Photo of Charles P. Lin, PhD

Charles P. Lin, PhD

Massachusetts General Hospital

Massachusetts General Hospital
Phone: (617) 724-3957


charles_lin@hms.harvard.edu

Photo of Charles P. Lin, PhD

Massachusetts General Hospital
Phone: (617) 724-3957


charles_lin@hms.harvard.edu

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Charles P. Lin, PhD

Massachusetts General Hospital

EDUCATIONAL TITLES

  • Associate Professor, Dermatology, Harvard Medical School
  • Associate Biophysicist, Dermatology, Massachusetts General Hospital

Research Abstract

Dr. Lin leads an Advanced Microscopy Group in the MGH Center for Systems Biology and Wellman Center for Photomedicine, where cutting-edge optical imaging techniques are being developed for in vivo cell tracking and molecular imaging studies. The research group is composed of a multidisciplinary team of optical engineers, physicists, biologist and chemist all working together to advance our understanding of the in vivo behavior of immune cells, cancer cells, and stem cells in disease models and in transplantation settings. There are several custom-built confocal and two photon hybrid microscopes in Dr. Lin's laboratory, each one tailored for a specific live animal imaging applications. A new system is currently being developed for image-guided laser microsurgery. Dr. Lin has also developed an in vivo flow cytometer for real-time detection and quantification of fluorescent cells in the circulation without the need to draw blood samples.

