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Timothy J. Mitchison, PhD

Publications

  • Thurber GM, Yang KS, Reiner T, Kohler RH, Sorger P, Mitchison T, Weissleder R.Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo.Nat Commun. 2013;4:1504.
    23422672
  • Tan ES, Krukenberg KA, Mitchison TJ.Large-scale preparation and characterization of poly(ADP-ribose) and defined length polymers.Anal Biochem. 2012 Sep 15;428(2):126-36.
    22743307
  • Zhang X, Wang W, Bedigian AV, Coughlin ML, Mitchison TJ, Eggert US.Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12485-90.
    22802617
  • Kim S, Peshkin L, Mitchison TJ.Vascular disrupting agent drug classes differ in effects on the cytoskeleton.PLoS ONE. 2012;7(7):e40177.
    22848372
  • Dumont S, Salmon ED, Mitchison TJ.Deformations within moving kinetochores reveal different sites of active and passive force generation.Science. 2012 Jul 20;337(6092):355-8.
    22722252
  • Hu CK, Ozlü N, Coughlin M, Steen JJ, Mitchison TJ.Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.Mol Biol Cell. 2012 Jul;23(14):2702-11.
    22621898
  • Huang HC, Mitchison TJ, Shi J.Stochastic competition between mechanistically independent slippage and death pathways determines cell fate during mitotic arrest.PLoS ONE. 2010;5(12):e15724.
    21203573
  • Wühr M, Tan ES, Parker SK, Detrich HW, Mitchison TJ.A model for cleavage plane determination in early amphibian and fish embryos.Curr Biol. 2010 Nov 23;20(22):2040-5.
    21055946
  • Mitchison TJ.Remaining mysteries of the cytoplasm.Mol Biol Cell. 2010 Nov 15;21(22):3811-2.
    21079024
  • Kueh HY, Brieher WM, Mitchison TJ.Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover.Biophys J. 2010 Oct 6;99(7):2153-62.
    20923649
  • Huang HC, Shi J, Orth JD, Mitchison TJ.Cell death when the SAC is out of commission.Cell Cycle. 2010 Jun 25;9(11).
    20559025
  • Martin DS, Fathi R, Mitchison TJ, Gelles J.FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry.Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5453-8.
    20212149
  • Gatlin JC, Matov A, Danuser G, Mitchison TJ, Salmon ED.Directly probing the mechanical properties of the spindle and its matrix.J Cell Biol. 2010 Feb 22;188(4):481-9.
    20176922
  • Ozlü N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ.Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition.Mol Cell Proteomics. 2010 Feb;9(2):336-50.
    19786723
  • Dumont S, Mitchison TJ.Force and length in the mitotic spindle.Curr Biol. 2009 Sep 15;19(17):R749-61. Review.
    19906577
  • Chang P, Coughlin M, Mitchison TJ.Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly.Mol Biol Cell. 2009 Nov;20(21):4575-85.
    19759176
  • Huang HC, Shi J, Orth JD, Mitchison TJ.Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly.Cancer Cell. 2009 Oct 6;16(4):347-58.
    19800579
  • Tsui M, Xie T, Orth JD, Carpenter AE, Rudnicki S, Kim S, Shamu CE, Mitchison TJ.An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor.PLoS ONE. 2009;4(10):e7339.
    19802393
  • Charras GT, Mitchison TJ, Mahadevan L.Animal cell hydraulics.J Cell Sci. 2009 Sep 15;122(Pt 18):3233-41.
    19690051
  • Kueh HY, Mitchison TJ.Structural plasticity in actin and tubulin polymer dynamics.Science. 2009 Aug 21;325(5943):960-3. Review.
    19696342
  • Maresca TJ, Groen AC, Gatlin JC, Ohi R, Mitchison TJ, Salmon ED.Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity.Curr Biol. 2009 Jul 28;19(14):1210-5.
    19540121
  • Dumont S, Mitchison TJ.Compression regulates mitotic spindle length by a mechanochemical switch at the poles.Curr Biol. 2009 Jul 14;19(13):1086-95.
    19540117
  • Feng Y, Mitchison TJ, Bender A, Young DW, Tallarico JA.Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds.Nat Rev Drug Discov. 2009 Jul;8(7):567-78. Review.
    19568283
  • Niethammer P, Grabher C, Look AT, Mitchison TJ.A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish.Nature. 2009 Jun 18;459(7249):996-9.
