Timothy J. Mitchison, PhD

Harvard Medical School

Harvard Medical School
Phone: (617) 432-3805
Fax: (617) 432-3702


timothy_mitchison@hms.harvard.edu

Timothy J. Mitchison, PhD

Harvard Medical School

EDUCATIONAL TITLES

  • Hasib Sabbagh Professor of Systems Biology, Systems Biology, Harvard Medical School
  • Deputy Chairman, Systems Biology, Harvard Medical School

DF/HCC PROGRAM AFFILIATION

Publications

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  • Al-Obaidi N, Mitchison TJ, Crews CM, Mayer TU. Identification of MAC1: A Small Molecule That Rescues Spindle Bipolarity in Monastrol-Treated Cells. ACS Chem Biol 2016. PubMed
  • Florian S, Mitchison TJ. Anti-Microtubule Drugs. Methods Mol Biol 2016; 1413:403-21. PubMed
  • Groen AC, Mitchison TJ. Purification and Fluorescent Labeling of Tubulin from Xenopus laevis Egg Extracts. Methods Mol Biol 2016; 1413:35-45. PubMed
  • Son S, Kang JH, Oh S, Kirschner MW, Mitchison TJ, Manalis S. Resonant microchannel volume and mass measurements show that suspended cells swell during mitosis. J Cell Biol 2015; 211:757-63. PubMed
  • Kuhn H, Sopko R, Coughlin M, Perrimon N, Mitchison T. The Atg1-Tor pathway regulates yolk catabolism in Drosophila embryos. Development 2015. PubMed
  • Wühr M, Güttler T, Peshkin L, McAlister GC, Sonnett M, Ishihara K, Groen AC, Presler M, Erickson BK, Mitchison TJ, Kirschner MW, Gygi SP. The Nuclear Proteome of a Vertebrate. Curr Biol 2015. PubMed
  • Field CM, Groen AC, Nguyen PA, Mitchison TJ. Spindle-to-cortex communication in cleaving, polyspermic Xenopus eggs. Mol Biol Cell 2015. PubMed
  • Li L, Yin Q, Kuss P, Maliga Z, Millán JL, Wu H, Mitchison TJ. Erratum: Hydrolysis of 2'3'-cGAMP by ENPP1 and design of nonhydrolyzable analogs. Nat Chem Biol 2015; 11:741. PubMed
  • Chittajallu DR, Florian S, Kohler RH, Iwamoto Y, Orth JD, Weissleder R, Danuser G, Mitchison TJ. In vivo cell-cycle profiling in xenograft tumors by quantitative intravital microscopy. Nat Methods 2015; 12:577-85. PubMed
  • Krukenberg KA, Kim S, Tan ES, Maliga Z, Mitchison TJ. Extracellular poly(ADP-ribose) is a pro-inflammatory signal for macrophages. Chem Biol 2015; 22:446-52. PubMed
  • Özlü N, Qureshi MH, Toyoda Y, Renard BY, Mollaoglu G, Özkan NE, Bulbul S, Poser I, Timm W, Hyman AA, Mitchison TJ, Steen JA. Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis. EMBO J 2015; 34:251-65. PubMed
  • Nguyen PA, Field CM, Groen AC, Mitchison TJ, Loose M. Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins. Methods Cell Biol. 2015; 128:223-41. PubMed
  • Mitchison TJ, Ishihara K, Nguyen P, Wühr M. Size Scaling of Microtubule Assemblies in Early Xenopus Embryos. Cold Spring Harb Perspect Biol 2015. PubMed
  • Ishihara K, Nguyen PA, Groen AC, Field CM, Mitchison TJ. Microtubule nucleation remote from centrosomes may explain how asters span large cells. Proc Natl Acad Sci U S A 2014; 111:17715-22. PubMed
  • Li L, Yin Q, Kuss P, Maliga Z, Millán JL, Wu H, Mitchison TJ. Hydrolysis of 2'3'-cGAMP by ENPP1 and design of nonhydrolyzable analogs. Nat Chem Biol 2014; 10:1043-8. PubMed
  • Choi PJ, Mitchison TJ. Quantitative analysis of resistance to natural killer attacks reveals stepwise killing kinetics. Integr Biol (Camb) 2014. PubMed
  • Laughney AM, Kim E, Sprachman MM, Miller MA, Kohler RH, Yang KS, Orth JD, Mitchison TJ, Weissleder R. Single-cell pharmacokinetic imaging reveals a therapeutic strategy to overcome drug resistance to the microtubule inhibitor eribulin. Sci Transl Med 2014; 6:261ra152. PubMed
  • Nguyen PA, Groen AC, Loose M, Ishihara K, Wühr M, Field CM, Mitchison TJ. Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Science 2014; 346:244-7. PubMed
  • Krukenberg KA, Jiang R, Steen JA, Mitchison TJ. Basal activity of a PARP1-NuA4 complex varies dramatically across cancer cell lines. Cell Rep 2014; 8:1808-18. PubMed
  • Ishihara K, Nguyen PA, Wühr M, Groen AC, Field CM, Mitchison TJ. Organization of early frog embryos by chemical waves emanating from centrosomes. Philos Trans R Soc Lond B Biol Sci 2014. PubMed
  • Field CM, Nguyen PA, Ishihara K, Groen AC, Mitchison TJ. Xenopus egg cytoplasm with intact actin. Methods Enzymol 2014; 540:399-415. PubMed
