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G. Mike Makrigiorgos, PhD


  • Murphy DM, Rizaldos EC, Makrigiorgos GM.Enriching Mutant Sequences by Modulating the Denaturation Time during PCR.Clin Chem. 2014 May 5.
  • Castellanos-Rizaldos E, Milbury CA, Karatza E, Chen CC, Makrigiorgos GM, Merewood A.COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions.PLoS ONE. 2014;9(4):e94103.
  • Kumar R, Korideck H, Ngwa W, Berbeco RI, Makrigiorgos GM, Sridhar S.Third generation gold nanoplatform optimized for radiation therapy.Transl Cancer Res. 2013 Aug;2(4).
  • Castellanos-Rizaldos E, Milbury CA, Guha M, Makrigiorgos GM.COLD-PCR enriches low-level variant DNA sequences and increases the sensitivity of genetic testing.Methods Mol Biol. 2014;1102:623-39.
  • Kumar R, Kulkarni A, Nabulsi J, Nagesha DK, Cormack R, Makrigiorgos MG, Sridhar S.Facile Synthesis of PEGylated PLGA Nanoparticles Encapsulating Doxorubicin and its In Vitro Evaluation as Potent Drug Delivery Vehicle.Drug Deliv Transl Res. 2013 Aug 1;3(4):299-308.
  • Guha M, Castellanos-Rizaldos E, Makrigiorgos GM.DISSECT Method Using PNA-LNA Clamp Improves Detection of EGFR T790m Mutation.PLoS ONE. 2013;8(6):e67782.
  • Detappe A, Tsiamas P, Ngwa W, Zygmanski P, Makrigiorgos M, Berbeco R.The effect of flattening filter free delivery on endothelial dose enhancement with gold nanoparticles.Med Phys. 2013 Mar;40(3):031706.
  • Zygmanski P, Hoegele W, Tsiamas P, Cifter F, Ngwa W, Berbeco R, Makrigiorgos M, Sajo E.A stochastic model of cell survival for high-Z nanoparticle radiotherapy.Med Phys. 2013 Feb;40(2):024102.
  • Guha M, Castellanos-Rizaldos E, Liu P, Mamon H, Makrigiorgos GM.Differential strand separation at critical temperature: a minimally disruptive enrichment method for low-abundance unknown DNA mutations.Nucleic Acids Res. 2013 Feb 1;41(3):e50.
  • Tsiamas P, Liu B, Cifter F, Ngwa WF, Berbeco RI, Kappas C, Theodorou K, Marcus K, Makrigiorgos MG, Sajo E, Zygmanski P.Impact of beam quality on megavoltage radiotherapy treatment techniques utilizing gold nanoparticles for dose enhancement.Phys Med Biol. 2013 Feb 7;58(3):451-64.
  • Castellanos-Rizaldos E, Milbury CA, Makrigiorgos GM.Enrichment of mutations in multiple DNA sequences using COLD-PCR in emulsion.PLoS ONE. 2012;7(12):e51362.
  • Berbeco RI, Korideck H, Ngwa W, Kumar R, Patel J, Sridhar S, Johnson S, Price BD, Kimmelman A, Makrigiorgos GM.DNA Damage Enhancement from Gold Nanoparticles for Clinical MV Photon Beams.Radiat Res. 2012 Nov 13.
  • Ngwa W, Korideck H, Kassis AI, Kumar R, Sridhar S, Makrigiorgos GM, Cormack RA.In vitro radiosensitization by gold nanoparticles during continuous low-dose-rate gamma irradiation with I-125 brachytherapy seeds.Nanomedicine : Nanotechnology, Biology, And Medicine. 2012 Oct 2.
  • Castellanos-Rizaldos E, Liu P, Milbury CA, Guha M, Brisci A, Cremonesi L, Ferrari M, Mamon H, Makrigiorgos GM.Temperature-tolerant COLD-PCR reduces temperature stringency and enables robust mutation enrichment.Clin Chem. 2012 Jul;58(7):1130-8.
  • Milbury CA, Li J, Liu P, Makrigiorgos GM.COLD-PCR: improving the sensitivity of molecular diagnostics assays.Expert Rev Mol Diagn. 2011 Mar;11(2):159-69.
