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Vadim Gladyshev, PhD


  • Turanov AA, Shchedrina VA, Everley RA, Lobanov AV, Yim SH, Marino SM, Gygi SP, Hatfield DL, Gladyshev VN.Selenoprotein S is Involved in Maintenance and Transport of Multiprotein Complexes.Biochem J. 2014 Jun 4.
  • Avanesov AS, Ma S, Pierce KA, Yim SH, Lee BC, Clish CB, Gladyshev VN.Age- and diet-associated metabolome remodeling characterizes the aging process driven by damage accumulation.Elife. 2014;3:e02077.
  • Han SJ, Lee BC, Yim SH, Gladyshev VN, Lee SR.Characterization of Mammalian selenoprotein o: a redox-active mitochondrial protein.PLoS ONE. 2014;9(4):e95518.
  • Barroso M, Florindo C, Kalwa H, Silva Z, Turanov AA, Carlson BA, Tavares de Almeida I, Blom HJ, Gladyshev VN, Hatfield DL, Michel T, Castro R, Loscalzo J, Handy DE.Inhibition of Cellular Methyltransferases Promotes Endothelial Cell Activation by Suppressing Glutathione Peroxidase-1 Expression.J Biol Chem. 2014 Apr 9.
  • Lee BC, Kaya A, Ma S, Kim G, Gerashchenko MV, Yim SH, Hu Z, Harshman LG, Gladyshev VN.Methionine restriction extends lifespan of Drosophila melanogaster under conditions of low amino-acid status.Nat Commun. 2014;5:3592.
  • Hatfield DL, Tsuji PA, Carlson BA, Gladyshev VN.Selenium and selenocysteine: roles in cancer, health, and development.Trends Biochem Sci. 2014 Mar;39(3):112-20.
  • Malinouski M, Hasan NM, Zhang Y, Seravalli J, Lin J, Avanesov A, Lutsenko S, Gladyshev VN.Genome-wide RNAi ionomics screen reveals new genes and regulation of human trace element metabolism.Nat Commun. 2014;5:3301.
  • Labunskyy VM, Gerashchenko MV, Delaney JR, Kaya A, Kennedy BK, Kaeberlein M, Gladyshev VN.Lifespan extension conferred by endoplasmic reticulum secretory pathway deficiency requires induction of the unfolded protein response.PLoS Genet. 2014 Jan;10(1):e1004019.
  • Romagné F, Santesmasses D, White L, Sarangi GK, Mariotti M, Hübler R, Weihmann A, Parra G, Gladyshev VN, Guigó R, Castellano S.SelenoDB 2.0: annotation of selenoprotein genes in animals and their genetic diversity in humans.Nucleic Acids Res. 2014 Jan;42(Database issue):D437-43.
  • Yoo MH, Carlson BA, Gladyshev VN, Hatfield DL.Abrogated thioredoxin system causes increased sensitivity to TNF-α-induced apoptosis via enrichment of p-ERK 1/2 in the nucleus.PLoS ONE. 2013;8(9):e71427.
  • Naranjo-Suarez S, Carlson BA, Tobe R, Yoo MH, Tsuji PA, Gladyshev VN, Hatfield DL.Regulation of HIF-1α activity by overexpression of thioredoxin is independent of thioredoxin reductase status.Mol Cells. 2013 Aug;36(2):151-7.
  • Turanov AA, Lobanov AV, Hatfield DL, Gladyshev VN.UGA codon position-dependent incorporation of selenocysteine into mammalian selenoproteins.Nucleic Acids Res. 2013 Aug;41(14):6952-9.
  • Kasaikina MV, Turanov AA, Avanesov A, Schweizer U, Seeher S, Bronson RT, Novoselov SN, Carlson BA, Hatfield DL, Gladyshev VN.Contrasting roles of dietary selenium and selenoproteins in chemically induced hepatocarcinogenesis.Carcinogenesis. 2013 Feb 6.
  • Gerashchenko MV, Lobanov AV, Gladyshev VN.Genome-wide ribosome profiling reveals complex translational regulation in response to oxidative stress.Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17394-9.
  • Gladyshev VN.On the cause of aging and control of lifespan: Heterogeneity leads to inevitable damage accumulation, causing aging; Control of damage composition and rate of accumulation define lifespan.Bioessays. 2012 Nov;34(11):925-9.
