Weninger W, Weissleder R, von Boehmer H, von Andrian UH. Regulatory T cells reversibly suppress cytotoxic T cell function independent of effector differentiation. Immunity 2006; 25:129-41. PubMed
  • Scimone ML, Aifantis I, Apostolou I, von Boehmer H, von Andrian UH. A multistep adhesion cascade for lymphoid progenitor cell homing to the thymus. Proc Natl Acad Sci U S A 2006; 103:7006-11. PubMed
  • O'Leary JG, Goodarzi M, Drayton DL, von Andrian UH. T cell- and B cell-independent adaptive immunity mediated by natural killer cells. Nat Immunol 2006; 7:507-16. PubMed
  • Wang J, Fathman JW, Lugo-Villarino G, Scimone L, von Andrian U, Dorfman DM, Glimcher LH. Transcription factor T-bet regulates inflammatory arthritis through its function in dendritic cells. J Clin Invest 2006; 116:414-21. PubMed
  • Luster AD, Alon R, von Andrian UH. Immune cell migration in inflammation: present and future therapeutic targets. Nat Immunol 2005; 6:1182-90. PubMed
  • Uchimura K, Gauguet JM, Singer MS, Tsay D, Kannagi R, Muramatsu T, von Andrian UH, Rosen SD. A major class of L-selectin ligands is eliminated in mice deficient in two sulfotransferases expressed in high endothelial venules. Nat Immunol 2005; 6:1105-13. PubMed
  • Cavanagh LL, Bonasio R, Mazo IB, Halin C, Cheng G, van der Velden AW, Cariappa A, Chase C, Russell P, Starnbach MN, Koni PA, Pillai S, Weninger W, von Andrian UH. Activation of bone marrow-resident memory T cells by circulating, antigen-bearing dendritic cells. Nat Immunol 2005; 6:1029-37. PubMed
  • Cariappa A, Mazo IB, Chase C, Shi HN, Liu H, Li Q, Rose H, Leung H, Cherayil BJ, Russell P, von Andrian U, Pillai S. Perisinusoidal B cells in the bone marrow participate in T-independent responses to blood-borne microbes. Immunity 2005; 23:397-407. PubMed
  • Halin C, Scimone ML, Bonasio R, Gauguet JM, Mempel TR, Quackenbush E, Proia RL, Mandala S, von Andrian UH. The S1P-analog FTY720 differentially modulates T-cell homing via HEV: T-cell-expressed S1P1 amplifies integrin activation in peripheral lymph nodes but not in Peyer patches. Blood 2005; 106:1314-22. PubMed
  • Halin C, Mora JR, Sumen C, von Andrian UH. In Vivo Imaging of Lymphocyte Trafficking. Annu Rev Cell Dev Biol 2005. PubMed
  • Snapper SB, Meelu P, Nguyen D, Stockton BM, Bozza P, Alt FW, Rosen FS, von Andrian UH, Klein C. WASP deficiency leads to global defects of directed leukocyte migration in vitro and in vivo. J Leukoc Biol 2005; 77:993-8. PubMed
  • Shamri R, Grabovsky V, Gauguet JM, Feigelson S, Manevich E, Kolanus W, Robinson MK, Staunton DE, von Andrian UH, Alon R. Lymphocyte arrest requires instantaneous induction of an extended LFA-1 conformation mediated by endothelium-bound chemokines. Nat Immunol 2005; 6:497-506. PubMed
  • Mazo IB, Honczarenko M, Leung H, Cavanagh LL, Bonasio R, Weninger W, Engelke K, Xia L, McEver RP, Koni PA, Silberstein LE, von Andrian UH. Bone marrow is a major reservoir and site of recruitment for central memory CD8+ T cells. Immunity 2005; 22:259-70. PubMed
  • Mora JR, Cheng G, Picarella D, Briskin M, Buchanan N, von Andrian UH. Reciprocal and dynamic control of CD8 T cell homing by dendritic cells from skin- and gut-associated lymphoid tissues. J Exp Med 2005; 201:303-16. PubMed
