Collaborative Functional Genomics Core
A large number of RNAi screens conducted with the participating groups have resulted in data included in publications in high-profile journals. In addition, the core's scientific leaders and center staff members have contributed numerous review articles, chapters and methods papers. It is not practical to list all of these here but we will try to help you get started.
Selected Reviews and Methods Papers from the Core Leadership
Hartenian E, Doench JG. (2015) Genetic screens and functional genomics using CRISPR/Cas9 technology. FEBS J. 282(8):1383-93. Review.PubMed
Vinayagam et al. (2013) Protein complex-based analysis framework for high-throughput data sets. Sci Signal. 6(264):rs5. PubMed
Mohr, Smith, Shamu, Neumuller and Perrimon (2014) RNAi screening comes of age: improved techniques and complementary approaches. Nat Rev Mol Cell Biol. 15(9):591-600. Pubmed
Yilmazel et al. (2014) Online GESS: Prediction of miRNA-like off-target effects in large-scale RNAi screen data by seed region analysis. BMC Bioinformatics. 15:192. Pubmed
Hu et al. (2013) UP-TORR: online tool for accurate and Up-to-Date annotation of RNAi Reagents. Genetics. 195(1):37-45. Pubmed
Mohr et al. (2010) Genomic Screening with RNAi: Results and Challenges. Ann. Rev. Biochem. Vol. 79. Pubmed
Birmingham et al. (2009) Statistical methods for analysis of high throughput RNA interference screens. Nat Methods. 6(8):569-75. Pubmed
Hahn et al. (2009) Integrative genomic approaches to understanding cancer. Biochim Biophys Acta. 1790(6):478-84. Article
Root et al. (2006) Genome-scale loss-of-function screening with a lentiviral RNAi library. Nat Methods. 3(9):715-9. Pubmed
Publications Relevant to Specific Participating Centers
- Publications with data obtained at the DRSC, listed by year or by topic.
- Publications from the Elledge Lab
- Publications with data obtained at the ICCB Longwood Screening Facility
Detailed information about protocols, vectors, etc. are available on-line at the Broad Genome Perturbation Platform web site.
Detailed information about screen assay development, screening and data analysis are available on-line at the HMS ICCB Longwood Screening Facility web site (see screening guidelines, helpful publications & links, and other pages).
For the latest in fly RNAi screening and related topics, check out the DRSC Director’s Blog.