DF/HCC Program Affiliation

Prostate Cancer
Neuro-Oncology
Cancer Immunology

DF/HCC Associations

Director, Vector
Member, Center Scientific Council

Research Abstract

Dr. Mulligan's laboratory is primarily interested in the development of methods for introducing genes into mammalian cells, and the application of those methods in a number of areas of cell biology, developmental biology, virology and medicines. The current research activities of his laboratory are focused in three general areas: (i) the control of hematopoiesis, (ii) manipulation of the immune response via gene transfer, and (iii) the development of new mammalian gene transfer vectors.

Publications

• Mostoslavsky G, Fabian AJ, Rooney S, Alt FW, Mulligan RC.Complete correction of murine Artemis immunodeficiency by lentiviral vector-mediated gene transfer.Proc Natl Acad Sci U S A 2006 Oct 31;103(44):16406-11.
  17062750
• Murphy GJ, Mostoslavsky G, Kotton DN, Mulligan RC.Exogenous control of mammalian gene expression via modulation of translational termination.Nat Med 2006 Sep;12(9):1093-9.
  16892063
• Tam BY, Wei K, Rudge JS, Hoffman J, Holash J, Park SK, Yuan J, Hefner C, Chartier C, Lee JS, Jiang S, Niyak NR, Kuypers FA, Ma L, Sundram U, Wu G, Garcia JA, Schrier SL, Maher JJ, Johnson RS, Yancopoulos GD, Mulligan RC, Kuo CJ.VEGF modulates erythropoies
  16799557
• Yen L, Magnier M, Weissleder R, Stockwell BR, Mulligan RC.Identification of inhibitors of ribozyme self-cleavage in mammalian cells via high-throughput screening of chemical libraries.RNA 2006 May;12(5):797-806.
  16556935
• Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and
  16462491
• Balazs AB, Fabian AJ, Esmon CT, Mulligan RC.Endothelial protein C receptor (CD201) explicitly identifies hematopoietic stem cells in murine bone marrow.Blood 2006 Mar 15;107(6):2317-21.
  16304059
• Gussoni E, Soneoka Y, Strickland CD, Buzney EA, Khan MK, Flint AF, Kunkel LM, Mulligan RC.Dystrophin expression in the mdx mouse restored by stem cell transplantation.Nature 1999 Sep 23;401(6751):390-4.
  10517639
• Goodell MA, Rosenzweig M, Kim H, Marks DF, DeMaria M, Paradis G, Grupp SA, Sieff CA, Mulligan RC, Johnson RP.Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species.Nat Med 1
  9396603
• Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC.Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo.J Exp Med 1996 Apr 1;183(4):1797-806.
  8666936
• Szentirmai O, Baker CH, Bullain SS, Lin N, Takahashi M, Folkman J, Mulligan RC, Carter BS.Successful inhibition of intracranial human glioblastoma multiforme xenograft growth via systemic adenoviral delivery of soluble endostatin and soluble vascular endo
  18447716
• Austin KM, Gupta ML, Coats SA, Tulpule A, Mostoslavsky G, Balazs AB, Mulligan RC, Daley G, Pellman D, Shimamura A.Mitotic spindle destabilization and genomic instability in Shwachman-Diamond syndrome.J Clin Invest 2008 Apr;118(4):1511-8.
  18324336
• Chan RK, Garfein E, Gigante PR, Liu P, Agha RA, Mulligan R, Orgill DP.Side population hematopoietic stem cells promote wound healing in diabetic mice.Plast Reconstr Surg 2007 Aug;120(2):407-11; discussion 412-3.
  17632341
• Butler MO, Lee JS, Ansén S, Neuberg D, Hodi FS, Murray AP, Drury L, Berezovskaya A, Mulligan RC, Nadler LM, Hirano N.Long-Lived Antitumor CD8+ Lymphocytes for Adoptive Therapy Generated Using an Artificial Antigen-Presenting Cell.Clin Cancer Res 2007 Mar
  17363542
• Kaipainen A, Kieran MW, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, Mulligan R, Folkman J, Panigrahy D.PPARalpha Deficiency in Inflammatory Cells Suppresses Tumor Growth.PLoS ONE 2007;2:e260.
  17327920
