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The DF/HCC High-Throughput Polymorphism Detection Core adds targeted sequencing service


The DF/HCC High Throughput Polymorphism Core / Partners HealthCare | Personalized Medicine TaqMan genotyping core is pleased to announce a new service.

The Core is offering a targeted sequencing service using the Ion Torrent PGM. The capabilities of this service include:

Custom Amplicon Sequencing of DNA and RNA Panels

Ready-to-use Ion AmpliSeq Panels: 

Cancer Hotspot Panel 50 genes, > 2,800 Cosmic Mutations, 207 Amplicons
Comprehensive Cancer Panel 409 genes, 16,000 Amplicons
Inherited Disease Panel 700 disease, 328 genes, 10,000 Amplicons

The system requires only 10ng of input DNA and DNA from FFPE samples can also be used. Additionally, only one or two samples can be run at a time.

The system requires only 10ng of input DNA and DNA from FFPE samples can also be used. Additionally, only one or two samples can be run at a time.

Results can be ready in as little as two days.

There are three different Chip formats:

  • Ion 314 Chip: 1 million wells,  400-550 thousand reads for 200bp sequencing
  • Ion 316 Chip: 6 million wells, 2-3 million reads for 200bp sequencing
  • Ion 318 Chip: 11 million wells, 4-5.5 million reads for 200bp sequencing


Please contact Hardeep Ranu (hranu@hsph.harvard.edu) for more information (including pricing).