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Stephen Buratowski, PhD

Publications

  • Hazelbaker DZ, Buratowski S.Transcription: base j blocks the way.Curr Biol. 2012 Nov 20;22(22):R960-2.
    23174300
  • Sikorski TW, Joo YJ, Ficarro SB, Askenazi M, Buratowski S, Marto JA.Proteomic Analysis Demonstrates Activator- and Chromatin-specific Recruitment to Promoters.J Biol Chem. 2012 Oct 12;287(42):35397-408.
    22902623
  • van Werven FJ, Neuert G, Hendrick N, Lardenois A, Buratowski S, van Oudenaarden A, Primig M, Amon A.Transcription of two long noncoding RNAs mediates mating-type control of gametogenesis in budding yeast.Cell. 2012 Sep 14;150(6):1170-81.
    22959267
  • Kim T, Xu Z, Clauder-M√ľnster S, Steinmetz LM, Buratowski S.Set3 HDAC mediates effects of overlapping noncoding transcription on gene induction kinetics.Cell. 2012 Sep 14;150(6):1158-69.
    22959268
  • Weiner A, Chen HV, Liu CL, Rahat A, Klien A, Soares L, Gudipati M, Pfeffner J, Regev A, Buratowski S, Pleiss JA, Friedman N, Rando OJ.Systematic dissection of roles for chromatin regulators in a yeast stress response.PLoS Biol. 2012;10(7):e1001369.
    22912562
  • Radman-Livaja M, Quan TK, Valenzuela L, Armstrong JA, van Welsem T, Kim T, Lee LJ, Buratowski S, van Leeuwen F, Rando OJ, Hartzog GA.A key role for Chd1 in histone H3 dynamics at the 3' ends of long genes in yeast.PLoS Genet. 2012 Jul;8(7):e1002811.
    22807688
  • Dermody JL, Buratowski S.Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment.J Biol Chem. 2010 Oct 29;285(44):33671-9.
    20732871
  • Lunde BM, Reichow SL, Kim M, Suh H, Leeper TC, Yang F, Mutschler H, Buratowski S, Meinhart A, Varani G.Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain.Nat Struct Mol Biol. 2010 Oct;17(10):1195-201.
    20818393
  • Kim TS, Liu CL, Yassour M, Holik J, Friedman N, Buratowski S, Rando OJ.RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast.Genome Biol. 2010;11(7):R75.
    20637075
  • Buratowski S.Progression through the RNA polymerase II CTD cycle.Mol Cell. 2009 Nov 25;36(4):541-6. Review.
    19941815
  • Johnson A, Li G, Sikorski TW, Buratowski S, Woodcock CL, Moazed D.Reconstitution of heterochromatin-dependent transcriptional gene silencing.Mol Cell. 2009 Sep 24;35(6):769-81.
    19782027
  • Kim M, Suh H, Cho EJ, Buratowski S.Phosphorylation of the yeast Rpb1 C-terminal domain at serines 2, 5, and 7.J Biol Chem. 2009 Sep 25;284(39):26421-6.
    19679665
  • Sikorski TW,Buratowski S.The basal initiation machinery: beyond the general transcription factors.Curr Opin Cell Biol. 2009 Jun;21(3):344-51. Review.
    19411170
  • Kim T,Buratowski S.Dimethylation of H3K4 by Set1 recruits the Set3 histone deacetylase complex to 5' transcribed regions.Cell. 2009 Apr 17;137(2):259-72.
    19379692
  • Ahn SH,Keogh MC,Buratowski S.Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II.EMBO J. 2009 Feb 4;28(3):205-12.
    19131970
  • Buratowski S.Transcription. Gene expression--where to start?.Science. 2008 Dec 19;322(5909):1804-5.
    19095933
  • Dermody JL,Dreyfuss JM,Villen J,Ogundipe B,Gygi SP,Park PJ,Ponticelli AS,Moore CL,Buratowski S,Bucheli ME.Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation.PLoS ONE. 2008;3(9):e3273.
    18818768
  • Vasiljeva L,Kim M,Mutschler H,Buratowski S,Meinhart A.The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain.Nat Struct Mol Biol. 2008 Aug;15(8):795-804.