    19494811
  • Needleman DJ, Xu Y, Mitchison TJ.Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy.Biophys J. 2009 Jun 17;96(12):5050-9.
    19527665
  • Groen AC,Maresca TJ,Gatlin JC,Salmon ED,Mitchison TJ.Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly.Mol Biol Cell. 2009 Jun;20(11):2766-73.
    19369413
  • Gatlin JC,Matov A,Groen AC,Needleman DJ,Maresca TJ,Danuser G,Mitchison TJ,Salmon ED.Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules.Curr Biol. 2009 Feb 24;19(4):287-96.
    19230671
  • Orth JD,Tang Y,Shi J,Loy CT,Amendt C,Wilm C,Zenke FT,Mitchison TJ.Quantitative live imaging of cancer and normal cells treated with Kinesin-5 inhibitors indicates significant differences in phenotypic responses and cell fate.Mol Cancer Ther. 2008 Nov;7(11):3480-9.
    18974392
  • Maloney KN,Fujita M,Eggert US,Schroeder FC,Field CM,Mitchison TJ,Clardy J.Actin-Aggregating Cucurbitacins from Physocarpus capitatus.J Nat Prod. 2008 Oct 30.
    18959442
  • Wuhr M,Mitchison TJ,Field CM.Mitosis: new roles for myosin-X and actin at the spindle.Curr Biol. 2008 Oct 14;18(19):R912-4.
    18957236
  • Kueh HY,Brieher WM,Mitchison TJ.Dynamic stabilization of actin filaments.Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16531-6.
    18931306
  • Wuhr M,Chen Y,Dumont S,Groen AC,Needleman DJ,Salic A,Mitchison TJ.Evidence for an upper limit to mitotic spindle length.Curr Biol. 2008 Aug 26;18(16):1256-61.
    18718761
  • Kueh HY,Charras GT,Mitchison TJ,Brieher WM.Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers.J Cell Biol. 2008 Jul 28;182(2):341-53.
    18663144
  • Groen AC,Needleman D,Brangwynne C,Gradinaru C,Fowler B,Mazitschek R,Mitchison TJ.A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly.J Cell Sci. 2008 Jul 15;121(Pt 14):2293-300.
    18559893
  • Hu CK, Coughlin M, Field CM, Mitchison TJ.Cell polarization during monopolar cytokinesis.J Cell Biol. 2008 Apr 21;181(2):195-202.
    18411311
  • Niethammer P, Kueh HY, Mitchison TJ.Spatial patterning of metabolism by mitochondria, oxygen, and energy sinks in a model cytoplasm.Curr Biol. 2008 Apr 22;18(8):586-91.
    18406136
  • Mitchison TJ, Charras GT, Mahadevan L.Implications of a poroelastic cytoplasm for the dynamics of animal cell shape.Semin Cell Dev Biol. 2008 Jun;19(3):215-23. Review.
    18395478
  • Salic A, Mitchison TJ.A chemical method for fast and sensitive detection of DNA synthesis in vivo.Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20.
    18272492
  • Galkin VE, Orlova A, Brieher W, Kueh HY, Mitchison TJ, Egelman EH.Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament.J Mol Biol. 2008 Feb 22;376(3):607-13.
    18177666
  • Young DW, Bender A, Hoyt J, McWhinnie E, Chirn GW, Tao CY, Tallarico JA, Labow M, Jenkins JL, Mitchison TJ, Feng Y.Integrating high-content screening and ligand-target prediction to identify mechanism of action.Nat Chem Biol. 2008 Jan;4(1):59-68.
    18066055
  • Irimia D, Charras G, Agrawal N, Mitchison T, Toner M.Polar stimulation and constrained cell migration in microfluidic channels.Lab Chip. 2007 Dec;7(12):1783-90.
    18030401
  • Charras GT, Coughlin M, Mitchison TJ, Mahadevan L.Life and times of a cellular bleb.Biophys J. 2008 Mar 1;94(5):1836-53.
    17921219
  • Burbank KS, Mitchison TJ, Fisher DS.Slide-and-cluster models for spindle assembly.Curr Biol. 2007 Aug 21;17(16):1373-83.
    17702580
  • Vrabioiu AM, Mitchison TJ.Symmetry of septin hourglass and ring structures.J Mol Biol. 2007 Sep 7;372(1):37-49.
