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John Blenis, PhD


  • Yi T, Zhai B, Yu Y, Kiyotsugu Y, Raschle T, Etzkorn M, Seo HC, Nagiec M, Luna RE, Reinherz EL, Blenis J, Gygi SP, Wagner G.Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells.Proc Natl Acad Sci U S A. 2014 May 27;111(21):E2182-90.
  • Liu M, Bai J, He S, Villarreal R, Hu D, Zhang C, Yang X, Liang H, Slaga TJ, Yu Y, Zhou Z, Blenis J, Scherer PE, Dong LQ, Liu F.Grb10 Promotes Lipolysis and Thermogenesis by Phosphorylation-Dependent Feedback Inhibition of mTORC1.Cell Metab. 2014 Apr 15.
  • Li J, Kim SG, Blenis J.Rapamycin: one drug, many effects.Cell Metab. 2014 Mar 4;19(3):373-9.
  • Lee G, Blenis J.Akt-ivation of RNA splicing.Mol Cell. 2014 Feb 20;53(4):519-20.
  • Li C, Lee PS, Sun Y, Gu X, Zhang E, Guo Y, Wu CL, Auricchio N, Priolo C, Li J, Csibi A, Parkhitko A, Morrison T, Planaguma A, Kazani S, Israel E, Xu KF, Henske EP, Blenis J, Levy BD, Kwiatkowski D, Yu JJ.Estradiol and mTORC2 cooperate to enhance prostaglandin biosynthesis and tumorigenesis in TSC2-deficient LAM cells.J Exp Med. 2014 Jan 13;211(1):15-28.
  • Liu P, Gan W, Inuzuka H, Lazorchak AS, Gao D, Arojo O, Liu D, Wan L, Zhai B, Yu Y, Yuan M, Kim BM, Shaik S, Menon S, Gygi SP, Lee TH, Asara JM, Manning BD, Blenis J, Su B, Wei W.Sin1 phosphorylation impairs mTORC2 complex integrity and inhibits downstream Akt signalling to suppress tumorigenesis.Nat Cell Biol. 2013 Nov;15(11):1340-50.
  • Gu X, Yu JJ, Ilter D, Blenis N, Henske EP, Blenis J.Integration of mTOR and estrogen-ERK2 signaling in lymphangioleiomyomatosis pathogenesis.Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):14960-5.
  • Fendt SM, Bell EL, Keibler MA, Davidson SM, Wirth GJ, Fiske B, Mayers JR, Schwab M, Bellinger G, Csibi A, Patnaik A, Blouin MJ, Cantley LC, Guarente L, Blenis J, Pollak MN, Olumi AF, Vander Heiden MG, Stephanopoulos G.Metformin decreases glucose oxidation and increases the dependency of prostate cancer cells on reductive glutamine metabolism.Cancer Res. 2013 Jul 15;73(14):4429-38.
  • Kim SG, Buel GR, Blenis J.Nutrient regulation of the mTOR complex 1 signaling pathway.Mol Cells. 2013 Jun;35(6):463-73.
  • Er EE, Mendoza MC, Mackey AM, Rameh LE, Blenis J.AKT facilitates EGFR trafficking and degradation by phosphorylating and activating PIKfyve.Sci Signal. 2013 Jun 11;6(279):ra45.
  • Csibi A, Fendt SM, Li C, Poulogiannis G, Choo AY, Chapski DJ, Jeong SM, Dempsey JM, Parkhitko A, Morrison T, Henske EP, Haigis MC, Cantley LC, Stephanopoulos G, Yu J, Blenis J.The mTORC1 pathway stimulates glutamine metabolism and cell proliferation by repressing SIRT4.Cell. 2013 May 9;153(4):840-54.
  • Jeong SM, Xiao C, Finley LW, Lahusen T, Souza AL, Pierce K, Li YH, Wang X, Laurent G, German NJ, Xu X, Li C, Wang RH, Lee J, Csibi A, Cerione R, Blenis J, Clish CB, Kimmelman A, Deng CX, Haigis MC.SIRT4 has tumor-suppressive activity and regulates the cellular metabolic response to DNA damage by inhibiting mitochondrial glutamine metabolism.Cancer Cell. 2013 Apr 15;23(4):450-63.
  • Kim SG, Hoffman GR, Poulogiannis G, Buel GR, Jang YJ, Lee KW, Kim BY, Erikson RL, Cantley LC, Choo AY, Blenis J.Metabolic Stress Controls mTORC1 Lysosomal Localization and Dimerization by Regulating the TTT-RUVBL1/2 Complex.Mol Cell. 2012 Nov 6.
