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Timothy J. Mitchison, PhD


  • Wang Y, Lee YM, Baitsch L, Huang A, Xiang Y, Tong H, Lako A, Von T, Choi C, Lim E, Min J, Li L, Stegmeier F, Schlegel R, Eck MJ, Gray NS, Mitchison TJ, Zhao JJ.MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells.Elife. 2014:e01763.
  • Groen AC, Ngyuen PA, Field CM, Ishihara K, Mitchison TJ.Glycogen-supplemented mitotic cytosol for analyzing Xenopus egg microtubule organization.Methods Enzymol. 2014;540:417-33.
  • Field CM, Nguyen PA, Ishihara K, Groen AC, Mitchison TJ.Xenopus egg cytoplasm with intact actin.Methods Enzymol. 2014;540:399-415.
  • Prentice-Mott HV, Chang CH, Mahadevan L, Mitchison TJ, Irimia D, Shah JV.Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments.Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):21006-11.
  • Coughlin M, Groen AC, Mitchison TJ.Electron microscopy of microtubule cytoskeleton assembly in vitro.Methods Mol Biol. 2014;1117:259-71.
  • Zidovska A, Weitz DA, Mitchison TJ.Micron-scale coherence in interphase chromatin dynamics.Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15555-60.
  • Tang Y, Xie T, Florian S, Moerke N, Shamu C, Benes C, Mitchison TJ.Differential determinants of cancer cell insensitivity to antimitotic drugs discriminated by a one-step cell imaging assay.J Biomol Screen. 2013 Oct;18(9):1062-71.
  • Barkauskaite E, Brassington A, Tan ES, Warwicker J, Dunstan MS, Banos B, Lafite P, Ahel M, Mitchison TJ, Ahel I, Leys D.Visualization of poly(ADP-ribose) bound to PARG reveals inherent balance between exo- and endo-glycohydrolase activities.Nat Commun. 2013;4:2164.
  • Thurber GM, Yang KS, Reiner T, Kohler RH, Sorger P, Mitchison T, Weissleder R.Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo.Nat Commun. 2013;4:1504.
  • Tan ES, Krukenberg KA, Mitchison TJ.Large-scale preparation and characterization of poly(ADP-ribose) and defined length polymers.Anal Biochem. 2012 Sep 15;428(2):126-36.
  • Zhang X, Wang W, Bedigian AV, Coughlin ML, Mitchison TJ, Eggert US.Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12485-90.
  • Kim S, Peshkin L, Mitchison TJ.Vascular disrupting agent drug classes differ in effects on the cytoskeleton.PLoS ONE. 2012;7(7):e40177.
  • Dumont S, Salmon ED, Mitchison TJ.Deformations within moving kinetochores reveal different sites of active and passive force generation.Science. 2012 Jul 20;337(6092):355-8.
  • Hu CK, Ozlü N, Coughlin M, Steen JJ, Mitchison TJ.Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis.Mol Biol Cell. 2012 Jul;23(14):2702-11.
  • Huang HC, Mitchison TJ, Shi J.Stochastic competition between mechanistically independent slippage and death pathways determines cell fate during mitotic arrest.PLoS ONE. 2010;5(12):e15724.
  • Wühr M, Tan ES, Parker SK, Detrich HW, Mitchison TJ.A model for cleavage plane determination in early amphibian and fish embryos.Curr Biol. 2010 Nov 23;20(22):2040-5.
  • Mitchison TJ.Remaining mysteries of the cytoplasm.Mol Biol Cell. 2010 Nov 15;21(22):3811-2.
  • Kueh HY, Brieher WM, Mitchison TJ.Quantitative analysis of actin turnover in Listeria comet tails: evidence for catastrophic filament turnover.Biophys J. 2010 Oct 6;99(7):2153-62.
  • Huang HC, Shi J, Orth JD, Mitchison TJ.Cell death when the SAC is out of commission.Cell Cycle. 2010 Jun 25;9(11).