    17628594
  • Ohi R, Burbank K, Liu Q, Mitchison TJ.Nonredundant functions of Kinesin-13s during meiotic spindle assembly.Curr Biol. 2007 Jun 5;17(11):953-9.
    17509883
  • Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, Field CM.Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets.PLoS Biol. 2004 Dec;2(12):e379.
    15547975
  • Venkatesh N, Feng Y, DeDecker B, Yacono P, Golan D, Mitchison T, McKeon F.Chemical genetics to identify NFAT inhibitors: potential of targeting calcium mobilization in immunosuppression.Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8969-74.
    15184684
  • Peterson JR, Lokey RS, Mitchison TJ, Kirschner MW.A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions.Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10624-9.
    11553809
  • Degterev A, Lugovskoy A, Cardone M, Mulley B, Wagner G, Mitchison T, Yuan J.Identification of small-molecule inhibitors of interaction between the BH3 domain and Bcl-xL.Nat Cell Biol. 2001 Feb;3(2):173-82.
    11175750
  • Kapoor TM, Mitchison TJ.Allele-specific activators and inhibitors for kinesin.Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9106-11.
    10430903
  • Desai A, Verma S, Mitchison TJ, Walczak CE.Kin I kinesins are microtubule-destabilizing enzymes.Cell. 1999 Jan 8;96(1):69-78.
    9989498
  • Frazier JA, Wong ML, Longtine MS, Pringle JR, Mann M, Mitchison TJ, Field C.Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.J Cell Biol. 1998 Nov 2;143(3):737-49.
    9813094
  • Desai A, Mitchison TJ.Tubulin and FtsZ structures: functional and therapeutic implications.Bioessays. 1998 Jul;20(7):523-7.
    9722999
  • Walczak CE, Vernos I, Mitchison TJ, Karsenti E, Heald R.A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity.Curr Biol. 1998 Jul 30-Aug 13;8(16):903-13.
    9707401
  • Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A.Caged fluorescent probes.Methods Enzymol. 1998;291:63-78.
    9661145
  • Welch MD, Rosenblatt J, Skoble J, Portnoy DA, Mitchison TJ.Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation.Science. 1998 Jul 3;281(5373):105-8.
    9651243
  • Desai A, Maddox PS, Mitchison TJ, Salmon ED.Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.J Cell Biol. 1998 May 4;141(3):703-13.
    9566970
  • Mitchison TJ.Towards a pharmacological genetics.Chem Biol. 1994 Sep;1(1):3-6.
    9383364
  • Desai A, Mitchison TJ.Microtubule polymerization dynamics.Annu Rev Cell Dev Biol. 1997;13:83-117.
    9442869
  • Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.J Cell Biol. 1997 Jul 28;138(2):375-84.
    9230079
  • Rosenblatt J, Agnew BJ, Abe H, Bamburg JR, Mitchison TJ.Xenopus actin depolymerizing factor/cofilin (XAC) is responsible for the turnover of actin filaments in Listeria monocytogenes tails.J Cell Biol. 1997 Mar 24;136(6):1323-32.
    9087446
  • Cramer LP, Siebert M, Mitchison TJ.Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force.J Cell Biol. 1997 Mar 24;136(6):1287-305.
    9087444
  • Walczak CE, Verma S, Mitchison TJ.XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts.J Cell Biol. 1997 Feb 24;136(4):859-70.
    9049251
  • Welch MD, Mallavarapu A, Rosenblatt J, Mitchison TJ.Actin dynamics in vivo.Curr Opin Cell Biol. 1997 Feb;9(1):54-61.
    9013669
  • Welch MD, Iwamatsu A, Mitchison TJ.Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes.Nature. 1997 Jan 16;385(6613):265-9.
    9000076
  • Cramer LP, Mitchison TJ.Investigation of the mechanism of retraction of the cell margin and rearward flow of nodules during mitotic cell rounding.Mol Biol Cell. 1997 Jan;8(1):109-19.
    9017599
  • Field CM, al-Awar O, Rosenblatt J, Wong ML, Alberts B, Mitchison TJ.A purified Drosophila septin complex forms filaments and exhibits GTPase activity.J Cell Biol. 1996 May;133(3):605-16.
    8636235
  • Belmont LD, Mitchison TJ.Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.Cell. 1996 Feb 23;84(4):623-31.
