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Stephen C. Blacklow, MD, PhD

Publications

  • Wang H, Zang C, Taing L, Arnett KL, Wong YJ, Pear WS, Blacklow SC, Liu XS, Aster JC.NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions with superenhancers.Proc Natl Acad Sci U S A. 2014 Jan 14;111(2):705-10.
    24374627
  • Blacklow SC.Refining a Jagged edge.Structure. 2013 Dec 3;21(12):2100-1.
    24315457
  • Andrawes MB, Xu X, Liu H, Ficarro SB, Marto JA, Aster JC, Blacklow SC.Intrinsic selectivity of Notch 1 for Delta-like 4 over Delta-like 1.J Biol Chem. 2013 Aug 30;288(35):25477-89.
    23839946
  • Tiyanont K, Wales TE, Siebel CW, Engen JR, Blacklow SC.Insights into Notch3 activation and inhibition mediated by antibodies directed against its negative regulatory region.J Mol Biol. 2013 Sep 9;425(17):3192-204.
    23747483
  • Roti G, Carlton A, Ross KN, Markstein M, Pajcini K, Su AH, Perrimon N, Pear WS, Kung AL, Blacklow SC, Aster JC, Stegmaier K.Complementary genomic screens identify SERCA as a therapeutic target in NOTCH1 mutated cancer.Cancer Cell. 2013 Mar 18;23(3):390-405.
    23434461
  • Calderwood MA, Lee S, Holthaus AM, Blacklow SC, Kieff E, Johannsen E.Epstein-Barr virus nuclear protein 3C binds to the N-terminal (NTD) and beta trefoil domains (BTD) of RBP/CSL; Only the NTD interaction is essential for lymphoblastoid cell growth.Virology. 2011 Mar 25.
    21440926
  • Ashworth TD, Pear WS, Chiang MY, Blacklow SC, Mastio J, Xu L, Kelliher M, Kastner P, Chan S, Aster JC.Deletion-based mechanisms of Notch1 activation in T-ALL: key roles for RAG recombinase and a conserved internal translational start site in Notch1.Blood. 2010 Dec 16;116(25):5455-64.
    20852131
  • Keeshan K, Bailis W, Dedhia PH, Vega ME, Shestova O, Xu L, Toscano K, Uljon SN, Blacklow SC, Pear WS.Transformation by Tribbles homologue 2 (Trib2) requires both the Trib2 kinase domain and COP1 binding.Blood. 2010 Dec 2;116(23):4948-57.
    20805362
  • Del Bianco C, Vedenko A, Choi SH, Berger MF, Shokri L, Bulyk ML, Blacklow SC.Notch and MAML-1 complexation do not detectably alter the dna binding specificity of the transcription factor CSL.PLoS ONE. 2010;5(11):e15034.
    21124806
  • Aster JC, Blacklow SC, Pear WS.Notch signalling in T-cell lymphoblastic leukaemia/lymphoma and other haematological malignancies.J Pathol. 2011 Jan;223(2):262-73.
    20967796
  • Arnett KL, Hass M, McArthur DG, Ilagan MX, Aster JC, Kopan R, Blacklow SC.Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes.Nat Struct Mol Biol. 2010 Nov;17(11):1312-7.
    20972443
  • Liu H, Chi AW, Arnett KL, Chiang MY, Xu L, Shestova O, Wang H, Li YM, Bhandoola A, Aster JC, Blacklow SC, Pear WS.Notch dimerization is required for leukemogenesis and T-cell development.Genes Dev. 2010 Nov 1;24(21):2395-407.
    20935071
  • Kovall RA, Blacklow SC.Mechanistic insights into Notch receptor signaling from structural and biochemical studies.Curr Top Dev Biol. 2010;92:31-71.
    20816392
  • Dedhia PH, Keeshan K, Uljon S, Xu L, Vega ME, Shestova O, Zaks-Zilberman M, Romany C, Blacklow SC, Pear WS.Differential ability of Tribbles family members to promote degradation of C/EBP{alpha} and induce acute myelogenous leukemia.Blood. 2010 Aug 26;116(8):1321-8.
    20410507
  • Aste-Am├ęzaga M, Zhang N, Lineberger JE, Arnold BA, Toner TJ, Gu M, Huang L, Vitelli S, Vo KT, Haytko P, Zhao JZ, Baleydier F, L'Heureux S, Wang H, Gordon WR, Thoryk E, Andrawes MB, Tiyanont K, Stegmaier K, Roti G, Ross KN, Franlin LL, Wang H, Wang F, Chastain M, Bett AJ, Audoly LP, Aster JC, Blacklow SC, Huber HE.Characterization of Notch1 antibodies that inhibit signaling of both normal and mutated Notch1 receptors.PLoS ONE. 2010;5(2):e9094.
