John P. Manis, MD
Assistant Professor, Department of Pathology, Harvard Medical School
Investigator, Joint Program in Transfusion Medicine, Boston Children's Hospital
DF/HCC Program AffiliationLymphoma and Myeloma
Our lab focuses on the response to and repair of programmed DNA breaks that occur during B cell development. B cells undergo programmed DNA breaks during a normal immune response in order to generate higher affinity antibodies with specialized functions. Immunoglobulin class switch recombination (CSR) alters the effector function of antibodies without a change in antigen specificity, emlpoying a process that requires DNA double strand breaks. Errors in the repair of these programmed double strand breaks required for CSR are thought to result in oncogenic translocations commonly seen in human B cell lymphoma. We seek to elucidate the role of the DNA damage response (DDR) pathway in the development of germinal center B cell lymphoma, focusing on members of the DDR pathway including ATM, 53BP1, and H2AX. The DDR pathway is triggered early after DNA damage and is found to be activated during the intial phases of carcinogenesis. By examining the DDR both in normal human B cell populations within a germinal center reaction, and in lymphoma samples, we can gain valuable insights into the alterations which predispose to lymphomagenesis and additionally allow for the development of clinically useful diagnostic and prognostic tools.
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