  • Milbury CA, Chen CC, Mamon H, Liu P, Santagata S, Makrigiorgos GM.Multiplex amplification coupled with COLD-PCR and high resolution melting enables identification of low-abundance mutations in cancer samples with low DNA content.J Mol Diagn. 2011 Mar;13(2):220-32.
  • Li J, J√§nne PA, Makrigiorgos GM.Biotinylated Probe Isolation of Targeted Gene Region Improves Detection of T790M Epidermal Growth Factor Receptor Mutation via Peptide Nucleic Acid-Enriched Real-Time PCR.Clin Chem. 2011 Feb 16.
  • Berbeco RI, Ngwa W, Makrigiorgos GM.Localized Dose Enhancement to Tumor Blood Vessel Endothelial Cells via Megavoltage X-rays and Targeted Gold Nanoparticles: New Potential for External Beam Radiotherapy.Int J Radiat Oncol Biol Phys. 2010 Dec 14.
  • Ngwa W, Makrigiorgos GM, Berbeco RI.Applying gold nanoparticles as tumor-vascular disrupting agents during brachytherapy: estimation of endothelial dose enhancement.Phys Med Biol. 2010 Nov 7;55(21):6533-48.
  • Milbury CA, Li J, Makrigiorgos GM.Ice-COLD-PCR enables rapid amplification and robust enrichment for low-abundance unknown DNA mutations.Nucleic Acids Res. 2010 Oct 11.
  • Nagesha DK, Tada DB, Stambaugh CK, Gultepe E, Jost E, Levy CO, Cormack R, Makrigiorgos GM, Sridhar S.Radiosensitizer-eluting nanocoatings on gold fiducials for biological in-situ image-guided radio therapy (BIS-IGRT).Phys Med Biol. 2010 Oct 21;55(20):6039-52.
  • Li J, Makrigiorgos GM.s-RT-MELT: a novel technology for mutation screening.Methods Mol Biol. 2010;653:207-19.
  • Zerilli F, Bonanno C, Shehi E, Amicarelli G, Adlerstein D, Makrigiorgos GM.Methylation-specific loop-mediated isothermal amplification for detecting hypermethylated DNA in simplex and multiplex formats.Clin Chem. 2010 Aug;56(8):1287-96.
  • Zhu X, Palmer MR, Makrigiorgos GM, Kassis AI.Solid-tumor radionuclide therapy dosimetry: new paradigms in view of tumor microenvironment and angiogenesis.Med Phys. 2010 Jun;37(6):2974-84.
  • Tada DB, Singh S, Nagesha D, Jost E, Levy CO, Gultepe E, Cormack R, Makrigiorgos GM, Sridhar S.Chitosan film containing poly(D,L-lactic-co-glycolic acid) nanoparticles: a platform for localized dual-drug release.Pharmaceutical Research. 2010 Aug;27(8):1738-45.
  • Cormack RA, Sridhar S, Suh WW, D'Amico AV, Makrigiorgos GM.Biological in situ dose painting for image-guided radiation therapy using drug-loaded implantable devices.Int J Radiat Oncol Biol Phys. 2010 Feb 1;76(2):615-23.
  • Milbury CA, Li J, Makrigiorgos GM.COLD-PCR-enhanced high-resolution melting enables rapid and selective identification of low-level unknown mutations.Clin Chem. 2009 Dec;55(12):2130-43.
  • Li J, Milbury CA, Li C, Makrigiorgos GM.Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma.Hum Mutat. 2009 Nov;30(11):1583-90.
  • Lang JE, Magbanua MJ, Scott JH, Makrigiorgos GM, Wang G, Federman S, Esserman LJ, Park JW, Haqq CM.A comparison of RNA amplification techniques at sub-nanogram input concentration.BMC Genomics. 2009;10:326.
  • Milbury CA, Li J, Makrigiorgos GM.PCR-based methods for the enrichment of minority alleles and mutations.Clin Chem. 2009 Apr;55(4):632-40. Review.
  • Li J, Makrigiorgos GM.COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.Biochem Soc Trans. 2009 Apr;37(Pt 2):427-32. Review.
