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Donald W. Kufe, MD

Professor, Department of Medicine, Harvard Medical School

Leader, Translational Pharmacology and Early Therapeutic Trials Program, Dana-Farber/Harvard Cancer Center

Contact Info

Donald Kufe
Dana-Farber Cancer Institute
450 Brookline Avenue
Boston, MA, 02215
Mailstop: Dana 830
Phone: 617-632-3141
Fax: 617-632-2934
donald_kufe@dfci.harvard.edu

Assistant

Mary Werowinski
Medical Oncology
Dana-Farber Cancer Institute
450 Brookline Avenue
Dana 830C
Boston, MA, 02215
Mailstop: D830C
Phone: 617-632-5614
Fax: 617-632-2934
mary_werowinski@dfci.harvard.edu

DF/HCC Program Affiliation

Kidney Cancer
Translational Pharmacology and Early Therapeutic Trials, Leader

DF/HCC Associations

Member, Center Scientific Council

Research Abstract

The Kufe laboratory identified the human DF3/MUC1 carcinoma-associated protein in the early 1980s. Initial studies demonstrated that MUC1 is aberrantly overexpressed in >90% of human breast cancers. Subsequent work by multiple laboratories showed that MUC1 is expressed at elevated levels by diverse carcinomas and certain hematologic malignancies, including multiple myeloma. Estimates indicate that MUC1 is overexpressed in about 900,000 of the 1.4 million tumors diagnosed each year in the United States.

MUC1 is translated as a single polypeptide that undergoes autocleavage into two subunits (MUC1-N and MUC1-C) that in turn form a stable heterodimer at the apical membrane of normal epithelial cells. Early research on MUC1 focused on the shed MUC1-N mucin component and led to development of the CA15-3 assay to monitor circulating levels of this subunit as a tumor biomarker. Subsequent work then turned to the MUC1-C transmembrane subunit as the potential link between its overexpression and carcinogenesis. Indeed, the MUC1-C cytoplasmic domain was shown to be sufficient to induce transformation.

With transformation and loss of polarity, MUC1-C associates with EGFR, ErbB2 and other receptor tyrosine kinases at the cell membrane. MUC1-C also localizes to the cytoplasm of transformed cells and is targeted to the nucleus and mitochondria. The MUC1-C cytoplasmic domain directly contributes to the regulation of effectors, such as p53, β-catenin, NF-κB and STATs, that have been linked to transformation. The MUC1-C cytoplasmic domain also functions as a substrate for EGFR, MET, Src family members, c-Abl and GSK3β, supporting a role in diverse signaling pathways.

These findings have provided support for a model in which human tumors overexpress MUC1-C to exploit its role in promoting growth and survival. In addition, the overexpression of MUC1-C in a substantial number of human malignancies has established it as a highly attractive target for the development of targeted agents.

Translational research in the Kufe laboratory has been focused on the development of approaches that target MUC1 with vaccines and with antibodies against the MUC1-C extracellular domain. The demonstration that MUC1-C transforming function is dependent on the formation of oligomers has also provided the experimental framework for designing agents, such as cell-penetrating peptides and small molecules, that block its oligomerization. Based on this work, the first-in-man MUC1-C inhibitor, designated GO-203, has completed Phase I evaluation in patients with refractory solid tumors and a dose has been defined for Phase II trials in hematologic malignancies.

Publications

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