DNA Resource Core
The DNA Resource Core provides DNA sequencing services and sequence verified plasmid constructs. Core services include large- and small-scale DNA sequencing, DNA preparation in advance of DNA sequencing, no-charge re-runs of failed sequencing reactions, plasmid storage in an automated, quality-controlled freezer storage system, and storage of plasmid information supported by the Plasmid Information Database.
The DNA Resource Core facilitates a wide variety of sequencing-based approaches. One example is the coreís work with Myron Essex, PhD (HSPH) and his laboratory's projects regarding the prevalence of HIV-1 infection and HIV-1 variants in hotel and bar workers in Tanzania. The core is contributing to this research by sequencing parts of the HIV-1 virus from a cohort of 800 women who work in bars and hotels in Moshi Town Tanzania.
The coreís plasmid repository contributes to member research in two ways. First, the repository eliminates the need for researchers to spend their own time on maintenance and distribution of plasmids. Second, it makes it possible for anyone with access to an on-line terminal to search for and request specific plasmids from a centralized location with minimal delay.
For example, a focus of the laboratory of Matthew Meyerson, MD, PhD (DFCI), is how alterations in the genome cause cancer. Research from this laboratory identified a role for the tumor suppressor menin in chromatin regulation. Events that cause mutations to or loss of expression of menin are associated with an inheritable cancer syndrome, Multiple Endocrine Neoplasia, type I. Meyerson researchers had preliminary data indicating that menin could form a dimer and that mutations in these cancer syndrome patients prevented this dimerization. They used several plasmids from the core repository as critical controls to test whether menin does indeed form a dimer and whether dimerization affects its ability to regulate chromatin. Several other plasmids from the core repository were potential candidate menin interacting proteins that are currently being tested for their contribution to meninís chromatin regulating activity.