  • Li J,Wang L,Janne PA,Makrigiorgos GM.Coamplification at lower denaturation temperature-PCR increases mutation-detection selectivity of TaqMan-based real-time PCR.Clin Chem. 2009 Apr;55(4):748-56.
  • Chin DW,Treister N,Friedland B,Cormack RA,Tishler RB,Makrigiorgos GM,Court LE.Effect of dental restorations and prostheses on radiotherapy dose distribution: a Monte Carlo study.J Appl Clin Med Phys. 2009;10(1):2853.
  • Mamon H,Hader C,Li J,Wang L,Kulke M,Amicarelli G,Shehi E,Adlerstein D,Roper K,Killion L,Hooshmand S,Makrigiorgos GM.Preferential amplification of apoptotic DNA from plasma: potential for enhancing detection of minor DNA alterations in circulating DNA.Clin Chem. 2008 Sep;54(9):1582-4.
  • Li J, Wang L, Mamon H, Kulke MH, Berbeco R, Makrigiorgos GM.Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.Nat Med. 2008 May;14(5):579-84.
  • Bonanno C, Shehi E, Adlerstein D, Makrigiorgos GM.MS-FLAG, a novel real-time signal generation method for methylation-specific PCR.Clin Chem. 2007 Dec;53(12):2119-27.
  • Amicarelli G, Shehi E, Makrigiorgos GM, Adlerstein D.FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations.Nucleic Acids Res. 2007;35(19):e131.
  • Wang F, Wang L, Briggs C, Sicinska E, Gaston SM, Mamon H, Kulke MH, Zamponi R, Loda M, Maher E, Ogino S, Fuchs CS, Li J, Hader C, Makrigiorgos GM.DNA degradation test predicts success in whole-genome amplification from diverse clinical samples.J Mol Diagn. 2007 Sep;9(4):441-51.
  • Li J, Berbeco R, Distel RJ, Jšnne PA, Wang L, Makrigiorgos GM.s-RT-MELT for rapid mutation scanning using enzymatic selection and real time DNA-melting: new potential for multiplex genetic analysis.Nucleic Acids Res. 2007;35(12):e84.
  • Xiang HF, Song JS, Chin DW, Cormack RA, Tishler RB, Makrigiorgos GM, Court LE, Chin LM.Build-up and surface dose measurements on phantoms using micro-MOSFET in 6 and 10 MV x-ray beams and comparisons with Monte Carlo calculations.Med Phys. 2007 Apr;34(4):1266-73.
  • Li J, Makrigiorgos GM.Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping.Nat Protoc. 2007;2(1):50-8.
  • Amicarelli G, Adlerstein D, Shehi E, Wang F, Makrigiorgos GM.Genotype-specific signal generation based on digestion of 3-way DNA junctions: application to KRAS variation detection.Clin Chem. 2006 Oct;52(10):1855-63.
  • Ogino S, Kawasaki T, Brahmandam M, Cantor M, Kirkner GJ, Spiegelman D, Makrigiorgos GM, Weisenberger DJ, Laird PW, Loda M, Fuchs CS.Precision and Performance Characteristics of Bisulfite Conversion and Real-Time PCR (MethyLight) for Quantitative DNA Methylation Analysis.J Mol Diagn. 2006 May;8(2):209-217.
  • Roy K, Wang L, Makrigiorgos GM, Price BD.Methylation of the ATM promoter in glioma cells alters ionizing radiation sensitivity.Biochem Biophys Res Commun. 2006 Jun 9;344(3):821-6.
  • Li J, Wang F, Mamon H, Kulke MH, Harris L, Maher E, Wang L, Makrigiorgos GM.Antiprimer quenching-based real-time PCR and its application to the analysis of clinical cancer samples.Clin Chem. 2006 Apr;52(4):624-33.
  • Li J, Harris L, Mamon H, Kulke MH, Liu WH, Zhu P, Mike Makrigiorgos G.Whole genome amplification of plasma-circulating DNA enables expanded screening for allelic imbalance in plasma.J Mol Diagn. 2006 Feb;8(1):22-30.
