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Tissue Microarray Core has re-opened and added services

As of December 3rd, all requests for Tissue Microarray and Nucleic Acid Isolation services should be submitted to the DF/HCC Tissue Microarray Core.  The core remains under the direction of Jon Aster, MD, PhD. Scott Rodig, MD, PhD, will be overseeing all core projects.

When submitting a new work request for these services, login to http://genepath.med.harvard.edu/pathcore, go to the Tissue Microarray Core, and select one of the three service categories:
• TMA Construction Services
• TMA Block Sectioning & Staining Services
• Nucleic Acid Isolation

New Service
The Tissue Microarray Core is now offering Nucleic Acid Isolation from paraffin-embedded tissue cores using the Qiagen BioRobot Workstation. This service enables the high-throughput isolation of genomic DNA and RNA from formalin-fixed paraffin-embedded tissues.  Tissue punches can be taken at the time of construction of tissue microarrays.

NUCLEIC ACID ISOLATION
DNA Isolation From Paraffin
• DNA Isolation [3 x 0.6mm cores/ paraffin block/ well] ($4.00 per well)
• Picogreen Estimation of DNA Yield ($1.00 per well)

RNA Isolation From Paraffin
• RNA Isolation [3 x 0.6mm cores/ paraffin block/ well] ($4.00 per well)
• Picogreen Estimation of DNA Yield ($1.00 per well)

*Samples are run in a 96 well format, with a minimum of 24 wells. Investigators providing their own tissue cores for nucleic acid isolation should stop by the core to pick up a specialty 96-well plate, as standard 96-well plates cannot be used for this service.

Core Information:
Location: Brigham and Women’s Hospital, Thorn Building, Room 603C
Contacts:
Scott Rodig MD, PhD [General questions, specific project applications]
srodig@partners.org
617-525-7825

Chungdak Namgyal [TMA Services]
cnamgyal@partners.org
617-732-7723

Yu Yang [Nucleic Acid Isolation Services]
yu_yang@dfci.harvard.edu
617-525-7434