  • Martin DS, Fathi R, Mitchison TJ, Gelles J.FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry.Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5453-8.
  • Gatlin JC, Matov A, Danuser G, Mitchison TJ, Salmon ED.Directly probing the mechanical properties of the spindle and its matrix.J Cell Biol. 2010 Feb 22;188(4):481-9.
  • Ozlü N, Monigatti F, Renard BY, Field CM, Steen H, Mitchison TJ, Steen JJ.Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition.Mol Cell Proteomics. 2010 Feb;9(2):336-50.
  • Dumont S, Mitchison TJ.Force and length in the mitotic spindle.Curr Biol. 2009 Sep 15;19(17):R749-61. Review.
  • Chang P, Coughlin M, Mitchison TJ.Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly.Mol Biol Cell. 2009 Nov;20(21):4575-85.
  • Huang HC, Shi J, Orth JD, Mitchison TJ.Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly.Cancer Cell. 2009 Oct 6;16(4):347-58.
  • Tsui M, Xie T, Orth JD, Carpenter AE, Rudnicki S, Kim S, Shamu CE, Mitchison TJ.An intermittent live cell imaging screen for siRNA enhancers and suppressors of a kinesin-5 inhibitor.PLoS ONE. 2009;4(10):e7339.
  • Charras GT, Mitchison TJ, Mahadevan L.Animal cell hydraulics.J Cell Sci. 2009 Sep 15;122(Pt 18):3233-41.
  • Kueh HY, Mitchison TJ.Structural plasticity in actin and tubulin polymer dynamics.Science. 2009 Aug 21;325(5943):960-3. Review.
  • Maresca TJ, Groen AC, Gatlin JC, Ohi R, Mitchison TJ, Salmon ED.Spindle assembly in the absence of a RanGTP gradient requires localized CPC activity.Curr Biol. 2009 Jul 28;19(14):1210-5.
  • Dumont S, Mitchison TJ.Compression regulates mitotic spindle length by a mechanochemical switch at the poles.Curr Biol. 2009 Jul 14;19(13):1086-95.
  • Feng Y, Mitchison TJ, Bender A, Young DW, Tallarico JA.Multi-parameter phenotypic profiling: using cellular effects to characterize small-molecule compounds.Nat Rev Drug Discov. 2009 Jul;8(7):567-78. Review.
  • Niethammer P, Grabher C, Look AT, Mitchison TJ.A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish.Nature. 2009 Jun 18;459(7249):996-9.
  • Needleman DJ, Xu Y, Mitchison TJ.Pin-hole array correlation imaging: highly parallel fluorescence correlation spectroscopy.Biophys J. 2009 Jun 17;96(12):5050-9.
  • Groen AC,Maresca TJ,Gatlin JC,Salmon ED,Mitchison TJ.Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly.Mol Biol Cell. 2009 Jun;20(11):2766-73.
  • Gatlin JC,Matov A,Groen AC,Needleman DJ,Maresca TJ,Danuser G,Mitchison TJ,Salmon ED.Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules.Curr Biol. 2009 Feb 24;19(4):287-96.
  • Orth JD,Tang Y,Shi J,Loy CT,Amendt C,Wilm C,Zenke FT,Mitchison TJ.Quantitative live imaging of cancer and normal cells treated with Kinesin-5 inhibitors indicates significant differences in phenotypic responses and cell fate.Mol Cancer Ther. 2008 Nov;7(11):3480-9.
  • Wuhr M,Mitchison TJ,Field CM.Mitosis: new roles for myosin-X and actin at the spindle.Curr Biol. 2008 Oct 14;18(19):R912-4.
  • Maloney KN,Fujita M,Eggert US,Schroeder FC,Field CM,Mitchison TJ,Clardy J.Actin-Aggregating Cucurbitacins from Physocarpus capitatus.J Nat Prod. 2008 Oct 30.
  • Kueh HY,Brieher WM,Mitchison TJ.Dynamic stabilization of actin filaments.Proc Natl Acad Sci U S A. 2008 Oct 28;105(43):16531-6.
