DF/HCC Program Affiliation

Angiogenesis, Invasion, and Metastasis

Research Abstract

This laboratory studies tissue and organ morphogenesis. Work is currently directed at three project areas.

In the first project we study skeletal morphogenesis and growth. We are interested in genes that control differentiation of mesenchymal cells to chondrocytes and osteoblasts, the control of spatial patterns of mesenchymal condensations during skeletal development and tooth formation, the molecular mechanisms controlling the formation of ossification centers, and the regulation of proliferation and differentiation of chondrocytes in growth plates. In addition to using transgenic mice in studies of specific genes, we make extensive use of genetic approaches in mice and humans. This includes mapping of inherited disorders, gene identification and mutation detection.

In the second project we investigate the molecular basis for vascular morphogenesis, using a combination of human genetics and studies of cells in culture. In addition, we use gene targeting to generate mice with inactivated alleles for collagens that are expressed in vascular walls.

In the third project we are studying genetic risk factors for osteoarthritis in humans. One approach involves identification of mutations responsible for early-onset osteoarthritis as part of inherited osteochondrodysplasias; in other studies we are screening a population of patients with osteoarthritis for mutations in candidate genes.

Publications

• Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K.Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.Nat Cell
  18425119
• Thegerstrom J, Romanus V, Friman V, Brudin L, Haemig PD, Olsen B.Mycobacterium avium lymphadenopathy among children, Sweden.Emerg Infect Dis 2008 Apr;14(4):661-3.
  18394289
• Haemig PD, Hernandez J, Waldenstrom J, Bonnedahl J, Olsen B.Barn Swallows (Hirundo rustica) Test Negative for Salmonella.Vector Borne Zoonotic Dis 2008 Feb 11.
  18266565
• Glotzer DJ, Zelzer E, Olsen BR.Impaired skin and hair follicle development in Runx2 deficient mice.Dev Biol 2008 Mar 15;315(2):459-73.
  18262513
• Sjolund M, Bonnedahl J, Hernandez J, Bengtsson S, Cederbrant G, Pinhassi J, Kahlmeter G, Olsen B.Dissemination of multidrug-resistant bacteria into the Arctic.Emerg Infect Dis 2008 Jan;14(1):70-2.
  18258081
• Greene AK, Kim S, Rogers GF, Fishman SJ, Olsen BR, Mulliken JB.Risk of vascular anomalies with Down syndrome.Pediatrics 2008 Jan;121(1):e135-40.
  18166531
• Waldenstrom J, Lundkvist A, Falk KI, Garpmo U, Bergstrom S, Lindegren G, Sjostedt A, Mejlon H, Fransson T, Haemig PD, Olsen B.Migrating birds and tickborne encephalitis virus.Emerg Infect Dis 2007 Aug;13(8):1215-8.
  17953095
• Fick J, Lindberg RH, Tysklind M, Haemig PD, Waldenstrom J, Wallensten A, Olsen B.Antiviral oseltamivir is not removed or degraded in normal sewage water treatment: implications for development of resistance by influenza A virus.PLoS ONE 2007;2(10):e986.
  17912363
• Axelsson-Olsson D, Ellstrom P, Waldenstrom J, Haemig PD, Brudin L, Olsen B.Acanthamoeba-Campylobacter coculture as a novel method for enrichment of Campylobacter species.Appl Environ Microbiol 2007 Nov;73(21):6864-9.
  17873069
• Wallensten A, Waldenstrom J, Olsen B.[Avian influenza, the hen, the man and the presumed death].Lakartidningen 2007 Aug 8-21;104(32-33):2240-3.
  17822202
• Larsson C, Comstedt P, Olsen B, Bergstrom S.First record of Lyme disease Borrelia in the Arctic.Vector Borne Zoonotic Dis 2007 Fall;7(3):453-6.
  17767412
• Kolpakova-Hart E, Jinnin M, Hou B, Fukai N, Olsen BR.Kinesin-2 controls development and patterning of the vertebrate skeleton by Hedgehog- and Gli3-dependent mechanisms.Dev Biol 2007 Sep 15;309(2):273-84.
  17698054
• Xu L, Peng H, Glasson S, Lee PL, Hu K, Ijiri K, Olsen BR, Goldring MB, Li Y.Increased expression of the collagen receptor discoidin domain receptor 2 in articular cartilage as a key event in the pathogenesis of osteoarthritis.Arthritis Rheum 2007 Aug;56(8
  17665456
• Li Y, Xu L, Olsen BR.Lessons from genetic forms of osteoarthritis for the pathogenesis of the disease.Osteoarthritis Cartilage 2007 Oct;15(10):1101-5.
  17572112
• Marneros AG, She H, Zambarakji H, Hashizume H, Connolly EJ, Kim I, Gragoudas ES, Miller JW, Olsen BR.Endogenous endostatin inhibits choroidal neovascularization.FASEB J 2007 May 25.
  17526870
• Karlsson M, Wallensten A, Lundkvist A, Olsen B, Brytting M.A real-time PCR assay for the monitoring of influenza A virus in wild birds.J Virol Methods 2007 Sep;144(1-2):27-31.
  17482684
• Legare F, Pfeffer C, Olsen BR.The role of backscattering in SHG tissue imaging.Biophys J 2007 Aug 15;93(4):1312-20.
  17449666
• Hou B, Fukai N, Olsen BR.Mechanical force-induced midpalatal suture remodeling in mice.Bone 2007 Jun;40(6):1483-93.
  17398175
• Ueki Y, Lin CY, Senoo M, Ebihara T, Agata N, Onji M, Saheki Y, Kawai T, Mukherjee PM, Reichenberger E, Olsen BR.Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 "cherubism" mice.Cell 2007 Jan 12;128(
  17218256
• Lam NP, Li Y, Waldman AB, Brussiau J, Lee PL, Olsen BR, Xu L.Age-dependent increase of discoidin domain receptor 2 and matrix metalloproteinase 13 expression in temporomandibular joint cartilage of type IX and type XI collagen-deficient mice.Arch Oral Bio
  17125729
• Marneros AG, Fan J, Yokoyama Y, Gerber HP, Ferrara N, Crouch RK, Olsen BR.Vascular endothelial growth factor expression in the retinal pigment epithelium is essential for choriocapillaris development and visual function.Am J Pathol 2005 Nov;167(5):1451-9.
  16251428
• Xu L, Peng H, Wu D, Hu K, Goldring MB, Olsen BR, Li Y.Activation of the discoidin domain receptor 2 induces expression of matrix metalloproteinase 13 associated with osteoarthritis in mice.J Biol Chem 2005 Jan 7;280(1):548-55.
  15509586