Publications from Harvard Catalyst Profiles

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  • Man CH, Mercier FE, Liu N, Dong W, Stephanopoulos G, Jiang L, Jung Y, Lin C, Leung AY, Scadden DT. Proton export alkalinizes intracellular pH and reprograms carbon metabolism to drive hematopoietic progenitor growth. Blood 2021. PubMed
  • Hou J, Lin CP, Intini G. Activation of creER recombinase in the mouse calvaria induces local recombination without effects on distant skeletal segments. Sci Rep 2021; 11:8214. PubMed
  • Oki T, Mercier F, Kato H, Jung Y, McDonald TO, Spencer JA, Mazzola MC, van Gastel N, Lin CP, Michor F, Kitamura T, Scadden DT. Imaging dynamic mTORC1 pathway activity in vivo reveals marked shifts that support time-specific inhibitor therapy in AML. Nat Commun 2021; 12:245. PubMed
  • Wu JW, Jung Y, Yeh SA, Seo Y, Runnels JM, Burns CS, Mizoguchi T, Ito K, Spencer JA, Lin CP. Intravital fluorescence microscopy with negative contrast. PLoS ONE 2021; 16:e0255204. PubMed
  • Christodoulou C, Spencer JA, Yeh SA, Turcotte R, Kokkaliaris KD, Panero R, Ramos A, Guo G, Seyedhassantehrani N, Esipova TV, Vinogradov SA, Rudzinskas S, Zhang Y, Perkins AS, Orkin SH, Calogero RA, Schroeder T, Lin CP, Camargo FD. Live-animal imaging of native haematopoietic stem and progenitor cells. Nature 2020; 578:278-283. PubMed
  • Scaramozza A, Park D, Kollu S, Beerman I, Sun X, Rossi DJ, Lin CP, Scadden DT, Crist C, Brack AS. Lineage Tracing Reveals a Subset of Reserve Muscle Stem Cells Capable of Clonal Expansion under Stress. Cell Stem Cell 2019; 24:944-957.e5. PubMed
  • Tan X, Patil R, Bartosik P, Runnels JM, Lin CP, Niedre M. In Vivo Flow Cytometry of Extremely Rare Circulating Cells. Sci Rep 2019; 9:3366. PubMed
  • Patil R, Tan X, Bartosik P, Detappe A, Runnels JM, Ghobrial I, Lin CP, Niedre M. Fluorescence monitoring of rare circulating tumor cell and cluster dissemination in a multiple myeloma xenograft model in vivo. J Biomed Opt 2019; 24:1-11. PubMed
  • Shao L, Chang J, Feng W, Wang X, Williamson EA, Li Y, Schajnovitz A, Scadden D, Mortensen LJ, Lin CP, Li L, Paulson A, Downing J, Zhou D, Hromas RA. The Wave2 scaffold Hem-1 is required for transition of fetal liver hematopoiesis to bone marrow. Nat Commun 2018; 9:2377. PubMed
  • Yeh SA, Wilk K, Lin CP, Intini G. In Vivo 3D Histomorphometry Quantifies Bone Apposition and Skeletal Progenitor Cell Differentiation. Sci Rep 2018; 8:5580. PubMed
  • Jung Y, Spencer JA, Raphael AP, Wu JW, Alt C, Runnels JR, Geiger B, Lin CP. Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability. Methods Mol Biol 2018; 1763:11-22. PubMed
  • Hartmann C, Patil R, Lin CP, Niedre M. Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects. Phys Med Biol 2017; 63:01TR01. PubMed
  • Hartmann C, Patil R, Lin CP, Niedre MJ. Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects. Phys Med Biol 2017. PubMed
  • Turcotte R, Alt C, Runnels JM, Ito K, Wu JW, Zaher W, Mortensen LJ, Silberstein L, Côté DC, Kung AL, Ito K, Lin CP. Image-guided transplantation of single cells in the bone marrow of live animals. Sci Rep 2017; 7:3875. PubMed
  • Lee J, Dykstra B, Spencer JA, Kenney LL, Greiner DL, Shultz LD, Brehm MA, Lin CP, Sackstein R, Rossi DJ. mRNA-mediated glycoengineering ameliorates deficient homing of human stem cell-derived hematopoietic progenitors. J Clin Invest 2017; 127:2433-2437. PubMed
  • Pera V, Tan X, Runnels J, Sardesai N, Lin CP, Niedre M. Diffuse fluorescence fiber probe for in vivo detection of circulating cells. J Biomed Opt 2017; 22:37004. PubMed
  • Ito K, Turcotte R, Cui J, Zimmerman SE, Pinho S, Mizoguchi T, Arai F, Runnels JM, Alt C, Teruya-Feldstein J, Mar JC, Singh R, Suda T, Lin CP, Frenette PS, Ito K. Self-renewal of a purified Tie2+ hematopoietic stem cell population relies on mitochondrial clearance. Science 2016; 354:1156-1160. PubMed
  • Yu VW, Yusuf RZ, Oki T, Wu J, Saez B, Wang X, Cook C, Baryawno N, Ziller MJ, Lee E, Gu H, Meissner A, Lin CP, Kharchenko PV, Scadden DT. Epigenetic Memory Underlies Cell-Autonomous Heterogeneous Behavior of Hematopoietic Stem Cells. Cell 2016; 167:1310-1322.e17. PubMed
  • Silberstein L, Goncalves KA, Kharchenko PV, Turcotte R, Kfoury Y, Mercier F, Baryawno N, Severe N, Bachand J, Spencer JA, Papazian A, Lee D, Chitteti BR, Srour EF, Hoggatt J, Tate T, Lo Celso C, Ono N, Nutt S, Heino J, Sipilä K, Shioda T, Osawa M, Lin CP, Hu GF, Scadden DT. Proximity-Based Differential Single-Cell Analysis of the Niche to Identify Stem/Progenitor Cell Regulators. Cell Stem Cell 2016; 19:530-543. PubMed
  • Turcotte R, Wu JW, Lin CP. Intravital multiphoton photoconversion with a cell membrane dye. J Biophotonics 2016. PubMed
  • Dykstra B, Lee J, Mortensen LJ, Yu H, Wu ZL, Lin CP, Rossi DJ, Sackstein R. Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells. Stem Cells 2016. PubMed
  • Itkin T, Gur-Cohen S, Spencer JA, Schajnovitz A, Ramasamy SK, Kusumbe AP, Ledergor G, Jung Y, Milo I, Poulos MG, Kalinkovich A, Ludin A, Kollet O, Shakhar G, Butler JM, Rafii S, Adams RH, Scadden DT, Lin CP, Lapidot T. Distinct bone marrow blood vessels differentially regulate haematopoiesis. Nature 2016; 532:323-8. PubMed
  • Yang Z, Concannon J, Ng KS, Seyb K, Mortensen LJ, Ranganath S, Gu F, Levy O, Tong Z, Martyn K, Zhao W, Lin CP, Glicksman MA, Karp JM. Tetrandrine identified in a small molecule screen to activate mesenchymal stem cells for enhanced immunomodulation. Sci Rep 2016; 6:30263. PubMed
