DF/HCC Program Affiliation

Cancer Genetics
Cancer Cell Biology

Research Abstract

Our laboratory uses Drosophila as a model system to characterize evolutionary conserved signaling transduction pathways. Our current studies focus on the analysis of Wnt, EGF Receptor, and JAK/STAT signaling transduction pathways. In addition, other projects in the lab focus on the characterization of signaling pathways that regulate the establishment of polarity and cytoskeletal organization.

Publications

• Lu J, Ruhf ML, Perrimon N, Leder P.A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression.Proc Natl Acad Sci U S A 2007 May 29;104(22):9381-6.
  17517653
• Mathey-Prevot B, Perrimon N.Do-it-yourself RNAi made easy?.Nat Methods 2007 Apr;4(4):308-9.
  17396126
• Mathey-Prevot B, Perrimon N.Drosophila genome-wide RNAi screens: are they delivering the promise?.Cold Spring Harb Symp Quant Biol 2006;71:141-8.
  17381290
• Friedman A, Perrimon N.Genetic screening for signal transduction in the era of network biology.Cell 2007 Jan 26;128(2):225-31.
  17254958
• Perrimon N, Mathey-Prevot B.Applications of high-throughput RNA interference screens to problems in cell and developmental biology.Genetics 2007 Jan;175(1):7-16.
  17244760
• Perrimon N, Friedman A, Mathey-Prevot B, Eggert US.Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens.Drug Discov Today 2007 Jan;12(1-2):28-33.
  17198970
• Friedman A, Perrimon N.A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.Nature 2006 Nov 9;444(7116):230-4.
  17086199
• Bach EA, Ekas LA, Ayala-Camargo A, Flaherty MS, Lee H, Perrimon N, Baeg GH.GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo.Gene Expr Patterns 2007 Jan;7(3):323-31.
  17008134
• Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG, Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L, Ma Y, Mathey-Prévôt B, Root DE, Sabatini DM, Taipale J, Perrimon N, Bernards R.Minimizing th
  16990807
• Kulkarni MM, Booker M, Silver SJ, Friedman A, Hong P, Perrimon N, Mathey-Prevot B.Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.Nat Methods 2006 Oct;3(10):833-8.
  16964256
• Gibson MC, Patel AB, Nagpal R, Perrimon N.The emergence of geometric order in proliferating metazoan epithelia.Nature 2006 Aug 31;442(7106):1038-41.
  16900102
• Friedman A, Perrimon N.High-throughput approaches to dissecting MAPK signaling pathways.Methods 2006 Jul 14.
  16844385
• Chen LY, Wang JC, Hyvert Y, Lin HP, Perrimon N, Imler JL, Hsu JC.Weckle is a zinc finger adaptor of the toll pathway in dorsoventral patterning of the Drosophila embryo.Curr Biol 2006 Jun 20;16(12):1183-93.
  16782008
• Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N, Malhotra V.Functional genomics reveals genes involved in protein secretion and Golgi organization.Nature 2006 Feb 2;439(7076):604-7.
  16452979
• Flockhart I, Booker M, Kiger A, Boutros M, Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N, Mathey-Prevot B.FlyRNAi: the Drosophila RNAi screening center database.Nucleic Acids Res 2006 Jan 1;34(Database issue):D489-94.
  16381918
• Micchelli CA, Perrimon N.Evidence that stem cells reside in the adult Drosophila midgut epithelium.Nature 2006 Jan 26;439(7075):475-9.
  16340959
• Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N.A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.Nat Genet 2005 Dec;37(12):1323-32.
  16311596
• Karsten P, Plischke I, Perrimon N, Zeidler MP.Mutational analysis reveals separable DNA binding and trans-activation of Drosophila STAT92E.Cell Signal 2006 Jun;18(6):819-29.
  16129580
• Ghiglione C, Carraway KL, Amundadottir LT, Boswell RE, Perrimon N, Duffy JB.The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis.Cell 1999 Mar 19;96(6):847-56.
  10102272