DF/HCC Program Affiliation

Cancer Immunology
Cancer Cell Biology

Research Abstract

Cell migration into inflamed sites is the result of a complex set of molecularly distinguishable signals. A family of chemoattractant cytokines, termed chemokines, released at the focus of tissue injury attract circulating leukocytes. Chemokines induce leukocyte migration and activation by binding to a family of G protein-coupled receptors that are differentiallyexpressed on various target cells. The expression of most chemokine receptors is restricted to leukocytes. In addition, some chemokine receptors are constitutively expressed while products of the inflammatory response regulate the expression of others. This laboratory has cloned and characterized several murine chemokines and chemokine receptors involved in physiologic and pathologic responses. Particular attention is focused on those chemokines that are expressed in the central nervous system, including those associated with chronic inflammatory diseases or cell survival. We have also used chemokines as a cancer therapeutic.

Publications

• Abromson-Leeman S, Ladell DS, Bronson RT, Dorf ME.Heterogeneity of EAE mediated by multiple distinct T-effector subsets.J Neuroimmunol 2007 Dec;192(1-2):3-12.
  17976744
• Li S, Wang L, Berman MA, Zhang Y, Dorf ME.RNAi screen in mouse astrocytes identifies phosphatases that regulate NF-kappaB signaling.Mol Cell 2006 Nov 17;24(4):497-509.
  17188031
• Sidorov A, Dorf M, Zorin V, Bokhanov A, Izotov I, Razin S, Skalyga V, Rossbach J, Spadtke P, Balabaev A.Multiaperture ion beam extraction from gas-dynamic electron cyclotron resonance source of multicharged ions.Rev Sci Instrum 2008 Feb;79(2 Pt 2):02A317.
  18315107
• Di Y, Li S, Wang L, Zhang Y, Dorf ME.Homeostatic interactions between MEKK3 and TAK1 involved in NF-kappaB signaling.Cell Signal 2008 Apr;20(4):705-13.
  18206350
• Xie JF, Wang G, Debonera F, Han R, Dorf ME, Hancock W, Olthoff KM.Selective neutralization of the chemokine TCA3 reduces the increased injury of partial versus whole liver transplants induced by cold preservation.Transplantation 2006 Dec 15;82(11):1501-9.
  17164723
• Lee I, Wang L, Wells AD, Dorf ME, Ozkaynak E, Hancock WW.Recruitment of Foxp3+ T regulatory cells mediating allograft tolerance depends on the CCR4 chemokine receptor.J Exp Med 2005 Apr 4;201(7):1037-44.
  15809349
• Zhai Q, Luo Y, Zhang Y, Berman MA, Dorf ME.Low nuclear levels of nuclear factor-kappa B are essential for KC self-induction in astrocytes: requirements for shuttling and phosphorylation.Glia 2004 Dec;48(4):327-36.
  15390109
• Luo Y, Berman MA, Abromson-Leeman SR, Dorf ME.Tumor necrosis factor is required for RANTES-induced astrocyte monocyte chemoattractant protein-1 production.Glia 2003 Aug;43(2):119-27.
  12838504
• Zhang Y, Luo Y, Zhai Q, Ma L, Dorf ME.Negative role of cAMP-dependent protein kinase A in RANTES-mediated transcription of proinflammatory mediators through Raf.FASEB J 2003 Apr;17(6):734-6.
  12586731
• Tanabe S, Nakadai T, Furuoka H, Oomachi T, Kobayashi Y, Omata Y, Koyama T, Hondo E, Uzuka Y, Sarashina T, Ducusin RJ, Shida T, Dorf ME.Expression of mRNA of chemokine receptor CXCR4 in feline mammary adenocarcinoma.Vet Rec 2002 Dec 14;151(24):729-33.
