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Core A: Tissue and Pathology

The availability of appropriately prepared, preserved and clinically-annotated specimens from patients with lung cancer is critical to the work proposed in this SPORE. Two large banks of archival material exist within the participating institutions. However, these have been collected for different purposes using different protocols. Many of them are anonymous specimens collected as discarded tissue, and lack the ability to link information pertinent to their analysis. Therefore, new protocols have been generated to address these issues. Informed consent is obtained for specimen use and linkage of relevant clinical information, including outcome. Specimens will be prospectively collected for the SPORE using these protocols by the banks at Brigham and Women's Hospital (BWH) and the Massachusetts General Hospital (MGH). The specimens and information will be catalogued and linked using a secure database system developed at the Dana Farber Cancer Institute (DFCI). Frozen and paraffin histology will be provided both to characterize banked human tumor specimens and to evaluate rodent tumors. Fine needle aspirates of tumors will be obtained and evaluated cytologically. Immunoperoxidase staining and scoring will be performed for both rodent and human specimens.

The Tissue and Pathology Core has two specific aims: 

  • The first aim is to provide a repository of tissue and blood specimens for use by SPORE investigators.  Blood specimens will be collected from patients enrolled on clinical trials associated with projects.  Archival specimens from BWH and MGH banks not linked to clinical data will be provided anonymously to projects for pilot work.  A Specimen Resource Utilization Committee will facilitate the processing and distribution of tissues from the SPORE Specimen Bank. 
  • The second aim is to provide pathology services to SPORE projects.  These will include routine histology, cytology, immunohistochemistry, and in situ hybridization, as well as intrepretation, diagnosis, description and scoring of pathologic materials.  Rodent pathology services will be provided to projects utilizing mouse models.