Collaborative Functional Genomics Core

Notable References

A large number of RNAi screens conducted with the participating groups have resulted in data included in publications in high-profile journals. In addition, the core's scientific leaders and center staff members have contributed numerous review articles, chapters and methods papers. It is not practical to list all of these here but we will try to help you get started.

Selected Reviews and Methods Papers from the Core Leadership

Hartenian E, Doench JG. (2015) Genetic screens and functional genomics using CRISPR/Cas9 technology. FEBS J. 282(8):1383-93. Review.PubMed

Vinayagam et al. (2013) Protein complex-based analysis framework for high-throughput data sets. Sci Signal. 6(264):rs5. PubMed

Mohr, Smith, Shamu, Neumuller and Perrimon (2014) RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol. 15(9):591-600. Pubmed

Yilmazel et al. (2014) Online GESS: Prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis. BMC Bioinformatics. 15:192. Pubmed

Hu et al. (2013) UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents. Genetics. 195(1):37-45. Pubmed

Mohr et al. (2010) Genomic Screening with RNAi: Results and Challenges. Ann. Rev. Biochem. Vol. 79. Pubmed

Birmingham et al. (2009) Statistical methods for analysis of high throughput RNA interference screens. Nat Methods. 6(8):569-75. Pubmed

Hahn et al. (2009) Integrative genomic approaches to understanding cancer. Biochim Biophys Acta. 1790(6):478-84. Article

Root et al. (2006) Genome-scale loss-of-function screening with a lentiviral RNAi library. Nat Methods. 3(9):715-9. Pubmed

 

Publications Relevant to Specific Participating Centers

Also Relevant

Detailed information about protocols, vectors, etc. are available on-line at the Broad Genome Perturbation Platform web site.

Detailed information about screen assay development, screening and data analysis are available on-line at the HMS ICCB Longwood Screening Facility web site (see screening guidelines, helpful publications & links, and other pages).

For the latest in fly RNAi screening and related topics, check out the DRSC Director’s Blog.