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Specialized Histopathology Services Core

(CF) RMS cells invade into adjacent muscle (C), intestine (D), liver (E), and kidney (F). Dotted line in D outlines the outer edge of effaced intestine. Arrowheads denote striated muscle tumors. The boxed region in E is a magnified view of a striated cell in the liver. Bars: CF, 50 m.

(CF) RMS cells invade into adjacent muscle (C), intestine (D), liver (E), and kidney (F). Dotted line in D outlines the outer edge of effaced intestine. Arrowheads denote striated muscle tumors. The boxed region in E is a magnified view of a striated cell in the liver. Bars: CF, 50 m.

The Specialized Histopathology Services (SHS) Core provides technical and professional pathology services in a variety of experimental organisms and human tissues. Specific services include: routine processing, embedding, cutting, and staining of tissue sections; and the development, implementation, and interpretation of in situ tests for RNAs and proteins. The SHS core also assists with experimental design, test development, and test interpretation.
    
Zebrafish Model of Rhabdomyosarcoma
Leonard Zon, MD (CHB) and colleagues worked on a transgenic zebrafish model of RAS-induced embryonal rhabdomyosarcoma (ERMS), in which animals develop externally visible disease beginning at ten days of life. Cross-species comparisons identified a conserved gene signature found in both zebrafish and human ERMS, but not the histologically distinct alveolar subtype. Transplantation and limiting dilution assays were used to identify the cancer stem cell in dual fluorescently-labeled transgenic zebrafish RMS. It was established that the cancer stem cell in zebrafish ERMS shares a common gene expression signature with non-transformed satellite cells, suggesting that the satellite cell component may be the target for transformation in embryonal rhabdomyosarcoma. This work was published in Genes and Development.

The core was responsible for the processing and sectioning of the zebrafish, performance of immunohistochemistry, in situ hybridization, and interpretation of pathologic findings and tests results.