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Frederick W. Alt, PhD

Charles A. Janeway Professor of Pediatrics, Department of Pediatrics, Harvard Medical School

Professor, Department of Genetics, Harvard Medical School

Investigator, Howard Hughes Medical Institute, Boston Children's Hospital

Contact Info

Frederick Alt
Boston Children's Hospital
300 Longwood Avenue
Boston, MA, 02115
Mailstop: Karp Bld. Rm 09216
Phone: 617-919-2539
Fax: 617-730-0948
Alt@enders.tch.harvard.edu

Assistant

Judy Needham
Executive Assistant
Program In Cellular and Molecular Medicine
Children's Hospital
300 Longwood Avenue
Karp Building 09215.1
Boston, MA, 02115
Phone: 617-919-2539
Fax: 617-730-0948
judith.needham@harvard.childrens.edu
Kristine King
Administrative Assistant
Howard Hughes Medical Institute
300 Longwood Avenue
Karp Building-- RM 09216.1
Boston, MA, 02115
Phone: 617-919-2539
Fax: 617-730-0948
kristine.king@harvard.childrens.edu

DF/HCC Program Affiliation

Lymphoma and Myeloma
Cancer Immunology

Lab Website

Alt Lab

Research Abstract

The broad focus of the Alt lab is the elucidation of mechanisms that generate antigen receptor diversity in the Immune system and mechanisms that maintain genomic stability in mammalian cells. More specifically, the lab studies V(D)J recombination in developing B and T lymphocytes and IgH heavy chain class switch recombination (CSR) and somatic hypermutation in mature B lymphocytes. Studies of these processes continue to employ biochemical, molecular and mouse modeling approaches to elucidate mechanisms by which the RAG endonuclease and Activation Induced Cytidine Deaminase function on DNA to initiate, respectively, VDJ recombination and CSR. One focus of this work is to elucidate how the DNA lesion generating activities of these lymphocyte-specific enzymes is directed to specific antigen receptor locus substrates and not to off targets that could generate chromosomal translocations and deletions that contribute to lymphoid cancers. Other studies are aimed at elucidating roles of general DNA double strand break (DSB) repair and response pathways in VDJ recombination and CSR, and the interplay of DSB repair and response pathways in suppressing genomic instability and cancer. A major new lab research area focuses on how organization of the genome in the nucleus influences programmed gene rearrangements and chromosomal translocations. For this purpose, the lab developed a high throughput genomic translocation sequencing (HTGTS) to identify the translocations genome wide that arise from fixed DSBs. This approach also identifies sites of endogenous genomic DSBs including RAG or AID off-target sequences and transcription start sites. Ongoing work seeks to elucidate mechanistic elements (three dimensional genome organization, DSBs, transcription, epigenetic modifications, repair pathways, etc) that contribute to the formation of translocations and other forms of genomic instability in mouse and human cancer cells.

Publications

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