    18660819
  • Vasiljeva L, Kim M, Terzi N, Soares LM, Buratowski S.Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin.Mol Cell. 2008 Feb 15;29(3):313-23.
    18280237
  • Runner VM, Podolny V, Buratowski S.The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors.Mol Cell Biol. 2008 Mar;28(6):1883-91.
    18195044
  • Deka P, Bucheli ME, Moore C, Buratowski S, Varani G.Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals.J Mol Biol. 2008 Jan 4;375(1):136-50.
    18022637
  • Bucheli ME, He X, Kaplan CD, Moore CL, Buratowski S.Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI.RNA. 2007 Oct;13(10):1756-64.
    17684230
  • Huarte M, Lan F, Kim T, Vaughn MW, Zaratiegui M, Martienssen RA, Buratowski S, Shi Y.The fission yeast Jmj2 reverses histone H3 Lysine 4 trimethylation.J Biol Chem. 2007 Jul 27;282(30):21662-70.
    17550896
  • Kim T, Buratowski S.Two Saccharomyces cerevisiae JmjC domain proteins demethylate histone H3 Lys36 in transcribed regions to promote elongation.J Biol Chem. 2007 Jul 20;282(29):20827-35.
    17525156
  • Keogh MC, Mennella TA, Sawa C, Berthelet S, Krogan NJ, Wolek A, Podolny V, Carpenter LR, Greenblatt JF, Baetz K, Buratowski S.The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4.Genes Dev. 2006 Mar 15;20(6):660-5.
    16543219
  • Vasiljeva L, Buratowski S.Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts.Mol Cell. 2006 Jan 20;21(2):239-48.
    16427013
  • Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J.gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair.Mol Cell. 2005 Dec 9;20(5):801-9.
    16310392
  • Keogh MC, Kim JA, Downey M, Fillingham J, Chowdhury D, Harrison JC, Onishi M, Datta N, Galicia S, Emili A, Lieberman J, Shen X, Buratowski S, Haber JE, Durocher D, Greenblatt JF, Krogan NJ.A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery.Nature. 2006 Jan 26;439(7075):497-501.
    16299494
  • Keogh MC, Kurdistani SK, Morris SA, Ahn SH, Podolny V, Collins SR, Schuldiner M, Chin K, Punna T, Thompson NJ, Boone C, Emili A, Weissman JS, Hughes TR, Strahl BD, Grunstein M, Greenblatt JF, Buratowski S, Krogan NJ.Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex.Cell. 2005 Nov 18;123(4):593-605.
    16286008
  • Liu CL, Kaplan T, Kim M, Buratowski S, Schreiber SL, Friedman N, Rando OJ.Single-nucleosome mapping of histone modifications in S. cerevisiae.PLoS Biol. 2005 Oct;3(10):e328.
    16122352
  • Buratowski S, Moazed D.Gene regulation: expression and silencing coupled.Nature. 2005 Jun 30;435(7046):1174-5.
    15988510
  • Buratowski S.Connections between mRNA 3' end processing and transcription termination.Curr Opin Cell Biol. 2005 Jun;17(3):257-61.
    15901494
  • Bucheli ME, Buratowski S.Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II.EMBO J. 2005 Jun 15;24(12):2150-60.
    15902270
  • Krogan NJ, Lam MH, Fillingham J, Keogh MC, Gebbia M, Li J, Datta N, Cagney G, Buratowski S, Emili A, Greenblatt JF.Proteasome involvement in the repair of DNA double-strand breaks.Mol Cell. 2004 Dec 22;16(6):1027-34.
    15610744
  • Kim M, Krogan NJ, Vasiljeva L, Rando OJ, Nedea E, Greenblatt JF, Buratowski S.The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II.Nature. 2004 Nov 25;432(7016):517-22.
    15565157
  • Auty R, Steen H, Myers LC, Persinger J, Bartholomew B, Gygi SP, Buratowski S.Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function.J Biol Chem. 2004 Nov 26;279(48):49973-81.
    15448131
  • Krogan NJ, Baetz K, Keogh MC, Datta N, Sawa C, Kwok TC, Thompson NJ, Davey MG, Pootoolal J, Hughes TR, Emili A, Buratowski S, Hieter P, Greenblatt JF.Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4.Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13513-8.