  • Wang F, Kaur M, Liu WH, Zhu P, Li J, Wang L, Makrigiorgos GM.High-throughput scanning of breast tumor surgical specimens for low-level mutations.Clin Chem Lab Med. 2005;43(8):810-6.
  • Wang G, Maher E, Brennan C, Chin L, Leo C, Kaur M, Zhu P, Rook M, Wolfe JL, Makrigiorgos GM.DNA amplification method tolerant to sample degradation.Genome Res. 2004 Nov;14(11):2357-66.
  • Wang G, Brennan C, Rook M, Wolfe JL, Leo C, Chin L, Pan H, Liu WH, Price B, Makrigiorgos GM.Balanced-PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples.Nucleic Acids Res. 2004 May;32(9):e76.
  • Chen S, Wang G, Makrigiorgos GM, Price BD.Stable siRNA-mediated silencing of ATM alters the transcriptional profile of HeLa cells.Biochem Biophys Res Commun. 2004 May 14;317(4):1037-44.
  • Liu WH, Kaur M, Wang G, Zhu P, Zhang Y, Makrigiorgos GM.Inverse PCR-based RFLP scanning identifies low-level mutation signatures in colon cells and tumors.Cancer Res. 2004 Apr 1;64(7):2544-51.
  • Liu WH, Makrigiorgos GM.Sensitive and quantitative detection of mutations associated with clinical resistance to STI-571.Leuk Res. 2003 Nov;27(11):979-82.
  • Liu WH, Kaur M, Makrigiorgos GM.Detection of hotspot mutations and polymorphisms using an enhanced PCR-RFLP approach.Hum Mutat. 2003 May;21(5):535-41.
  • Kaur M, Makrigiorgos GM.Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA.Nucleic Acids Res. 2003 Mar 15;31(6):e26.
  • Xue LY, Butler NJ, Makrigiorgos GM, Adelstein SJ, Kassis AI.Bystander effect produced by radiolabeled tumor cells in vivo.Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13765-70.
  • Kaur M, Zhang Y, Liu WH, Tetradis S, Price BD, Makrigiorgos GM.Ligation of a primer at a mutation: a method to detect low level mutations in DNA.Mutagenesis. 2002 Sep;17(5):365-74.
  • Makrigiorgos GM, Chakrabarti S, Zhang Y, Kaur M, Price BD.A PCR-based amplification method retaining the quantitative difference between two complex genomes.Nat Biotechnol. 2002 Sep;20(9):936-9.
  • Maulik G, Salgia R, Makrigiorgos GM.Flow cytometric determination of lipid peroxidation using fluoresceinated phosphoethanolamine.Methods Enzymol. 2002;352:80-91.
  • Zhang Y, Kaur M, Price BD, Tetradis S, Makrigiorgos GM.An amplification and ligation-based method to scan for unknown mutations in DNA.Hum Mutat. 2002 Aug;20(2):139-47.
  • Zhang Y, Price BD, Tetradis S, Chakrabarti S, Maulik G, Makrigiorgos GM.Reproducible and inexpensive probe preparation for oligonucleotide arrays.Nucleic Acids Res. 2001 Jul 1;29(13):E66-6.
  • Chakrabarti S, Mahmood A, Makrigiorgos GM.Fluorescent labelling of closely-spaced aldehydes induced in DNA by bleomycin-Fe(III).Int J Radiat Biol. 1999 Aug;75(8):1055-65.
  • Chakrabarti S, Price BD, Tetradis S, Fox EA, Zhang Y, Maulik G, Makrigiorgos GM.Highly selective isolation of unknown mutations in diverse DNA fragments: toward new multiplex screening in cancer.Cancer Res. 2000 Jul 15;60(14):3732-7.
  • Makrigiorgos GM.Detection of chromatin-associated hydroxyl radicals generated by DNA-bound metal compounds and antitumor antibiotics.Met Ions Biol Syst. 1999;36:521-45.
  • Maulik G, Botchway S, Chakrabarti S, Tetradis S, Price B, Makrigiorgos GM.Novel non-isotopic detection of MutY enzyme-recognized mismatches in DNA via ultrasensitive detection of aldehydes.Nucleic Acids Res. 1999 Mar 1;27(5):1316-22.