    20161710
  • Moellering RE, Cornejo M, Davis TN, Del Bianco C, Aster JC, Blacklow SC, Kung AL, Gilliland DG, Verdine GL, Bradner JE.Direct inhibition of the NOTCH transcription factor complex.Nature. 2009 Nov 12;462(7270):182-8.
    19907488
  • Moellering RE, Cornejo M, Davis TN, Del Bianco C, Aster JC, Blacklow SC, Kung AL, Gilliland DG, Verdine GL, Bradner J.Direct inhibition of the Notch transcription factor complex.Nature..
  • Gordon WR, Vardar-Ulu D, L'Heureux S, Ashworth T, Malecki MJ, Sanchez-Irizarry C, McArthur DG, Histen G, Mitchell JL, Aster JC, Blacklow SC.Effects of S1 cleavage on the structure, surface export, and signaling activity of human Notch1 and Notch2.PLoS ONE. 2009;4(8):e6613.
    19701457
  • Yashiro-Ohtani Y, He Y, Ohtani T, Jones ME, Shestova O, Xu L, Fang TC, Chiang MY, Intlekofer AM, Blacklow SC, Zhuang Y, Pear WS.Pre-TCR signaling inactivates Notch1 transcription by antagonizing E2A.Genes Dev. 2009 Jul 15;23(14):1665-76.
    19605688
  • Gordon WR,Roy M,Vardar-Ulu D,Garfinkel M,Mansour MR,Aster JC,Blacklow SC.Structure of the Notch1-negative regulatory region: implications for normal activation and pathogenic signaling in T-ALL.Blood. 2009 Apr 30;113(18):4381-90.
    19075186
  • Gordon WR,Arnett KL,Blacklow SC.The molecular logic of Notch signaling--a structural and biochemical perspective.J Cell Sci. 2008 Oct 1;121(Pt 19):3109-19. Review.
    18799787
  • Li K, Li Y, Wu W, Gordon WR, Chang DW, Lu M, Scoggin S, Fu T, Vien L, Histen G, Zheng J, Martin-Hollister R, Duensing T, Singh S, Blacklow SC, Yao Z, Aster JC, Zhou BB.Modulation of Notch signaling by antibodies specific for the extracellular negative regulatory region of NOTCH3.J Biol Chem. 2008 Mar 21;283(12):8046-54.
    18182388
  • Estrada K, Fisher C, Blacklow SC.Unfolding of the RAP-D3 helical bundle facilitates dissociation of RAP-receptor complexes.Biochemistry. 2008 Feb 12;47(6):1532-9.
    18177055
  • Del Bianco C, Aster JC, Blacklow SC.Mutational and energetic studies of Notch 1 transcription complexes.J Mol Biol. 2008 Feb 8;376(1):131-40.
    18155729
  • Aster JC, Pear WS, Blacklow SC.Notch signaling in leukemia.Annu Rev Pathol. 2008;3:587-613. Review.
    18039126
  • Blacklow SC.Versatility in ligand recognition by LDL receptor family proteins: advances and frontiers.Curr Opin Struct Biol. 2007 Aug;17(4):419-26. Review.
    17870468
  • Fang TC, Yashiro-Ohtani Y, Del Bianco C, Knoblock DM, Blacklow SC, Pear WS.Notch directly regulates Gata3 expression during T helper 2 cell differentiation.Immunity. 2007 Jul;27(1):100-10.
    17658278
  • Koduri V, Blacklow SC.Requirement for natively unstructured regions of mesoderm development candidate 2 in promoting low-density lipoprotein receptor-related protein 6 maturation.Biochemistry. 2007 Jun 5;46(22):6570-7.
    17488095
  • Gordon WR, Vardar-Ulu D, Histen G, Sanchez-Irizarry C, Aster JC, Blacklow SC.Corrigendum: Structural basis for autoinhibition of Notch.Nat Struct Mol Biol. 2007 May;14(5):455.
    17473881
  • Gordon WR, Vardar-Ulu D, Histen G, Sanchez-Irizarry C, Aster JC, Blacklow SC.Structural basis for autoinhibition of Notch.Nat Struct Mol Biol. 2007 Apr;14(4):295-300.