  • Wuhr M,Chen Y,Dumont S,Groen AC,Needleman DJ,Salic A,Mitchison TJ.Evidence for an upper limit to mitotic spindle length.Curr Biol. 2008 Aug 26;18(16):1256-61.
  • Kueh HY,Charras GT,Mitchison TJ,Brieher WM.Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers.J Cell Biol. 2008 Jul 28;182(2):341-53.
  • Groen AC,Needleman D,Brangwynne C,Gradinaru C,Fowler B,Mazitschek R,Mitchison TJ.A novel small-molecule inhibitor reveals a possible role of kinesin-5 in anastral spindle-pole assembly.J Cell Sci. 2008 Jul 15;121(Pt 14):2293-300.
  • Hu CK, Coughlin M, Field CM, Mitchison TJ.Cell polarization during monopolar cytokinesis.J Cell Biol. 2008 Apr 21;181(2):195-202.
  • Niethammer P, Kueh HY, Mitchison TJ.Spatial patterning of metabolism by mitochondria, oxygen, and energy sinks in a model cytoplasm.Curr Biol. 2008 Apr 22;18(8):586-91.
  • Mitchison TJ, Charras GT, Mahadevan L.Implications of a poroelastic cytoplasm for the dynamics of animal cell shape.Semin Cell Dev Biol. 2008 Jun;19(3):215-23. Review.
  • Salic A, Mitchison TJ.A chemical method for fast and sensitive detection of DNA synthesis in vivo.Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2415-20.
  • Galkin VE, Orlova A, Brieher W, Kueh HY, Mitchison TJ, Egelman EH.Coronin-1A stabilizes F-actin by bridging adjacent actin protomers and stapling opposite strands of the actin filament.J Mol Biol. 2008 Feb 22;376(3):607-13.
  • Young DW, Bender A, Hoyt J, McWhinnie E, Chirn GW, Tao CY, Tallarico JA, Labow M, Jenkins JL, Mitchison TJ, Feng Y.Integrating high-content screening and ligand-target prediction to identify mechanism of action.Nat Chem Biol. 2008 Jan;4(1):59-68.
  • Irimia D, Charras G, Agrawal N, Mitchison T, Toner M.Polar stimulation and constrained cell migration in microfluidic channels.Lab Chip. 2007 Dec;7(12):1783-90.
  • Charras GT, Coughlin M, Mitchison TJ, Mahadevan L.Life and times of a cellular bleb.Biophys J. 2008 Mar 1;94(5):1836-53.
  • Burbank KS, Mitchison TJ, Fisher DS.Slide-and-cluster models for spindle assembly.Curr Biol. 2007 Aug 21;17(16):1373-83.
  • Vrabioiu AM, Mitchison TJ.Symmetry of septin hourglass and ring structures.J Mol Biol. 2007 Sep 7;372(1):37-49.
  • Ohi R, Burbank K, Liu Q, Mitchison TJ.Nonredundant functions of Kinesin-13s during meiotic spindle assembly.Curr Biol. 2007 Jun 5;17(11):953-9.
  • Eggert US, Kiger AA, Richter C, Perlman ZE, Perrimon N, Mitchison TJ, Field CM.Parallel Chemical Genetic and Genome-Wide RNAi Screens Identify Cytokinesis Inhibitors and Targets.PLoS Biol. 2004 Dec;2(12):e379.
  • Venkatesh N, Feng Y, DeDecker B, Yacono P, Golan D, Mitchison T, McKeon F.Chemical genetics to identify NFAT inhibitors: potential of targeting calcium mobilization in immunosuppression.Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8969-74.
  • Peterson JR, Lokey RS, Mitchison TJ, Kirschner MW.A chemical inhibitor of N-WASP reveals a new mechanism for targeting protein interactions.Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10624-9.