  • Maulik G, Kassis AI, Savvides P, Makrigiorgos GM.Fluoresceinated phosphoethanolamine for flow-cytometric measurement of lipid peroxidation.Free Radic Biol Med. 1998 Oct;25(6):645-53.
  • Makrigiorgos GM, Chakrabarti S, Mahmood A.Fluorescent labelling of abasic sites: a novel methodology to detect closely-spaced damage sites in DNA.Int J Radiat Biol. 1998 Jul;74(1):99-109.
  • Kassis AI, Wen PY, Van den Abbeele AD, Baranowska-Kortylewicz J, Makrigiorgos GM, Metz KR, Matalka KZ, Cook CU, Sahu SK, Black PM, Adelstein SJ.5-[125I]iodo-2'-deoxyuridine in the radiotherapy of brain tumors in rats.J Nucl Med. 1998 Jul;39(7):1148-54.
  • Botchway SW, Chakrabarti S, Makrigiorgos GM.Novel visible and ultraviolet light photogeneration of hydroxyl radicals by 2-methyl-4-nitro-quinoline-N-oxide (MNO) and 4,4'-dinitro-(2,2')bipyridinyl-N,N'-dioxide (DBD).Photochem Photobiol. 1998 Jun;67(6):635-40.
  • Chakrabarti S, Kassis AI, Slayter HS, Bump EA, Sahu SK, Makrigiorgos GM.Continuous detection of radiation or metal generated hydroxyl radicals within core chromatin particles.Int J Radiat Biol. 1998 Jan;73(1):53-63.
  • Makrigiorgos GM.Detection of lipid peroxidation on erythrocytes using the excimer-forming property of a lipophilic BODIPY fluorescent dye.J Biochem Biophys Methods. 1997 Aug 1;35(1):23-35.
  • Makrigiorgos GM, Kassis AI, Mahmood A, Bump EA, Savvides P.Novel fluorescein-based flow-cytometric method for detection of lipid peroxidation.Free Radic Biol Med. 1997;22(1-2):93-100.
  • Chakrabarti S, Mahmood A, Kassis AI, Bump EA, Jones AG, Makrigiorgos GM.Generation of hydroxyl radicals by nucleohistone-bound metal-adriamycin complexes.Free Radic Res. 1996 Sep;25(3):207-20.
  • Chakrabarti S, Makrigiorgos GM, O'Brien K, Bump E, Kassis AI.Measurement of hydroxyl radicals catalyzed in the immediate vicinity of DNA by metal-bleomycin complexes.Free Radic Biol Med. 1996;20(6):777-83.
  • Makrigiorgos GM, Bump E, Huang C, Baranowska-Kortylewicz J, Kassis AI.A fluorimetric method for the detection of copper-mediated hydroxyl free radicals in the immediate proximity of DNA.Free Radic Biol Med. 1995 Apr;18(4):669-78.
  • Sahu SK, Kassis AI, Makrigiorgos GM, Baranowska-Kortylewicz J, Adelstein SJ.The effects of indium-111 decay on pBR322 DNA.Radiat Res. 1995 Feb;141(2):193-8.
  • Collins AK, Makrigiorgos GM, Svensson GK.Coumarin chemical dosimeter for radiation therapy.Med Phys. 1994 Nov;21(11):1741-7.
  • Makrigiorgos GM, Bump E, Huang C, Baranowska-Kortylewicz J, Kassis AI.Accessibility of nucleic acid-complexed biomolecules to hydroxyl radicals correlates with their conformation: a fluorescence polarization spectroscopy study.Int J Radiat Biol. 1994 Sep;66(3):247-57.
  • Makrigiorgos GM, Folkard M, Huang C, Bump E, Baranowska-Kortylewicz J, Sahu SK, Michael BD, Kassis AI.Quantification of radiation-induced hydroxyl radicals within nucleohistones using a molecular fluorescent probe.Radiat Res. 1994 May;138(2):177-85.
