DF/HCC Program Affiliation

Cancer Genetics
Cancer Cell Biology

Research Abstract

Our laboratory uses Drosophila as a model system to characterize evolutionary conserved signaling transduction pathways. Our current studies focus on the analysis of Wnt, EGF Receptor, and JAK/STAT signaling transduction pathways. In addition, other projects in the lab focus on the characterization of signaling pathways that regulate the establishment of polarity and cytoskeletal organization.

Publications

• Lu J, Ruhf ML, Perrimon N, Leder P.A genome-wide RNA interference screen identifies putative chromatin regulators essential for E2F repression.Proc Natl Acad Sci U S A 2007 May 29;104(22):9381-6.
  17517653
• Mathey-Prevot B, Perrimon N.Do-it-yourself RNAi made easy?.Nat Methods 2007 Apr;4(4):308-9.
  17396126
• Mathey-Prevot B, Perrimon N.Drosophila genome-wide RNAi screens: are they delivering the promise?.Cold Spring Harb Symp Quant Biol 2006;71:141-8.
  17381290
• Friedman A, Perrimon N.Genetic screening for signal transduction in the era of network biology.Cell 2007 Jan 26;128(2):225-31.
  17254958
• Perrimon N, Mathey-Prevot B.Applications of high-throughput RNA interference screens to problems in cell and developmental biology.Genetics 2007 Jan;175(1):7-16.
  17244760
• Perrimon N, Friedman A, Mathey-Prevot B, Eggert US.Drug-target identification in Drosophila cells: combining high-throughout RNAi and small-molecule screens.Drug Discov Today 2007 Jan;12(1-2):28-33.
  17198970
• Friedman A, Perrimon N.A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling.Nature 2006 Nov 9;444(7116):230-4.
  17086199
• Bach EA, Ekas LA, Ayala-Camargo A, Flaherty MS, Lee H, Perrimon N, Baeg GH.GFP reporters detect the activation of the Drosophila JAK/STAT pathway in vivo.Gene Expr Patterns 2007 Jan;7(3):323-31.
  17008134
• Echeverri CJ, Beachy PA, Baum B, Boutros M, Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG, Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L, Ma Y, Mathey-Prévôt B, Root DE, Sabatini DM, Taipale J, Perrimon N, Bernards R.Minimizing th
  16990807
• Kulkarni MM, Booker M, Silver SJ, Friedman A, Hong P, Perrimon N, Mathey-Prevot B.Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays.Nat Methods 2006 Oct;3(10):833-8.
  16964256
• Gibson MC, Patel AB, Nagpal R, Perrimon N.The emergence of geometric order in proliferating metazoan epithelia.Nature 2006 Aug 31;442(7106):1038-41.
  16900102
• Friedman A, Perrimon N.High-throughput approaches to dissecting MAPK signaling pathways.Methods 2006 Jul 14.
  16844385
• Chen LY, Wang JC, Hyvert Y, Lin HP, Perrimon N, Imler JL, Hsu JC.Weckle is a zinc finger adaptor of the toll pathway in dorsoventral patterning of the Drosophila embryo.Curr Biol 2006 Jun 20;16(12):1183-93.
  16782008
• Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N, Malhotra V.Functional genomics reveals genes involved in protein secretion and Golgi organization.Nature 2006 Feb 2;439(7076):604-7.
  16452979
• Flockhart I, Booker M, Kiger A, Boutros M, Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N, Mathey-Prevot B.FlyRNAi: the Drosophila RNAi screening center database.Nucleic Acids Res 2006 Jan 1;34(Database issue):D489-94.
  16381918
• Micchelli CA, Perrimon N.Evidence that stem cells reside in the adult Drosophila midgut epithelium.Nature 2006 Jan 26;439(7075):475-9.
  16340959
• Nybakken K, Vokes SA, Lin TY, McMahon AP, Perrimon N.A genome-wide RNA interference screen in Drosophila melanogaster cells for new components of the Hh signaling pathway.Nat Genet 2005 Dec;37(12):1323-32.
  16311596
• Karsten P, Plischke I, Perrimon N, Zeidler MP.Mutational analysis reveals separable DNA binding and trans-activation of Drosophila STAT92E.Cell Signal 2006 Jun;18(6):819-29.
  16129580
• Ghiglione C, Carraway KL, Amundadottir LT, Boswell RE, Perrimon N, Duffy JB.The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis.Cell 1999 Mar 19;96(6):847-56.
  10102272
• Czech B, Malone CD, Zhou R, Stark A, Schlingeheyde C, Dus M, Perrimon N, Kellis M, Wohlschlegel JA, Sachidanandam R, Hannon GJ, Brennecke J.An endogenous small interfering RNA pathway in Drosophila.Nature 2008 May 7.
  18463631
• Bai J, Binari R, Ni JQ, Vijayakanthan M, Li HS, Perrimon N.RNA interference screening in Drosophila primary cells for genes involved in muscle assembly and maintenance.Development 2008 Apr;135(8):1439-49.
  18359903
• Markstein M, Pitsouli C, Villalta C, Celniker SE, Perrimon N.Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes.Nat Genet 2008 Apr;40(4):476-83.