• Hägerkvist R, Mokhtari D, Lindholm C, Farnebo F, Mostoslavsky G, Mulligan RC, Welsh N, Welsh M.Consequences of Shb and c-Abl interactions for cell death in response to various stress stimuli.Exp Cell Res 2007 Jan 15;313(2):284-91.
  17112510
• Yan CT, Kaushal D, Murphy M, Zhang Y, Datta A, Chen C, Monroe B, Mostoslavsky G, Coakley K, Gao Y, Mills KD, Fazeli AP, Tepsuporn S, Hall G, Mulligan R, Fox E, Bronson R, De Girolami U, Lee C, Alt FW.XRCC4 suppresses medulloblastomas with recurrent transl
  16670198
• Mostoslavsky R, Chua KF, Lombard DB, Pang WW, Fischer MR, Gellon L, Liu P, Mostoslavsky G, Franco S, Murphy MM, Mills KD, Patel P, Hsu JT, Hong AL, Ford E, Cheng HL, Kennedy C, Nunez N, Bronson R, Frendewey D, Auerbach W, Valenzuela D, Karow M, Hottiger M
  16439206
• Fernandes JR, Duvivier-Kali VF, Keegan M, Hollister-Lock J, Omer A, Su S, Bonner-Weir S, Feng S, Lee JS, Mulligan RC, Weir GC.Transplantation of islets transduced with CTLA4-Ig and TGFbeta using adenovirus and lentivirus vectors.Transpl Immunol 2004 Nov;1
  15381202
• Perron MJ, Stremlau M, Song B, Ulm W, Mulligan RC, Sodroski J.TRIM5alpha mediates the postentry block to N-tropic murine leukemia viruses in human cells.Proc Natl Acad Sci U S A 2004 Aug 10;101(32):11827-32.
  15280539
• Soiffer R, Hodi FS, Haluska F, Jung K, Gillessen S, Singer S, Tanabe K, Duda R, Mentzer S, Jaklitsch M, Bueno R, Clift S, Hardy S, Neuberg D, Mulligan R, Webb I, Mihm M, Dranoff G.Vaccination with irradiated, autologous melanoma cells engineered to secret
  12947071
• Robert C, Klein C, Cheng G, Kogan A, Mulligan RC, von Andrian UH, Kupper TS.Gene therapy to target dendritic cells from blood to lymph nodes.Gene Ther 2003 Aug;10(17):1479-86.
  12900763
• Salgia R, Lynch T, Skarin A, Lucca J, Lynch C, Jung K, Hodi FS, Jaklitsch M, Mentzer S, Swanson S, Lukanich J, Bueno R, Wain J, Mathisen D, Wright C, Fidias P, Donahue D, Clift S, Hardy S, Neuberg D, Mulligan R, Webb I, Sugarbaker D, Mihm M, Dranoff G.Vac
  12586798
• Klein C, Nguyen D, Liu CH, Mizoguchi A, Bhan AK, Miki H, Takenawa T, Rosen FS, Alt FW, Mulligan RC, Snapper SB.Gene therapy for Wiskott-Aldrich syndrome: rescue of T-cell signaling and amelioration of colitis upon transplantation of retrovirally transduce
  12433691
• Heinz M, Huang CA, Emery DW, Giovino MA, LeGuern A, Kurilla-Mahon B, Theodore P, Arn JS, Sykes M, Mulligan R, Down JD, Sachs DH, Goodell MA.Use of CD9 expression to enrich for porcine hematopoietic progenitors.Exp Hematol 2002 Jul;30(7):809-15.
  12135680
• Niederman TM, Ghogawala Z, Carter BS, Tompkins HS, Russell MM, Mulligan RC.Antitumor activity of cytotoxic T lymphocytes engineered to target vascular endothelial growth factor receptors.Proc Natl Acad Sci U S A 2002 May 14;99(10):7009-14.
  11997459
• Snapper SB, Takeshima F, Antón I, Liu CH, Thomas SM, Nguyen D, Dudley D, Fraser H, Purich D, Lopez-Ilasaca M, Klein C, Davidson L, Bronson R, Mulligan RC, Southwick F, Geha R, Goldberg MB, Rosen FS, Hartwig JH, Alt FW.N-WASP deficiency reveals distinct pa
  11584271
• Rebel VI, Tanaka M, Lee JS, Hartnett S, Pulsipher M, Nathan DG, Mulligan RC, Sieff CA.One-day ex vivo culture allows effective gene transfer into human nonobese diabetic/severe combined immune-deficient repopulating cells using high-titer vesicular stomat
  10090930
• Pulsipher M, Kupfer GM, Naf D, Suliman A, Lee JS, Jakobs P, Grompe M, Joenje H, Sieff C, Guinan E, Mulligan R, D'Andrea AD.Subtyping analysis of Fanconi anemia by immunoblotting and retroviral gene transfer.Mol Med 1998 Jul;4(7):468-79.
  9713825
• Lantz CS, Boesiger J, Song CH, Mach N, Kobayashi T, Mulligan RC, Nawa Y, Dranoff G, Galli SJ.Role for interleukin-3 in mast-cell and basophil development and in immunity to parasites.Nature 1998 Mar 5;392(6671):90-3.
  9510253