  • Degterev A, Lugovskoy A, Cardone M, Mulley B, Wagner G, Mitchison T, Yuan J.Identification of small-molecule inhibitors of interaction between the BH3 domain and Bcl-xL.Nat Cell Biol. 2001 Feb;3(2):173-82.
  • Kapoor TM, Mitchison TJ.Allele-specific activators and inhibitors for kinesin.Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9106-11.
  • Desai A, Verma S, Mitchison TJ, Walczak CE.Kin I kinesins are microtubule-destabilizing enzymes.Cell. 1999 Jan 8;96(1):69-78.
  • Frazier JA, Wong ML, Longtine MS, Pringle JR, Mann M, Mitchison TJ, Field C.Polymerization of purified yeast septins: evidence that organized filament arrays may not be required for septin function.J Cell Biol. 1998 Nov 2;143(3):737-49.
  • Desai A, Mitchison TJ.Tubulin and FtsZ structures: functional and therapeutic implications.Bioessays. 1998 Jul;20(7):523-7.
  • Walczak CE, Vernos I, Mitchison TJ, Karsenti E, Heald R.A model for the proposed roles of different microtubule-based motor proteins in establishing spindle bipolarity.Curr Biol. 1998 Jul 30-Aug 13;8(16):903-13.
  • Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A.Caged fluorescent probes.Methods Enzymol. 1998;291:63-78.
  • Welch MD, Rosenblatt J, Skoble J, Portnoy DA, Mitchison TJ.Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation.Science. 1998 Jul 3;281(5373):105-8.
  • Desai A, Maddox PS, Mitchison TJ, Salmon ED.Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles.J Cell Biol. 1998 May 4;141(3):703-13.
  • Mitchison TJ.Towards a pharmacological genetics.Chem Biol. 1994 Sep;1(1):3-6.
  • Desai A, Mitchison TJ.Microtubule polymerization dynamics.Annu Rev Cell Dev Biol. 1997;13:83-117.
  • Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ.The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly.J Cell Biol. 1997 Jul 28;138(2):375-84.
  • Cramer LP, Siebert M, Mitchison TJ.Identification of novel graded polarity actin filament bundles in locomoting heart fibroblasts: implications for the generation of motile force.J Cell Biol. 1997 Mar 24;136(6):1287-305.
  • Rosenblatt J, Agnew BJ, Abe H, Bamburg JR, Mitchison TJ.Xenopus actin depolymerizing factor/cofilin (XAC) is responsible for the turnover of actin filaments in Listeria monocytogenes tails.J Cell Biol. 1997 Mar 24;136(6):1323-32.
  • Walczak CE, Verma S, Mitchison TJ.XCTK2: a kinesin-related protein that promotes mitotic spindle assembly in Xenopus laevis egg extracts.J Cell Biol. 1997 Feb 24;136(4):859-70.
  • Welch MD, Mallavarapu A, Rosenblatt J, Mitchison TJ.Actin dynamics in vivo.Curr Opin Cell Biol. 1997 Feb;9(1):54-61.
  • Welch MD, Iwamatsu A, Mitchison TJ.Actin polymerization is induced by Arp2/3 protein complex at the surface of Listeria monocytogenes.Nature. 1997 Jan 16;385(6613):265-9.
  • Cramer LP, Mitchison TJ.Investigation of the mechanism of retraction of the cell margin and rearward flow of nodules during mitotic cell rounding.Mol Biol Cell. 1997 Jan;8(1):109-19.
  • Field CM, al-Awar O, Rosenblatt J, Wong ML, Alberts B, Mitchison TJ.A purified Drosophila septin complex forms filaments and exhibits GTPase activity.J Cell Biol. 1996 May;133(3):605-16.
  • Belmont LD, Mitchison TJ.Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.Cell. 1996 Feb 23;84(4):623-31.
  • Mitchison TJ, Cramer LP.Actin-based cell motility and cell locomotion.Cell. 1996 Feb 9;84(3):371-9.
  • Walczak CE, Mitchison TJ, Desai A.XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.Cell. 1996 Jan 12;84(1):37-47.