  12509077
• Luo Y, Berman MA, Zhai Q, Fischer FR, Abromson-Leeman SR, Zhang Y, Kuziel WA, Gerard C, Dorf ME.RANTES stimulates inflammatory cascades and receptor modulation in murine astrocytes.Glia 2002 Jul;39(1):19-30.
  12112372
• Zhang Y, Zhai Q, Luo Y, Dorf ME.RANTES-mediated chemokine transcription in astrocytes involves activation and translocation of p90 ribosomal S6 protein kinase (RSK).J Biol Chem 2002 May 24;277(21):19042-8.
  11893739
• Palframan RT, Jung S, Cheng G, Weninger W, Luo Y, Dorf M, Littman DR, Rollins BJ, Zweerink H, Rot A, von Andrian UH.Inflammatory chemokine transport and presentation in HEV: a remote control mechanism for monocyte recruitment to lymph nodes in inflamed ti
  11696600
• Fischer FR, Luo Y, Luo M, Santambrogio L, Dorf ME.RANTES-induced chemokine cascade in dendritic cells.J Immunol 2001 Aug 1;167(3):1637-43.
  11466387
• Abromson-Leeman S, Maverakis E, Bronson R, Dorf ME.CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalo
  11180118
• Stein JV, Rot A, Luo Y, Narasimhaswamy M, Nakano H, Gunn MD, Matsuzawa A, Quackenbush EJ, Dorf ME, von Andrian UH.The CC chemokine thymus-derived chemotactic agent 4 (TCA-4, secondary lymphoid tissue chemokine, 6Ckine, exodus-2) triggers lymphocyte functi
  10620605
• Luo Y, Lloyd C, Gutierrez-Ramos JC, Dorf ME.Chemokine amplification in mesangial cells.J Immunol 1999 Oct 1;163(7):3985-92.
  10491001
• Santambrogio L, Pakaski M, Wong ML, Cipriani B, Brosnan CF, Lees MB, Dorf ME.Antigen presenting capacity of brain microvasculature in altered peptide ligand modulation of experimental allergic encephalomyelitis.J Neuroimmunol 1999 Jan 1;93(1-2):81-91.
  10378871
• Luo Y, Kim R, Gabuzda D, Mi S, Collins-Racie LA, Lu Z, Jacobs KA, Dorf ME.The CXC-chemokine, H174: expression in the central nervous system.J Neurovirol 1998 Dec;4(6):575-85.
  10065899
• Yoshizawa I, Bronson R, Ben-Nun A, Richert JR, Dorf ME, Abromson-Leeman S.Differential recognition of MBP epitopes in BALB/c mice determines the site of inflammatory disease induction.J Neuroimmunol 1998 Aug 14;89(1-2):73-82.
  9726828
• Yoshizawa I, Bronson R, Dorf ME, Abromson-Leeman S.T-cell responses to myelin basic protein in normal and MBP-deficient mice.J Neuroimmunol 1998 Apr 15;84(2):131-8.
  9628454
• Heesen M, Berman MA, Charest A, Housman D, Gerard C, Dorf ME.Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1.Immunogenetics 1998 Apr;47(5):364-70.
  9510554
• Tanabe S, Heesen M, Berman MA, Fischer MB, Yoshizawa I, Luo Y, Dorf ME.Murine astrocytes express a functional chemokine receptor.J Neurosci 1997 Sep 1;17(17):6522-8.
  9254664
• Tanabe S, Heesen M, Yoshizawa I, Berman MA, Luo Y, Bleul CC, Springer TA, Okuda K, Gerard N, Dorf ME.Functional expression of the CXC-chemokine receptor-4/fusin on mouse microglial cells and astrocytes.J Immunol 1997 Jul 15;159(2):905-11.
  9218610
• Heesen M, Berman MA, Höpken UE, Gerard NP, Dorf ME.Alternate splicing of mouse fusin/CXC chemokine receptor-4: stromal cell-derived factor-1alpha is a ligand for both CXC chemokine receptor-4 isoforms.J Immunol 1997 Apr 15;158(8):3561-4.
  9103415