  • Anjum R, Pae E, Blenis J, Ballif BA.TPCK inhibits AGC kinases by direct activation loop adduction at phenylalanine-directed cysteine residues.FEBS Lett. 2012 Sep 21;586(19):3471-6.
  • Mendoza MC, Er EE, Zhang W, Ballif BA, Elliott HL, Danuser G, Blenis J.ERK-MAPK Drives Lamellipodia Protrusion by Activating the WAVE2 Regulatory Complex.Mol Cell. 2011 Mar 18;41(6):661-71.
  • Kim SG, Choo AY, Blenis J.ATM: Promoter of metabolic "cost" reduction and "savings" usage during hypoxia through mTORC1 regulation.Mol Cell. 2010 Nov 24;40(4):501-2.
  • Mendoza MC, Er EE, Blenis J.ERK-MAP Kinase signaling in the cytoplasm.Methods Mol Biol. 2010;661:185-203.
  • Lipinski MM, Hoffman G, Ng A, Zhou W, Py BF, Hsu E, Liu X, Eisenberg J, Liu J, Blenis J, Xavier RJ, Yuan J.A genome-wide siRNA screen reveals multiple mTORC1 independent signaling pathways regulating autophagy under normal nutritional conditions.Dev Cell. 2010 Jun 15;18(6):1041-52.
  • Shin S, Blenis J.ERK2/Fra1/ZEB pathway induces epithelial-to-mesenchymal transition.Cell Cycle. 2010 Jul 7;9(13).
  • Choo AY, Kim SG, Vander Heiden MG, Mahoney SJ, Vu H, Yoon SO, Cantley LC, Blenis J.Glucose addiction of TSC null cells is caused by failed mTORC1-dependent balancing of metabolic demand with supply.Mol Cell. 2010 May 28;38(4):487-99.
  • Hoffman GR, Moerke NJ, Hsia M, Shamu CE, Blenis J.A High-Throughput, Cell-Based Screening Method for siRNA and Small Molecule Inhibitors of mTORC1 Signaling Using the In Cell Western Technique.Assay And Drug Development Technologies. 2010 Apr;8(2):186-99.
  • Shin S, Dimitri CA, Yoon SO, Dowdle W, Blenis J.ERK2 but not ERK1 induces epithelial-to-mesenchymal transformation via DEF motif-dependent signaling events.Mol Cell. 2010 Apr 9;38(1):114-27.
  • Mahoney SJ, Dempsey JM, Blenis J.Chapter 2 Cell Signaling in Protein Synthesis Ribosome Biogenesis and Translation Initiation and Elongation.Progress In Molecular Biology And Translational Science. 2009;90C:53-107.
  • Ma XM,Blenis J.Molecular mechanisms of mTOR-mediated translational control.Nat Rev Mol Cell Biol. 2009 May;10(5):307-18. Review.
  • Abe Y,Yoon SO,Kubota K,Mendoza MC,Gygi SP,Blenis J.p90 Ribosomal S6 Kinase and p70 Ribosomal S6 Kinase Link Phosphorylation of the Eukaryotic Chaperonin Containing TCP-1 to Growth Factor, Insulin, and Nutrient Signaling.J Biol Chem. 2009 May 29;284(22):14939-48.
  • Choo AY,Blenis J.Not all substrates are treated equally: implications for mTOR, rapamycin-resistance and cancer therapy.Cell Cycle. 2009 Feb 15;8(4):567-72. Review.
  • Rahimi RA,Andrianifahanana M,Wilkes MC,Edens M,Kottom TJ,Blenis J,Leof EB.Distinct roles for mammalian target of rapamycin complexes in the fibroblast response to transforming growth factor-beta.Cancer Res. 2009 Jan 1;69(1):84-93.
  • Choo AY,Yoon SO,Kim SG,Roux PP,Blenis J.Rapamycin differentially inhibits S6Ks and 4E-BP1 to mediate cell-type-specific repression of mRNA translation.Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17414-9.
  • Anjum R,Blenis J.The RSK family of kinases: emerging roles in cellular signalling.Nat Rev Mol Cell Biol. 2008 Oct;9(10):747-58. Review.