  • Halin C, Rodrigo Mora J, Sumen C, von Andrian UH. In vivo imaging of lymphocyte trafficking. Annu Rev Cell Dev Biol 2005; 21:581-603. PubMed
  • Gauguet JM, Rosen SD, Marth JD, von Andrian UH. Core 2 branching beta1,6-N-acetylglucosaminyltransferase and high endothelial cell N-acetylglucosamine-6-sulfotransferase exert differential control over B- and T-lymphocyte homing to peripheral lymph nodes. Blood 2004; 104:4104-12. PubMed
  • Stokol T, O'Donnell P, Xiao L, Knight S, Stavrakis G, Botto M, von Andrian UH, Mayadas TN. C1q governs deposition of circulating immune complexes and leukocyte Fcgamma receptors mediate subsequent neutrophil recruitment. J Exp Med 2004; 200:835-46. PubMed
  • Mora JR, von Andrian UH. Retinoic acid: an educational "vitamin elixir" for gut-seeking T cells. Immunity 2004; 21:458-60. PubMed
  • Bergmeier W, Piffath CL, Cheng G, Dole VS, Zhang Y, von Andrian UH, Wagner DD. Tumor necrosis factor-alpha-converting enzyme (ADAM17) mediates GPIbalpha shedding from platelets in vitro and in vivo. Circ Res 2004; 95:677-83. PubMed
  • Sumen C, Mempel TR, Mazo IB, von Andrian UH. Intravital microscopy: visualizing immunity in context. Immunity 2004; 21:315-29. PubMed
  • Mempel TR, Scimone ML, Mora JR, von Andrian UH. In vivo imaging of leukocyte trafficking in blood vessels and tissues. Curr Opin Immunol 2004; 16:406-17. PubMed
  • Williams CJ, Naito T, Arco PG, Seavitt JR, Cashman SM, De Souza B, Qi X, Keables P, Von Andrian UH, Georgopoulos K. The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development. Immunity 2004; 20:719-33. PubMed
  • Scimone ML, Felbinger TW, Mazo IB, Stein JV, Von Andrian UH, Weninger W. CXCL12 mediates CCR7-independent homing of central memory cells, but not naive T cells, in peripheral lymph nodes. J Exp Med 2004; 199:1113-20. PubMed
  • Salas A, Shimaoka M, Kogan AN, Harwood C, von Andrian UH, Springer TA. Rolling adhesion through an extended conformation of integrin alphaLbeta2 and relation to alpha I and beta I-like domain interaction. Immunity 2004; 20:393-406. PubMed
  • Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM. Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J Invest Dermatol 2004; 122:830-6. PubMed
  • Mempel TR, Henrickson SE, Von Andrian UH. T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases. Nature 2004; 427:154-9. PubMed
  • Rot A, von Andrian UH. Chemokines in innate and adaptive host defense: basic chemokinese grammar for immune cells. Annu Rev Immunol 2004; 22:891-928. PubMed
  • M'Rini C, Cheng G, Schweitzer C, Cavanagh LL, Palframan RT, Mempel TR, Warnock RA, Lowe JB, Quackenbush EJ, von Andrian UH. A novel endothelial L-selectin ligand activity in lymph node medulla that is regulated by alpha(1,3)-fucosyltransferase-IV. J Exp Med 2003; 198:1301-12. PubMed
  • van Zante A, Gauguet JM, Bistrup A, Tsay D, von Andrian UH, Rosen SD. Lymphocyte-HEV interactions in lymph nodes of a sulfotransferase-deficient mouse. J Exp Med 2003; 198:1289-300. PubMed
  • von Andrian UH, Mempel TR. Homing and cellular traffic in lymph nodes. Nat Rev Immunol 2003; 3:867-78. PubMed
  • Goodarzi K, Goodarzi M, Tager AM, Luster AD, von Andrian UH. Leukotriene B4 and BLT1 control cytotoxic effector T cell recruitment to inflamed tissues. Nat Immunol 2003; 4:965-73. PubMed