• McArthur JG, Mulligan RC.Induction of protective anti-tumor immunity by gene-modified dendritic cells.J Immunother 1998 Jan;21(1):41-7.
  9456435
• Mach N, Lantz CS, Galli SJ, Reznikoff G, Mihm M, Small C, Granstein R, Beissert S, Sadelain M, Mulligan RC, Dranoff G.Involvement of interleukin-3 in delayed-type hypersensitivity.Blood 1998 Feb 1;91(3):778-83.
  9446636
• Lindemann D, Patriquin E, Feng S, Mulligan RC.Versatile retrovirus vector systems for regulated gene expression in vitro and in vivo.Mol Med 1997 Jul;3(7):466-76.
  9260158
• Lieschke GJ, Rao PK, Gately MK, Mulligan RC.Bioactive murine and human interleukin-12 fusion proteins which retain antitumor activity in vivo.Nat Biotechnol 1997 Jan;15(1):35-40.
  9035103
• Ory DS, Neugeboren BA, Mulligan RC.A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes.Proc Natl Acad Sci U S A 1996 Oct 15;93(21):11400-6.
  8876147
• Büeler H, Mulligan RC.Induction of antigen-specific tumor immunity by genetic and cellular vaccines against MAGE: enhanced tumor protection by coexpression of granulocyte-macrophage colony-stimulating factor and B7-1.Mol Med 1996 Sep;2(5):545-55.
  8898371
• Rivière I, Brose K, Mulligan RC.Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells.Proc Natl Acad Sci U S A 1995 Jul 18;92(15):6733-7.
  7624312
• Carmeliet P, Schoonjans L, Kieckens L, Ream B, Degen J, Bronson R, De Vos R, van den Oord JJ, Collen D, Mulligan RC.Physiological consequences of loss of plasminogen activator gene function in mice.Nature 1994 Mar 31;368(6470):419-24.
  8133887
• Sanda MG, Ayyagari SR, Jaffee EM, Epstein JI, Clift SL, Cohen LK, Dranoff G, Pardoll DM, Mulligan RC, Simons JW.Demonstration of a rational strategy for human prostate cancer gene therapy.J Urol 1994 Mar;151(3):622-8.
  8308972
• Carmeliet P, Stassen JM, Schoonjans L, Ream B, van den Oord JJ, De Mol M, Mulligan RC, Collen D.Plasminogen activator inhibitor-1 gene-deficient mice. II. Effects on hemostasis, thrombosis, and thrombolysis.J Clin Invest 1993 Dec;92(6):2756-60.
  8254029
• Mulligan RC.The basic science of gene therapy.Science 1993 May 14;260(5110):926-32.
  8493530
• Dranoff G, Jaffee E, Lazenby A, Golumbek P, Levitsky H, Brose K, Jackson V, Hamada H, Pardoll D, Mulligan RC.Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific,
  8097319
• Spain LM, Mulligan RC.Purification and characterization of retrovirally transduced hematopoietic stem cells.Proc Natl Acad Sci U S A 1992 May 1;89(9):3790-4.
  1570297
• Wilson JM, Danos O, Grossman M, Raulet DH, Mulligan RC.Expression of human adenosine deaminase in mice reconstituted with retrovirus-transduced hematopoietic stem cells.Proc Natl Acad Sci U S A 1990 Jan;87(1):439-43.
  2296599
• Wilson JM, Birinyi LK, Salomon RN, Libby P, Callow AD, Mulligan RC.Implantation of vascular grafts lined with genetically modified endothelial cells.Science 1989 Jun 16;244(4910):1344-6.
  2734614
• Hartman SC, Mulligan RC.Two dominant-acting selectable markers for gene transfer studies in mammalian cells.Proc Natl Acad Sci U S A 1988 Nov;85(21):8047-51.
  3054879
• Danos O, Mulligan RC.Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.Proc Natl Acad Sci U S A 1988 Sep;85(17):6460-4.
  3413107
• Cone RD, Weber-Benarous A, Baorto D, Mulligan RC.Regulated expression of a complete human beta-globin gene encoded by a transmissible retrovirus vector.Mol Cell Biol 1987 Feb;7(2):887-97.
  3029570
• Lemischka IR, Raulet DH, Mulligan RC.Developmental potential and dynamic behavior of hematopoietic stem cells.Cell 1986 Jun 20;45(6):917-27.
  2871944
• Williams DA, Lemischka IR, Nathan DG, Mulligan RC.Introduction of new genetic material into pluripotent haematopoietic stem cells of the mouse.Nature 1984 Aug 9-15;310(5977):476-80.
  6087158