    17401372
  • Nam Y, Sliz P, Pear WS, Aster JC, Blacklow SC.Cooperative assembly of higher-order Notch complexes functions as a switch to induce transcription.Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2103-8.
    17284587
  • Weng AP, Millholland JM, Yashiro-Ohtani Y, Arcangeli ML, Lau A, Wai C, Del Bianco C, Rodriguez CG, Sai H, Tobias J, Li Y, Wolfe MS, Shachaf C, Felsher D, Blacklow SC, Pear WS, Aster.c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphoma.Genes Dev. 2006 Aug 1;20(15):2096-109.
    16847353
  • Chiang MY, Xu ML, Histen G, Shestova O, Roy M, Nam Y, Blacklow SC, Sacks DB, Pear WS, Aster JC.Identification of a Conserved Negative Regulatory Sequence That Influences the Leukemogenic Activity of NOTCH1.Mol Cell Biol. 2006 Aug;26(16):6261-71.
    16880534
  • Malecki MJ, Sanchez-Irizarry C, Mitchell JL, Histen G, Xu ML, Aster JC, Blacklow SC.Leukemia-associated mutations within the NOTCH1 heterodimerization domain fall into at least two distinct mechanistic classes.Mol Cell Biol. 2006 Jun;26(12):4642-51.
    16738328
  • Lee D, Walsh JD, Mikhailenko I, Yu P, Migliorini M, Wu Y, Krueger S, Curtis JE, Harris B, Lockett S, Blacklow SC, Strickland DK, Wang YX.RAP uses a histidine switch to regulate its interaction with LRP in the ER and Golgi.Mol Cell. 2006 May 5;22(3):423-30.
    16678114
  • Fisher C, Beglova N, Blacklow SC.Structure of an LDLR-RAP complex reveals a general mode for ligand recognition by lipoprotein receptors.Mol Cell. 2006 Apr 21;22(2):277-83.
    16630895
  • Nam Y, Sliz P, Song L, Aster JC, Blacklow SC.Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.Cell. 2006 Mar 10;124(5):973-83.
    16530044
  • Blacklow SC.A new niche for notch on deltex?.Structure Fold Des. 2005 Nov;13(11):1579-80.
    16271878
  • Tan Z, Blacklow SC, Cornish VW, Forster AC.De novo genetic codes and pure translation display.Methods. 2005 Jul;36(3):279-90.
    16076454
  • Jeon H, Blacklow SC.Structure and physiologic function of the low-density lipoprotein receptor.Annu Rev Biochem. 2005;74:535-62.
    15952897
  • Beglova N, Blacklow SC.The LDL receptor: how acid pulls the trigger.Trends Biochem Sci. 2005 Jun;30(6):309-17.
    15950875
  • Abdul-Aziz D, Fisher C, Beglova N, Blacklow SC.Folding and binding integrity of variants of a prototype ligand-binding module from the LDL receptor possessing multiple alanine substitutions.Biochemistry. 2005 Apr 5;44(13):5075-85.
    15794645
  • Stolt PC, Chen Y, Liu P, Bock HH, Blacklow SC, Herz J.Phosphoinositide binding by the disabled-1 PTB domain is necessary for membrane localization and Reelin signal transduction.J Biol Chem. 2005 Mar 11;280(10):9671-7.
    15632144
  • Beglova N, Jeon H, Fisher C, Blacklow SC.Structural features of the low-density lipoprotein receptor facilitating ligand binding and release.Biochem Soc Trans. 2004 Nov;32(Pt 5):721-3.
    15493997
  • Beglova N, Jeon H, Fisher C, Blacklow SC.Cooperation between fixed and low pH-inducible interfaces controls lipoprotein release by the LDL receptor.Mol Cell. 2004 Oct 22;16(2):281-92.
    15494314
  • Sanchez-Irizarry C, Carpenter AC, Weng AP, Pear WS, Aster JC, Blacklow SC.Notch subunit heterodimerization and prevention of ligand-independent proteolytic activation depend, respectively, on a novel domain and the LNR repeats.Mol Cell Biol. 2004 Nov;24(21):9265-73.
    15485896
  • Weng AP, Ferrando AA, Lee W, Morris JP, Silverman LB, Sanchez-Irizarry C, Blacklow SC, Look AT, Aster JC.Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.Science. 2004 Oct 8;306(5694):269-71.