  • Weninger W, von Andrian UH. Chemokine regulation of nae T cell traffic in health and disease. Semin Immunol 2004; 15:257-70. PubMed
  • Martin CH, Aifantis I, Scimone ML, von Andrian UH, Reizis B, von Boehmer H, Gounari F. Efficient thymic immigration of B220+ lymphoid-restricted bone marrow cells with T precursor potential. Nat Immunol 2003; 4:866-73. PubMed
  • Wan J, Martinvalet D, Ji X, Lois C, Kaech SM, Von Andrian UH, Lieberman J, Ahmed R, Manjunath N. The Bcl-2 family pro-apoptotic molecule, BNIP3 regulates activation-induced cell death of effector cytotoxic T lymphocytes. Immunology 2003; 110:10-7. PubMed
  • Robert C, Klein C, Cheng G, Kogan A, Mulligan RC, von Andrian UH, Kupper TS. Gene therapy to target dendritic cells from blood to lymph nodes. Gene Ther 2003; 10:1479-86. PubMed
  • Mora JR, Bono MR, Manjunath N, Weninger W, Cavanagh LL, Rosemblatt M, Von Andrian UH. Selective imprinting of gut-homing T cells by Peyer's patch dendritic cells. Nature 2003; 424:88-93. PubMed
  • Weninger W, Carlsen HS, Goodarzi M, Moazed F, Crowley MA, Baekkevold ES, Cavanagh LL, von Andrian UH. Naive T cell recruitment to nonlymphoid tissues: a role for endothelium-expressed CC chemokine ligand 21 in autoimmune disease and lymphoid neogenesis. J Immunol 2003; 170:4638-48. PubMed
  • Wherry EJ, Teichgr. Lineage relationship and protective immunity of memory CD8 T cell subsets. Nat Immunol 2003; 4:225-34. PubMed
  • Wolf K, Mazo I, Leung H, Engelke K, von Andrian UH, Deryugina EI, Strongin AY, Br. Compensation mechanism in tumor cell migration: mesenchymal-amoeboid transition after blocking of pericellular proteolysis. J Cell Biol 2003; 160:267-77. PubMed
  • Hoffmeister KM, Felbinger TW, Falet H, Denis CV, Bergmeier W, Mayadas TN, von Andrian UH, Wagner DD, Stossel TP, Hartwig JH. The clearance mechanism of chilled blood platelets. Cell 2003; 112:87-97. PubMed
  • von Andrian UH, Engelhardt B. Alpha4 integrins as therapeutic targets in autoimmune disease. N Engl J Med 2003; 348:68-72. PubMed
  • Rom. CD4 effector T cell subsets in the response to influenza: heterogeneity, migration, and function. J Exp Med 2002; 196:957-68. PubMed
  • Cavanagh LL, Von Andrian UH. Travellers in many guises: the origins and destinations of dendritic cells. Immunol Cell Biol 2002; 80:448-62. PubMed
  • Weninger W, Manjunath N, von Andrian UH. Migration and differentiation of CD8+ T cells. Immunol Rev 2002; 186:221-33. PubMed
  • Mazo IB, Quackenbush EJ, Lowe JB, von Andrian UH. Total body irradiation causes profound changes in endothelial traffic molecules for hematopoietic progenitor cell recruitment to bone marrow. Blood 2002; 99:4182-91. PubMed
  • Lange-Asschenfeldt B, Weninger W, Velasco P, Kyriakides TR, von Andrian UH, Bornstein P, Detmar M. Increased and prolonged inflammation and angiogenesis in delayed-type hypersensitivity reactions elicited in the skin of thrombospondin-2--deficient mice. Blood 2002; 99:538-45. PubMed
  • Palframan RT, Jung S, Cheng G, Weninger W, Luo Y, Dorf M, Littman DR, Rollins BJ, Zweerink H, Rot A, von Andrian UH. Inflammatory chemokine transport and presentation in HEV: a remote control mechanism for monocyte recruitment to lymph nodes in inflamed tissues. J Exp Med 2001; 194:1361-73. PubMed