  • Cramer LP, Mitchison TJ.Myosin is involved in postmitotic cell spreading.J Cell Biol. 1995 Oct;131(1):179-89.
  • Mitchison TJ.Evolution of a dynamic cytoskeleton.Philos Trans R Soc Lond B Biol Sci. 1995 Sep 29;349(1329):299-304.
  • Wordeman L, Mitchison TJ.Identification and partial characterization of mitotic centromere-associated kinesin, a kinesin-related protein that associates with centromeres during mitosis.J Cell Biol. 1995 Jan;128(1-2):95-104.
  • Hirano T, Mitchison TJ.A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.Cell. 1994 Nov 4;79(3):449-58.
  • Theriot JA, Rosenblatt J, Portnoy DA, Goldschmidt-Clermont PJ, Mitchison TJ.Involvement of profilin in the actin-based motility of L. monocytogenes in cells and in cell-free extracts.Cell. 1994 Feb 11;76(3):505-17.
  • Sawin KE, Mitchison TJ.Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules.Mol Biol Cell. 1994 Feb;5(2):217-26.
  • Mitchison TJ.Localization of an exchangeable GTP binding site at the plus end of microtubules.Science. 1993 Aug 20;261(5124):1044-7.
  • Cramer L, Mitchison TJ.Moving and stationary actin filaments are involved in spreading of postmitotic PtK2 cells.J Cell Biol. 1993 Aug;122(4):833-43.
  • Mitchison TJ, Salmon ED.Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis.J Cell Biol. 1992 Nov;119(3):569-82.
  • Hyman AA, Middleton K, Centola M, Mitchison TJ, Carbon J.Microtubule-motor activity of a yeast centromere-binding protein complex.Nature. 1992 Oct 8;359(6395):533-6.
  • Sawin KE, LeGuellec K, Philippe M, Mitchison TJ.Mitotic spindle organization by a plus-end-directed microtubule motor.Nature. 1992 Oct 8;359(6395):540-3.
  • Theriot JA, Mitchison TJ, Tilney LG, Portnoy DA.The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization.Nature. 1992 May 21;357(6375):257-60.
  • Mitchison TJ.Self-organization of polymer-motor systems in the cytoskeleton.Philos Trans R Soc Lond B Biol Sci. 1992 Apr 29;336(1276):99-106.
  • Sawin KE, Mitchison TJ, Wordeman LG.Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.J Cell Sci. 1992 Feb;101 ( Pt 2):303-13.
  • Hirano T, Mitchison TJ.Cell cycle control of higher-order chromatin assembly around naked DNA in vitro.J Cell Biol. 1991 Dec;115(6):1479-89.
  • Symons MH, Mitchison TJ.Control of actin polymerization in live and permeabilized fibroblasts.J Cell Biol. 1991 Aug;114(3):503-13.
  • Theriot JA, Mitchison TJ.Actin microfilament dynamics in locomoting cells.Nature. 1991 Jul 11;352(6331):126-31.
  • Hyman AA, Mitchison TJ.Two different microtubule-based motor activities with opposite polarities in kinetochores.Nature. 1991 May 16;351(6323):206-11.
  • Sawin KE, Mitchison TJ.Poleward microtubule flux mitotic spindles assembled in vitro.J Cell Biol. 1991 Mar;112(5):941-54.
  • Mitchison TJ.Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.J Cell Biol. 1989 Aug;109(2):637-52.
  • Mitchison TJ.Mitosis: basic concepts.Curr Opin Cell Biol. 1989 Feb;1(1):67-74.
  • Kirschner MW, Mitchison T.Microtubule dynamics.Nature. 1986 Dec 18-31;324(6098):621.
  • Mitchison TJ, Kirschner MW.Properties of the kinetochore in vitro. I. Microtubule nucleation and tubulin binding.J Cell Biol. 1985 Sep;101(3):755-65.
  • Mitchison TJ, Kirschner MW.Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation.J Cell Biol. 1985 Sep;101(3):766-77.