    8598048
  • Mitchison TJ, Cramer LP.Actin-based cell motility and cell locomotion.Cell. 1996 Feb 9;84(3):371-9.
    8608590
  • Walczak CE, Mitchison TJ, Desai A.XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.Cell. 1996 Jan 12;84(1):37-47.
    8548824
  • Cramer LP, Mitchison TJ.Myosin is involved in postmitotic cell spreading.J Cell Biol. 1995 Oct;131(1):179-89.
    7559774
  • Mitchison TJ.Evolution of a dynamic cytoskeleton.Philos Trans R Soc Lond B Biol Sci. 1995 Sep 29;349(1329):299-304.
    8577841
  • Wordeman L, Mitchison TJ.Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.J Cell Biol. 1995 Jan;128(1-2):95-104.
    7822426
  • Hirano T, Mitchison TJ.A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.Cell. 1994 Nov 4;79(3):449-58.
    7954811
  • Theriot JA, Rosenblatt J, Portnoy DA, Goldschmidt-Clermont PJ, Mitchison TJ.Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts.Cell. 1994 Feb 11;76(3):505-17.
    8313471
  • Sawin KE, Mitchison TJ.Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules.Mol Biol Cell. 1994 Feb;5(2):217-26.
    8019007
  • Mitchison TJ.Localization of an exchangeable GTP binding site at the plus end of microtubules.Science. 1993 Aug 20;261(5124):1044-7.
    8102497
  • Cramer L, Mitchison TJ.Moving and stationary actin filaments are involved in spreading of postmitotic PtK2 cells.J Cell Biol. 1993 Aug;122(4):833-43.
    8349733
  • Mitchison TJ, Salmon ED.Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.J Cell Biol. 1992 Nov;119(3):569-82.
    1400593
  • Sawin KE, LeGuellec K, Philippe M, Mitchison TJ.Mitotic spindle organization by a plus-end-directed microtubule motor.Nature. 1992 Oct 8;359(6395):540-3.
    1406972
  • Hyman AA, Middleton K, Centola M, Mitchison TJ, Carbon J.Microtubule-motor activity of a yeast centromere-binding protein complex.Nature. 1992 Oct 8;359(6395):533-6.
    1406970
  • Theriot JA, Mitchison TJ, Tilney LG, Portnoy DA.The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization.Nature. 1992 May 21;357(6375):257-60.
    1589024
  • Mitchison TJ.Self-organization of polymer-motor systems in the cytoskeleton.Philos Trans R Soc Lond B Biol Sci. 1992 Apr 29;336(1276):99-106.
    1351302
  • Sawin KE, Mitchison TJ, Wordeman LG.Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.J Cell Sci. 1992 Feb;101 ( Pt 2):303-13.
    1629247
  • Hirano T, Mitchison TJ.Cell cycle control of higher-order chromatin assembly around naked DNA in vitro.J Cell Biol. 1991 Dec;115(6):1479-89.
    1661728
  • Symons MH, Mitchison TJ.Control of actin polymerization in live and permeabilized fibroblasts.J Cell Biol. 1991 Aug;114(3):503-13.
    1860882
  • Theriot JA, Mitchison TJ.Actin microfilament dynamics in locomoting cells.Nature. 1991 Jul 11;352(6331):126-31.
    2067574
  • Hyman AA, Mitchison TJ.Two different microtubule-based motor activities with opposite polarities in kinetochores.Nature. 1991 May 16;351(6323):206-11.
    2041567
  • Sawin KE, Mitchison TJ.Poleward microtubule flux mitotic spindles assembled in vitro.J Cell Biol. 1991 Mar;112(5):941-54.
    1999464
  • Mitchison TJ.Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.J Cell Biol. 1989 Aug;109(2):637-52.
    2760109
  • Mitchison TJ.Mitosis: basic concepts.Curr Opin Cell Biol. 1989 Feb;1(1):67-74.
    2698210
  • Kirschner MW, Mitchison T.Microtubule dynamics.Nature. 1986 Dec 18-31;324(6098):621.
    3796729
  • Mitchison TJ, Kirschner MW.Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding.J Cell Biol. 1985 Sep;101(3):755-65.
    4030893
  • Mitchison TJ, Kirschner MW.Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation.J Cell Biol. 1985 Sep;101(3):766-77.
    4030894