  • Weninger W, Crowley MA, Manjunath N, von Andrian UH. Migratory properties of naive, effector, and memory CD8(+) T cells. J Exp Med 2001; 194:953-66. PubMed
  • Fors BP, Goodarzi K, von Andrian UH. L-selectin shedding is independent of its subsurface structures and topographic distribution. J Immunol 2001; 167:3642-51. PubMed
  • Manjunath N, Shankar P, Wan J, Weninger W, Crowley MA, Hieshima K, Springer TA, Fan X, Shen H, Lieberman J, von Andrian UH. Effector differentiation is not prerequisite for generation of memory cytotoxic T lymphocytes. J Clin Invest 2001; 108:871-8. PubMed
  • Weiler H, Lindner V, Kerlin B, Isermann BH, Hendrickson SB, Cooley BC, Meh DA, Mosesson MW, Shworak NW, Post MJ, Conway EM, Ulfman LH, von Andrian UH, Weitz JI. Characterization of a mouse model for thrombomodulin deficiency. Arterioscler Thromb Vasc Biol 2001; 21:1531-7. PubMed
  • Homeister JW, Thall AD, Petryniak B, Mal. The alpha(1,3)fucosyltransferases FucT-IV and FucT-VII exert collaborative control over selectin-dependent leukocyte recruitment and lymphocyte homing. Immunity 2001; 15:115-26. PubMed
  • von Andrian UH. PKC-beta(I): the whole ignition system or just a sparkplug for T cell migration? Nat Immunol 2001; 2:477-8. PubMed
  • Shimaoka M, Lu C, Palframan RT, von Andrian UH, McCormack A, Takagi J, Springer TA. Reversibly locking a protein fold in an active conformation with a disulfide bond: integrin alphaL I domains with high affinity and antagonist activity in vivo. Proc Natl Acad Sci U S A 2001; 98:6009-14. PubMed
  • Baekkevold ES, Yamanaka T, Palframan RT, Carlsen HS, Reinholt FP, von Andrian UH, Brandtzaeg P, Haraldsen G. The CCR7 ligand elc (CCL19) is transcytosed in high endothelial venules and mediates T cell recruitment. J Exp Med 2001; 193:1105-12. PubMed
  • Mackay CR, von Andrian UH. Immunology. Memory T cells--local heroes in the struggle for immunity. Science 2001; 291:2323-4. PubMed
  • von Andrian UH, Mackay CR. T-cell function and migration. Two sides of the same coin. N Engl J Med 2000; 343:1020-34. PubMed
  • Tager AM, Dufour JH, Goodarzi K, Bercury SD, von Andrian UH, Luster AD. BLTR mediates leukotriene B(4)-induced chemotaxis and adhesion and plays a dominant role in eosinophil accumulation in a murine model of peritonitis. J Exp Med 2000; 192:439-46. PubMed
  • Weninger W, Ulfman LH, Cheng G, Souchkova N, Quackenbush EJ, Lowe JB, von Andrian UH. Specialized contributions by alpha(1,3)-fucosyltransferase-IV and FucT-VII during leukocyte rolling in dermal microvessels. Immunity 2000; 12:665-76. PubMed
  • Stein JV, Rot A, Luo Y, Narasimhaswamy M, Nakano H, Gunn MD, Matsuzawa A, Quackenbush EJ, Dorf ME, von Andrian UH. The CC chemokine thymus-derived chemotactic agent 4 (TCA-4, secondary lymphoid tissue chemokine, 6Ckine, exodus-2) triggers lymphocyte function-associated antigen 1-mediated arrest of rolling T lymphocytes in peripheral lymph node high endothelial venules J Exp Med 2000; 191:61-76. PubMed
  • Salgia R, Quackenbush E, Lin J, Souchkova N, Sattler M, Ewaniuk DS, Klucher KM, Daley GQ, Kraeft SK, Sackstein R, Alyea EP, von Andrian UH, Chen LB, Gutierrez-Ramos JC, Pendergast AM, Griffin JD. The BCR/ABL oncogene alters the chemotactic response to stromal-derived factor-1alpha. Blood 1999; 94:4233-46. PubMed
