DF/HCC Program Affiliation

Breast Cancer

Research Abstract

A major research activity is managing the Brigham and Women’s Hospital breast tumor frozen tissue bank. Since its establishment 4 years ago, the breast tumor bank has become an extensively utilized and invaluable tissue resource to scientists in our affiliated institutions for genomic research, gene validation, somatic mutation screening, and clinical outcomes research. My own research includes gene expression array analysis of human breast tumors to study cancer heterogeneity and taxonomy and to investigate the use of gene expression patterns to predict cancer stage or prognosis. I am particularly interested in the newly described basal-like breast tumor subset identified by expression array analysis. We have performed SNP array-based genome-wide loss-of-heterozygosity (LOH) analysis on the same tumor cohort and have found unique genomic alterations in certain subclasses of breast cancer. Future directions will include further LOH mapping of these unique regions and testing of candidate genes for mutations in the involved tumors. We will also seek to validate expression of specific gene markers for identification of the basal-like tumors by immunohistochemistry on tissue microarrays.

Publications

• Shipitsin M, Campbell LL, Argani P, Weremowicz S, Bloushtain-Qimron N, Yao J, Nikolskaya T, Serebryiskaya T, Beroukhim R, Hu M, Halushka MK, Sukumar S, Parker LM, Anderson KS, Harris LN, Garber JE, Richardson AL, Schnitt SJ, Nikolsky Y, Gelman RS, Polyak
  17349583
• Maher EA, Brennan C, Wen PY, Durso L, Ligon KL, Richardson A, Khatry D, Feng B, Sinha R, Louis DN, Quackenbush J, Black PM, Chin L, DePinho RA.Marked genomic differences characterize primary and secondary glioblastoma subtypes and identify two distinct mo
  17114236
• Wang ZC, Buraimoh A, Iglehart JD, Richardson AL.Genome-Wide Analysis for Loss of Heterozygosity in Primary and Recurrent Phyllodes Tumor and Fibroadenoma of Breast using Single Nucleotide Polymorphism Arrays.Breast Cancer Res Treat 2006 Apr 27.
  16642273
• Richardson AL, Wang ZC, De Nicolo A, Lu X, Brown M, Miron A, Liao X, Iglehart JD, Livingston DM, Ganesan S.X chromosomal abnormalities in basal-like human breast cancer.Cancer Cell 2006 Feb;9(2):121-32.
  16473279
• Golshan M, Kuten A, William J, Richardson A, Modarressi A, Matulonis U.Metaplastic carcinoma of the breast with neuroglial differentiation.Breast 2005 Oct 24.
  16253505
• Kuperwasser C, Chavarria T, Wu M, Magrane G, Gray JW, Carey L, Richardson A, Weinberg RA.Reconstruction of functionally normal and malignant human breast tissues in mice.Proc Natl Acad Sci U S A 2004 Mar 29.
  15051869
• Wang ZC, Lin M, Wei LJ, Li C, Miron A, Lodeiro G, Harris L, Ramaswamy S, Tanenbaum DM, Meyerson M, Iglehart JD, Richardson A.Loss of heterozygosity and its correlation with expression profiles in subclasses of invasive breast cancers.Cancer Res 2004 Jan 1
  14729609
• Krop I, Maguire P, Lahti-Domenici J, Lodeiro G, Richardson A, Johannsdottir HK, Nevanlinna H, Borg A, Gelman R, Barkardottir RB, Lindblom A, Polyak K.Lack of HIN-1 methylation in BRCA1-linked and "BRCA1-like" breast tumors.Cancer Res 2003 May 1;
  12727813
• Signoretti S, Di Marcotullio L, Richardson A, Ramaswamy S, Isaac B, Rue M, Monti F, Loda M, Pagano M.Oncogenic role of the ubiquitin ligase subunit Skp2 in human breast cancer.J Clin Invest 2002 Sep;110(5):633-41.
  12208864
• Hu M, Yao J, Carroll DK, Weremowicz S, Chen H, Carrasco D, Richardson A, Violette S, Nikolskaya T, Nikolsky Y, Bauerlein EL, Hahn WC, Gelman RS, Allred C, Bissell MJ, Schnitt S, Polyak K.Regulation of in situ to invasive breast carcinoma transition.Cancer
  18455123
• Yang XH, Richardson AL, Torres-Arzayus MI, Zhou P, Sharma C, Kazarov AR, Andzelm MM, Strominger JL, Brown M, Hemler ME.CD151 accelerates breast cancer by regulating alpha 6 integrin function, signaling, and molecular organization.Cancer Res 2008 May 1;68(