  • Groen AC, Ngyuen PA, Field CM, Ishihara K, Mitchison TJ. Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization. Methods Enzymol 2014; 540:417-33. PubMed
  • Wang Y, Lee YM, Baitsch L, Huang A, Xiang Y, Tong H, Lako A, Von T, Choi C, Lim E, Min J, Li L, Stegmeier F, Schlegel R, Eck MJ, Gray NS, Mitchison TJ, Zhao JJ. MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells. Elife 2014. PubMed
  • Coughlin M, Groen AC, Mitchison TJ. Electron microscopy of microtubule cytoskeleton assembly in vitro. Methods Mol Biol 2013; 1117:259-71. PubMed
  • Prentice-Mott HV, Chang CH, Mahadevan L, Mitchison TJ, Irimia D, Shah JV. Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments. Proc Natl Acad Sci U S A 2013; 110:21006-11. PubMed
  • Tang Y, Xie T, Florian S, Moerke N, Shamu C, Benes C, Mitchison TJ. Differential determinants of cancer cell insensitivity to antimitotic drugs discriminated by a one-step cell imaging assay. J Biomol Screen 2013; 18:1062-71. PubMed
  • Zidovska A, Weitz DA, Mitchison TJ. Micron-scale coherence in interphase chromatin dynamics. Proc Natl Acad Sci U S A 2013; 110:15555-60. PubMed
  • Barkauskaite E, Brassington A, Tan ES, Warwicker J, Dunstan MS, Banos B, Lafite P, Ahel M, Mitchison TJ, Ahel I, Leys D. Visualization of poly(ADP-ribose) bound to PARG reveals inherent balance between exo- and endo-glycohydrolase activities. Nat Commun 2013; 4:2164. PubMed
  • Thurber GM, Yang KS, Reiner T, Kohler RH, Sorger P, Mitchison T, Weissleder R. Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo. Nat Commun 2013; 4:1504. PubMed
  • Tan ES, Krukenberg KA, Mitchison TJ. Large-scale preparation and characterization of poly(ADP-ribose) and defined length polymers. Anal Biochem 2012; 428:126-36. PubMed
  • Zhang X, Wang W, Bedigian AV, Coughlin ML, Mitchison TJ, Eggert US. Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI. Proc Natl Acad Sci U S A 2012; 109:12485-90. PubMed
  • Dumont S, Salmon ED, Mitchison TJ. Deformations within moving kinetochores reveal different sites of active and passive force generation. Science 2012; 337:355-8. PubMed
  • Hu CK, Ozlü N, Coughlin M, Steen JJ, Mitchison TJ. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 2012; 23:2702-11. PubMed
  • Kim S, Peshkin L, Mitchison TJ. Vascular disrupting agent drug classes differ in effects on the cytoskeleton. PLoS ONE 2012; 7:e40177. PubMed
  • Wühr M, Tan ES, Parker SK, Detrich HW, Mitchison TJ. A model for cleavage plane determination in early amphibian and fish embryos. Curr Biol 2010; 20:2040-5. PubMed
  • Mitchison TJ. Remaining mysteries of the cytoplasm. Mol Biol Cell 2010; 21:3811-2. PubMed
  • Kueh HY, Brieher WM, Mitchison TJ. Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover. Biophys J 2010; 99:2153-62. PubMed
  • Huang HC, Shi J, Orth JD, Mitchison TJ. Cell death when the SAC is out of commission. Cell Cycle 2010. PubMed
  • Martin DS, Fathi R, Mitchison TJ, Gelles J. FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry. Proc Natl Acad Sci U S A 2010; 107:5453-8. PubMed
  • Gatlin JC, Matov A, Danuser G, Mitchison TJ, Salmon ED. Directly probing the mechanical properties of the spindle and its matrix. J Cell Biol 2010; 188:481-9. PubMed
  • Ozlü N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ. Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition. Mol Cell Proteomics 2010; 9:336-50. PubMed
  • Huang HC, Mitchison TJ, Shi J. Stochastic competition between mechanistically independent slippage and death pathways determines cell fate during mitotic arrest. PLoS ONE 2011; 5:e15724. PubMed
  • Chang P, Coughlin M, Mitchison TJ. Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly. Mol Biol Cell 2009; 20:4575-85. PubMed
  • Huang HC, Shi J, Orth JD, Mitchison TJ. Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly. Cancer Cell 2009; 16:347-58. PubMed
  • Dumont S, Mitchison TJ. Force and length in the mitotic spindle. Curr Biol 2009; 19:R749-61. PubMed
  • Charras GT, Mitchison TJ, Mahadevan L. Animal cell hydraulics. J Cell Sci 2009; 122:3233-41. PubMed
  • Kueh HY, Mitchison TJ. Structural plasticity in actin and tubulin polymer dynamics. Science 2009; 325:960-3. PubMed