  • Manjunath N, Shankar P, Stockton B, Dubey PD, Lieberman J, von Andrian UH. A transgenic mouse model to analyze CD8(+) effector T cell differentiation in vivo. Proc Natl Acad Sci U S A 1999; 96:13932-7. PubMed
  • Mazo IB, von Andrian UH. Adhesion and homing of blood-borne cells in bone marrow microvessels. J Leukoc Biol 1999; 66:25-32. PubMed
  • Robert C, Fuhlbrigge RC, Kieffer JD, Ayehunie S, Hynes RO, Cheng G, Grabbe S, von Andrian UH, Kupper TS. Interaction of dendritic cells with skin endothelium: A new perspective on immunosurveillance. J Exp Med 1999; 189:627-36. PubMed
  • Stein JV, Cheng G, Stockton BM, Fors BP, Butcher EC, von Andrian UH. L-selectin-mediated leukocyte adhesion in vivo: microvillous distribution determines tethering efficiency, but not rolling velocity. J Exp Med 1999; 189:37-50. PubMed
  • Frenette PS, Subbarao S, Mazo IB, von Andrian UH, Wagner DD. Endothelial selectins and vascular cell adhesion molecule-1 promote hematopoietic progenitor homing to bone marrow. Proc Natl Acad Sci U S A 1998; 95:14423-8. PubMed
  • Mazo IB, Gutierrez-Ramos JC, Frenette PS, Hynes RO, Wagner DD, von Andrian UH. Hematopoietic progenitor cell rolling in bone marrow microvessels: parallel contributions by endothelial selectins and vascular cell adhesion molecule 1. J Exp Med 1998; 188:465-74. PubMed
  • Stockton BM, Cheng G, Manjunath N, Ardman B, von Andrian UH. Negative regulation of T cell homing by CD43. Immunity 1998; 8:373-81. PubMed
  • Diacovo TG, Catalina MD, Siegelman MH, von Andrian UH. Circulating activated platelets reconstitute lymphocyte homing and immunity in L-selectin-deficient mice. J Exp Med 1998; 187:197-204. PubMed
  • Warnock RA, Askari S, Butcher EC, von Andrian UH. Molecular mechanisms of lymphocyte homing to peripheral lymph nodes. J Exp Med 1998; 187:205-16. PubMed
  • von Andrian UH, M'Rini C. In situ analysis of lymphocyte migration to lymph nodes. Cell Adhes Commun 1998; 6:85-96. PubMed
  • von Andrian UH. A massage for the journey: keeping leukocytes soft and silent. Proc Natl Acad Sci U S A 1997; 94:4825-7. PubMed
  • Thiel M, Zourelidis C, Chambers JD, von Andrian UH, Arfors KE, Messmer K, Peter K. Expression of beta 2-integrins and L-selectin on polymorphonuclear leukocytes in septic patients. Eur Surg Res 1997; 29:160-75. PubMed
  • Stoddart JH, Jasuja RR, Sikorski MA, von Andrian UH, Mier JW. Protease-resistant L-selectin mutants. Down-modulation by cross-linking but not cellular activation. J Immunol 1996; 157:5653-9. PubMed
  • Coxon A, Rieu P, Barkalow FJ, Askari S, Sharpe AH, von Andrian UH, Arnaout MA, Mayadas TN. A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation. Immunity 1996; 5:653-66. PubMed
  • von Andrian UH. Intravital microscopy of the peripheral lymph node microcirculation in mice. Microcirculation 1996; 3:287-300. PubMed
  • Mal. The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis. Cell 1996; 86:643-53. PubMed
  • Diacovo TG, Puri KD, Warnock RA, Springer TA, von Andrian UH. Platelet-mediated lymphocyte delivery to high endothelial venules. Science 1996; 273:252-5. PubMed