  • Labunskyy VM, Gladyshev VN.Role of Reactive Oxygen Species-Mediated Signaling in Aging.Antioxid Redox Signal. 2012 Sep 20.
  • Carlson BA, Yoo MH, Tobe R, Mueller C, Naranjo-Suarez S, Hoffmann VJ, Gladyshev VN, Hatfield DL.Thioredoxin reductase 1 protects against chemically induced hepatocarcinogenesis via control of cellular redox homeostasis.Carcinogenesis. 2012 Sep;33(9):1806-13.
  • Tobe R, Yoo MH, Fradejas N, Carlson BA, Calvo S, Gladyshev VN, Hatfield DL.Thioredoxin reductase 1 deficiency enhances selenite toxicity in cancer cells via a thioredoxin-independent mechanism.Biochem J. 2012 Aug 1;445(3):423-30.
  • Wu C, Parrott AM, Fu C, Liu T, Marino SM, Gladyshev VN, Jain MR, Baykal AT, Li Q, Oka S, Sadoshima J, Beuve A, Simmons WJ, Li H.Thioredoxin 1-Mediated Post-Translational Modifications: Reduction, Transnitrosylation, Denitrosylation and Related Proteomics Methodologies.Antioxid Redox Signal. 2011 Apr 1.
  • Xu XM, Turanov AA, Carlson BA, Yoo MH, Everley RA, Nandakumar R, Sorokina I, Gygi SP, Gladyshev VN, Hatfield DL.Targeted insertion of cysteine by decoding UGA codons with mammalian selenocysteine machinery.Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21430-4.
  • Lee BC, Lobanov AV, Marino SM, Kaya A, Seravalli J, Hatfield DL, Gladyshev VN.A four selenocysteine, two SECIS element methionine sulfoxide reductase from Metridium senile reveals a non-catalytic function of selenocysteines.J Biol Chem. 2011 Mar 10.
  • Kasaikina MV, Lobanov AV, Malinouski MY, Lee BC, Seravalli J, Fomenko DE, Turanov AA, Finney L, Vogt S, Park TJ, Miller RA, Hatfield DL, Gladyshev VN.Reduced utilization of selenium by naked mole rats due to a specific defect in GPx1 expression.J Biol Chem. 2011 Mar 3.
  • Kim MJ, Lee BC, Jeong J, Lee KJ, Hwang KY, Gladyshev VN, Kim HY.Tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase.Mol Microbiol. 2011 Mar;79(5):1194-203.
  • Fomenko DE, Koc A, Agisheva N, Jacobsen M, Kaya A, Malinouski M, Rutherford JC, Siu KL, Jin DY, Winge DR, Gladyshev VN.Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide.Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2729-34.
  • Malinouski M, Zhou Y, Belousov VV, Hatfield DL, Gladyshev VN.Hydrogen peroxide probes directed to different cellular compartments.PLoS ONE. 2011;6(1):e14564.
  • Suzuki Y, St Onge RP, Mani R, King OD, Heilbut A, Labunskyy VM, Chen W, Pham L, Zhang LV, Tong AH, Nislow C, Giaever G, Gladyshev VN, Vidal M, Schow P, Lehár J, Roth FP.Knocking out multigene redundancies via cycles of sexual assortment and fluorescence selection.Nat Methods. 2011 Feb;8(2):159-64.
  • Marino SM, Gladyshev VN.Proteomics: mapping reactive cysteines.Nat Chem Biol. 2011 Feb;7(2):72-3.
  • Lee BC, Gladyshev VN.The biological significance of methionine sulfoxide stereochemistry.Free Radic Biol Med. 2011 Jan 15;50(2):221-7.
  • Hacioglu E, Esmer I, Fomenko DE, Gladyshev VN, Koc A.The roles of thiol oxidoreductases in yeast replicative aging.Mech Ageing Dev.;131(11-12):692-9.
  • Kaya A, Koc A, Lee BC, Fomenko DE, Rederstorff M, Krol A, Lescure A, Gladyshev VN.Compartmentalization and Regulation of Mitochondrial Function by Methionine Sulfoxide Reductases in Yeast.Biochemistry. 2010 Oct 5;49(39):8618-25.