  18311141
• Philips JA, Porto MC, Wang H, Rubin EJ, Perrimon N.ESCRT factors restrict mycobacterial growth.Proc Natl Acad Sci U S A 2008 Feb 26;105(8):3070-5.
  18287038
• Wang J, Zhou X, Bradley PL, Chang SF, Perrimon N, Wong ST.Cellular phenotype recognition for high-content RNA interference genome-wide screening.J Biomol Screen 2008 Jan;13(1):29-39.
  18227224
• Ni JQ, Markstein M, Binari R, Pfeiffer B, Liu LP, Villalta C, Booker M, Perkins L, Perrimon N.Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.Nat Methods 2008 Jan;5(1):49-51.
  18084299
• Bai J, Hartwig JH, Perrimon N.SALS, a WH2-domain-containing protein, promotes sarcomeric actin filament elongation from pointed ends during Drosophila muscle growth.Dev Cell 2007 Dec;13(6):828-42.
  18061565
• Leopold P, Perrimon N.Drosophila and the genetics of the internal milieu.Nature 2007 Nov 8;450(7167):186-8.
  17994083
• Silver SJ, Hagen JW, Okamura K, Perrimon N, Lai EC.Functional screening identifies miR-315 as a potent activator of Wingless signaling.Proc Natl Acad Sci U S A 2007 Nov 13;104(46):18151-6.
  17989227
• Bakal C, Aach J, Church G, Perrimon N.Quantitative morphological signatures define local signaling networks regulating cell morphology.Science 2007 Jun 22;316(5832):1753-6.
  17588932
• Baeg GH, Zhou R, Perrimon N.Genome-wide RNAi analysis of JAK/STAT signaling components in Drosophila.Genes Dev 2005 Aug 15;19(16):1861-70.
  16055650
• DasGupta R, Kaykas A, Moon RT, Perrimon N.Functional genomic analysis of the Wnt-wingless signaling pathway.Science 2005 May 6;308(5723):826-33.
  15817814
• Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, Field CM.Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets.PLoS Biol 2004 Dec;2(12):e379.
  15547975
• Agaisse H, Petersen UM, Boutros M, Mathey-Prevot B, Perrimon N.Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to septic injury.Dev Cell 2003 Sep;5(3):441-50.
  12967563
• Wills Z, Emerson M, Rusch J, Bikoff J, Baum B, Perrimon N, Van Vactor D.A Drosophila homolog of cyclase-associated proteins collaborates with the Abl tyrosine kinase to control midline axon pathfinding.Neuron 2002 Nov 14;36(4):611-22.
  12441051
• Lin X, Buff EM, Perrimon N, Michelson AM.Heparan sulfate proteoglycans are essential for FGF receptor signaling during Drosophila embryonic development.Development 1999 Sep;126(17):3715-23.
  10433902
• Ghiglione C, Perrimon N, Perkins LA.Quantitative variations in the level of MAPK activity control patterning of the embryonic termini in Drosophila.Dev Biol 1999 Jan 1;205(1):181-93.
  9882506
• Hou XS, Perrimon N.The JAK-STAT pathway in Drosophila.Trends Genet 1997 Mar;13(3):105-10.
  9066269
• Yu Y, Li W, Su K, Yussa M, Han W, Perrimon N, Pick L.The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz.Nature 1997 Feb 6;385(6616):552-5.
  9020364
• Kadowaki T, Wilder E, Klingensmith J, Zachary K, Perrimon N.The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing.Genes Dev 1996 Dec 15;10(24):3116-28.
  8985181
• Perrimon N, Lanjuin A, Arnold C, Noll E.Zygotic lethal mutations with maternal effect phenotypes in Drosophila melanogaster. II. Loci on the second and third chromosomes identified by P-element-induced mutations.Genetics 1996 Dec;144(4):1681-92.
  8978055
• Perkins LA, Johnson MR, Melnick MB, Perrimon N.The nonreceptor protein tyrosine phosphatase corkscrew functions in multiple receptor tyrosine kinase pathways in Drosophila.Dev Biol 1996 Nov 25;180(1):63-81.
  8948575
• Rulifson EJ, Micchelli CA, Axelrod JD, Perrimon N, Blair SS.wingless refines its own expression domain on the Drosophila wing margin.Nature 1996 Nov 7;384(6604):72-4.
  8900280
• Duffy JB, Perrimon N.Recent advances in understanding signal transduction pathways in worms and flies.Curr Opin Cell Biol 1996 Apr;8(2):231-8.
  8791421
• Cleghon V, Gayko U, Copeland TD, Perkins LA, Perrimon N, Morrison DK.Drosophila terminal structure development is regulated by the compensatory activities of positive and negative phosphotyrosine signaling sites on the Torso RTK.Genes Dev 1996 Mar 1;10(5)
  8598287
• Perrimon N, Lu X, Hou XS, Hsu JC, Melnick MB, Chou TB, Perkins LA.Dissection of the Torso signal transduction pathway in Drosophila.Mol Reprod Dev 1995 Dec;42(4):515-22.
  8607984
• Perkins LA, Larsen I, Perrimon N.corkscrew encodes a putative protein tyrosine phosphatase that functions to transduce the terminal signal from the receptor tyrosine kinase torso.Cell 1992 Jul 24;70(2):225-36.
  1638629