  • Finger EB, Puri KD, Alon R, Lawrence MB, von Andrian UH, Springer TA. Adhesion through L-selectin requires a threshold hydrodynamic shear. Nature 1996; 379:266-9. PubMed
  • von Andrian UH, Hasslen SR, Nelson RD, Erlandsen SL, Butcher EC. A central role for microvillous receptor presentation in leukocyte adhesion under flow. Cell 1995; 82:989-99. PubMed
  • Hasslen SR, von Andrian UH, Butcher EC, Nelson RD, Erlandsen SL. Spatial distribution of L-selectin (CD62L) on human lymphocytes and transfected murine L1-2 cells. Histochem J 1995; 27:547-54. PubMed
  • Berlin C, Bargatze RF, Campbell JJ, von Andrian UH, Szabo MC, Hasslen SR, Nelson RD, Berg EL, Erlandsen SL, Butcher EC. alpha 4 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 1995; 80:413-22. PubMed
  • Lehr HA, Krombach F, M. In vitro effects of oxidized low density lipoprotein on CD11b/CD18 and L-selectin presentation on neutrophils and monocytes with relevance for the in vivo situation. Am J Pathol 1995; 146:218-27. PubMed
  • Sriramarao P, von Andrian UH, Butcher EC, Bourdon MA, Broide DH. L-selectin and very late antigen-4 integrin promote eosinophil rolling at physiological shear rates in vivo. J Immunol 1994; 153:4238-46. PubMed
  • Olofsson AM, Arfors KE, Ramezani L, Wolitzky BA, Butcher EC, von Andrian UH. E-selectin mediates leukocyte rolling in interleukin-1-treated rabbit mesentery venules. Blood 1994; 84:2749-58. PubMed
  • Lehr HA, Olofsson AM, Carew TE, Vajkoczy P, von Andrian UH, H. P-selectin mediates the interaction of circulating leukocytes with platelets and microvascular endothelium in response to oxidized lipoprotein in vivo. Lab Invest 1994; 71:380-6. PubMed
  • von Andrian UH, Chambers JD, Berg EL, Michie SA, Brown DA, Karolak D, Ramezani L, Berger EM, Arfors KE, Butcher EC. L-selectin mediates neutrophil rolling in inflamed venules through sialyl LewisX-dependent and -independent recognition pathways. Blood 1993; 82:182-91. PubMed
  • von Andrian UH, Berger EM, Ramezani L, Chambers JD, Ochs HD, Harlan JM, Paulson JC, Etzioni A, Arfors KE. In vivo behavior of neutrophils from two patients with distinct inherited leukocyte adhesion deficiency syndromes. J Clin Invest 1993; 91:2893-7. PubMed
  • von Andrian UH, Arfors KE. Neutrophil-endothelial cell interactions in vivo: a chain of events characterized by distinct molecular mechanisms. Agents Actions Suppl 1993; 41:153-64. PubMed
  • Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE. L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol 1992; 263:H1034-44. PubMed
  • Rosengren S, Olofsson AM, von Andrian UH, Lundgren-Akerlund E, Arfors KE. Leukotriene B4-induced neutrophil-mediated endothelial leakage in vitro and in vivo. J Appl Physiol 1991; 71:1322-30. PubMed
  • von Andrian UH, Chambers JD, McEvoy LM, Bargatze RF, Arfors KE, Butcher EC. Two-step model of leukocyte-endothelial cell interaction in inflammation: distinct roles for LECAM-1 and the leukocyte beta 2 integrins in vivo. Proc Natl Acad Sci U S A 1991; 88:7538-42. PubMed
  • Kempski O, von Andrian U, Sch. Intravenous glutamate enhances edema formation after a freezing lesion. Adv Neurol 1990; 52:219-23. PubMed
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