  • Makrigiorgos GM, Baranowska-Kortylewicz J, Bump E, Sahu SK, Berman RM, Kassis AI.A method for detection of hydroxyl radicals in the vicinity of biomolecules using radiation-induced fluorescence of coumarin.Int J Radiat Biol. 1993 Apr;63(4):445-58.
  • Makrigiorgos GM, Berman RM, Baranowska-Kortylewicz J, Bump E, Humm JL, Adelstein SJ, Kassis AI.DNA damage produced in V79 cells by DNA-incorporated iodine-123: a comparison with iodine-125.Radiat Res. 1992 Mar;129(3):309-14.
  • Baranowska-Kortylewicz J, Makrigiorgos GM, Van den Abbeele AD, Berman RM, Adelstein SJ, Kassis AI.5-[123I]iodo-2'-deoxyuridine in the radiotherapy of an early ascites tumor model.Int J Radiat Oncol Biol Phys. 1991 Nov;21(6):1541-51.
  • Makrigiorgos GM, Ito S, Baranowska-Kortylewicz J, Vinter DW, Iqbal A, Van den Abbeele AD, Adelstein SJ, Kassis AI.Inhomogeneous deposition of radiopharmaceuticals at the cellular level: experimental evidence and dosimetric implications.J Nucl Med. 1990 Aug;31(8):1358-63.
  • Makrigiorgos GM, Adelstein SJ, Kassis AI.Cellular radiation dosimetry and its implications for estimation of radiation risks. Illustrative results with technetium 99m-labeled microspheres and macroaggregates.JAMA. 1990 Aug 1;264(5):592-5.
  • Makrigiorgos G, Adelstein SJ, Kassis AI.Auger electron emitters: insights gained from in vitro experiments.Radiat Environ Biophys. 1990;29(2):75-91.
  • Makrigiorgos GM, Smathers JB, Myers LT, Benedict SH.Radiation quality investigations around a neutron therapy beam, using a high-pressure ionization chamber.Med Phys. 1990 Jan-Feb;17(1):84-6.
  • Makrigiorgos GM, Adelstein SJ, Kassis AI.Limitations of conventional internal dosimetry at the cellular level.J Nucl Med. 1989 Nov;30(11):1856-64.
  • Makrigiorgos GM.Derivation of radiation quality average parameters in neutron-gamma radiation fields with the high-pressure ionization chamber: theory and practice.Radiat Res. 1989 Jun;118(3):387-400.
  • Makrigiorgos GM, Kassis AI, Baranowska-Kortylewicz J, McElvany KD, Welch MJ, Sastry KS, Adelstein SJ.Radiotoxicity of 5-[123I]iodo-2'-deoxyuridine in V79 cells: a comparison with 5-[125I]iodo-2'-deoxyuridine.Radiat Res. 1989 Jun;118(3):532-44.
  • Folkard M, Makrigiorgos G, Roper MJ, Waker AJ, Michael BD.Measurements of neutron energy using a recoil-proton telescope and a high-pressure ionization chamber.Radiat Res. 1989 Apr;118(1):37-45.
  • Makrigiorgos G, Antonadou D, Proukakis C, Throuvalas N.Measurement of the restricted linear energy transfer of stray radiation close to the treatment volume of 12 and 18 MeV clinical photon beams.Med Phys. 1989 Mar-Apr;16(2):302-4.
  • Makrigiorgos G.Characteristics of an ethylene-polyethylene high-pressure ionization chamber and its potential for deriving radiation dose and quality information in neutron-gamma radiation fields.Med Phys. 1988 Jan-Feb;15(1):36-9.
  • Lymberis C, Makrigiorgos G, Sbonias E, Polizois E, Mortzos G, Bacas A, Fountos A.Radiocesium levels in human muscle samples in Greece one year after the Chernobyl accident.Int J Rad Appl Instrum [A]. 1988;39(2):175-6.
  • Makrigiorgos G, Antonadou D.Measurement of the restricted dose mean LET outside the primary beam of a 60Co radiotherapy unit.Radiat Res. 1987 Apr;110(1):142-8.
  • Makrigiorgos G, Waker AJ.The measurement of absorbed dose and dose-equivalent levels for in vivo neutron activation analysis.Phys Med Biol. 1985 Sep;30(9):909-19.