    15353583
  • Sawa C, Nedea E, Krogan N, Wada T, Handa H, Greenblatt J, Buratowski S.Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2.Mol Cell Biol. 2004 Jun;24(11):4734-42.
    15143168
  • Matangkasombut O, Auty R, Buratowski S.Structure and function of the TFIID complex.Adv Protein Chem. 2004;67:67-92.
    14969724
  • Keogh MC, Buratowski S.Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in Saccharomyces cerevisiae.Methods Mol Biol. 2004;257:1-16.
    14769992
  • Kim M, Ahn SH, Krogan NJ, Greenblatt JF, Buratowski S.Transitions in RNA polymerase II elongation complexes at the 3' ends of genes.EMBO J. 2004 Jan 28;23(2):354-64.
    14739930
  • Ahn SH, Kim M, Buratowski S.Phosphorylation of serine 2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing.Mol Cell. 2004 Jan 16;13(1):67-76.
    14731395
  • Krogan NJ, Keogh MC, Datta N, Sawa C, Ryan OW, Ding H, Haw RA, Pootoolal J, Tong A, Canadien V, Richards DP, Wu X, Emili A, Hughes TR, Buratowski S, Greenblatt JF.A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.Mol Cell. 2003 Dec;12(6):1565-76.
    14690608
  • Keogh MC, Podolny V, Buratowski S.Bur1 kinase is required for efficient transcription elongation by RNA polymerase II.Mol Cell Biol. 2003 Oct;23(19):7005-18.
    12972617
  • Buratowski S.The CTD code.Nat Struct Biol. 2003 Sep;10(9):679-80.
    12942140
  • Nedea E, He X, Kim M, Pootoolal J, Zhong G, Canadien V, Hughes T, Buratowski S, Moore CL, Greenblatt J.Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends.J Biol Chem. 2003 Aug 29;278(35):33000-10.
    12819204
  • Krogan NJ, Kim M, Tong A, Golshani A, Cagney G, Canadien V, Richards DP, Beattie BK, Emili A, Boone C, Shilatifard A, Buratowski S, Greenblatt J.Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II.Mol Cell Biol. 2003 Jun;23(12):4207-18.
    12773564
  • Matangkasombut O, Buratowski S.Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation.Mol Cell. 2003 Feb;11(2):353-63.
    12620224
  • Takagi T, Walker AK, Sawa C, Diehn F, Takase Y, Blackwell TK, Buratowski S.The Caenorhabditis elegans mRNA 5'-capping enzyme. In vitro and in vivo characterization.J Biol Chem. 2003 Apr 18;278(16):14174-84.
    12576476
  • Takagi T, Cho EJ, Janoo RT, Polodny V, Takase Y, Keogh MC, Woo SA, Fresco-Cohen LD, Hoffman CS, Buratowski S.Divergent subunit interactions among fungal mRNA 5'-capping machineries.Eukaryot Cell. 2002 Jun;1(3):448-57.
    12455993
  • Buratowski RM, Downs J, Buratowski S.Interdependent interactions between TFIIB, TATA binding protein, and DNA.Mol Cell Biol. 2002 Dec;22(24):8735-43.
    12446790
  • Krogan NJ, Kim M, Ahn SH, Zhong G, Kobor MS, Cagney G, Emili A, Shilatifard A, Buratowski S, Greenblatt JF.RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach.Mol Cell Biol. 2002 Oct;22(20):6979-92.
    12242279
  • Keogh MC, Cho EJ, Podolny V, Buratowski S.Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with differential sensitivities to T-loop phosphorylation.Mol Cell Biol. 2002 Mar;22(5):1288-97.
    11839796
  • Cho EJ, Kobor MS, Kim M, Greenblatt J, Buratowski S.Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain.Genes Dev. 2001 Dec 15;15(24):3319-29.
    11751637
  • Selleck W, Howley R, Fang Q, Podolny V, Fried MG, Buratowski S, Tan S.A histone fold TAF octamer within the yeast TFIID transcriptional coactivator.Nat Struct Biol. 2001 Aug;8(8):695-700.