  • Wu JW, Turcotte R, Alt C, Runnels JM, Tsao H, Lin CP. Defining Clonal Color in Fluorescent Multi-Clonal Tracking. Sci Rep 2016; 6:24303. PubMed
  • Li HW, Andreola G, Carlson AL, Shao S, Lin CP, Zhao G, Sykes M. Rapid Functional Decline of Activated and Memory Graft-versus-Host-Reactive T Cells Encountering Host Antigens in the Absence of Inflammation. J Immunol 2015; 195:1282-92. PubMed
  • Levy O, Mortensen LJ, Boquet G, Tong Z, Perrault C, Benhamou B, Zhang J, Stratton T, Han E, Safaee H, Musabeyezu J, Yang Z, Multon MC, Rothblatt J, Deleuze JF, Lin CP, Karp JM. A small-molecule screen for enhanced homing of systemically infused cells. Cell Rep 2015; 10:1261-8. PubMed
  • Teo GS, Yang Z, Carman CV, Karp JM, Lin CP. Intravital imaging of mesenchymal stem cell trafficking and association with platelets and neutrophils. Stem Cells 2015. PubMed
  • Saez B, Ferraro F, Yusuf RZ, Cook CM, Yu VW, Pardo-Saganta A, Sykes SM, Palchaudhuri R, Schajnovitz A, Lotinun S, Lymperi S, Mendez-Ferrer S, Toro RD, Day R, Vasic R, Acharya SS, Baron R, Lin CP, Yamaguchi Y, Wagers AJ, Scadden DT. Inhibiting stromal cell heparan sulfate synthesis improves stem cell mobilization and enables engraftment without cytotoxic conditioning. Blood 2014. PubMed
  • Turcotte R, Alt C, Mortensen LJ, Lin CP. Characterization of multiphoton microscopy in the bone marrow following intravital laser osteotomy. Biomed Opt Express 2014; 5:3578-88. PubMed
  • Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, Yu M, Pely A, Engstrom A, Zhu H, Brannigan BW, Kapur R, Stott SL, Shioda T, Ramaswamy S, Ting DT, Lin CP, Toner M, Haber DA, Maheswaran S. Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell 2014; 158:1110-22. PubMed
  • Alt C, Runnels JM, Mortensen LJ, Zaher W, Lin CP. In vivo imaging of microglia turnover in the mouse retina after ionizing radiation and dexamethasone treatment. Invest Ophthalmol Vis Sci 2014; 55:5314-9. PubMed
  • Ksander BR, Kolovou PE, Wilson BJ, Saab KR, Guo Q, Ma J, McGuire SP, Gregory MS, Vincent WJ, Perez VL, Cruz-Guilloty F, Kao WW, Call MK, Tucker BA, Zhan Q, Murphy GF, Lathrop KL, Alt C, Mortensen LJ, Lin CP, Zieske JD, Frank MH, Frank NY. ABCB5 is a limbal stem cell gene required for corneal development and repair. Nature 2014; 511:353-7. PubMed
  • Heidt T, Sager HB, Courties G, Dutta P, Iwamoto Y, Zaltsman A, von Zur Muhlen C, Bode C, Fricchione GL, Denninger J, Lin CP, Vinegoni C, Libby P, Swirski FK, Weissleder R, Nahrendorf M. Chronic variable stress activates hematopoietic stem cells. Nat Med 2014; 20:754-8. PubMed
  • Spencer JA, Ferraro F, Roussakis E, Klein A, Wu J, Runnels JM, Zaher W, Mortensen LJ, Alt C, Turcotte R, Yusuf R, Côté D, Vinogradov SA, Scadden DT, Lin CP. Direct measurement of local oxygen concentration in the bone marrow of live animals. Nature 2014; 508:269-73. PubMed
  • Ramsey H, Zhang Q, Brown DE, Steensma DP, Lin CP, Wu MX. Stress-induced hematopoietic failure in the absence of immediate early response gene X-1 (IEX-1, IER3). Haematologica 2014; 99:282-91. PubMed
  • Park D, Spencer JA, Lin CP, Scadden DT. Sequential in vivo imaging of osteogenic stem/progenitor cells during fracture repair. 2014. PubMed
  • Lee SK, Mortensen LJ, Lin CP, Tung CH. An authentic imaging probe to track cell fate from beginning to end. Nat Commun 2014; 5:5216. PubMed
  • Silberstein LE, Lin CP. A new image of the hematopoietic stem cell vascular niche. Cell Stem Cell 2013; 13:514-6. PubMed
  • Levy O, Zhao W, Mortensen LJ, Leblanc S, Tsang K, Fu M, Phillips JA, Sagar V, Anandakumaran P, Ngai J, Cui CH, Eimon P, Angel M, Lin CP, Yanik MF, Karp JM. mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation. Blood 2013; 122:e23-32. PubMed
  • Mortensen LJ, Levy O, Phillips JP, Stratton T, Triana B, Ruiz JP, Gu F, Karp JM, Lin CP. Quantification of Mesenchymal Stem Cell (MSC) delivery to a target site using in vivo confocal microscopy. PLoS ONE 2013; 8:e78145. PubMed
  • Carlson AL, Fujisaki J, Wu J, Runnels JM, Turcotte R, Spencer JA, Celso CL, Scadden DT, Strom TB, Lin CP. Tracking single cells in live animals using a photoconvertible near-infrared cell membrane label. PLoS ONE 2013; 8:e69257. PubMed
  • Phillips JA, Mortensen LJ, Ruiz JP, Sridharan R, Kumar S, Torres M, Sharma P, Lin CP, Karp JM, Hauschka PV. Advances in Single-cell Tracking of Mesenchymal Stem Cells (MSCs) During Musculoskeletal Regeneration. Orthop J Harv Med Sch 2012; 14:22-28. PubMed
  • Zettergren E, Swamy T, Runnels J, Lin CP, Niedre M. Tomographic sensing and localization of fluorescently labeled circulating cells in mice in vivo. Phys Med Biol 2012; 57:4627-41. PubMed
  • Fujisaki J, Wu J, Carlson AL, Silberstein L, Putheti P, Larocca R, Gao W, Saito TI, Lo Celso C, Tsuyuzaki H, Sato T, Côté D, Sykes M, Strom TB, Scadden DT, Lin CP. In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. Nature 2011; 474:216-9. PubMed
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DF/HCC members that share similar concepts* with Charles P. Lin, PhD but have yet to coauthor a publication with this researcher.

* Concepts are MeSH terms, automatically derived from member publications

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