    15472075
  • Tan Z, Forster AC, Blacklow SC, Cornish VW.Amino acid backbone specificity of the Escherichia coli translation machinery.J Am Chem Soc. 2004 Oct 13;126(40):12752-3.
    15469251
  • Forster AC, Cornish VW, Blacklow SC.Pure translation display.Anal Biochem. 2004 Oct 15;333(2):358-64.
    15450813
  • Stolt PC, Vardar D, Blacklow SC.The dual-function disabled-1 PTB domain exhibits site independence in binding phosphoinositide and peptide ligands.Biochemistry. 2004 Aug 31;43(34):10979-87.
    15323557
  • Blacklow SC.Catching the common cold.Nat Struct Mol Biol. 2004 May;11(5):388-90.
    15114337
  • Boswell EJ, Jeon H, Blacklow SC, Downing AK.Global defects in the expression and function of the low density lipoprotein receptor (LDLR) associated with two familial hypercholesterolemia mutations resulting in misfolding of the LDLR epidermal growth factor-AB pair.J Biol Chem. 2004 Jul 16;279(29):30611-21.
    15100232
  • Fisher C, Abdul-Aziz D, Blacklow SC.A two-module region of the low-density lipoprotein receptor sufficient for formation of complexes with apolipoprotein E ligands.Biochemistry. 2004 Feb 3;43(4):1037-44.
    14744149
  • Smith JG, Mothes W, Blacklow SC, Cunningham JM.The mature avian leukosis virus subgroup A envelope glycoprotein is metastable, and refolding induced by the synergistic effects of receptor binding and low pH is coupled to infection.J Virol. 2004 Feb;78(3):1403-10.
    14722295
  • Kim PW, Sun ZY, Blacklow SC, Wagner G, Eck MJ.A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8.Science. 2003 Sep 19;301(5640):1725-8.
    14500983
  • Vardar D, North CL, Sanchez-Irizarry C, Aster JC, Blacklow SC.Nuclear magnetic resonance structure of a prototype Lin12-Notch repeat module from human Notch1.Biochemistry. 2003 Jun 17;42(23):7061-7.
    12795601
  • Forster AC, Tan Z, Nalam MN, Lin H, Qu H, Cornish VW, Blacklow SC.Programming peptidomimetic syntheses by translating genetic codes designed de novo.Proc Natl Acad Sci U S A. 2003 May 27;100(11):6353-7.
    12754376
  • Stolt PC, Jeon H, Song HK, Herz J, Eck MJ, Blacklow SC.Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes.Structure Fold Des. 2003 May;11(5):569-79.
    12737822
  • Nam Y, Weng AP, Aster JC, Blacklow SC.Structural requirements for assembly of the CSL.intracellular Notch1.Mastermind-like 1 transcriptional activation complex.J Biol Chem. 2003 Jun 6;278(23):21232-9.
    12644465
  • Jeon H, Blacklow SC.An intramolecular spin of the LDL receptor beta propeller.Structure Fold Des. 2003 Feb;11(2):133-6.
    12575931
  • Weng AP, Nam Y, Wolfe MS, Pear WS, Griffin JD, Blacklow SC, Aster JC.Growth suppression of pre-T acute lymphoblastic leukemia cells by inhibition of notch signaling.Mol Cell Biol. 2003 Jan;23(2):655-64.
    12509463
  • Nam Y, Aster JC, Blacklow SC.Notch signaling as a therapeutic target.Curr Opin Chem Biol. 2002 Aug;6(4):501-9.
    12133727
  • Beglova N, Blacklow SC, Takagi J, Springer TA.Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation.Nat Struct Biol. 2002 Apr;9(4):282-7.
    11896403
  • Stone JR, Maki JL, Blacklow SC, Collins T.The SCAN domain of ZNF174 is a dimer.J Biol Chem. 2002 Feb 15;277(7):5448-52.
    11741982
  • Koduri V, Blacklow SC.Folding determinants of LDL receptor type A modules.Biochemistry. 2001 Oct 30;40(43):12801-7.
    11669616
  • Takagi J, Beglova N, Yalamanchili P, Blacklow SC, Springer TA.Definition of EGF-like, closely interacting modules that bear activation epitopes in integrin beta subunits.Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11175-80.
    11572973
  • Forster AC, Weissbach H, Blacklow SC.A simplified reconstitution of mRNA-directed peptide synthesis: activity of the epsilon enhancer and an unnatural amino acid.Anal Biochem. 2001 Oct 1;297(1):60-70.
