DF/HCC Program Affiliation

Cancer Cell Biology
Cancer Genetics

Research Abstract

I am interested in a number of areas relevant to cancer biology. I have had extensive experience in unraveling the signaling pathways involved in sensing and responding to DNA damage. This has involved both yeast and mammalian systems. We have performed work on the yeast Mec1, Mrc1, Rad53, Chk1, Dun1, Crt1, RNR1, RNR2, RNR3 and RNR4 genes as well as a number of cell cycle regulators including Cdc5 and the mitotic exit network genes Bub1, Bfa1 and Amn1. In mammals we have worked on the ATM, ATR,ATRIP, RPA, Rad17, Claspin, Chk1, Chk2,Cdc25 and p53 genes. I am continuing to explore new avenues in DNA damage signaling. In addiiton to our interests in DNA damage and cell cycle regulation in general, I have worked extensively in the past on Cdk regulation including Cdk2, cyclin A, cyclin F, and the p21 family of Cdk inhibitors, CKIs, including the Beckwith-Weidemann gene, p57. More recently our studies of cyclin and CKI metabolism led to the discovery of F-box proteins and the SCF pathway of regulated protein stability. Our more recent studies have focussed on development of genetic methods to over-express and knock-out mammalian genes using siRNA to perform genetic screens in mammalian cells.

Publications

View All Publications