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DF/HCC Collaborative RNAi Core offers new services

New laser scanning cytometer (left) with robotics (center) and cell culture incubator (right).

The DF/HCC Collaborative RNAi Core is pleased to announce the availability of three new miRNA-targeted libraries and a new laser scanning cytometer.

New libraries:  Two miRNA “mimic” libraries (Qiagen, Ambion) and one miRNA inhibitor library (Exiqon) for use with human cells are now available through one of our participating groups, the ICCB-Longwood Screening Facility.  More information on these libraries is available here and a full list of RNAi screening libraries offered by the RNAi Core is available here.

New instrument: MDS Analytical Technologies Blueshift IsoCyte.

Instrument features include:

  • 488 and 532 nm excitation and several emission filters
  • Rapid low-magnification imaging of whole wells from 96-, 384- or 1536-well plates
  • Image analysis using automated algorithms
  • Great for GFP or RFP detection in cultured cell lines
  • Light-scatter mode for phase-like imaging
  • Large depth of focus (facilitates imaging of whole animals like C. elegans and zebrafish)
  • Also useful for anisotropy or fluorescence polarization (FP) and fluorescence resonance energy transfer (FRET).

 

The instrument was funded by NIH through an S10 shared instrumentation grant and is located at the ICCB-Longwood Screening Facility at Harvard Medical School.

DF/HCC members are welcome to contact core coordinator S. Mohr for more information, or be in touch with the ICCB-Longwood Screening Facility directly to discuss using the miRNA mimic and inhibitor libraries or the laser scanning cytometer.