  18451146
• Lu X, Lu X, Wang ZC, Iglehart JD, Zhang X, Richardson AL.Predicting features of breast cancer with gene expression patterns.Breast Cancer Res Treat 2008 Mar;108(2):191-201.
  18297396
• Baird PN, Robman LD, Richardson AJ, Dimitrov PN, Tikellis G, McCarty CA, Guymer RH.Gene-environment interaction in progression of AMD: the CFH gene, smoking and exposure to chronic infection.Hum Mol Genet 2008 May 1;17(9):1299-305.
  18203751
• Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA.Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.Nature 2007 Oct 4;449(7162):557-63.
  17914389
• Ince TA, Richardson AL, Bell GW, Saitoh M, Godar S, Karnoub AE, Iglehart JD, Weinberg RA.Transformation of different human breast epithelial cell types leads to distinct tumor phenotypes.Cancer Cell 2007 Aug;12(2):160-70.
  17692807
• Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Rober
  17574021
• Mani SA, Yang J, Brooks M, Schwaninger G, Zhou A, Miura N, Kutok JL, Hartwell K, Richardson AL, Weinberg RA.Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers.Proc Natl Acad Sci U S A 2
  17537911
• Ganesan S, Richardson AL, Wang ZC, Iglehart JD, Miron A, Feunteun J, Silver D, Livingston DM.Abnormalities of the Inactive X Chromosome Are a Common Feature of BRCA1 Mutant and Sporadic Basal-like Breast Cancer.Cold Spring Harb Symp Quant Biol 2005;70:93-
  16869742
• Wang ZC, Buraimoh A, Iglehart JD, Richardson AL.Genome-Wide Analysis for Loss of Heterozygosity in Primary and Recurrent Phyllodes Tumor and Fibroadenoma of Breast using Single Nucleotide Polymorphism Arrays.Breast Cancer Res Treat 2006 Jun;97(3):301-9.
  16791486
• Bentires-Alj M, Gil SG, Chan R, Wang ZC, Wang Y, Imanaka N, Harris LN, Richardson A, Neel BG, Gu H.A role for the scaffolding adapter GAB2 in breast cancer.Nat Med 2006 Jan;12(1):114-21.
  16369543
• Alvarez JV, Febbo PG, Ramaswamy S, Loda M, Richardson A, Frank DA.Identification of a genetic signature of activated signal transducer and activator of transcription 3 in human tumors.Cancer Res 2005 Jun 15;65(12):5054-62.
  15958548
• Allinen M, Beroukhim R, Cai L, Brennan C, Lahti-Domenici J, Huang H, Porter D, Hu M, Chin L, Richardson A, Schnitt S, Sellers WR, Polyak K.Molecular characterization of the tumor microenvironment in breast cancer.Cancer Cell 2004 Jul;6(1):17-32.
  15261139
• Yang J, Mani SA, Donaher JL, Ramaswamy S, Itzykson RA, Come C, Savagner P, Gitelman I, Richardson A, Weinberg RA.Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis.Cell 2004 Jun 25;117(7):927-39.
  15210113
• Porter D, Weremowicz S, Chin K, Seth P, Keshaviah A, Lahti-Domenici J, Bae YK, Monitto CL, Merlos-Suarez A, Chan J, Hulette CM, Richardson A, Morton CC, Marks J, Duyao M, Hruban R, Gabrielson E, Gelman R, Polyak K.A neural survival factor is a candidate o
  12953101
• Hu Y, Hines LM, Weng H, Zuo D, Rivera M, Richardson A, LaBaer J.Analysis of genomic and proteomic data using advanced literature mining.J Proteome Res 2003 Jul-Aug;2(4):405-12.
  12938930
• Porter D, Lahti-Domenici J, Keshaviah A, Bae YK, Argani P, Marks J, Richardson A, Cooper A, Strausberg R, Riggins GJ, Schnitt S, Gabrielson E, Gelman R, Polyak K.Molecular markers in ductal carcinoma in situ of the breast.Mol Cancer Res 2003 Mar;1(5):362-
  12651909
• Seth P, Porter D, Lahti-Domenici J, Geng Y, Richardson A, Polyak K.Cellular and molecular targets of estrogen in normal human breast tissue.Cancer Res 2002 Aug 15;62(16):4540-4.
  12183401
• Geng Y, Yu Q, Whoriskey W, Dick F, Tsai KY, Ford HL, Biswas DK, Pardee AB, Amati B, Jacks T, Richardson A, Dyson N, Sicinski P.Expression of cyclins E1 and E2 during mouse development and in neoplasia.Proc Natl Acad Sci U S A 2001 Nov 6;98(23):13138-43.
  11687642