SPOREs
Ovarian
Projects
Project 2: Genetic changes in early stage ovarian cancer
Specific Aims
- Identify DNA copy number abnormalities (CNA) and differential gene expression in ovarian cancer by cDNA array
- Perform allelotyping on a series of early stage ovarian cancer samples using the single nucleotide polymorphism (SNP)-array-based LOH analysis
- Select and validate candidate genes, which may be used as diagnostic markers for early detection of ovarian cancer
- Delineate the pathogenetic pathways and identify prognostic markers for ovarian cancer
Publications
Bonome T, Lee JY, Park DC, Radonovich M, Pise-Masison C, Brady J, Gardner GJ, Hao K, Wong WH, Barrett JC, et al. "Expression profiling of serous low malignant potential, low-grade, and high-grade tumors of the ovary." Cancer Res 2005; 65(22):pg:10602-10612. PMCID: 16288054*
Birrer MJ, Johnson ME, Hao K, Wong KK, Park DC, Bell A, Welch WR, Berkowitz RS, and Mok SC. "Whole genome oligonucleotide-based array comparative genomic hybridization analysis identified fibroblast growth factor 1 as a prognostic marker for advanced-stage serous ovarian adenocarcinomas." J Clin Oncol 2007; 25(16):pg:2281-2287. PMCID: 17538174
Gagnon A, Kim JH, Schorge JO, Ye B, Liu B, Hasselblatt K, Welch WR, Bandera CA, and Mok SC. "Use of a combination of approaches to identify and validate relevant tumor-associated antigens and their corresponding autoantibodies in ovarian cancer patients." Clin Cancer Res 2008; 14(3):pg:764-771. PMCID: 18245537
Huddleston HG, Wong KK, Welch WR, Berkowitz RS, and Mok SC. "Clinical applications of microarray technology: creatine kinase B is an up-regulated gene in epithelial ovarian cancer and shows promise as a serum marker." Gynecol Oncol 2005; 96(1):pg:77-83. PMCID: 15589584*
Koon EC, Ma PC, Salgia R, Welch WR, Christensen JG, Berkowitz RS, and Mok SC. "Effect of a c-Met-specific, ATP-competitive small-molecule inhibitor SU11274 on human ovarian carcinoma cell growth, motility, and invasion." Int J Gynecol Cancer 2007. PMCID: 18021219
Kwong J, Lee JY, Wong KK, Zhou X, Wong DT, Lo KW, Welch WR, Berkowitz RS, and Mok SC. "Candidate tumor-suppressor gene DLEC1 is frequently downregulated by promoter hypermethylation and histone hypoacetylation in human epithelial ovarian cancer." Neoplasia 2006; 8(4):pg:268-278. PMCID: 16756719*
Lee S, Garner EI, Welch WR, Berkowitz RS, and Mok SC. "Over-expression of hypoxia-inducible factor 1 alpha in ovarian clear cell carcinoma." Gynecol Oncol 2007; 106(2):pg:311-317. PMCID: 17532031
Litkouhi B, Litkouhi B, Fleming E, Welch WR, Berkowitz RS, Birrer MJ, and Mok SC. "Overexpression of CEACAM6 in borderline and invasive mucinous ovarian neoplasms." Gynecol Oncol 2008; 109(2):pg:234-239. PMCID: 18331757
Litkouhi B, Kwong J, Lo CM, Smedley JG, 3rd, McClane BA, Aponte M, Gao Z, Sarno JL, Hinners J, Welch WR, et al. "Claudin-4 overexpression in epithelial ovarian cancer is associated with hypomethylation and is a potential target for modulation of tight junction barrier function using a C-terminal fragment of Clostridium perfringens enterotoxin." Neoplasia 2007; 9(4):pg:304-314. PMCID: 17460774
Mok SC, Elias KM, Wong KK, Ho K, Bonome T, and Birrer MJ. "Biomarker discovery in epithelial ovarian cancer by genomic approaches." Adv Cancer Res 2007; 96:pg:1-22. PMCID: 17161674+
Mok SC, Kwong J, Welch WR, Samimi G, Ozbun L, Bonome T, Birrer MJ, Berkowitz RS, and Wong KK. "Etiology and pathogenesis of epithelial ovarian cancer." Dis Markers 2007; 23(5-6):pg:367-376. PMCID: 18057520
Nishimura S, Tsuda H, Ito K, Jobo T, Yaegashi N, Inoue T, Sudo T, Berkowitz RS, and Mok SC. "Differential expression of ABCF2 protein among different histologic types of epithelial ovarian cancer and in clear cell adenocarcinomas of different organs." Hum Pathol 2007; 38(1):pg:134-139. PMCID: 16996567*
Samimi G, Ozbun L, Johnson M, Mok S, and Birrer MJ. "Biomarkers of mucinous tumors of the ovary." Disease Biomarker 2007; 23:pg:377-387. PMCID: 18057522
Schorge JO, Drake RD, Lee H, Skates SJ, Rajanbabu R, Miller DS, Kim JH, Cramer DW, Berkowitz RS, and Mok SC. "Osteopontin as an adjunct to CA125 in detecting recurrent ovarian cancer." Clin Cancer Res 2004; 10(10):pg:3474-3478. PMCID: 15161704*
Tsuda H, Birrer MJ, Ito YM, Ohashi Y, Lin M, Lee C, Wong WH, Rao PH, Lau CC, Berkowitz RS, et al. "Identification of DNA copy number changes in microdissected serous ovarian cancer tissue using a cDNA microarray platform." Cancer Genet Cytogenet 2004; 155(2):pg:97-107. PMCID: 15571795*
Tsuda H, Bandera CA, Birrer MJ, Hashiguchi Y, Berkowitz RS, and Mok SC. "Cyclin E amplification and overexpression in clear cell adenocarcinoma of the ovary." Oncology 2004; 67(3-4):pg:291-299. PMCID: 15557791*
Tsuda H, Ito YM, Ohashi Y, Wong KK, Hashiguchi Y, Welch WR, Berkowitz RS, Birrer MJ, and Mok SC. "Identification of overexpression and amplification of ABCF2 in clear cell ovarian adenocarcinomas by cDNA microarray analyses." Clin Cancer Res 2005; 11(19 Pt 1):pg:6880-6888. PMCID: 16203778*
Wang V, Li C, Lin M, Welch W, Bell D, Wong YF, Berkowitz R, Mok SC, and Bandera CA. "Ovarian cancer is a heterogeneous disease." Cancer Genet Cytogenet 2005; 161(2):pg:170-173. PMCID: 16102589*
Ye B, Gagnon A, and Mok SC. "Recent technical strategies to identify diagnostic biomarkers for ovarian cancer." Expert Rev Proteomics 2007; 4(1):pg:121-131. PMCID: 17288520
Ye B, Skates S, Mok SC, Horick NK, Rosenberg HF, Vitonis A, Edwards D, Sluss P, Han WK, Berkowitz RS, et al. "Proteomic-based discovery and characterization of glycosylated eosinophil-derived neurotoxin and COOH-terminal osteopontin fragments for ovarian cancer in urine." Clin Cancer Res 2006; 12(2):pg:432-441. PMCID: 16428483
Comments: Dr. Samuel Mok accepted a Professorship at MD Anderson as of June 2008. Drs. Sobel and Blessing have completed their work. For the new Project 2 in the continuation application, Dr. Ronny Drapkin of Dana-Farber Cancer Institute will describe the molecular changes that occur within the secretory cells of the Fallopian tubes that may lead to the occurrence of high grade serous cancers.
