John Asara Ph.D.

Instructor, Department of Pathology, Harvard Medical School

Director, Mass Spectrometry Core , Beth Israel Deaconess Medical Center

Contact Info

John Asara
Beth Israel Deaconess Medical Center
77 Avenue Louis Pasteur
Boston, MA, 02115
Mailstop:
Phone: 6176676182
Fax:
jasara@bidmc.harvard.edu

DF/HCC Program Affiliation

Publications

• Asara JM, Schweitzer MH, Freimark LM, Phillips M, Cantley LC. Protein Sequences from Mastodon and Tyrannosaurus Rex Revealed by Mass Spectrometry. Science 2007 Apr 13; 316(5822):280-285
  PMID: 17431180
• Schweitzer MH, Suo Z, Avci R, Asara JM, Allen MA, Arce FT, Horner JR. Analyses of soft tissue from Tyrannosaurus rex suggest the presence of protein. Science 2007 Apr 13; 316(5822):277-80
  PMID: 17431179
• Asara JM, Zhang X, Zheng B, Maroney LA, Christofk HR, Wu N, Cantley LC. In-gel stable isotope labeling for relative quantification using mass spectrometry. Nat Protoc 2006; 1(1):46-51
  PMID: 17406210
• Asara JM, Zhang X, Zheng B, Christofk HH, Wu N, Cantley LC. In-Gel Stable-Isotope Labeling (ISIL): a strategy for mass spectrometry-based relative quantification. J Proteome Res 2006 Jan; 5(1):155-63
  PMID: 16396506
• Abbott DW, Wilkins A, Asara JM, Cantley LC. The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site on NEMO. Curr Biol 2004 Dec 29; 14(24):2217-27
  PMID: 15620648
• Kane S, Sano H, Liu SC, Asara JM, Lane WS, Garner CC, Lienhard GE. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. J Biol Chem 2002 Jun 21; 277(25):22115-8
  PMID: 11994271
• Ivan M, Kondo K, Yang H, Kim W, Valiando J, Ohh M, Salic A, Asara JM, Lane WS, Kaelin WG. HIFalpha targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science 2001 Apr 20; 292(5516):464-8
  PMID: 11292862
• Asara JM, Allison J. Enhanced detection of phosphopeptides in matrix-assisted laser desorption/ionization mass spectrometry using ammonium salts. J Am Soc Mass Spectrom 1999 Jan; 10(1):35-44
  PMID: 9888183