  • Marino SM, Gladyshev VN.Cysteine Function Governs Its Conservation and Degeneration and Restricts Its Utilization on Protein Surfaces.J Mol Biol. 2010 Dec 17;404(5):902-16.
  • Aachmann FL, Sal LS, Kim HY, Marino SM, Gladyshev VN, Dikiy A.Insights into function, catalytic mechanism, and fold evolution of selenoprotein methionine sulfoxide reductase B1 through structural analysis.J Biol Chem. 2010 Oct 22;285(43):33315-23.
  • Marino SM, Gladyshev V.Redox Biology: Computational Approaches to the Investigation of Functional Cysteine Residues.Antioxid Redox Signal. 2010 Sep 2.
  • Sengupta A, Lichti UF, Carlson BA, Ryscavage AO, Gladyshev VN, Yuspa SH, Hatfield DL.Selenoproteins are essential for proper keratinocyte function and skin development.PLoS ONE. 2010;5(8):e12249.
  • Marino SM, Li Y, Fomenko DE, Agisheva N, Cerny RL, Gladyshev VN.Characterization of surface-exposed reactive cysteine residues in Saccharomyces cerevisiae.Biochemistry. 2010 Sep 7;49(35):7709-21.
  • Turanov AA, Kehr S, Marino SM, Yoo MH, Carlson BA, Hatfield DL, Gladyshev VN.Mammalian thioredoxin reductase 1: roles in redox homoeostasis and characterization of cellular targets.Biochem J. 2010 Sep 1;430(2):285-93.
  • Liang X, Fomenko DE, Hua D, Kaya A, Gladyshev VN.Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.PLoS ONE. 2010;5(7):e11497.
  • Carlson BA, Yoo MH, Shrimali RK, Irons R, Gladyshev VN, Hatfield DL, Park JM.Role of selenium-containing proteins in T-cell and macrophage function.Proc Nutr Soc. 2010 Aug;69(3):300-10.
  • Lobanov AV, Turanov AA, Hatfield DL, Gladyshev VN.Dual functions of codons in the genetic code.Crit Rev Biochem Mol Biol. 2010 Aug;45(4):257-65. Review.
  • Yoo MH, Carlson BA, Tsuji P, Irons R, Gladyshev VN, Hatfield DL.Alteration of thioredoxin reductase 1 levels in elucidating cancer etiology.Methods Enzymol. 2010;474:255-75.
  • Turanov AA, Hatfield DL, Gladyshev VN.Characterization of protein targets of mammalian thioredoxin reductases.Methods Enzymol. 2010;474:245-54.
  • Irons R, Tsuji PA, Carlson BA, Ouyang P, Yoo MH, Xu XM, Hatfield DL, Gladyshev VN, Davis CD.Deficiency in the 15-kDa selenoprotein inhibits tumorigenicity and metastasis of colon cancer cells.Cancer Prev Res (Phila Pa). 2010 May;3(5):630-9.
  • Yoo MH, Gu X, Xu XM, Kim JY, Carlson BA, Patterson AD, Cai H, Gladyshev VN, Hatfield DL.Delineating the role of glutathione peroxidase 4 in protecting cells against lipid hydroperoxide damage and in Alzheimer's disease.Antioxid Redox Signal. 2010 Apr 1;12(7):819-27.
  • Novoselov SV, Kim HY, Hua D, Lee BC, Astle CM, Harrison DE, Friguet B, Moustafa ME, Carlson BA, Hatfield DL, Gladyshev VN.Regulation of selenoproteins and methionine sulfoxide reductases A and B1 by age, calorie restriction, and dietary selenium in mice.Antioxid Redox Signal. 2010 Apr 1;12(7):829-38.
  • Shchedrina VA, Zhang Y, Labunskyy VM, Hatfield DL, Gladyshev VN.Structure-function relations, physiological roles, and evolution of mammalian ER-resident selenoproteins.Antioxid Redox Signal. 2010 Apr 1;12(7):839-49. Review.
  • Zhang Y, Gladyshev VN.dbTEU: a protein database of trace element utilization.Bioinformatics. 2010 Mar 1;26(5):700-2.
  • Gerashchenko MV, Su D, Gladyshev VN.CUG start codon generates thioredoxin/glutathione reductase isoforms in mouse testes.J Biol Chem. 2010 Feb 12;285(7):4595-602.