  • Zhu J, Blenis J, Yuan J.Activation of PI3K/Akt and MAPK pathways regulates Myc-mediated transcription by phosphorylating and promoting the degradation of Mad1.Proc Natl Acad Sci U S A. 2008 May 6;105(18):6584-9.
  • Ma XM, Yoon SO, Richardson CJ, Julich K, Blenis J.SKAR links pre-mRNA splicing to mTOR/S6K1-mediated enhanced translation efficiency of spliced mRNAs.Cell. 2008 Apr 18;133(2):303-13.
  • Yoon SO, Shin S, Liu Y, Ballif BA, Woo MS, Gygi SP, Blenis J.Ran-Binding Protein 3 Phosphorylation Links the Ras and PI3-Kinase Pathways to Nucleocytoplasmic Transport.Mol Cell. 2008 Feb 15;29(3):362-75.
  • Mendoza MC, Blenis J.PHLPPing it off: phosphatases get in the Akt.Mol Cell. 2007 Mar 23;25(6):798-800.
  • Roux PP, Shahbazian D, Vu H, Holz MK, Cohen MS, Taunton J, Sonenberg N, Blenis J.RAS/ERK signaling promotes site-specific ribosomal protein S6 phosphorylation via RSK and stimulates cap-dependent translation.J Biol Chem. 2007 May 11;282(19):14056-64.
  • Zito CI, Qin H, Blenis J, Bennett AM.SHP-2 regulates cell growth by controlling the mTOR/S6 kinase 1 pathway.J Biol Chem. 2007 Mar 9;282(10):6946-53.
  • Liu L, Li F, Cardelli JA, Martin KA, Blenis J, Huang S.Rapamycin inhibits cell motility by suppression of mTOR-mediated S6K1 and 4E-BP1 pathways.Oncogene. 2006 Nov 9;25(53):7029-40.
  • Choo AY, Roux PP, Blenis J.Mind the GAP: Wnt steps onto the mTORC1 train.Cell. 2006 Sep 8;126(5):834-6.
  • Shahbazian D, Roux PP, Mieulet V, Cohen MS, Raught B, Taunton J, Hershey JW, Blenis J, Pende M, Sonenberg N.The mTOR/PI3K and MAPK pathways converge on eIF4B to control its phosphorylation and activity.EMBO J. 2006 Jun 21;25(12):2781-91.
  • Kim M, Lee JH, Koh H, Lee SY, Jang C, Chung CJ, Sung JH, Blenis J, Chung J.Inhibition of ERK-MAP kinase signaling by RSK during Drosophila development.EMBO J. 2006 Jul 12;25(13):3056-67.
  • Hanrahan J, Blenis J.Rheb Activation of mTOR and S6K1 Signaling.Methods Enzymol. 2005;407:542-55.
  • Murphy LO, Blenis J.MAPK signal specificity: the right place at the right time.Trends Biochem Sci. 2006 May;31(5):268-75. Review.
  • Choo AY, Blenis J.TORgeting oncogene addiction for cancer therapy.Cancer Cell. 2006 Feb;9(2):77-9.
  • Lewthwaite JC, Bastow ER, Lamb KJ, Blenis J, Wheeler-Jones CP, Pitsillides AA.A specific mechanomodulatory role for p38 MAPK in embryonic joint articular surface cell MEK-ERK pathway regulation.J Biol Chem. 2006 Apr 21;281(16):11011-8.
  • Holz MK, Ballif BA, Gygi SP, Blenis J.mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events.Cell. 2005 Nov 18;123(4):569-80.
  • Anjum R, Roux PP, Ballif BA, Gygi SP, Blenis J.The Tumor Suppressor DAP Kinase Is a Target of RSK-Mediated Survival Signaling.Curr Biol. 2005 Oct 11;15(19):1762-7.
  • Tee AR, Blenis J, Proud CG.Analysis of mTOR signaling by the small G-proteins, Rheb and RhebL1.FEBS Lett. 2005 Aug 29;579(21):4763-8.
  • Dimitri CA, Dowdle W, MacKeigan JP, Blenis J, Murphy LO.Spatially separate docking sites on ERK2 regulate distinct signaling events in vivo.Curr Biol. 2005 Jul 26;15(14):1319-24.
  • Holz MK, Blenis J.Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase.J Biol Chem. 2005 Jul 15;280(28):26089-93.
  • Mackeigan JP, Murphy LO, Dimitri CA, Blenis J.Graded mitogen-activated protein kinase activity precedes switch-like c-Fos induction in mammalian cells.Mol Cell Biol. 2005 Jun;25(11):4676-82.