  • Maresca TJ, Groen AC, Gatlin JC, Ohi R, Mitchison TJ, Salmon ED. Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity. Curr Biol 2009; 19:1210-5. PubMed
  • Dumont S, Mitchison TJ. Compression regulates mitotic spindle length by a mechanochemical switch at the poles. Curr Biol 2009; 19:1086-95. PubMed
  • Feng Y, Mitchison TJ, Bender A, Young DW, Tallarico JA. Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds. Nat Rev Drug Discov 2009; 8:567-78. PubMed
  • Niethammer P, Grabher C, Look AT, Mitchison TJ. A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature 2009; 459:996-9. PubMed
  • Needleman DJ, Xu Y, Mitchison TJ. Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy. Biophys J 2009; 96:5050-9. PubMed
  • Groen AC,Maresca TJ,Gatlin JC,Salmon ED,Mitchison TJ. Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly. Mol Biol Cell 2009; 20:2766-73. PubMed
  • Gatlin JC,Matov A,Groen AC,Needleman DJ,Maresca TJ,Danuser G,Mitchison TJ,Salmon ED. Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules. Curr Biol 2009; 19:287-96. PubMed
  • Tsui M, Xie T, Orth JD, Carpenter AE, Rudnicki S, Kim S, Shamu CE, Mitchison TJ. An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor. PLoS ONE 2009; 4:e7339. PubMed
  • Orth JD,Tang Y,Shi J,Loy CT,Amendt C,Wilm C,Zenke FT,Mitchison TJ. Quantitative live imaging of cancer and normal cells treated with Kinesin-5 inhibitors indicates significant differences in phenotypic responses and cell fate. Mol Cancer Ther 2008; 7:3480-9. PubMed
  • Maloney KN,Fujita M,Eggert US,Schroeder FC,Field CM,Mitchison TJ,Clardy J. Actin-Aggregating Cucurbitacins from Physocarpus capitatus. J Nat Prod 2008. PubMed
  • Kueh HY,Brieher WM,Mitchison TJ. Dynamic stabilization of actin filaments. Proc Natl Acad Sci U S A 2008; 105:16531-6. PubMed
  • Wuhr M,Mitchison TJ,Field CM. Mitosis: new roles for myosin-X and actin at the spindle. Curr Biol 2008; 18:R912-4. PubMed
  • Wuhr M,Chen Y,Dumont S,Groen AC,Needleman DJ,Salic A,Mitchison TJ. Evidence for an upper limit to mitotic spindle length. Curr Biol 2008; 18:1256-61. PubMed
  • Kueh HY,Charras GT,Mitchison TJ,Brieher WM. Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. J Cell Biol 2008; 182:341-53. PubMed
  • Groen AC,Needleman D,Brangwynne C,Gradinaru C,Fowler B,Mazitschek R,Mitchison TJ. A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly. J Cell Sci 2008; 121:2293-300. PubMed
  • Mitchison TJ, Charras GT, Mahadevan L. Implications of a poroelastic cytoplasm for the dynamics of animal cell shape. Semin Cell Dev Biol 2008; 19:215-23. PubMed
  • Niethammer P, Kueh HY, Mitchison TJ. Spatial patterning of metabolism by mitochondria, oxygen, and energy sinks in a model cytoplasm. Curr Biol 2008; 18:586-91. PubMed
  • Hu CK, Coughlin M, Field CM, Mitchison TJ. Cell polarization during monopolar cytokinesis. J Cell Biol 2008; 181:195-202. PubMed
  • Charras GT, Coughlin M, Mitchison TJ, Mahadevan L. Life and times of a cellular bleb. Biophys J 2007; 94:1836-53. PubMed
  • Galkin VE, Orlova A, Brieher W, Kueh HY, Mitchison TJ, Egelman EH. Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament. J Mol Biol 2008; 376:607-13. PubMed
  • Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A 2008; 105:2415-20. PubMed
  • Young DW, Bender A, Hoyt J, McWhinnie E, Chirn GW, Tao CY, Tallarico JA, Labow M, Jenkins JL, Mitchison TJ, Feng Y. Integrating high-content screening and ligand-target prediction to identify mechanism of action. Nat Chem Biol 2007; 4:59-68. PubMed
  • Irimia D, Charras G, Agrawal N, Mitchison T, Toner M. Polar stimulation and constrained cell migration in microfluidic channels. Lab Chip 2007; 7:1783-90. PubMed
  • Vrabioiu AM, Mitchison TJ. Symmetry of septin hourglass and ring structures. J Mol Biol 2007; 372:37-49. PubMed
  • Burbank KS, Mitchison TJ, Fisher DS. Slide-and-cluster models for spindle assembly. Curr Biol 2007; 17:1373-83. PubMed
  • Ohi R, Burbank K, Liu Q, Mitchison TJ. Nonredundant functions of Kinesin-13s during meiotic spindle assembly. Curr Biol 2007; 17:953-9. PubMed
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