    11567528
  • Jeon H, Meng W, Takagi J, Eck MJ, Springer TA, Blacklow SC.Implications for familial hypercholesterolemia from the structure of the LDL receptor YWTD-EGF domain pair.Nat Struct Biol. 2001 Jun;8(6):499-504.
    11373616
  • Adkins HB, Blacklow SC, Young JA.Two functionally distinct forms of a retroviral receptor explain the nonreciprocal receptor interference among subgroups B, D, and E avian leukosis viruses.J Virol. 2001 Apr;75(8):3520-6.
    11264341
  • Beglova N, North CL, Blacklow SC.Backbone dynamics of a module pair from the ligand-binding domain of the LDL receptor.Biochemistry. 2001 Mar 6;40(9):2808-15.
    11258891
  • Wu L, Aster JC, Blacklow SC, Lake R, Artavanis-Tsakonas S, Griffin JD.MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors.Nat Genet. 2000 Dec;26(4):484-9.
    11101851
  • North CL, Blacklow SC.Evidence that familial hypercholesterolemia mutations of the LDL receptor cause limited local misfolding in an LDL-A module pair.Biochemistry. 2000 Oct 31;39(43):13127-35.
    11052664
  • North CL, Blacklow SC.Solution structure of the sixth LDL-A module of the LDL receptor.Biochemistry. 2000 Mar 14;39(10):2564-71.
    10704205
  • Rand MD, Grimm LM, Artavanis-Tsakonas S, Patriub V, Blacklow SC, Sklar J, Aster JC.Calcium depletion dissociates and activates heterodimeric notch receptors.Mol Cell Biol. 2000 Mar;20(5):1825-35.
    10669757
  • Williams AJ, Blacklow SC, Collins T.The zinc finger-associated SCAN box is a conserved oligomerization domain.Mol Cell Biol. 1999 Dec;19(12):8526-35.
    10567577
  • Aster JC, Simms WB, Zavala-Ruiz Z, Patriub V, North CL, Blacklow SC.The folding and structural integrity of the first LIN-12 module of human Notch1 are calcium-dependent.Biochemistry. 1999 Apr 13;38(15):4736-42.
    10200161
  • North CL, Blacklow SC.Structural independence of ligand-binding modules five and six of the LDL receptor.Biochemistry. 1999 Mar 30;38(13):3926-35.
    10194304
  • Fass D, Blacklow S, Kim PS, Berger JM.Molecular basis of familial hypercholesterolaemia from structure of LDL receptor module.Nature. 1997 Aug 14;388(6643):691-3.
    9262405
  • Blacklow SC, Kim PS.Protein folding and calcium binding defects arising from familial hypercholesterolemia mutations of the LDL receptor.Nat Struct Biol. 1996 Sep;3(9):758-62.
    8784348
  • Lu M, Blacklow SC, Kim PS.A trimeric structural domain of the HIV-1 transmembrane glycoprotein.Nat Struct Biol. 1995 Dec;2(12):1075-82.
    8846219
  • Blacklow SC, Lu M, Kim PS.A trimeric subdomain of the simian immunodeficiency virus envelope glycoprotein.Biochemistry. 1995 Nov 21;34(46):14955-62.
    7578108
  • Blacklow SC, Knowles JR.How can a catalytic lesion be offset? The energetics of two pseudorevertant triosephosphate isomerases.Biochemistry. 1990 May 1;29(17):4099-108.
    2361134
  • Hermes JD, Blacklow SC, Knowles JR.Searching sequence space by definably random mutagenesis: improving the catalytic potency of an enzyme.Proc Natl Acad Sci U S A. 1990 Jan;87(2):696-700.
    1967829
  • Hermes JD, Parekh SM, Blacklow SC, K÷ster H, Knowles JR.A reliable method for random mutagenesis: the generation of mutant libraries using spiked oligodeoxyribonucleotide primers.Gene. 1989 Dec 7;84(1):143-51.
    2691332
  • Blacklow SC, Raines RT, Lim WA, Zamore PD, Knowles JR.Triosephosphate isomerase catalysis is diffusion controlled. Appendix: Analysis of triose phosphate equilibria in aqueous solution by 31P NMR.Biochemistry. 1988 Feb 23;27(4):1158-67.
    3365378
  • Hermes JD, Blacklow SC, Knowles JR.The development of enzyme catalytic efficiency: an experimental approach.Cold Spring Harb Symp Quant Biol. 1987;52:597-602.
    3454280