    11473260
  • Takagi T, Buratowski S.A Plasmodium falciparum protein related to fungal RNA 5'-triphosphatases.Mol Biochem Parasitol. 2001 May;114(2):239-44.
    11378203
  • Takase Y, Takagi T, Komarnitsky PB, Buratowski S.The essential interaction between yeast mRNA capping enzyme subunits is not required for triphosphatase function in vivo.Mol Cell Biol. 2000 Dec;20(24):9307-16.
    11094081
  • Komarnitsky P, Cho EJ, Buratowski S.Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription.Genes Dev. 2000 Oct 1;14(19):2452-60.
    11018013
  • Buratowski S.Snapshots of RNA polymerase II transcription initiation.Curr Opin Cell Biol. 2000 Jun;12(3):320-5.
    10801469
  • Matangkasombut O, Buratowski RM, Swilling NW, Buratowski S.Bromodomain factor 1 corresponds to a missing piece of yeast TFIID.Genes Dev. 2000 Apr 15;14(8):951-62.
    10783167
  • Rodriguez CR, Cho EJ, Keogh MC, Moore CL, Greenleaf AL, Buratowski S.Kin28, the TFIIH-associated carboxy-terminal domain kinase, facilitates the recruitment of mRNA processing machinery to RNA polymerase II.Mol Cell Biol. 2000 Jan;20(1):104-12.
    10594013
  • Morehouse H, Buratowski RM, Silver PA, Buratowski S.The importin/karyopherin Kap114 mediates the nuclear import of TATA-binding protein.Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12542-7.
    10535958
  • Komarnitsky PB, Michel B, Buratowski S.TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo.Genes Dev. 1999 Oct 1;13(19):2484-9.
    10521393
  • Deshpande T, Takagi T, Hao L, Buratowski S, Charbonneau H.Human PIR1 of the protein-tyrosine phosphatase superfamily has RNA 5'-triphosphatase and diphosphatase activities.J Biol Chem. 1999 Jun 4;274(23):16590-4.
    10347225
  • Rodriguez CR, Takagi T, Cho EJ, Buratowski S.A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme.Nucleic Acids Res. 1999 May 15;27(10):2181-8.
    10219091
  • Michel B, Komarnitsky P, Buratowski S.Histone-like TAFs are essential for transcription in vivo.Mol Cell. 1998 Nov;2(5):663-73.
    9844638
  • Cho EJ, Rodriguez CR, Takagi T, Buratowski S.Allosteric interactions between capping enzyme subunits and the RNA polymerase II carboxy-terminal domain.Genes Dev. 1998 Nov 15;12(22):3482-7.
    9832501
  • Cho EJ, Takagi T, Moore CR, Buratowski S.mRNA capping enzyme is recruited to the transcription complex by phosphorylation of the RNA polymerase II carboxy-terminal domain.Genes Dev. 1997 Dec 15;11(24):3319-26.
    9407025
  • Kuldell NH, Buratowski S.Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions.Mol Cell Biol. 1997 Sep;17(9):5288-98.
    9271406
  • Takagi T, Moore CR, Diehn F, Buratowski S.An RNA 5'-triphosphatase related to the protein tyrosine phosphatases.Cell. 1997 Jun 13;89(6):867-73.
    9200605
  • Moqtaderi Z, Yale JD, Struhl K, Buratowski S.Yeast homologues of higher eukaryotic TFIID subunits.Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14654-8.
    8962109
  • Fresco LD, Buratowski S.Conditional mutants of the yeast mRNA capping enzyme show that the cap enhances, but is not required for, mRNA splicing.RNA. 1996 Jun;2(6):584-96.
    8718687
  • Matsui P, DePaulo J, Buratowski S.An interaction between the Tfb1 and Ssl1 subunits of yeast TFIIH correlates with DNA repair activity.Nucleic Acids Res. 1995 Mar 11;23(5):767-72.
    7708491
  • Fresco LD, Buratowski S.Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases.Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6624-8.
    8022828
  • Buratowski S, Zhou H.Functional domains of transcription factor TFIIB.Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5633-7.
    8516312
  • Buratowski S, Zhou H.A suppressor of TBP mutations encodes an RNA polymerase III transcription factor with homology to TFIIB.Cell. 1992 Oct 16;71(2):221-30.
    1423590