  • Marino SM, Gladyshev VN.Structural analysis of cysteine S-nitrosylation: a modified acid-based motif and the emerging role of trans-nitrosylation.J Mol Biol. 2010 Jan 29;395(4):844-59.
  • Zhang Y, Gladyshev VN.General trends in trace element utilization revealed by comparative genomic analyses of Co, Cu, Mo, Ni, and Se.J Biol Chem. 2010 Jan 29;285(5):3393-405.
  • Bekaert M, Firth AE, Zhang Y, Gladyshev VN, Atkins JF, Baranov PV.Recode-2: new design, new search tools, and many more genes.Nucleic Acids Res. 2010 Jan;38(Database issue):D69-74.
  • Sengupta A, Carlson BA, Labunskyy VM, Gladyshev VN, Hatfield DL.Selenoprotein T deficiency alters cell adhesion and elevates selenoprotein W expression in murine fibroblast cells.Biochem. Cell Biol. 2009 Dec;87(6):953-61.
  • Carlson BA, Yoo MH, Sano Y, Sengupta A, Kim JY, Irons R, Gladyshev VN, Hatfield DL, Park JM.Selenoproteins regulate macrophage invasiveness and extracellular matrix-related gene expression.BMC Immunol. 2009;10:57.
  • Hatfield DL, Yoo MH, Carlson BA, Gladyshev VN.Selenoproteins that function in cancer prevention and promotion.Biochim Biophys Acta. 2009 Nov;1790(11):1541-5. Review.
  • Lee BC, Dikiy A, Kim HY, Gladyshev VN.Functions and evolution of selenoprotein methionine sulfoxide reductases.Biochim Biophys Acta. 2009 Nov;1790(11):1471-7. Review.
  • Lobanov AV, Hatfield DL, Gladyshev VN.Eukaryotic selenoproteins and selenoproteomes.Biochim Biophys Acta. 2009 Nov;1790(11):1424-8. Review.
  • Jin BY, Sartoretto JL, Gladyshev VN, Michel T.Endothelial nitric oxide synthase negatively regulates hydrogen peroxide-stimulated AMP-activated protein kinase in endothelial cells.Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17343-8.
  • Zhang Y, Gladyshev VN.Comparative genomics of trace elements: emerging dynamic view of trace element utilization and function.Chem. Rev. . 2009 Oct;109(10):4828-61. Review.
  • Carlson BA, Yoo MH, Tsuji PA, Gladyshev VN, Hatfield DL.Mouse models targeting selenocysteine tRNA expression for elucidating the role of selenoproteins in health and development.Molecules. 2009;14(9):3509-27. Review.
  • Labunskyy VM, Yoo MH, Hatfield DL, Gladyshev VN.Sep15, a thioredoxin-like selenoprotein, is involved in the unfolded protein response and differentially regulated by adaptive and acute ER stresses.Biochemistry. 2009 Sep 8;48(35):8458-65.
  • Xu XM, Yoo MH, Carlson BA, Gladyshev VN, Hatfield DL.Simultaneous knockdown of the expression of two genes using multiple shRNAs and subsequent knock-in of their expression.Nat Protoc. 2009;4(9):1338-48.
  • Kaya A, Karakaya HC, Fomenko DE, Gladyshev VN, Koc A.Identification of a novel system for boron transport: Atr1 is a main boron exporter in yeast.Mol Cell Biol. 2009 Jul;29(13):3665-74.
  • Turanov A.A., Lobanov A.V., Fomenko D.E., Morrison H.G., Sogin M.L., Klobutcher L.A., Hatfield D.L., Gladyshev V.N. .Genetic code supports targeted insertion of two amino acids by one codon.Science.;323:259-261.
  • Fomenko, D. E., Xing, W., Adair, B. M., Thomas, D. J., and Gladyshev, V. N. .High-throughput identification of catalytic redox-active cysteine residues.Science.;135:387-389.
  • 44) Kryukov, G. V., Castellano, S., Novoselov, S. V., Lobanov, A. V., Zehtab, O., Guigo, R., and Gladyshev, V. N.Characterization of Mammalian Selenoproteomes.Science.;300:1439-1443.