  • MacKeigan JP, Murphy LO, Blenis J.Sensitized RNAi screen of human kinases and phosphatases identifies new regulators of apoptosis and chemoresistance.Nat Cell Biol. 2005 Jun;7(6):591-600.
  • Tee AR, Blenis J.mTOR, translational control and human disease.Semin Cell Dev Biol. 2005 Feb;16(1):29-37.
  • Schalm SS, Tee AR, Blenis J.Characterization of a conserved C-terminal motif (RSPRR) in ribosomal protein S6 kinase 1 required for its mammalian target of rapamycin-dependent regulation.J Biol Chem. 2005 Mar 25;280(12):11101-6.
  • Ballif BA, Roux PP, Gerber SA, MacKeigan JP, Blenis J, Gygi SP.Quantitative phosphorylation profiling of the ERK/p90 ribosomal S6 kinase-signaling cassette and its targets, the tuberous sclerosis tumor suppressors.Proc Natl Acad Sci U S A. 2005 Jan 18;102(3):667-72.
  • Roux PP, Ballif BA, Anjum R, Gygi SP, Blenis J.Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase.Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13489-94.
  • Richardson CJ, Bröenstrup M, Fingar DC, Jülich K, Ballif BA, Gygi S, Blenis J.SKAR is a specific target of S6 kinase 1 in cell growth control.Curr Biol. 2004 Sep 7;14(17):1540-9.
  • Richardson CJ, Schalm SS, Blenis J.PI3-kinase and TOR: PIKTORing cell growth.Semin Cell Dev Biol. 2004 Apr;15(2):147-59.
  • Roux PP, Blenis J.ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions.Microbiol Mol Biol Rev. 2004 Jun;68(2):320-44.
  • Tee AR, Tee JA, Blenis J.Characterizing the interaction of the mammalian eIF4E-related protein 4EHP with 4E-BP1.FEBS Lett. 2004 Apr 23;564(1-2):58-62.
  • Fingar DC, Blenis J.Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression.Oncogene. 2004 Apr 19;23(18):3151-71.
  • Woo MS, Ohta Y, Rabinovitz I, Stossel TP, Blenis J.Ribosomal S6 kinase (RSK) regulates phosphorylation of filamin A on an important regulatory site.Mol Cell Biol. 2004 Apr;24(7):3025-35.
  • Murphy LO, MacKeigan JP, Blenis J.A network of immediate early gene products propagates subtle differences in mitogen-activated protein kinase signal amplitude and duration.Mol Cell Biol. 2004 Jan;24(1):144-53.
  • Fingar DC, Richardson CJ, Tee AR, Cheatham L, Tsou C, Blenis J.mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E.Mol Cell Biol. 2004 Jan;24(1):200-16.
  • Tee AR, Manning BD, Roux PP, Cantley LC, Blenis J.Tuberous sclerosis complex gene products, Tuberin and Hamartin, control mTOR signaling by acting as a GTPase-activating protein complex toward Rheb.Curr Biol. 2003 Aug 5;13(15):1259-68.
  • Tee AR, Anjum R, Blenis J.Inactivation of the tuberous sclerosis complex-1 and -2 gene products occurs by phosphoinositide 3-kinase/Akt-dependent and -independent phosphorylation of tuberin.J Biol Chem. 2003 Sep 26;278(39):37288-96.
  • Roux PP, Richards SA, Blenis J.Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity.Mol Cell Biol. 2003 Jul;23(14):4796-804.
  • Schalm SS, Fingar DC, Sabatini DM, Blenis J.TOS motif-mediated raptor binding regulates 4E-BP1 multisite phosphorylation and function.Curr Biol. 2003 May 13;13(10):797-806.
  • Martin KA, Blenis J.Coordinate regulation of translation by the PI 3-kinase and mTOR pathways.Adv Cancer Res. 2002;86:1-39.
  • Tee AR, Fingar DC, Manning BD, Kwiatkowski DJ, Cantley LC, Blenis J.Tuberous sclerosis complex-1 and -2 gene products function together to inhibit mammalian target of rapamycin (mTOR)-mediated downstream signaling.Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13571-6.
  • Romanelli A, Dreisbach VC, Blenis J.Characterization of phosphatidylinositol 3-kinase-dependent phosphorylation of the hydrophobic motif site Thr(389) in p70 S6 kinase 1.J Biol Chem. 2002 Oct 25;277(43):40281-9.
  • Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC.Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway.Mol Cell. 2002 Jul;10(1):151-62.
  • Murphy LO, Smith S, Chen RH, Fingar DC, Blenis J.Molecular interpretation of ERK signal duration by immediate early gene products.Nat Cell Biol. 2002 Aug;4(8):556-64.
  • Fingar DC, Salama S, Tsou C, Harlow E, Blenis J.Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E.Genes Dev. 2002 Jun 15;16(12):1472-87.
  • Schalm SS, Blenis J.Identification of a conserved motif required for mTOR signaling.Curr Biol. 2002 Apr 16;12(8):632-9.
  • Richards SA, Dreisbach VC, Murphy LO, Blenis J.Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1.Mol Cell Biol. 2001 Nov;21(21):7470-80.
  • Ballif BA, Blenis J.Molecular mechanisms mediating mammalian mitogen-activated protein kinase (MAPK) kinase (MEK)-MAPK cell survival signals.Cell Growth Differ. 2001 Aug;12(8):397-408.
  • Ballif BA, Shimamura A, Pae E, Blenis J.Disruption of 3-phosphoinositide-dependent kinase 1 (PDK1) signaling by the anti-tumorigenic and anti-proliferative agent n-alpha-tosyl-l-phenylalanyl chloromethyl ketone.J Biol Chem. 2001 Apr 13;276(15):12466-75.
  • Martin KA, Schalm SS, Romanelli A, Keon KL, Blenis J.Ribosomal S6 kinase 2 inhibition by a potent C-terminal repressor domain is relieved by mitogen-activated protein-extracellular signal-regulated kinase kinase-regulated phosphorylation.J Biol Chem. 2001 Mar 16;276(11):7892-8.
  • Martin KA, Schalm SS, Richardson C, Romanelli A, Keon KL, Blenis J.Regulation of ribosomal S6 kinase 2 by effectors of the phosphoinositide 3-kinase pathway.J Biol Chem. 2001 Mar 16;276(11):7884-91.
  • Shimamura A, Ballif BA, Richards SA, Blenis J.Rsk1 mediates a MEK-MAP kinase cell survival signal.Curr Biol. 2000 Feb 10;10(3):127-35.
  • Lee-Fruman KK, Kuo CJ, Lippincott J, Terada N, Blenis J.Characterization of S6K2, a novel kinase homologous to S6K1.Oncogene. 1999 Sep 9;18(36):5108-14.
  • Richards SA, Fu J, Romanelli A, Shimamura A, Blenis J.Ribosomal S6 kinase 1 (RSK1) activation requires signals dependent on and independent of the MAP kinase ERK.Curr Biol. 1999 Jul 29-Aug 12;9(15):810-20.
  • Romanelli A, Martin KA, Toker A, Blenis J.p70 S6 kinase is regulated by protein kinase Czeta and participates in a phosphoinositide 3-kinase-regulated signalling complex.Mol Cell Biol. 1999 Apr;19(4):2921-8.
  • Juo P, Kuo CJ, Yuan J, Blenis J.Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade.Curr Biol. 1998 Sep 10;8(18):1001-8.
  • Schinkmann K, Blenis J.Cloning and characterization of a human STE20-like protein kinase with unusual cofactor requirements.J Biol Chem. 1997 Nov 7;272(45):28695-703.
  • Grammer TC, Blenis J.Evidence for MEK-independent pathways regulating the prolonged activation of the ERK-MAP kinases.Oncogene. 1997 Apr 10;14(14):1635-42.
  • Juo P, Kuo CJ, Reynolds SE, Konz RF, Raingeaud J, Davis RJ, Biemann HP, Blenis J.Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases.Mol Cell Biol. 1997 Jan;17(1):24-35.
  • Grammer TC, Blenis J.The serine protease inhibitors, tosylphenylalanine chloromethyl ketone and tosyllysine chloromethyl ketone, potently inhibit pp70s6k activation.J Biol Chem. 1996 Sep 27;271(39):23650-2.
  • Nakajima T, Fukamizu A, Takahashi J, Gage FH, Fisher T, Blenis J, Montminy MR.The signal-dependent coactivator CBP is a nuclear target for pp90RSK.Cell. 1996 Aug 9;86(3):465-74.
  • Chou MM, Blenis J.The 70 kDa S6 kinase complexes with and is activated by the Rho family G proteins Cdc42 and Rac1.Cell. 1996 May 17;85(4):573-83.