DF/HCC Program Affiliation

Prostate Cancer
Angiogenesis, Invasion, and Metastasis

Research Abstract

We are currently focusing on the biological function of angiogenin, an angiogenic ribonuclease that undergoes nuclear translocation in proliferating endothelial cells and in various cancer cells. Angiogenin binds at the promoter region of the ribosomal RNA gene and induces rRNA transcription thereby playing a role in ribosome biogenesis, protein translation and therefore, cell proliferation. Silencing of angiogenin expression in cancer cells resulted in reduced cell growth rate and a reversal of the malignant phenotype. In endothelial cells, inhibition of angiogenin expression decreased cell proliferation induced by other growth stimuli. Thus, angiogenin-stimulated rRNA transcription may play a dual role in cancer progression.

Publications

• Chen Y, Zhou X, Chen X, Hu G.Arginine methylation of hnRNP K enhances p53 transcriptional activity.FEBS Lett 2008 May 7.
  18472002
• Hao DX, Gong FL, Wei W, Hu GH, Ma GH, Su ZG.Porogen effects in synthesis of uniform micrometer-sized poly(divinylbenzene) microspheres with high surface areas.J Colloid Interface Sci 2008 Apr 30.
  18455174
• Wang H, Leav I, Ibaragi S, Wegner M, Hu GF, Lu ML, Balk SP, Yuan X.SOX9 is expressed in human fetal prostate epithelium and enhances prostate cancer invasion.Cancer Res 2008 Mar 15;68(6):1625-30.
  18339840
• Wu D, Yu W, Kishikawa H, Folkerth RD, Iafrate AJ, Shen Y, Xin W, Sims K, Hu GF.Angiogenin loss-of-function mutations in amyotrophic lateral sclerosis.Ann Neurol 2007 Dec;62(6):609-17.
  17886298
• Tsuji T, Katsurano M, Ibaragi S, Shima K, Sasaki A, Hu GF.Ornithine decarboxylase antizyme upregulates DNA-dependent protein kinase and enhances the nonhomologous end-joining repair of DNA double-strand breaks in human oral cancer cells.Biochemistry 2007
  17630775
• Schwartz B, Shoseyov O, Melnikova VO, McCarty M, Leslie M, Roiz L, Smirnoff P, Hu GF, Lev D, Bar-Eli M.ACTIBIND, a T2 RNase, competes with angiogenin and inhibits human melanoma growth, angiogenesis, and metastasis.Cancer Res 2007 Jun 1;67(11):5258-66.
  17545605
• Tsuji T, Sun Y, Kishimoto K, Olson KA, Liu S, Hirukawa S, Hu GF.Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation.Cancer Res 2005 Feb 15;65(4):1352-60.
  15735021
• Kishimoto K, Liu S, Tsuji T, Olson KA, Hu GF.Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis.Oncogene 2005 Jan 13;24(3):445-56.
  15558023
• Hu MG, Hu GF, Kim Y, Tsuji T, McBride J, Hinds P, Wong DT.Role of p12(CDK2-AP1) in transforming growth factor-beta1-mediated growth suppression.Cancer Res 2004 Jan 15;64(2):490-9.
  14744761
• Xu ZP, Tsuji T, Riordan JF, Hu GF.Identification and characterization of an angiogenin-binding DNA sequence that stimulates luciferase reporter gene expression.Biochemistry 2003 Jan 14;42(1):121-8.
  12515546
• Xu ZP, Tsuji T, Riordan JF, Hu GF.The nuclear function of angiogenin in endothelial cells is related to rRNA production.Biochem Biophys Res Commun 2002 Jun 7;294(2):287-92.
  12051708
• Hu GF.Neomycin inhibits the angiogenic activity of fibroblast and epidermal growth factors.Biochem Biophys Res Commun 2001 Oct 5;287(4):870-4.
  11573945
• Liu S, Yu D, Xu ZP, Riordan JF, Hu GF.Angiogenin activates Erk1/2 in human umbilical vein endothelial cells.Biochem Biophys Res Commun 2001 Sep 14;287(1):305-10.
  11549292
• Tsuji T, Usui S, Aida T, Tachikawa T, Hu GF, Sasaki A, Matsumura T, Todd R, Wong DT.Induction of epithelial differentiation and DNA demethylation in hamster malignant oral keratinocyte by ornithine decarboxylase antizyme.Oncogene 2001 Jan 4;20(1):24-33.
  11244502
• Hu GF.Neomycin inhibits angiogenin-induced angiogenesis.Proc Natl Acad Sci U S A 1998 Aug 18;95(17):9791-5.
  9707554
• Hu GF.Limited proteolysis of angiogenin by elastase is regulated by plasminogen.J Protein Chem 1997 Oct;16(7):669-79.
  9330225
• Hu GF, Riordan JF, Vallee BL.A putative angiogenin receptor in angiogenin-responsive human endothelial cells.Proc Natl Acad Sci U S A 1997 Mar 18;94(6):2204-9.
  9122172
• Schwalb JM, Gu MF, Stuermer C, Bastmeyer M, Hu GF, Boulis N, Irwin N, Benowitz LI.Optic nerve glia secrete a low-molecular-weight factor that stimulates retinal ganglion cells to regenerate axons in goldfish.Neuroscience 1996 Jun;72(4):901-10.
  8735218
• Hu GF.Fluorophore-assisted carbohydrate electrophoresis technology and applications.J Chromatogr A 1995 Jun 23;705(1):89-103.
  7620574
• Hu GF, Riordan JF.Angiogenin enhances actin acceleration of plasminogen activation.Biochem Biophys Res Commun 1993 Dec 15;197(2):682-7.
  8267604
• Hu GF, Strydom DJ, Fett JW, Riordan JF, Vallee BL.Actin is a binding protein for angiogenin.Proc Natl Acad Sci U S A 1993 Feb 15;90(4):1217-21.
  7679494
• Hu GF, Chang SI, Riordan JF, Vallee BL.An angiogenin-binding protein from endothelial cells.Proc Natl Acad Sci U S A 1991 Mar 15;88(6):2227-31.
  2006162